Strain Name:
C57BL/6J-MtgxR2408Btlr/Mmmh
Stock Number:
040374-MU
Citation ID:
RRID:MMRRC_040374-MU
Other Names:
R2408 (G1), C57BL/6J-MtgxR2408Btlr
Major Collection:

Gene Information

Rptor
Name: regulatory associated protein of MTOR, complex 1
Synonyms: raptor, Rap, 4932417H02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 74370
Homologene: 80210
Med26
Name: mediator complex subunit 26
Synonyms: Crsp7, 5730493L18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 70625
HGNC: HGNC:2376
Homologene: 68417
Adgrg1
Name: adhesion G protein-coupled receptor G1
Synonyms: Cyt28, Gpr56
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 14766
HGNC: HGNC:4512
Homologene: 4156
Ubap2l
Name: ubiquitin-associated protein 2-like
Synonyms: 3110083O19Rik, A430103N23Rik, 4932431F02Rik, NICE-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 74383
Homologene: 136291
Nsf
Name: N-ethylmaleimide sensitive fusion protein
Synonyms: N-ethylmaleimide sensitive factor, SKD2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 18195
HGNC: HGNC:8016
Homologene: 4502
Ccdc191
Name: coiled-coil domain containing 191
Synonyms: 2610015P09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 212153
Homologene: 19484
Hnf1a
Name: HNF1 homeobox A
Synonyms: LFB1, hepatocyte nuclear factor 1, HNF1-alpha, Tcf1, HNF1[a], HNF1, Hnf1alpha, Hnf-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 21405
Homologene: 459
Fam129b
Name: family with sequence similarity 129, member B
Synonyms: 9130404D14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 227737
Homologene: 11269
Ucn3
Name: urocortin 3
Synonyms: Urocortin III
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 83428
VEGA: 13
Homologene: 49959
Prmt2
Name: protein arginine N-methyltransferase 2
Synonyms: Hrmt1l1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 15468
HGNC: HGNC:5186
Homologene: 55587
Noct
Name: nocturnin
Synonyms: Ccr4, Ccrn4l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 12457
Homologene: 7661
Kalrn
Name: kalirin, RhoGEF kinase
Synonyms: LOC224126, Hapip, E530005C20Rik, 2210407G14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 545156
HGNC: HGNC:4814
Homologene: 57160
Nbl1
Name: NBL1, DAN family BMP antagonist
Synonyms: NO3, Dana, DAN, D4H1S1733E
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 17965
Homologene: 3924
Ttc16
Name: tetratricopeptide repeat domain 16
Synonyms: 1200002K10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 338348
Homologene: 17011
Col7a1
Name: collagen, type VII, alpha 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 12836
HGNC: HGNC:2214
Homologene: 73
Mgam
Name: maltase-glucoamylase
Synonyms: 6030407P20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232714
HGNC: HGNC:7043
Homologene: 130099
Slc13a5
Name: solute carrier family 13 (sodium-dependent citrate transporter), member 5
Synonyms: NaC2/NaCT, mINDY, Indy, Nact
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237831
Homologene: 21941
Vwa3a
Name: von Willebrand factor A domain containing 3A
Synonyms: E030013G06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233813
Homologene: 18607
Sycp1
Name: synaptonemal complex protein 1
Synonyms: SCP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 20957
Homologene: 2389
Ac127374.1
Name: Uncharacterized protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Trmt1l
Name: tRNA methyltransferase 1 like
Synonyms: Trm1-like, 1190005F20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 98685
Homologene: 12801
Hhipl1
Name: hedgehog interacting protein-like 1
Synonyms: 1600002O04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 214305
VEGA: 12
Homologene: 81985
Gm10782
Name: predicted gene 10782
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 100038494
