Strain Name:
C57BL/6J-MtgxR2889Btlr/Mmmh
Stock Number:
040477-MU
Citation ID:
RRID:MMRRC_040477-MU
Other Names:
R2889 (G1), C57BL/6J-MtgxR2889Btlr
Major Collection:

Gene Information

Adamts20
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 20
Synonyms: ADAMTS-20, bt
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 223838
Homologene: 11808
Msantd3
Name: Myb/SANT-like DNA-binding domain containing 3
Synonyms: 2410046L14Rik, 2810429P03Rik, 5730528L13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 66665
Homologene: 12452
Aurka
Name: aurora kinase A
Synonyms: Ayk1, Ark1, IAK, AIRK1, aurora A, Aurora-A, Stk6, IAK1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 20878
Homologene: 2670
Rsrc2
Name: arginine/serine-rich coiled-coil 2
Synonyms: 1500011J06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 208606
Homologene: 133892
Son
Name: Son DNA binding protein
Synonyms: 2900011L12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 20658
Homologene: 10551
Ecpas
Name: Ecm29 proteasome adaptor and scaffold
Synonyms: AI314180
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230249
Homologene: 6056
Zfy1
Name: zinc finger protein 1, Y-linked
Synonyms: Zfy-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: Y
NCBI: 22767
Homologene: 56456
Zfhx2
Name: zinc finger homeobox 2
Synonyms: zfh-5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 239102
Homologene: 52657
Ryr1
Name: ryanodine receptor 1, skeletal muscle
Synonyms: calcium release channel isoform 1, Ryr, skrr
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 20190
Homologene: 68069
Pclo
Name: piccolo (presynaptic cytomatrix protein)
Synonyms: Acz, Pico
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 26875
Homologene: 69111
Ppp1r3a
Name: protein phosphatase 1, regulatory subunit 3A
Synonyms: GM, RGL
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 140491
HGNC: HGNC:9291
Homologene: 48124
Vmn2r103
Name: vomeronasal 2, receptor 103
Synonyms: EG627636
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 627636
Homologene: 115024
Wdfy4
Name: WD repeat and FYVE domain containing 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 545030
Homologene: 83473
Sptbn5
Name: spectrin beta, non-erythrocytic 5
Synonyms: EG640524, Spnb5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 640524
Homologene: 41150
Pmfbp1
Name: polyamine modulated factor 1 binding protein 1
Synonyms: F77, 1700016D22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 56523
Homologene: 23182
Npr2
Name: natriuretic peptide receptor 2
Synonyms: guanylyl cyclase-B, cn, pwe
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230103
HGNC: HGNC:7944
Homologene: 2970
Spef2
Name: sperm flagellar 2
Synonyms: C230086A09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 320277
Homologene: 23371
Lamp1
Name: lysosomal-associated membrane protein 1
Synonyms: CD107a, Lamp-1, Perk
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 16783
HGNC: HGNC:6499
Homologene: 4061
Vmn2r13
Name: vomeronasal 2, receptor 13
Synonyms: Gm4867
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231589
Homologene: 129606
Pcdhb9
Name: protocadherin beta 9
Synonyms: Pcdhb4C, PcdhbI
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 93880
HGNC: HGNC:8689
Homologene: 87124
Adam28
Name: a disintegrin and metallopeptidase domain 28
Synonyms: Dtgn1, MDC-L, D430033C21Rik, C130072N01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 13522
VEGA: 14
HGNC: HGNC:206
Homologene: 40705
Phex
Name: phosphate regulating endopeptidase homolog, X-linked
Synonyms: HPDR1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 18675
HGNC: HGNC:8918
Homologene: 37310
Ankrd6
Name: ankyrin repeat domain 6
Synonyms: diversin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 140577
Homologene: 8967
Or5p56
Name: olfactory receptor family 5 subfamily P member 56
Synonyms: GA_x6K02T2PBJ9-10319672-10320604, MOR204-1, Olfr477
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258928
Homologene: 27246
Kcnq5
Name: potassium voltage-gated channel, subfamily Q, member 5
Synonyms: 9230107O05Rik, D1Mgi1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226922
HGNC: HGNC:6299
Homologene: 28270
Or6z3
Name: olfactory receptor family 6 subfamily Z member 3
Synonyms: GA_x6K02T2QGBW-3190370-3191314, MOR103-7, Olfr1336
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258917
Homologene: 37007
Slc35a5
Name: solute carrier family 35, member A5
Synonyms: D16Ertd450e, 1010001J06Rik, D730043G07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 74102
Homologene: 9930
Zfp189
Name: zinc finger protein 189
Synonyms: C430015I23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230162
Homologene: 20737
4930480E11Rik
Name: RIKEN cDNA 4930480E11 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 74910
Homologene: 136363
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 21,402,302 bp
  • A to G, chromosome 2 at 120,064,076 bp
  • A to G, chromosome 2 at 172,367,120 bp
  • C to A, chromosome 4 at 32,818,704 bp
  • T to C, chromosome 4 at 43,641,600 bp
  • T to C, chromosome 4 at 48,552,494 bp
  • A to G, chromosome 4 at 49,521,547 bp
  • T to C, chromosome 4 at 58,836,165 bp
  • C to A, chromosome 5 at 14,856,981 bp
  • T to C, chromosome 5 at 109,191,974 bp
  • A to T, chromosome 5 at 123,736,557 bp
  • T to G, chromosome 6 at 14,718,249 bp
  • T to A, chromosome 7 at 6,460,941 bp
  • T to C, chromosome 7 at 29,078,741 bp
  • A to T, chromosome 7 at 107,990,577 bp
  • T to A, chromosome 8 at 13,173,891 bp
  • T to C, chromosome 8 at 109,525,431 bp
  • C to A, chromosome 14 at 33,109,519 bp
  • T to C, chromosome 14 at 55,064,803 bp
  • A to T, chromosome 14 at 68,634,845 bp
  • G to A, chromosome 15 at 9,630,613 bp
  • C to T, chromosome 15 at 94,330,578 bp
  • A to G, chromosome 16 at 45,151,560 bp
  • T to C, chromosome 16 at 91,659,899 bp
  • T to C, chromosome 17 at 19,793,600 bp
  • G to T, chromosome 18 at 37,403,223 bp
  • A to T, chromosome X at 78,370,682 bp
  • T to C, chromosome X at 157,310,958 bp
  • G to A, chromosome Y at 726,307 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2889 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040477-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.