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Strain Name:
C57BL/6J-MtgxR2392Btlr/Mmmh
Stock Number:
040360-MU
Citation ID:
RRID:MMRRC_040360-MU
Other Names:
R2392 (G1), C57BL/6J-MtgxR2392Btlr
Major Collection:

Strain Information

Myo5a
Name: myosin VA
Synonyms: MVa, MyoVA, Myo5, flail, 9630007J19Rik, Dbv
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17918
HGNC: HGNC:7602
Homologene: 20100
Nes
Name: nestin
Synonyms: RC2, Marc2, ESTM46, Ifaprc2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18008
HGNC: HGNC:7756
Homologene: 136487
Abca12
Name: ATP-binding cassette, sub-family A member 12
Synonyms: 4832428G11Rik, 4833417A11Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 74591
Homologene: 45441
Jmjd1c
Name: jumonji domain containing 1C
Synonyms: TRIP8, 5430433L24Rik, D630035I23Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 108829
Homologene: 3129
Nr4a1
Name: nuclear receptor subfamily 4, group A, member 1
Synonyms: Nur77, TIS1, TR3, N10, NP10, GFRP1, NGFI-B, Hbr-1, Gfrp, Hbr1, Hmr
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 15370
VEGA: 15
HGNC: HGNC:7980
Homologene: 1612
Acap2
Name: ArfGAP with coiled-coil, ankyrin repeat and PH domains 2
Synonyms: 9530039J15Rik, Centb2
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 78618
VEGA: 16
Homologene: 8182
Kif1b
Name: kinesin family member 1B
Synonyms: D4Mil1e, Kif1b alpha, Kif1b beta, KIF1Bp130, KIF1Bp204, N-3 kinesin, A530096N05Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 16561
Homologene: 99835
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 37,498,704 bp
  • A to T, chromosome 1 at 71,258,105 bp
  • G to A, chromosome 1 at 87,386,900 bp
  • A to T, chromosome 1 at 93,354,341 bp
  • G to A, chromosome 1 at 183,087,167 bp
  • C to A, chromosome 2 at 24,394,667 bp
  • A to G, chromosome 2 at 66,207,450 bp
  • T to C, chromosome 2 at 121,029,987 bp
  • T to A, chromosome 2 at 144,585,587 bp
  • T to A, chromosome 3 at 14,536,520 bp
  • A to G, chromosome 3 at 65,948,752 bp
  • C to T, chromosome 3 at 87,975,943 bp
  • T to G, chromosome 3 at 135,248,113 bp
  • A to C, chromosome 3 at 138,095,021 bp
  • T to A, chromosome 4 at 3,943,381 bp
  • C to G, chromosome 4 at 121,054,258 bp
  • A to G, chromosome 4 at 129,433,189 bp
  • C to T, chromosome 4 at 130,312,387 bp
  • G to T, chromosome 4 at 134,072,614 bp
  • G to A, chromosome 4 at 149,220,620 bp
  • G to T, chromosome 5 at 120,527,732 bp
  • C to T, chromosome 5 at 120,527,733 bp
  • A to G, chromosome 5 at 135,156,514 bp
  • T to C, chromosome 5 at 135,742,038 bp
  • A to G, chromosome 5 at 149,580,670 bp
  • T to G, chromosome 6 at 23,302,526 bp
  • C to A, chromosome 6 at 117,924,868 bp
  • A to C, chromosome 6 at 141,839,673 bp
  • A to T, chromosome 7 at 5,484,131 bp
  • G to A, chromosome 7 at 99,473,824 bp
  • A to G, chromosome 8 at 110,889,724 bp
  • A to G, chromosome 9 at 9,016,238 bp
  • T to C, chromosome 9 at 75,209,239 bp
  • A to T, chromosome 9 at 119,067,560 bp
  • A to G, chromosome 9 at 119,627,202 bp
  • G to A, chromosome 10 at 11,390,997 bp
  • A to G, chromosome 10 at 23,961,274 bp
  • A to G, chromosome 10 at 67,229,904 bp
  • A to T, chromosome 10 at 79,274,682 bp
  • A to G, chromosome 10 at 93,025,011 bp
  • T to C, chromosome 11 at 69,975,102 bp
  • A to G, chromosome 11 at 83,065,328 bp
  • A to G, chromosome 11 at 83,185,422 bp
  • C to A, chromosome 11 at 107,072,747 bp
  • A to G, chromosome 11 at 120,719,539 bp
  • T to C, chromosome 12 at 4,865,164 bp
  • T to A, chromosome 12 at 44,311,504 bp
  • A to G, chromosome 13 at 4,434,478 bp
  • A to G, chromosome 13 at 19,688,943 bp
  • G to A, chromosome 13 at 24,706,223 bp
  • T to A, chromosome 13 at 36,943,997 bp
  • C to A, chromosome 15 at 78,294,331 bp
  • A to G, chromosome 15 at 101,274,194 bp
  • A to G, chromosome 15 at 101,756,801 bp
  • A to G, chromosome 16 at 31,139,640 bp
  • A to T, chromosome 16 at 35,106,378 bp
  • A to G, chromosome 16 at 58,872,434 bp
  • T to A, chromosome 16 at 88,825,709 bp
  • A to G, chromosome 17 at 37,466,419 bp
  • T to A, chromosome 17 at 66,149,973 bp
  • G to T, chromosome 19 at 26,640,650 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2392 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040360-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.