Strain Name:
C57BL/6J-MtgxR2424Btlr/Mmmh
Stock Number:
040386-MU
Citation ID:
RRID:MMRRC_040386-MU
Other Names:
R2424 (G1), C57BL/6J-MtgxR2424Btlr
Major Collection:

Gene Information

Kit
Name: KIT proto-oncogene receptor tyrosine kinase
Synonyms: Gsfsco5, Dominant white spotting, Tr-kit, belly-spot, Steel Factor Receptor, c-KIT, Gsfsow3, Gsfsco1, CD117, SOW3, SCO5, SCO1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 16590
HGNC: HGNC:6342
Homologene: 187
Eya1
Name: EYA transcriptional coactivator and phosphatase 1
Synonyms: bor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 14048
HGNC: HGNC:3519
Homologene: 74943
Kcnj5
Name: potassium inwardly-rectifying channel, subfamily J, member 5
Synonyms: Kir3.4, GIRK4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 16521
VEGA: 9
HGNC: HGNC:6266
Homologene: 20248
Dst
Name: dystonin
Synonyms: Bpag1, Bpag, 2310001O04Rik, nmf339, BPAG1, BPAG1-n, bullous pemphigoid antigen 1, Macf2, nmf203, bullous pemphigoid antigen 1, athetoid, A830042E19Rik, ah
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 13518
HGNC: HGNC:1090
Homologene: 136716
Capzb
Name: capping protein (actin filament) muscle Z-line, beta
Synonyms: CPB1, CPbeta2, CPB2, 1700120C01Rik, Cappb1, CPbeta1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 12345
HGNC: HGNC:1491
Homologene: 3620
Ctnna1
Name: catenin (cadherin associated protein), alpha 1
Synonyms: Catna1, alpha E catenin, alpha(E)-catenin, 2010010M04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 12385
VEGA: 18
HGNC: HGNC:2509
Homologene: 1433
Mrpl39
Name: mitochondrial ribosomal protein L39
Synonyms: MRP-L5, Rpml5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 27393
Homologene: 9679
Sema4b
Name: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B
Synonyms: Semac, SemC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 20352
Homologene: 8426
Phf3
Name: PHD finger protein 3
Synonyms: 2310061N19Rik, AU020177
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 213109
HGNC: HGNC:8921
Homologene: 9040
Ascc3
Name: activating signal cointegrator 1 complex subunit 3
Synonyms: B630009I04Rik, Helic1, ASC1p200
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 77987
VEGA: 10
Homologene: 4973
Setd2
Name: SET domain containing 2
Synonyms: 4921524K10Rik, KMT3A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235626
Homologene: 56493
Papola
Name: poly (A) polymerase alpha
Synonyms: PapIII, Plap
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 18789
Homologene: 23389
Urb2
Name: URB2 ribosome biogenesis 2 homolog (S. cerevisiae)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 382038
Homologene: 8859
Dpp3
Name: dipeptidylpeptidase 3
Synonyms: 4930533O14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 75221
VEGA: 19
HGNC: HGNC:3008
Homologene: 40210
Ago3
Name: argonaute RISC catalytic subunit 3
Synonyms: argonaute 3, eIF2C3, C130014L07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 214150
Homologene: 49799
Supt5
Name: suppressor of Ty 5, DSIF elongation factor subunit
Synonyms: Supt5h, Spt5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 20924
Homologene: 2384
Phc1
Name: polyhomeotic 1
Synonyms: Mph1, Rae-28, Edr1, rae28
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 13619
HGNC: HGNC:3182
Homologene: 107079
Tbp
Name: TATA box binding protein
Synonyms: Gtf2d
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 21374
Homologene: 2404
Akap9
Name: A kinase (PRKA) anchor protein (yotiao) 9
Synonyms: repro12, mei2-5, G1-448-15, AKAP450, 5730481H23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100986
