Strain Name:
C57BL/6J-MtgxR2325Btlr/Mmmh
Stock Number:
040316-MU
Citation ID:
RRID:MMRRC_040316-MU
Other Names:
R2325 (G1), C57BL/6J-MtgxR2325Btlr
Major Collection:

Gene Information

Slc26a5
Name: solute carrier family 26, member 5
Synonyms: Pres, prestin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 80979
HGNC: HGNC:9359
Homologene: 69472
Esco2
Name: establishment of sister chromatid cohesion N-acetyltransferase 2
Synonyms: D030072L07Rik, 2410004I17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 71988
Homologene: 12432
Ppp1r37
Name: protein phosphatase 1, regulatory subunit 37
Synonyms: Lrrc68
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 232947
Homologene: 17851
Prkci
Name: protein kinase C, iota
Synonyms: Pkcl, Pkci, 2310021H13Rik, Prkcl, aPKClambda, PKClambda
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 18759
HGNC: HGNC:9404
Homologene: 37667
Vcl
Name: vinculin
Synonyms: metavinculin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 22330
VEGA: 14
Homologene: 7594
Zfp462
Name: zinc finger protein 462
Synonyms: Gt4-2, 9430078C22Rik, Zfpip
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242466
Homologene: 41430
Elp1
Name: elongator complex protein 1
Synonyms: 3110040G09Rik, C78473, IKAP, Ikbkap
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230233
HGNC: HGNC:5959
Homologene: 2699
Tmem161b
Name: transmembrane protein 161B
Synonyms: 2810446P07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 72745
Homologene: 14519
Gtf2b
Name: general transcription factor IIB
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229906
HGNC: HGNC:4648
Homologene: 1158
Scube2
Name: signal peptide, CUB domain, EGF-like 2
Synonyms: ICRFP703N2430Q5.1, ICRFP703B1614Q5.1, 4932442O19Rik, Cegf1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 56788
Homologene: 36383
B3galt2
Name: UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 26878
HGNC: HGNC:917
Homologene: 74512
Atp2b1
Name: ATPase, Ca++ transporting, plasma membrane 1
Synonyms: PMCA1, E130111D10Rik, 2810442I22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 67972
VEGA: 10
HGNC: HGNC:814
Homologene: 55597
Cdh9
Name: cadherin 9
Synonyms: T1-cadherin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 12565
HGNC: HGNC:1768
Homologene: 9450
Cyp4x1
Name: cytochrome P450, family 4, subfamily x, polypeptide 1
Synonyms: Cyp4a28-ps
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 81906
Homologene: 75853
N4bp1
Name: NEDD4 binding protein 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 80750
Homologene: 23703
Gin1
Name: gypsy retrotransposon integrase 1
Synonyms: 4930429M06, 4930429M06Rik, Zh2c2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 252876
Homologene: 69238
Dse
Name: dermatan sulfate epimerase
Synonyms: B130024B19Rik, Sart2, DS-epi1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 212898
VEGA: 10
Homologene: 8354
Prss35
Name: protease, serine 35
Synonyms: 6030424L22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 244954
Homologene: 17734
Lct
Name: lactase
Synonyms: LOC226413, Lphl, LPH
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226413
HGNC: HGNC:6530
Homologene: 124204
Ell2
Name: elongation factor for RNA polymerase II 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 192657
VEGA: 13
Homologene: 8094
Med12l
Name: mediator complex subunit 12-like
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 329650
Homologene: 43143
Rasef
Name: RAS and EF hand domain containing
Synonyms: RAB45
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242505
Homologene: 28424
Tmem132e
Name: transmembrane protein 132E
Synonyms: LOC270893
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 270893
Homologene: 41524
Elac1
Name: elaC ribonuclease Z 1
Synonyms: 8430417G19Rik, 2610018O07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 114615
Homologene: 10272
4921528O07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Stil
Name: Scl/Tal1 interrupting locus
Synonyms: Sil
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 20460
Homologene: 2283
Tmem273
Name: transmembrane protein 273
Synonyms: 1810011H11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 69069
VEGA: 14
Homologene: 78635
Nebl
Name: nebulette
