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Strain Name:
C57BL/6J-MtgxR2870Btlr/Mmmh
Stock Number:
040458-MU
Citation ID:
RRID:MMRRC_040458-MU
Other Names:
R2870 (G1), C57BL/6J-MtgxR2870Btlr
Major Collection:

Strain Information

Matr3
Name: matrin 3
Synonyms: D030046F20Rik, 2810017I02Rik, 1110061A14Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 17184
HGNC: HGNC:6912
Homologene: 7830
Gria2
Name: glutamate receptor, ionotropic, AMPA2 (alpha 2)
Synonyms: GluR-B, GluR2, Glur-2, Glur2, GluA2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 14800
HGNC: HGNC:4572
Homologene: 20225
Pzp
Name: PZP, alpha-2-macroglobulin like
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 11287
Homologene: 104112
Plin2
Name: perilipin 2
Synonyms: Adrp, adipophilin, ADPH, Adfp
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11520
HGNC: HGNC:248
Homologene: 872
Clasrp
Name: CLK4-associating serine/arginine rich protein
Synonyms: SWAP2, Srsf16, Sfrs16
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 53609
Homologene: 134306
Arhgef10
Name: Rho guanine nucleotide exchange factor 10
Synonyms: 6430549H08Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234094
Homologene: 22827
Dennd2b
Name: DENN domain containing 2B
Synonyms: 2610305K15Rik, 2010004M01Rik, St5, Denn2b
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 76954
Homologene: 3951
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 39,405,317 bp
  • A to T, chromosome 1 at 55,697,150 bp
  • A to G, chromosome 1 at 59,211,137 bp
  • C to T, chromosome 1 at 63,061,039 bp
  • A to G, chromosome 1 at 63,164,723 bp
  • GCCC to GC, chromosome 1 at 88,232,257 bp
  • GT to GTT, chromosome 1 at 88,266,524 bp
  • T to A, chromosome 1 at 93,357,863 bp
  • T to C, chromosome 2 at 86,553,584 bp
  • C to G, chromosome 2 at 118,119,378 bp
  • A to G, chromosome 2 at 152,919,348 bp
  • A to G, chromosome 3 at 30,599,698 bp
  • G to A, chromosome 3 at 80,702,492 bp
  • T to C, chromosome 3 at 116,923,751 bp
  • T to C, chromosome 3 at 130,703,508 bp
  • T to A, chromosome 3 at 135,886,793 bp
  • A to C, chromosome 4 at 59,093,850 bp
  • A to G, chromosome 4 at 63,550,021 bp
  • A to G, chromosome 4 at 86,668,678 bp
  • C to A, chromosome 4 at 115,487,301 bp
  • T to A, chromosome 4 at 148,540,030 bp
  • T to A, chromosome 4 at 152,107,503 bp
  • T to A, chromosome 5 at 8,180,863 bp
  • G to A, chromosome 5 at 16,312,568 bp
  • C to T, chromosome 5 at 22,049,791 bp
  • C to T, chromosome 5 at 31,257,861 bp
  • T to A, chromosome 5 at 90,569,595 bp
  • A to G, chromosome 5 at 104,212,108 bp
  • C to T, chromosome 5 at 105,331,000 bp
  • A to G, chromosome 5 at 123,452,667 bp
  • A to G, chromosome 5 at 125,638,268 bp
  • C to T, chromosome 6 at 119,214,774 bp
  • A to T, chromosome 6 at 128,485,556 bp
  • G to A, chromosome 6 at 131,370,413 bp
  • G to A, chromosome 6 at 136,765,593 bp
  • C to A, chromosome 7 at 19,585,240 bp
  • C to A, chromosome 7 at 46,046,937 bp
  • A to G, chromosome 7 at 64,363,190 bp
  • A to C, chromosome 7 at 85,233,626 bp
  • T to C, chromosome 7 at 87,602,722 bp
  • T to C, chromosome 7 at 99,839,761 bp
  • A to T, chromosome 7 at 109,557,430 bp
  • T to C, chromosome 8 at 14,975,093 bp
  • A to G, chromosome 8 at 14,975,666 bp
  • T to A, chromosome 8 at 69,816,367 bp
  • T to A, chromosome 8 at 79,661,073 bp
  • T to A, chromosome 9 at 4,503,614 bp
  • A to C, chromosome 9 at 64,304,327 bp
  • G to A, chromosome 10 at 4,997,890 bp
  • C to T, chromosome 10 at 10,431,281 bp
  • A to T, chromosome 10 at 22,067,379 bp
  • C to T, chromosome 10 at 41,966,700 bp
  • G to A, chromosome 10 at 79,645,653 bp
  • GCCC to GCCCC, chromosome 10 at 81,178,767 bp
  • A to G, chromosome 10 at 88,904,963 bp
  • A to T, chromosome 10 at 105,841,527 bp
  • A to G, chromosome 10 at 118,150,942 bp
  • A to T, chromosome 10 at 123,704,365 bp
  • A to T, chromosome 10 at 129,669,759 bp
  • C to T, chromosome 11 at 3,242,586 bp
  • C to A, chromosome 11 at 3,976,834 bp
  • T to C, chromosome 11 at 23,761,129 bp
  • A to G, chromosome 11 at 70,724,081 bp
  • A to G, chromosome 11 at 78,076,278 bp
  • T to G, chromosome 11 at 100,047,873 bp
  • T to G, chromosome 14 at 52,410,861 bp
  • A to G, chromosome 14 at 56,386,950 bp
  • T to A, chromosome 15 at 7,250,179 bp
  • C to T, chromosome 15 at 47,857,924 bp
  • A to G, chromosome 16 at 48,843,612 bp
  • A to T, chromosome 17 at 21,508,078 bp
  • T to C, chromosome 17 at 34,200,170 bp
  • G to A, chromosome 17 at 35,021,242 bp
  • G to T, chromosome 17 at 35,029,181 bp
  • T to C, chromosome 17 at 57,053,847 bp
  • T to A, chromosome 17 at 69,418,111 bp
  • A to T, chromosome 17 at 78,932,103 bp
  • A to T, chromosome 17 at 80,251,376 bp
  • T to A, chromosome 18 at 35,572,296 bp
  • A to T, chromosome 18 at 37,505,780 bp
  • A to G, chromosome 19 at 10,794,626 bp
  • A to T, chromosome 19 at 11,789,574 bp
  • T to C, chromosome X at 71,296,362 bp
  • T to A, chromosome X at 74,339,396 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2870 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040458-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.