HGNC: HGNC:379
Homologene: 134583
Usp24
Name: ubiquitin specific peptidase 24
Synonyms: 2810030C21Rik, 2700066K03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 329908
Homologene: 35420
Dab1
Name: disabled 1
Synonyms: C630028C02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 13131
HGNC: HGNC:2661
Homologene: 32084
Dock7
Name: dedicator of cytokinesis 7
Synonyms: m, 3110056M06Rik, LOC242555
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 67299
Homologene: 23566
Epg5
Name: ectopic P-granules autophagy protein 5 homolog (C. elegans)
Synonyms: 5430411K18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 100502841
VEGA: 18
Homologene: 14575
Cdh9
Name: cadherin 9
Synonyms: T1-cadherin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 12565
HGNC: HGNC:1768
Homologene: 9450
Lama1
Name: laminin, alpha 1
Synonyms: Lama
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 16772
VEGA: 17
HGNC: HGNC:6481
Homologene: 21146
Stim1
Name: stromal interaction molecule 1
Synonyms: SIM
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 20866
Homologene: 20681
Mapk9
Name: mitogen-activated protein kinase 9
Synonyms: JNK/SAPK alpha, c-Jun N-terminal kinase, JNK2, Prkm9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 26420
HGNC: HGNC:6886
Homologene: 55685
Rnf214
Name: ring finger protein 214
Synonyms: D130054N24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235315
Homologene: 109357
Plxna2
Name: plexin A2
Synonyms: Plxn2, OCT, PlexA2, 2810428A13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 18845
HGNC: HGNC:9100
Homologene: 56427
Grin1
Name: glutamate receptor, ionotropic, NMDA1 (zeta 1)
Synonyms: Nmdar, NR1, M100174, GluRzeta1, NMDAR1, Rgsc174
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14810
HGNC: HGNC:4584
Homologene: 7187
Atp10a
Name: ATPase, class V, type 10A
Synonyms: Atp10c, pfatp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 11982
Homologene: 56461
Ttn
Name: titin
Synonyms: mdm, 2310036G12Rik, D830007G01Rik, shru, L56, 2310074I15Rik, 2310057K23Rik, connectin, D330041I19Rik, 1100001C23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Mybpc3
Name: myosin binding protein C, cardiac
Synonyms: cardiac C-protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 17868
HGNC: HGNC:7551
Homologene: 215
Il11ra1
Name: interleukin 11 receptor, alpha chain 1
Synonyms: Il-11ra, Il11ra, Il-11ra-alpha, NR1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 16157
HGNC: HGNC:5967
Homologene: 88705
Neb
Name: nebulin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 17996
HGNC: HGNC:7720
Homologene: 136285
Cacna1d
Name: calcium channel, voltage-dependent, L type, alpha 1D subunit
Synonyms: Cacnl1a2, Cchl1a2, Cchl1a, 8430418G19Rik, D-LTCC, C79217, Cav1.3alpha1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 12289
VEGA: 14
HGNC: HGNC:1391
Homologene: 578
Fbn2
Name: fibrillin 2
Synonyms: sy, Fib-2, Sne
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 14119
VEGA: 18
HGNC: HGNC:3604
Homologene: 1515
Madd
Name: MAP-kinase activating death domain
Synonyms: 9630059K23Rik, Rab3 GEP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228355
HGNC: HGNC:6766
Homologene: 14249
Otog
Name: otogelin
Synonyms: Otgn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 18419
HGNC: HGNC:8516
Homologene: 8421
Obscn
Name: obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF
Synonyms: LOC380698, OTTMUSG00000005786
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 380698
Homologene: 70869
Kif21a
Name: kinesin family member 21A
