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Strain Name:
C57BL/6J-MtgxR2518Btlr/Mmmh
Stock Number:
040422-MU
Citation ID:
RRID:MMRRC_040422-MU
Other Names:
R2518 (G1), C57BL/6J-MtgxR2518Btlr
Major Collection:

Strain Information

Col3a1
Name: collagen, type III, alpha 1
Synonyms: Col3a-1, Tsk-2, Tsk2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12825
HGNC: HGNC:2201
Homologene: 55433
Lrig3
Name: leucine-rich repeats and immunoglobulin-like domains 3
Synonyms: 9030421L11Rik, 9130004I02Rik, 9430095K15Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 320398
VEGA: 10
Homologene: 62452
Hdac5
Name: histone deacetylase 5
Synonyms: mHDA1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 15184
Homologene: 3995
Vps54
Name: VPS54 GARP complex subunit
Synonyms: Vps54l, 5330404P15Rik, mSLP8, wr
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 245944
Homologene: 5605
Tmem63b
Name: transmembrane protein 63b
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 224807
Homologene: 101682
Cyfip1
Name: cytoplasmic FMR1 interacting protein 1
Synonyms: P140SRA-1, Shyc, pl-1, l(7)1Rl, Sra-1, E030028J09Rik, l7Rl1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 20430
Homologene: 22628
Golga4
Name: golgin A4
Synonyms: golgin-245, Olp-1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 54214
HGNC: HGNC:4427
Homologene: 68224
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 44,129,615 bp
  • T to A, chromosome 1 at 45,337,512 bp
  • G to A, chromosome 1 at 98,446,828 bp
  • G to A, chromosome 1 at 133,360,124 bp
  • A to G, chromosome 1 at 171,380,208 bp
  • A to G, chromosome 1 at 172,119,901 bp
  • T to A, chromosome 2 at 52,249,511 bp
  • G to A, chromosome 2 at 127,428,291 bp
  • T to A, chromosome 2 at 132,750,527 bp
  • G to T, chromosome 2 at 156,073,971 bp
  • C to T, chromosome 3 at 5,403,358 bp
  • T to C, chromosome 3 at 66,978,359 bp
  • T to A, chromosome 4 at 101,612,930 bp
  • T to C, chromosome 4 at 112,025,481 bp
  • A to T, chromosome 4 at 114,599,903 bp
  • T to C, chromosome 4 at 129,720,747 bp
  • A to G, chromosome 4 at 139,302,499 bp
  • A to G, chromosome 4 at 143,417,903 bp
  • T to C, chromosome 5 at 21,305,528 bp
  • T to A, chromosome 5 at 38,227,932 bp
  • A to G, chromosome 5 at 115,987,046 bp
  • A to T, chromosome 5 at 129,699,035 bp
  • A to T, chromosome 6 at 14,719,378 bp
  • C to A, chromosome 6 at 85,667,963 bp
  • A to T, chromosome 6 at 131,630,073 bp
  • T to C, chromosome 7 at 30,576,068 bp
  • T to C, chromosome 7 at 44,220,737 bp
  • C to T, chromosome 7 at 55,928,284 bp
  • C to A, chromosome 7 at 81,317,422 bp
  • A to T, chromosome 7 at 86,616,187 bp
  • A to C, chromosome 8 at 4,266,588 bp
  • C to A, chromosome 8 at 11,005,352 bp
  • A to G, chromosome 8 at 24,637,141 bp
  • A to T, chromosome 9 at 44,948,137 bp
  • A to T, chromosome 9 at 97,079,071 bp
  • A to G, chromosome 9 at 118,556,612 bp
  • G to A, chromosome 10 at 7,907,481 bp
  • A to G, chromosome 10 at 53,073,534 bp
  • T to C, chromosome 10 at 125,994,441 bp
  • G to T, chromosome 11 at 20,725,797 bp
  • C to T, chromosome 11 at 21,306,394 bp
  • C to T, chromosome 11 at 53,480,336 bp
  • A to G, chromosome 11 at 102,197,136 bp
  • G to A, chromosome 11 at 105,348,431 bp
  • A to G, chromosome 12 at 11,457,067 bp
  • T to A, chromosome 12 at 102,222,051 bp
  • T to A, chromosome 12 at 103,455,824 bp
  • G to T, chromosome 13 at 26,769,749 bp
  • A to G, chromosome 13 at 67,347,939 bp
  • T to C, chromosome 13 at 90,062,188 bp
  • T to A, chromosome 13 at 104,413,819 bp
  • A to T, chromosome 13 at 114,881,042 bp
  • T to A, chromosome 14 at 41,122,512 bp
  • A to T, chromosome 14 at 60,232,660 bp
  • T to A, chromosome 15 at 58,450,600 bp
  • C to T, chromosome 15 at 71,463,911 bp
  • T to C, chromosome 15 at 78,405,833 bp
  • A to G, chromosome 15 at 102,518,557 bp
  • G to A, chromosome 16 at 97,922,086 bp
  • T to A, chromosome 17 at 29,681,980 bp
  • A to G, chromosome 17 at 45,666,154 bp
  • A to T, chromosome 17 at 46,260,095 bp
  • A to G, chromosome 18 at 89,207,327 bp
  • A to G, chromosome 19 at 13,862,309 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2518 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040422-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.