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Strain Name:
C57BL/6J-MtgxR2483Btlr/Mmmh
Stock Number:
040407-MU
Citation ID:
RRID:MMRRC_040407-MU
Other Names:
R2483 (G1), C57BL/6J-MtgxR2483Btlr
Major Collection:

Strain Information

Btrc
Name: beta-transducin repeat containing protein
Synonyms: SCF b-TRCP, beta-TrCP, Slimb, Beta-Trcp1, Fbw1a
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 12234
VEGA: 19
HGNC: HGNC:1144
Homologene: 39330
Met
Name: met proto-oncogene
Synonyms: HGF receptor, c-Met, Par4
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 17295
HGNC: HGNC:7029
Homologene: 206
Myh7
Name: myosin, heavy polypeptide 7, cardiac muscle, beta
Synonyms: Myhc-b, Myhcb, beta-MHC, B-MHC, MYH-beta/slow, MyHC-I, betaMHC
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 140781
HGNC: HGNC:7577
Homologene: 68044
Cdkn2a
Name: cyclin dependent kinase inhibitor 2A
Synonyms: MTS1, p19ARF, p16INK4a, INK4a-ARF, p16, ARF-INK4a, Arf, Pctr1, p19ARF, Ink4a/Arf
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 12578
HGNC: HGNC:1787
Homologene: 55430
Htr1d
Name: 5-hydroxytryptamine (serotonin) receptor 1D
Synonyms: Gpcr14, Htr1db
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 15552
HGNC: HGNC:5289
Homologene: 20240
Slc17a5
Name: solute carrier family 17 (anion/sugar transporter), member 5
Synonyms: 4631416G20Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235504
Homologene: 56571
Emx1
Name: empty spiracles homeobox 1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 13796
HGNC: HGNC:3340
Homologene: 55799
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 37,444,696 bp
  • A to T, chromosome 1 at 72,262,075 bp
  • T to C, chromosome 1 at 92,855,938 bp
  • T to A, chromosome 1 at 130,103,072 bp
  • T to C, chromosome 1 at 135,969,537 bp
  • T to C, chromosome 1 at 156,431,099 bp
  • T to C, chromosome 2 at 30,186,698 bp
  • A to T, chromosome 2 at 67,524,992 bp
  • G to A, chromosome 2 at 89,483,127 bp
  • C to A, chromosome 2 at 94,364,569 bp
  • T to A, chromosome 2 at 132,381,175 bp
  • A to T, chromosome 2 at 178,374,595 bp
  • A to T, chromosome 2 at 178,466,589 bp
  • A to T, chromosome 2 at 180,260,429 bp
  • T to C, chromosome 3 at 109,341,166 bp
  • T to C, chromosome 3 at 114,112,032 bp
  • A to G, chromosome 4 at 89,276,630 bp
  • T to C, chromosome 4 at 136,443,504 bp
  • T to C, chromosome 4 at 153,965,519 bp
  • C to A, chromosome 5 at 3,976,235 bp
  • A to T, chromosome 5 at 34,465,758 bp
  • A to T, chromosome 5 at 76,856,409 bp
  • A to T, chromosome 5 at 87,520,893 bp
  • T to C, chromosome 5 at 110,719,236 bp
  • A to T, chromosome 5 at 112,858,408 bp
  • A to T, chromosome 6 at 17,549,086 bp
  • A to T, chromosome 6 at 24,765,738 bp
  • T to C, chromosome 6 at 47,897,941 bp
  • A to G, chromosome 6 at 56,736,843 bp
  • A to T, chromosome 6 at 58,435,452 bp
  • G to A, chromosome 6 at 83,194,187 bp
  • A to G, chromosome 6 at 83,763,262 bp
  • T to C, chromosome 6 at 85,188,255 bp
  • A to G, chromosome 6 at 85,332,278 bp
  • A to G, chromosome 6 at 123,816,038 bp
  • A to T, chromosome 6 at 126,844,399 bp
  • A to T, chromosome 6 at 132,868,470 bp
  • T to C, chromosome 7 at 92,859,592 bp
  • A to T, chromosome 7 at 100,998,499 bp
  • T to C, chromosome 7 at 104,579,942 bp
  • CAAATA to CA, chromosome 7 at 108,667,662 bp
  • G to A, chromosome 8 at 48,240,270 bp
  • G to A, chromosome 8 at 60,922,786 bp
  • G to C, chromosome 8 at 68,902,397 bp
  • A to G, chromosome 8 at 93,027,341 bp
  • A to G, chromosome 8 at 107,548,535 bp
  • A to T, chromosome 9 at 20,873,177 bp
  • A to G, chromosome 9 at 44,848,966 bp
  • A to T, chromosome 9 at 53,510,266 bp
  • G to A, chromosome 9 at 59,313,753 bp
  • T to A, chromosome 9 at 67,975,907 bp
  • A to T, chromosome 9 at 78,538,274 bp
  • A to T, chromosome 9 at 78,667,357 bp
  • C to A, chromosome 9 at 105,864,148 bp
  • T to C, chromosome 9 at 107,512,022 bp
  • A to G, chromosome 9 at 108,063,521 bp
  • T to G, chromosome 9 at 108,480,559 bp
  • A to G, chromosome 10 at 78,748,823 bp
  • A to G, chromosome 10 at 80,150,310 bp
  • A to G, chromosome 10 at 86,887,909 bp
  • A to G, chromosome 10 at 88,757,993 bp
  • A to G, chromosome 11 at 7,130,348 bp
  • T to C, chromosome 11 at 46,247,693 bp
  • A to G, chromosome 11 at 59,080,146 bp
  • A to T, chromosome 11 at 67,211,226 bp
  • G to A, chromosome 11 at 69,821,518 bp
  • T to C, chromosome 11 at 75,515,291 bp
  • C to T, chromosome 11 at 83,297,629 bp
  • G to A, chromosome 11 at 102,293,954 bp
  • A to G, chromosome 11 at 115,864,739 bp
  • A to G, chromosome 12 at 73,964,643 bp
  • A to T, chromosome 12 at 75,114,471 bp
  • A to T, chromosome 12 at 76,095,537 bp
  • T to C, chromosome 13 at 11,759,703 bp
  • T to G, chromosome 13 at 23,876,529 bp
  • C to A, chromosome 13 at 46,750,635 bp
  • A to G, chromosome 13 at 76,182,867 bp
  • G to A, chromosome 14 at 20,755,707 bp
  • G to A, chromosome 14 at 34,045,818 bp
  • C to G, chromosome 14 at 50,930,292 bp
  • T to C, chromosome 14 at 54,973,381 bp
  • A to G, chromosome 14 at 58,109,571 bp
  • A to T, chromosome 15 at 95,965,974 bp
  • A to G, chromosome 17 at 71,077,812 bp
  • T to A, chromosome 18 at 32,414,227 bp
  • T to A, chromosome 18 at 80,756,550 bp
  • T to C, chromosome 19 at 4,281,940 bp
  • T to A, chromosome 19 at 8,841,150 bp
  • T to A, chromosome 19 at 10,706,205 bp
  • T to C, chromosome 19 at 15,960,980 bp
  • A to G, chromosome 19 at 25,079,877 bp
  • A to G, chromosome 19 at 36,981,086 bp
  • A to G, chromosome 19 at 45,516,058 bp
  • A to T, chromosome 19 at 48,535,618 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2483 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040407-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.