Strain Name:
C57BL/6J-MtgxR2428Btlr/Mmmh
Stock Number:
040390-MU
Citation ID:
RRID:MMRRC_040390-MU
Other Names:
R2428 (G1), C57BL/6J-MtgxR2428Btlr
Major Collection:

Strain Information

Igf1
Name: insulin-like growth factor 1
Synonyms: Igf-1, Igf-I, C730016P09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 16000
HGNC: HGNC:5464
Homologene: 515
Prrc2b
Name: proline-rich coiled-coil 2B
Synonyms: 5830434P21Rik, Bat2l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 227723
Homologene: 106649
Zdbf2
Name: zinc finger, DBF-type containing 2
Synonyms: 9330107J05Rik, 4930431J08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 73884
Homologene: 52868
Dmtn
Name: dematin actin binding protein
Synonyms: dematin, Epb4.9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 13829
VEGA: 14
HGNC: HGNC:3382
Homologene: 1496
Ctc1
Name: CTS telomere maintenance complex component 1
Synonyms: 1500010J02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 68964
Homologene: 11830
Relch
Name: RAB11 binding and LisH domain, coiled-coil and HEAT repeat containing
Synonyms: 2310035C23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 227446
Homologene: 10834
Ptpn11
Name: protein tyrosine phosphatase, non-receptor type 11
Synonyms: PTP2C, PTP1D, SH-PTP2, SHP-2, Syp, Shp2, SH2 domain-containing protein tyrosine phosphatase-2, 2700084A17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 19247
HGNC: HGNC:9644
Homologene: 2122
F2rl2
Name: coagulation factor II (thrombin) receptor-like 2
Synonyms: PAR3, PAR-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 14064
HGNC: HGNC:3539
Homologene: 36151
Lrch1
Name: leucine-rich repeats and calponin homology (CH) domain containing 1
Synonyms: 4832412D13Rik, Chdc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 380916
VEGA: 14
Homologene: 32244
Cubn
Name: cubilin (intrinsic factor-cobalamin receptor)
Synonyms: D2Wsu88e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 65969
HGNC: HGNC:2548
Homologene: 37434
Sppl2a
Name: signal peptide peptidase like 2A
Synonyms: C130089K23Rik, 2010106G01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 66552
Homologene: 36411
Tespa1
Name: thymocyte expressed, positive selection associated 1
Synonyms: A430001F24Rik, 5830405N20Rik, Itprid3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 67596
VEGA: 10
Homologene: 106645
Ttn
Name: titin
Synonyms: connectin, L56, mdm, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, shru
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Col12a1
Name: collagen, type XII, alpha 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 12816
HGNC: HGNC:2188
Homologene: 3217
Pkdrej
Name: polycystin (PKD) family receptor for egg jelly
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 18766
VEGA: 15
HGNC: HGNC:9015
Homologene: 4427
Or4f62
Name: olfactory receptor family 4 subfamily F member 62
Synonyms: GA_x6K02T2Q125-73202172-73203134, MOR245-16, Olfr1318
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258022
Homologene: 134079
Astn1
Name: astrotactin 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 11899
HGNC: HGNC:773
Homologene: 7233
Wdr53
Name: WD repeat domain 53
Synonyms: 1500002B03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 68980
Homologene: 15591
Acad9
Name: acyl-Coenzyme A dehydrogenase family, member 9
Synonyms: NPD002, 2600017P15Rik, C630012L17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229211
Homologene: 8539
Or6c212
Name: olfactory receptor family 6 subfamily C member 212
Synonyms: GA_x6K02T2PULF-11402237-11401278, MOR110-4, Olfr805
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 258548
Plxnb1
Name: plexin B1
Synonyms: 2900002G15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235611
HGNC: HGNC:9103
Homologene: 130508
Tmcc2
Name: transmembrane and coiled-coil domains 2