Synonyms: N-5 kinesin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 16564
VEGA: 15
Homologene: 56761
Phf8-ps
Name: PHD finger protein 8, pseudogene
Synonyms: 4921501E09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 74042
VEGA: 17
Homologene: 66275
Kprp
Name: keratinocyte expressed, proline-rich
Synonyms: 1110001M24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 433619
Homologene: 54921
Arid5a
Name: AT rich interactive domain 5A (MRF1-like)
Synonyms: Mrf1, D430024K22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 214855
Homologene: 34940
Vmn2r72
Name: vomeronasal 2, receptor 72
Synonyms: EG244114, Vmn2r72-ps
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 244114
Homologene: 115466
Olfr904
Name: olfactory receptor 904
Synonyms: GA_x6K02T2PVTD-32156773-32157705, MOR167-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258797
VEGA: 9
Homologene: 133629
Slc27a6
Name: solute carrier family 27 (fatty acid transporter), member 6
Synonyms: FATP6, 4732438L20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 225579
Homologene: 38385
Pde1a
Name: phosphodiesterase 1A, calmodulin-dependent
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 18573
HGNC: HGNC:8774
Homologene: 21043
Rgs17
Name: regulator of G-protein signaling 17
Synonyms: 6430507P11Rik, RGSZ2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 56533
Homologene: 8242
Vmn2r114
Name: vomeronasal 2, receptor 114
Synonyms: EG666002
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 666002
Homologene: 86604
Tmem132d
Name: transmembrane protein 132D
Synonyms: C630028F04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 243274
Homologene: 71684
Fbxw21
Name: F-box and WD-40 domain protein 21
Synonyms: E330009P21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 320082
Homologene: 110776
Vmn2r91
Name: vomeronasal 2, receptor 91
Synonyms: EG665210
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 665210
Homologene: 115024
Cst7
Name: cystatin F (leukocystatin)
Synonyms: CMAP, Leukocystatin, cystatin-like metastasis-associated protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 13011
HGNC: HGNC:2479
Homologene: 37832
Aacs
Name: acetoacetyl-CoA synthetase
Synonyms: 2210408B16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 78894
Homologene: 11322
Olfr725
Name: olfactory receptor 725
Synonyms: MOR246-3, GA_x6K02T2PMLR-5725741-5724776, MOR246-7_p
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 258314
Homologene: 44957
Ngly1
Name: N-glycanase 1
Synonyms: Png1, PNGase, 1110002C09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 59007
Homologene: 10117
Mrpl9
Name: mitochondrial ribosomal protein L9
Synonyms: 8030480E20Rik, C330013D18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 78523
Homologene: 12696
Btbd6
Name: BTB (POZ) domain containing 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 399566
VEGA: 12
Homologene: 41967
Acot3
Name: acyl-CoA thioesterase 3
Synonyms: Pte2a, PTE-Ia
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 171281
Homologene: 134585
Olfr92
Name: olfactory receptor 92
Synonyms: MOR256-29, GA_x6K02T2PSCP-1552066-1551128
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 258448
HGNC: HGNC:8253
Homologene: 115562
Haao
Name: 3-hydroxyanthranilate 3,4-dioxygenase
Synonyms: 3HAO, 3-HAO, 3-HAOxase, 0610012J07Rik, 0610007K21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 107766
VEGA: 17
HGNC: HGNC:4796
Homologene: 8148
Nt5c1b
Name: 5'-nucleotidase, cytosolic IB
Synonyms: 4921514H13Rik, CN-IB
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 70881
Homologene: 12370
