Strain Name:
C57BL/6J-MtgxR2899Btlr/Mmmh
Stock Number:
040487-MU
Citation ID:
RRID:MMRRC_040487-MU
Other Names:
R2899 (G1), C57BL/6J-MtgxR2899Btlr
Major Collection:

Gene Information

Zfp143
Name: zinc finger protein 143
Synonyms: D7Ertd805e, pHZ-1, Staf, Zfp80-rs1, KRAB14, Zfp79
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 20841
Homologene: 56444
Nf1
Name: neurofibromin 1
Synonyms: neurofibromin, Nf-1, Mhdadsk9, Dsk9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 18015
HGNC: HGNC:7765
Homologene: 226
Cep131
Name: centrosomal protein 131
Synonyms: AZ1, Azi1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 12009
Homologene: 7638
Exoc2
Name: exocyst complex component 2
Synonyms: Sec5l1, 2410030I24Rik, Sec5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 66482
Homologene: 10122
Rassf1
Name: Ras association (RalGDS/AF-6) domain family member 1
Synonyms: Rassf1C, RDA32, NORE2A, Rassf1A, REH3P21, Rassf1B, 123F protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 56289
HGNC: HGNC:9882
Homologene: 10499
Clmp
Name: CXADR-like membrane protein
Synonyms: 9030425E11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 71566
Homologene: 11705
Limk1
Name: LIM-domain containing, protein kinase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 16885
HGNC: HGNC:6613
Homologene: 1738
Syngap1
Name: synaptic Ras GTPase activating protein 1 homolog (rat)
Synonyms: Syngap
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 240057
Homologene: 84739
Pask
Name: PAS domain containing serine/threonine kinase
Synonyms: Paskin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 269224
Homologene: 9038
Tsku
Name: tsukushi, small leucine rich proteoglycan
Synonyms: 9530051K01Rik, Lrrc54
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 244152
Homologene: 41045
Rdx
Name: radixin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 19684
VEGA: 9
HGNC: HGNC:9944
Homologene: 37707
Epha5
Name: Eph receptor A5
Synonyms: Hek7, Rek7, Cek7, bsk, Els1, Ehk1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 13839
HGNC: HGNC:3389
Homologene: 55824
Lrrc37a
Name: leucine rich repeat containing 37A
Synonyms: LOC237954
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 237954
Homologene: 138824
F5
Name: coagulation factor V
Synonyms: Cf5, Cf-5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 14067
HGNC: HGNC:3542
Homologene: 104
1110002E22Rik
Name: RIKEN cDNA 1110002E22 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 102634333
Homologene: 131709
Zkscan3
Name: zinc finger with KRAB and SCAN domains 3
Synonyms: Skz1, 2810435N07Rik, Zfp307, Zfp306
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 72739
Homologene: 130732
Amigo3
Name: adhesion molecule with Ig like domain 3
Synonyms: E430002N15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 320844
Homologene: 45570
Olfr1453
Name: olfactory receptor 1453
Synonyms: GA_x6K02T2RE5P-3357666-3356743, MOR202-6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 258695
Homologene: 120159
Zc3h6
Name: zinc finger CCCH type containing 6
Synonyms: 4833425H18Rik, 4631426G04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 78751
Homologene: 35328
Usp36
Name: ubiquitin specific peptidase 36
Synonyms: 2700002L06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 72344
Homologene: 11828
FP885536.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
4930430A15Rik
Name: RIKEN cDNA 4930430A15 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 67575
Homologene: 69410
Fuca1
Name: fucosidase, alpha-L- 1, tissue
Synonyms: Afuc, 0610006A03Rik, 9530055J05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 71665
HGNC: HGNC:4006
Homologene: 20078
Gdf7
Name: growth differentiation factor 7
Synonyms: BMP12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 238057
VEGA: 12
HGNC: HGNC:4222
Homologene: 32177
Bahd1
Name: bromo adjacent homology domain containing 1
Synonyms: LOC228536
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 228536
Homologene: 8976
Dusp6
Name: dual specificity phosphatase 6
Synonyms: MKP-3, MKP3, PYST1, 1300019I03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 67603
VEGA: 10
HGNC: HGNC:3072
Homologene: 55621
Cd200r4
Name: CD200 receptor 4
Synonyms: F630107N04Rik, MCD200RLa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 239849
Homologene: 45557
Saraf
Name: store-operated calcium entry-associated regulatory factor
Synonyms: 1810045K07Rik, Tmem66
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 67887
Homologene: 9398
Fbxo3
Name: F-box protein 3
Synonyms: 1700026K02Rik, Fba, 1200002G09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 57443
Homologene: 8133
Pou4f3
Name: POU domain, class 4, transcription factor 3
Synonyms: Brn3c, Brn3.1, Brn-3.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 18998
HGNC: HGNC:9220
Homologene: 2023
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 93,334,547 bp
  • A to G, chromosome 1 at 164,186,900 bp
  • T to A, chromosome 2 at 104,051,135 bp
  • C to A, chromosome 2 at 111,220,670 bp
  • C to T, chromosome 2 at 118,916,406 bp
  • T to C, chromosome 2 at 129,002,232 bp
  • A to G, chromosome 3 at 138,065,682 bp
  • G to A, chromosome 4 at 135,923,012 bp
  • G to T, chromosome 4 at 156,334,397 bp
  • T to A, chromosome 5 at 84,233,808 bp
  • A to T, chromosome 5 at 134,688,300 bp
  • T to C, chromosome 7 at 98,352,917 bp
  • T to A, chromosome 7 at 110,072,129 bp
  • T to C, chromosome 8 at 34,161,231 bp
  • T to C, chromosome 9 at 40,782,392 bp
  • T to C, chromosome 9 at 52,068,911 bp
  • G to A, chromosome 9 at 107,554,194 bp
  • T to C, chromosome 9 at 108,054,154 bp
  • T to C, chromosome 10 at 99,263,845 bp
  • T to G, chromosome 11 at 79,412,758 bp
  • G to A, chromosome 11 at 103,497,864 bp
  • G to A, chromosome 11 at 118,276,756 bp
  • T to C, chromosome 11 at 120,072,028 bp
  • C to T, chromosome 12 at 8,298,470 bp
  • A to T, chromosome 13 at 21,393,973 bp
  • A to G, chromosome 13 at 30,905,769 bp
  • T to C, chromosome 16 at 44,833,365 bp
  • A to G, chromosome 17 at 26,959,985 bp
  • T to C, chromosome 18 at 42,395,523 bp
  • T to A, chromosome 19 at 13,027,694 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2899 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040487-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.