Synonyms: 1110063G11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 68875
Homologene: 8905
Bag6
Name: BCL2-associated athanogene 6
Synonyms: D17H6S52E, G3, 2410045D21Rik, Scythe, Bat3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224727
Homologene: 3409
Nfasc
Name: neurofascin
Synonyms: D430023G06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 269116
Homologene: 24945
Hivep3
Name: human immunodeficiency virus type I enhancer binding protein 3
Synonyms: Krc, 2900056N03Rik, E030045D18Rik, Shn3, Schnurri-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 16656
Homologene: 7803
Atpaf1
Name: ATP synthase mitochondrial F1 complex assembly factor 1
Synonyms: ATP11, ATP11p, 6330547J17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230649
Homologene: 41492
Or51a43
Name: olfactory receptor family 51 subfamily A member 43
Synonyms: GA_x6K02T2PBJ9-6803062-6802118, MOR13-1, Olfr644
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 259125
Homologene: 17491
Or9g19
Name: olfactory receptor family 9 subfamily G member 19
Synonyms: GA_x6K02T2Q125-47248551-47249468, MOR213-2, Olfr1013
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258757
Homologene: 74096
Mrpl40
Name: mitochondrial ribosomal protein L40
Synonyms: Nlvcf
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 18100
Homologene: 2800
Myg1
Name: melanocyte proliferating gene 1
Synonyms: Gamm1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 60315
Homologene: 5853
Gpr137c
Name: G protein-coupled receptor 137C
Synonyms: LOC380893, TM7SF1L2, 6330416L11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 70713
Homologene: 28613
Gstm6
Name: glutathione S-transferase, mu 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 14867
HGNC: HGNC:4632
Homologene: 117950
Zfp784
Name: zinc finger protein 784
Synonyms: C730049F21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 654801
Homologene: 52408
Ndufc1
Name: NADH:ubiquinone oxidoreductase subunit C1
Synonyms: 2310016K22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 66377
HGNC: HGNC:7705
Homologene: 1865
Gm16548
Name: predicted gene 16548
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 100503816
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 63,305,615 bp
  • C to T, chromosome 1 at 105,746,126 bp
  • C to T, chromosome 1 at 132,360,831 bp
  • T to C, chromosome 1 at 132,595,654 bp
  • C to T, chromosome 1 at 158,612,346 bp
  • G to C, chromosome 1 at 164,151,844 bp
  • T to C, chromosome 2 at 13,476,150 bp
  • T to A, chromosome 2 at 32,216,055 bp
  • T to C, chromosome 2 at 76,814,173 bp
  • A to T, chromosome 2 at 85,769,978 bp
  • G to A, chromosome 2 at 112,156,442 bp
  • A to T, chromosome 2 at 126,912,695 bp
  • G to A, chromosome 3 at 36,090,923 bp
  • A to C, chromosome 3 at 51,408,143 bp
  • T to A, chromosome 3 at 107,943,606 bp
  • T to A, chromosome 4 at 115,797,952 bp
  • T to A, chromosome 4 at 120,098,508 bp
  • G to A, chromosome 5 at 121,167,834 bp
  • G to C, chromosome 7 at 5,038,358 bp
  • G to A, chromosome 7 at 104,068,468 bp
  • A to T, chromosome 9 at 79,602,251 bp
  • A to T, chromosome 9 at 109,114,814 bp
  • A to G, chromosome 10 at 87,864,821 bp
  • T to C, chromosome 10 at 129,722,783 bp
  • A to G, chromosome 10 at 130,362,075 bp
  • G to A, chromosome 11 at 69,027,701 bp
  • T to A, chromosome 13 at 95,697,077 bp
  • A to G, chromosome 14 at 45,278,963 bp
  • G to A, chromosome 14 at 70,613,403 bp
  • T to A, chromosome 14 at 74,807,545 bp
  • C to A, chromosome 15 at 85,817,572 bp
  • G to C, chromosome 15 at 102,337,736 bp
  • A to T, chromosome 16 at 18,872,375 bp
  • A to G, chromosome 16 at 32,252,190 bp
  • A to C, chromosome 17 at 35,147,175 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2428 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040390-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.