Strain Name:
C57BL/6J-MtgxR3001Btlr/Mmmh
Stock Number:
040530-MU
Citation ID:
RRID:MMRRC_040530-MU
Other Names:
R3001 (G1), C57BL/6J-MtgxR3001Btlr
Major Collection:

Gene Information

Casq2
Name: calsequestrin 2
Synonyms: cardiac calsequestrin, ESTM52, cCSQ, Csq2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 12373
HGNC: HGNC:1513
Homologene: 20330
Rptor
Name: regulatory associated protein of MTOR, complex 1
Synonyms: raptor, 4932417H02Rik, Rap
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 74370
Homologene: 80210
Lyst
Name: lysosomal trafficking regulator
Synonyms: D13Sfk13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 17101
VEGA: 13
HGNC: HGNC:1968
Homologene: 61
Eprs
Name: glutamyl-prolyl-tRNA synthetase
Synonyms: 2410081F06Rik, 3010002K18Rik, Qprs
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 107508
HGNC: HGNC:3418
Homologene: 5870
Acly
Name: ATP citrate lyase
Synonyms: A730098H14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 104112
HGNC: HGNC:115
Homologene: 854
Sacm1l
Name: SAC1 suppressor of actin mutations 1-like (yeast)
Synonyms: Sac1p, SAC1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 83493
VEGA: 9
Homologene: 6320
Flnb
Name: filamin, beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 286940
HGNC: HGNC:3755
Homologene: 37480
Fbxl17
Name: F-box and leucine-rich repeat protein 17
Synonyms: Fbx13, C130023C01Rik, 6330576B01Rik, Fbxo13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 50758
Homologene: 79859
Tut4
Name: terminal uridylyl transferase 4
Synonyms: 6030404K05Rik, 9230115F04Rik, Tent3a, Zcchc11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230594
Homologene: 35279
Bnip3
Name: BCL2/adenovirus E1B interacting protein 3
Synonyms: Nip3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 12176
HGNC: HGNC:1084
Homologene: 2990
Polr1a
Name: polymerase (RNA) I polypeptide A
Synonyms: mRPA1, RPA194, 194kDa, 3010014K16Rik, Rpo1-4, 2900087K15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 20019
Homologene: 7033
Fasn
Name: fatty acid synthase
Synonyms: FAS, A630082H08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 14104
HGNC: HGNC:3594
Homologene: 55800
Xpo7
Name: exportin 7
Synonyms: Ranbp16, 4930506C02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 65246
VEGA: 14
Homologene: 22857
Orc3
Name: origin recognition complex, subunit 3
Synonyms: Orc3l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 50793
HGNC: HGNC:8489
Homologene: 8225
Slitrk5
Name: SLIT and NTRK-like family, member 5
Synonyms: 2610019D03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 75409
VEGA: 14
Homologene: 9193
Lrig1
Name: leucine-rich repeats and immunoglobulin-like domains 1
Synonyms: LIG-1, Img
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 16206
Homologene: 7380
Pon2
Name: paraoxonase 2
Synonyms: 6330405I24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 330260
HGNC: HGNC:9205
Homologene: 385
Usp33
Name: ubiquitin specific peptidase 33
Synonyms: Vdu1, 9830169D19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 170822
Homologene: 8996
Csmd1
Name: CUB and Sushi multiple domains 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 94109
Homologene: 69536
Lct
Name: lactase
Synonyms: LOC226413, Lphl, LPH
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226413
HGNC: HGNC:6530
Homologene: 124204
Zkscan17
Name: zinc finger with KRAB and SCAN domains 17
Synonyms: Nizp1, Zfp496
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 268417
Homologene: 13108
Phf11c
Name: PHD finger protein 11C
Synonyms: Gm6907
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 628705
Homologene: 87807
A2m
Name: alpha-2-macroglobulin
Synonyms: A2mp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232345
HGNC: HGNC:7
Homologene: 37248
Krt71
Name: keratin 71
Synonyms: mK6irs, Cu, mK6irs1, Ca, Krt2-6g, Cal4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 56735
Homologene: 88864
Ccdc180
Name: coiled-coil domain containing 180
Synonyms: LOC381522, E230008N13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 381522
Homologene: 117988
Lnx2
Name: ligand of numb-protein X 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 140887
Homologene: 17737
Tdrd1
Name: tudor domain containing 1
Synonyms: MTR-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 83561
Homologene: 12850
Dnai2
Name: dynein axonemal intermediate chain 2
Synonyms: C030015H18Rik, Dnaic2, b2b3405Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 432611
Homologene: 11311
Vmn2r98
Name: vomeronasal 2, receptor 98
Synonyms: EG224552
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224552
Homologene: 115024
Il27ra
Name: interleukin 27 receptor, alpha
Synonyms: WSX-1, IL-27R, Tccr
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 50931
Homologene: 3562
Igsf11