VEGA: 13
Dennd4b
Name: DENN/MADD domain containing 4B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229541
Homologene: 28281
Msh5
Name: mutS homolog 5
Synonyms: G7, Mut5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 17687
HGNC: HGNC:7328
Homologene: 8415
BC051076
Name: cDNA sequence BC051076
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 243090
Exd2
Name: exonuclease 3'-5' domain containing 2
Synonyms: Exdl2, 4930539P14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 97827
VEGA: 12
Homologene: 10066
Zmynd19
Name: zinc finger, MYND domain containing 19
Synonyms: 2700064H14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 67187
Homologene: 12093
Tmem222
Name: transmembrane protein 222
Synonyms: D4Ertd196e, 5730406H10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 52174
Homologene: 11999
AC133939.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Rasgrf2
Name: RAS protein-specific guanine nucleotide-releasing factor 2
Synonyms: 6330417G04Rik, Grf2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 19418
HGNC: HGNC:9876
Homologene: 2169
Ifi204
Name: interferon activated gene 204
Synonyms: p204
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 15951
HGNC: HGNC:5395
Homologene: 115929
Sgms1
Name: sphingomyelin synthase 1
Synonyms: 9530058O11Rik, Tmem23, SMS1alpha1, SMS1alpha2, SMS1beta, SMS1gamma, SMS1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 208449
Homologene: 27040
Dusp7
Name: dual specificity phosphatase 7
Synonyms: MKP-X, PYST2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235584
HGNC: HGNC:3073
Homologene: 1468
Fxyd1
Name: FXYD domain-containing ion transport regulator 1
Synonyms: PLM, phospholemman, 1110006M24Rik, PML, 0610012C17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 56188
HGNC: HGNC:4025
Homologene: 3691
Dipk1a
Name: divergent protein kinase domain 1A
Synonyms: 2900024C23Rik, Fam69a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 67266
Homologene: 12104
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 151,439,516 bp
  • A to G, chromosome 1 at 173,755,632 bp
  • A to G, chromosome 2 at 24,958,925 bp
  • A to G, chromosome 2 at 32,768,008 bp
  • A to G, chromosome 2 at 32,923,470 bp
  • T to C, chromosome 3 at 51,225,289 bp
  • T to C, chromosome 3 at 90,009,132 bp
  • G to T, chromosome 3 at 90,271,575 bp
  • T to C, chromosome 3 at 102,925,259 bp
  • T to C, chromosome 4 at 133,271,024 bp
  • C to T, chromosome 4 at 139,083,532 bp
  • A to T, chromosome 5 at 7,179,410 bp
  • C to T, chromosome 5 at 87,963,825 bp
  • T to C, chromosome 5 at 107,914,425 bp
  • C to T, chromosome 5 at 114,960,011 bp
  • T to A, chromosome 6 at 40,686,522 bp
  • G to A, chromosome 7 at 31,053,878 bp
  • T to C, chromosome 7 at 120,773,294 bp
  • T to C, chromosome 8 at 31,737,278 bp
  • T to C, chromosome 8 at 72,495,632 bp
  • T to G, chromosome 8 at 95,003,493 bp
  • C to T, chromosome 9 at 106,369,162 bp
  • A to G, chromosome 9 at 108,978,021 bp
  • A to T, chromosome 10 at 76,208,467 bp
  • A to G, chromosome 11 at 72,262,076 bp
  • C to T, chromosome 11 at 103,930,752 bp
  • A to G, chromosome 11 at 119,857,451 bp
  • T to G, chromosome 12 at 80,484,241 bp
  • T to A, chromosome 12 at 108,318,547 bp
  • T to G, chromosome 13 at 3,941,413 bp
  • C to A, chromosome 13 at 56,363,131 bp
  • T to C, chromosome 13 at 91,968,935 bp
  • C to T, chromosome 16 at 33,989,810 bp
  • A to T, chromosome 16 at 43,931,198 bp
  • T to C, chromosome 17 at 35,045,119 bp
  • G to A, chromosome 19 at 32,159,672 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2408 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040374-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.