Strain Name:
C57BL/6J-MtgxR2994Btlr/Mmmh
Stock Number:
040529-MU
Citation ID:
RRID:MMRRC_040529-MU
Other Names:
R2994 (G1), C57BL/6J-MtgxR2994Btlr
Major Collection:

Strain Information

Nedd4
Name: neural precursor cell expressed, developmentally down-regulated 4
Synonyms: E430025J12Rik, Nedd4-1, Nedd4, Nedd4a
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17999
HGNC: HGNC:7727
Homologene: 134533
Tjp2
Name: tight junction protein 2
Synonyms: ZO-2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 21873
VEGA: 19
Homologene: 3541
Khdc4
Name: KH domain containing 4, pre-mRNA splicing factor
Synonyms: 2810403A07Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 74200
Homologene: 41761
Synj2
Name: synaptojanin 2
Synonyms: SJ2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 20975
Homologene: 117703
Agrn
Name: agrin
Synonyms: nmf380, NMF380, Agrin
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11603
HGNC: HGNC:329
Homologene: 27907
Tg
Name: thyroglobulin
Synonyms: Tgn
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 21819
Homologene: 2430
Gpsm1
Name: G-protein signalling modulator 1 (AGS3-like, C. elegans)
Synonyms: Ags3, 1810037C22Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 67839
Homologene: 16987
Ripor2
Name: RHO family interacting cell polarization regulator 2
Synonyms: Fam65b, 6330500D04Rik, 1700108N18Rik, E430013J17Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 193385
Homologene: 9284
Psmd11
Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 11
Synonyms: 2610024G20Rik, 1700089D09Rik, 2810055C24Rik, C78232, 1810019E17Rik, S9, P44.5
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 69077
HGNC: HGNC:9556
Homologene: 2108
Zfp459
Name: zinc finger protein 459
Synonyms: 9930025G17Rik, Rslcan-14
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 328274
Abca3
Name: ATP-binding cassette, sub-family A member 3
Synonyms: ABC-C, Abc3, 1810036E22Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 27410
HGNC: HGNC:33
Homologene: 37437
Rundc3a
Name: RUN domain containing 3A
Synonyms: Rpip8, Rap2ip
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 51799
Homologene: 4871
Nlgn1
Name: neuroligin 1
Synonyms: 6330415N05Rik, NL1, Nlg1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 192167
Homologene: 56690
Arsi
Name: arylsulfatase i
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 545260
Homologene: 44428
Sh3rf1
Name: SH3 domain containing ring finger 1
Synonyms: Sh3md2, 2200003J05Rik, Posh
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 59009
Homologene: 10988
Or5d18
Name: olfactory receptor family 5 subfamily D member 18
Synonyms: MOR174-9, Olfr73, mOR-EG, GA_x6K02T2Q125-49527073-49526132
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 117004
Homologene: 133889
Btnl6
Name: butyrophilin-like 6
Synonyms: NG13, Gm6519
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 624681
Homologene: 106849
Or1e35
Name: olfactory receptor family 1 subfamily E member 35
Synonyms: MOR135-10, GA_x6K02T2P1NL-4062605-4061667, Olfr395
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 259007
Zfp629
Name: zinc finger protein 629
Synonyms: 9330199A09Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 320683
Homologene: 65318
Arhgef15
Name: Rho guanine nucleotide exchange factor 15
Synonyms: D530030K12Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 442801
Homologene: 18345
Slc13a2
Name: solute carrier family 13 (sodium-dependent dicarboxylate transporter), member 2
Synonyms: mNaDC-1, sodium/dicarboxylate co-transporter, Nadc1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20500
Homologene: 2965
Atp13a4
Name: ATPase type 13A4
Synonyms: 9330174J19Rik, 4631413J11Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 224079
Homologene: 75330
Cfap52
Name: cilia and flagella associated protein 52
Synonyms: 1700019F09Rik, Wdr16, 4933417B11Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 71860
Homologene: 12416
CK137956
Name: cDNA sequence CK137956
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 635169
Homologene: 57187
Gbp4
Name: guanylate binding protein 4
Synonyms: Mpa-2, Mpa2, Mag-2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 17472
Homologene: 128731
Polr1e
Name: polymerase (RNA) I polypeptide E
Synonyms: Praf1, 53kDa, D030019D19Rik, Paf53
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 64424
Homologene: 41486
Oprk1
Name: opioid receptor, kappa 1
Synonyms: KOR-1, Oprk2, R21
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18387
HGNC: HGNC:8154
Homologene: 20253
Fpr3
Name: formyl peptide receptor 3
Synonyms: Lxa4r, Fprl1, Fpr-rs1, LXA4-R
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 14294
Slc22a17
Name: solute carrier family 22 (organic cation transporter), member 17
Synonyms: 1700094C23Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 59049
VEGA: 14
Homologene: 10684
Zfp612
Name: zinc finger protein 612
Synonyms: B230354B21Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234725
Homologene: 27083
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 5,602,732 bp
  • G to A, chromosome 2 at 26,319,831 bp
  • A to G, chromosome 2 at 88,034,957 bp
  • C to T, chromosome 3 at 25,435,998 bp
  • A to G, chromosome 3 at 88,693,136 bp
  • T to C, chromosome 4 at 45,027,473 bp
  • T to C, chromosome 4 at 127,951,507 bp
  • T to C, chromosome 4 at 156,167,328 bp
  • A to T, chromosome 5 at 105,137,020 bp
  • T to C, chromosome 7 at 127,611,056 bp
  • A to G, chromosome 8 at 61,372,575 bp
  • A to G, chromosome 8 at 110,089,417 bp
  • A to G, chromosome 9 at 72,730,903 bp
  • T to A, chromosome 11 at 67,939,791 bp
  • T to C, chromosome 11 at 68,946,359 bp
  • T to C, chromosome 11 at 73,906,715 bp
  • T to A, chromosome 11 at 78,404,737 bp
  • T to C, chromosome 11 at 80,460,667 bp
  • C to T, chromosome 11 at 102,400,663 bp
  • A to T, chromosome 13 at 24,701,627 bp
  • G to A, chromosome 13 at 67,408,734 bp
  • T to C, chromosome 14 at 54,909,834 bp
  • A to T, chromosome 15 at 66,681,953 bp
  • A to T, chromosome 16 at 29,407,528 bp
  • C to A, chromosome 17 at 6,016,253 bp
  • C to T, chromosome 17 at 17,970,868 bp
  • T to C, chromosome 17 at 24,384,564 bp
  • C to A, chromosome 17 at 34,515,524 bp
  • G to A, chromosome 18 at 60,916,651 bp
  • T to C, chromosome 19 at 24,112,851 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2994 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040529-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.