Name: immunoglobulin superfamily, member 11
Synonyms: BT-IgSF, 1700025L02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 207683
Homologene: 17613
Plxnc1
Name: plexin C1
Synonyms: vespr, CD232, 2510048K12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 54712
HGNC: HGNC:9106
Homologene: 4211
Ndufa8
Name: NADH:ubiquinone oxidoreductase subunit A8
Synonyms: 0610033L03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 68375
HGNC: HGNC:7692
Homologene: 40932
Tmtc4
Name: transmembrane and tetratricopeptide repeat containing 4
Synonyms: 5730419O14Rik, 4930403J22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 70551
Homologene: 32796
Otulinl
Name: OTU deubiquitinase with linear linkage specificity like
Synonyms: Fam105a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 223433
VEGA: 15
Homologene: 41302
Mindy4
Name: MINDY lysine 48 deubiquitinase 4
Synonyms: LOC384387, C330043M08Rik, Fam188b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 330323
Homologene: 49991
Sele
Name: selectin, endothelial cell
Synonyms: E-selectin, CD62E, Elam
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 20339
Homologene: 389
Klhdc1
Name: kelch domain containing 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 271005
Homologene: 18155
Gm4846
Name: predicted gene 4846
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226601
Homologene: 136281
Acd
Name: adrenocortical dysplasia
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 497652
Homologene: 23391
Pkd2l1
Name: polycystic kidney disease 2-like 1
Synonyms: polycystin-L, Pkdl, PCL, TRPP3, PKD2L
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 329064
HGNC: HGNC:9011
Homologene: 22946
Cep112
Name: centrosomal protein 112
Synonyms: 1700001M19Rik, 1700029K01Rik, 8430407H02Rik, Macoco, Ccdc46
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 76380
Homologene: 44915
Tmem181a
Name: transmembrane protein 181A
Synonyms: 5930418K15Rik, Gpr178, C76977, Tmem181
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 77106
Homologene: 44787
Wfdc6a
Name: WAP four-disulfide core domain 6A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 209351
Homologene: 128601
2810459M11Rik
Name: RIKEN cDNA 2810459M11 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 72792
Homologene: 54780
Ptcd1
Name: pentatricopeptide repeat domain 1
Synonyms: 1110069M14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 71799
Homologene: 33161
Otub2
Name: OTU domain, ubiquitin aldehyde binding 2
Synonyms: 2010015L18Rik, OTU2, OTB2, 4930586I02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 68149
Homologene: 57056
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 86,046,080 bp
  • T to C, chromosome 1 at 128,304,226 bp
  • G to T, chromosome 1 at 164,053,571 bp
  • T to C, chromosome 1 at 166,486,878 bp
  • T to C, chromosome 1 at 185,424,391 bp
  • T to A, chromosome 2 at 36,036,559 bp
  • C to T, chromosome 2 at 164,580,305 bp
  • A to G, chromosome 3 at 102,145,201 bp
  • A to T, chromosome 3 at 152,357,942 bp
  • T to C, chromosome 4 at 34,571,790 bp
  • A to G, chromosome 4 at 45,899,988 bp
  • G to A, chromosome 4 at 108,512,928 bp
  • G to A, chromosome 5 at 145,159,576 bp
  • A to G, chromosome 5 at 147,019,015 bp
  • A to G, chromosome 6 at 5,268,976 bp
  • T to A, chromosome 6 at 55,218,364 bp
  • T to A, chromosome 6 at 71,965,644 bp
  • G to A, chromosome 6 at 94,608,777 bp
  • A to G, chromosome 6 at 121,661,447 bp
  • T to C, chromosome 7 at 138,894,701 bp
  • A to G, chromosome 8 at 16,196,170 bp
  • G to T, chromosome 8 at 84,032,031 bp
  • C to T, chromosome 8 at 105,700,281 bp
  • T to C, chromosome 9 at 123,585,084 bp
  • A to T, chromosome 10 at 94,793,218 bp
  • A to G, chromosome 11 at 59,487,251 bp
  • T to C, chromosome 11 at 100,504,227 bp
  • A to G, chromosome 11 at 108,440,503 bp
  • C to T, chromosome 11 at 114,750,471 bp
  • G to A, chromosome 11 at 119,872,371 bp
  • T to C, chromosome 11 at 120,809,845 bp
  • T to A, chromosome 12 at 69,256,209 bp
  • T to C, chromosome 12 at 103,404,277 bp
  • A to T, chromosome 13 at 13,696,705 bp
  • G to T, chromosome 14 at 7,907,162 bp
  • A to G, chromosome 14 at 59,384,840 bp
  • A to G, chromosome 14 at 70,692,645 bp
  • A to G, chromosome 14 at 111,679,582 bp
  • A to T, chromosome 14 at 122,932,818 bp
  • T to C, chromosome 15 at 27,664,706 bp
  • C to T, chromosome 15 at 101,740,471 bp
  • T to C, chromosome 16 at 39,008,772 bp
  • T to A, chromosome 17 at 6,295,786 bp
  • A to G, chromosome 17 at 19,065,863 bp
  • T to A, chromosome 17 at 63,225,077 bp
  • A to G, chromosome 19 at 44,155,557 bp
  • C to A, chromosome 19 at 56,861,750 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3001 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040530-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.