Synonyms: Lnebl, 1200007O21Rik, A630080F05Rik, D830029A09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 74103
Homologene: 128431
Thbs2
Name: thrombospondin 2
Synonyms: TSP2, Thbs-2, Thrombospondin-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 21826
VEGA: 17
Homologene: 2438
Kcnh6
Name: potassium voltage-gated channel, subfamily H (eag-related), member 6
Synonyms: m-erg2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 192775
Homologene: 32740
Prmt8
Name: protein arginine N-methyltransferase 8
Synonyms: Hrmt1l3, Hrmt1l4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 381813
HGNC: HGNC:5188
Homologene: 76124
Zc3h15
Name: zinc finger CCCH-type containing 15
Synonyms: FM22, Ierepo4, 1700006A17Rik, 1810012H02Rik, 2610312B22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 69082
Homologene: 10216
Akr1c20
Name: aldo-keto reductase family 1, member C20
Synonyms: 2610528B18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 116852
Vmn1r173
Name: vomeronasal 1 receptor 173
Synonyms: Gm5892
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 545934
Homologene: 79577
P2ry1
Name: purinergic receptor P2Y, G-protein coupled 1
Synonyms: P2Y1, P2Y1 receptor, P2y1r
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 18441
HGNC: HGNC:8539
Homologene: 1926
Lrp8
Name: low density lipoprotein receptor-related protein 8, apolipoprotein e receptor
Synonyms: apoER2, 4932703M08Rik, Lr8b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 16975
HGNC: HGNC:6700
Homologene: 31250
Fam170a
Name: family with sequence similarity 170, member A
Synonyms: LOC225497, Znfd
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 225497
VEGA: 18
Homologene: 86702
Lrriq3
Name: leucine-rich repeats and IQ motif containing 3
Synonyms: 4930438B07Rik, 4930511J15Rik, 4933403H06Rik, Lrrc44
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 74435
Homologene: 23668
Csrp3
Name: cysteine and glycine-rich protein 3
Synonyms: CRP3, muscle LIM protein, MLP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 13009
HGNC: HGNC:2472
Homologene: 20742
Gm8439
Name: predicted gene 8439
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 667063
VEGA: 4
Atoh7
Name: atonal bHLH transcription factor 7
Synonyms: Math5, bHLHa13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 53404
VEGA: 10
Homologene: 32338
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to C, chromosome 1 at 97,792,561 bp
  • T to C, chromosome 1 at 128,304,226 bp
  • A to G, chromosome 1 at 143,647,188 bp
  • A to C, chromosome 1 at 163,026,530 bp
  • T to C, chromosome 2 at 17,393,016 bp
  • G to A, chromosome 2 at 83,653,439 bp
  • T to A, chromosome 3 at 31,031,068 bp
  • A to G, chromosome 3 at 59,232,454 bp
  • A to T, chromosome 3 at 61,003,578 bp
  • A to G, chromosome 3 at 142,780,090 bp
  • G to A, chromosome 3 at 155,163,900 bp
  • A to C, chromosome 4 at 55,013,712 bp
  • A to T, chromosome 4 at 56,784,622 bp
  • A to G, chromosome 4 at 73,749,972 bp
  • A to T, chromosome 4 at 107,864,009 bp
  • T to A, chromosome 4 at 115,032,707 bp
  • A to T, chromosome 4 at 115,124,379 bp
  • A to G, chromosome 4 at 120,588,851 bp
  • T to G, chromosome 5 at 21,819,694 bp
  • T to C, chromosome 6 at 127,729,407 bp
  • A to T, chromosome 7 at 19,532,684 bp
  • T to A, chromosome 7 at 23,703,112 bp
  • T to A, chromosome 7 at 48,833,882 bp
  • C to G, chromosome 7 at 109,843,954 bp
  • T to C, chromosome 8 at 86,848,460 bp
  • G to A, chromosome 9 at 86,756,304 bp
  • A to T, chromosome 10 at 34,184,047 bp
  • C to A, chromosome 10 at 63,100,145 bp
  • C to T, chromosome 10 at 99,018,895 bp
  • C to A, chromosome 11 at 82,434,515 bp
  • C to T, chromosome 11 at 106,033,835 bp
  • T to C, chromosome 13 at 4,523,296 bp
  • T to A, chromosome 13 at 75,769,626 bp
  • C to A, chromosome 13 at 84,294,768 bp
  • T to A, chromosome 14 at 21,010,176 bp
  • G to T, chromosome 14 at 32,808,385 bp
  • A to T, chromosome 14 at 65,826,578 bp
  • A to G, chromosome 15 at 16,778,200 bp
  • A to T, chromosome 17 at 14,690,289 bp
  • A to G, chromosome 18 at 50,281,850 bp
  • T to C, chromosome 18 at 73,739,313 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2325 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040316-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.