Fam187a
Name: family with sequence similarity 187, member A
Synonyms: 4933439F11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 66784
Homologene: 128440
Olfr968
Name: olfactory receptor 968
Synonyms: GA_x6K02T2PVTD-33470347-33469403, MOR171-15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258605
VEGA: 9
Homologene: 121532
Rnase1
Name: ribonuclease, RNase A family, 1 (pancreatic)
Synonyms: Rib-1, Rib1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 19752
VEGA: 14
Homologene: 7919
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 14,270,848 bp
  • G to T, chromosome 1 at 30,806,349 bp
  • T to A, chromosome 1 at 34,167,060 bp
  • T to C, chromosome 1 at 36,318,501 bp
  • C to T, chromosome 1 at 194,749,317 bp
  • A to T, chromosome 2 at 25,318,652 bp
  • A to G, chromosome 2 at 52,209,659 bp
  • T to C, chromosome 2 at 76,881,145 bp
  • A to T, chromosome 2 at 79,898,191 bp
  • A to T, chromosome 2 at 91,135,793 bp
  • G to C, chromosome 2 at 91,166,622 bp
  • G to T, chromosome 2 at 150,570,597 bp
  • A to T, chromosome 3 at 92,825,605 bp
  • T to C, chromosome 3 at 94,443,806 bp
  • T to A, chromosome 4 at 41,768,222 bp
  • T to A, chromosome 4 at 98,945,307 bp
  • C to T, chromosome 4 at 104,731,751 bp
  • T to C, chromosome 4 at 106,399,113 bp
  • A to G, chromosome 4 at 126,404,247 bp
  • A to G, chromosome 4 at 139,194,130 bp
  • A to C, chromosome 5 at 4,065,279 bp
  • T to A, chromosome 5 at 75,648,375 bp
  • A to G, chromosome 5 at 125,513,095 bp
  • A to G, chromosome 5 at 127,864,599 bp
  • A to G, chromosome 6 at 122,320,043 bp
  • T to C, chromosome 7 at 28,315,165 bp
  • A to T, chromosome 7 at 46,298,169 bp
  • A to T, chromosome 7 at 58,794,555 bp
  • A to G, chromosome 7 at 80,219,275 bp
  • A to G, chromosome 7 at 85,750,953 bp
  • A to G, chromosome 7 at 102,408,405 bp
  • T to A, chromosome 8 at 124,030,426 bp
  • A to T, chromosome 9 at 32,322,820 bp
  • T to C, chromosome 9 at 38,464,832 bp
  • A to G, chromosome 9 at 39,772,297 bp
  • T to C, chromosome 9 at 45,899,798 bp
  • A to T, chromosome 9 at 109,157,519 bp
  • A to G, chromosome 9 at 110,617,522 bp
  • T to A, chromosome 10 at 5,833,111 bp
  • T to A, chromosome 10 at 5,842,560 bp
  • T to C, chromosome 10 at 50,618,201 bp
  • A to G, chromosome 11 at 49,863,672 bp
  • T to C, chromosome 11 at 58,994,451 bp
  • T to C, chromosome 11 at 102,885,954 bp
  • A to T, chromosome 12 at 10,370,072 bp
  • G to T, chromosome 12 at 84,053,864 bp
  • A to G, chromosome 12 at 105,827,052 bp
  • A to G, chromosome 12 at 112,978,360 bp
  • A to G, chromosome 14 at 16,290,721 bp
  • A to T, chromosome 14 at 30,049,023 bp
  • T to C, chromosome 14 at 50,034,824 bp
  • A to G, chromosome 14 at 51,145,547 bp
  • T to G, chromosome 15 at 16,850,354 bp
  • T to A, chromosome 15 at 90,971,196 bp
  • A to G, chromosome 16 at 84,730,860 bp
  • T to C, chromosome 17 at 15,513,533 bp
  • C to A, chromosome 17 at 18,136,169 bp
  • T to C, chromosome 17 at 23,296,868 bp
  • T to C, chromosome 17 at 33,065,756 bp
  • T to C, chromosome 17 at 37,111,516 bp
  • C to T, chromosome 17 at 67,798,665 bp
  • T to A, chromosome 17 at 83,835,562 bp
  • T to C, chromosome 18 at 35,253,707 bp
  • C to T, chromosome 18 at 58,203,787 bp
  • G to T, chromosome 18 at 58,605,117 bp
  • T to C, chromosome 18 at 77,968,613 bp
  • A to T, chromosome 19 at 4,907,707 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2424 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040386-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.