Strain Name:
C57BL/6J-MtgxR3028Btlr/Mmmh
Stock Number:
040544-MU
Citation ID:
RRID:MMRRC_040544-MU
Other Names:
R3028 (G1), C57BL/6J-MtgxR3028Btlr
Major Collection:

Strain Information

Angpt2
Name: angiopoietin 2
Synonyms: Ang2, Ang-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 11601
HGNC: HGNC:485
Homologene: 22401
Hcrtr1
Name: hypocretin (orexin) receptor 1
Synonyms: OX1R
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230777
HGNC: HGNC:4848
Homologene: 37492
Klhdc4
Name: kelch domain containing 4
Synonyms: G430025P05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234825
Homologene: 69234
Rab11fip3
Name: RAB11 family interacting protein 3 (class II)
Synonyms: Rab11-FIP3, D030060O14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 215445
Homologene: 49396
Spc24
Name: SPC24, NDC80 kinetochore complex component, homolog (S. cerevisiae)
Synonyms: 2410030K01Rik, Spbc24
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 67629
VEGA: 9
Homologene: 12166
Hspg2
Name: perlecan (heparan sulfate proteoglycan 2)
Synonyms: per, Pcn, Plc, perlecan
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 15530
HGNC: HGNC:5273
Homologene: 68473
Ppib
Name: peptidylprolyl isomerase B
Synonyms: cyclophilin B, CyP-20b, Cphn-2, Cphn2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 19035
VEGA: 9
HGNC: HGNC:9255
Homologene: 726
Gtpbp1
Name: GTP binding protein 1
Synonyms: GP-1, GTPBP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 14904
VEGA: 15
HGNC: HGNC:4669
Homologene: 3165
Kirrel
Name: kirre like nephrin family adhesion molecule 1
Synonyms: Neph1, Kirrel1, 6720469N11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 170643
Homologene: 10089
Acsl3
Name: acyl-CoA synthetase long-chain family member 3
Synonyms: 2610510B12Rik, C85929, Facl3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 74205
HGNC: HGNC:3570
Homologene: 3278
Vrk3
Name: vaccinia related kinase 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 101568
Homologene: 9509
Susd6
Name: sushi domain containing 6
Synonyms: 4933426M11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 217684
VEGA: 12
Homologene: 40980
Sigirr
Name: single immunoglobulin and toll-interleukin 1 receptor (TIR) domain
Synonyms: Sigirr, Tir8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 24058
Homologene: 36399
Pdgfra
Name: platelet derived growth factor receptor, alpha polypeptide
Synonyms: CD140a, Pdgfr-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 18595
HGNC: HGNC:8803
Homologene: 31361
Avl9
Name: AVL9 cell migration associated
Synonyms: D730049P16Rik, 5830411G16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 78937
Homologene: 62425
Cyp4a10
Name: cytochrome P450, family 4, subfamily a, polypeptide 10
Synonyms: RP1, Cyp4a, D4Rp1, Msp-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 13117
Homologene: 128044
Skint6
Name: selection and upkeep of intraepithelial T cells 6
Synonyms: OTTMUSG00000008519
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230622
Homologene: 135888
Crybg2
Name: crystallin beta-gamma domain containing 2
Synonyms: Aim1l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230806
Homologene: 19232
Phlpp2
Name: PH domain and leucine rich repeat protein phosphatase 2
Synonyms: C130044A18Rik, Phlppl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244650
Homologene: 71015
Hipk3
Name: homeodomain interacting protein kinase 3
Synonyms: DYRK6, FIST3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 15259
HGNC: HGNC:4915
Homologene: 55923
Sftpc
Name: surfactant associated protein C
Synonyms: SP-C, Sftp-2, Sftp2, pro-SpC, Bricd6, SPC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 20389
VEGA: 14
Homologene: 2271
AC135640.2
Name:
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Sv2c
Name: synaptic vesicle glycoprotein 2c
Synonyms: 4930527L09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 75209
Homologene: 57152
Spire1
Name: spire type actin nucleation factor 1
Synonyms: Spir-1, 6030430B19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 68166
VEGA: 18
Homologene: 35507
Speer3
Name: spermatogenesis associated glutamate (E)-rich protein 3
Synonyms: SPEER-3, 4933405P08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 71026
Homologene: 69402
Nectin4
Name: nectin cell adhesion molecule 4
Synonyms: nectin 4, Prr4, 1200017F15Rik, Pvrl4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 71740
Homologene: 32744
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 78,682,020 bp
  • T to C, chromosome 1 at 171,385,653 bp
  • T to C, chromosome 2 at 104,433,790 bp
  • C to T, chromosome 3 at 87,089,151 bp
  • A to T, chromosome 4 at 113,236,493 bp
  • T to C, chromosome 4 at 115,518,431 bp
  • C to A, chromosome 4 at 130,135,811 bp
  • A to G, chromosome 4 at 134,073,784 bp
  • A to G, chromosome 4 at 137,528,912 bp
  • T to A, chromosome 5 at 13,795,431 bp
  • A to T, chromosome 5 at 75,174,981 bp
  • T to A, chromosome 6 at 56,730,687 bp
  • C to T, chromosome 7 at 44,775,442 bp
  • A to T, chromosome 7 at 106,330,270 bp
  • A to G, chromosome 7 at 141,092,279 bp
  • T to G, chromosome 8 at 18,703,544 bp
  • C to T, chromosome 8 at 109,907,613 bp
  • C to T, chromosome 8 at 121,799,549 bp
  • AGAGGTAGTCACTGA to AGA, chromosome 9 at 21,756,215 bp
  • A to T, chromosome 9 at 66,066,307 bp
  • C to T, chromosome 12 at 80,874,460 bp
  • A to G, chromosome 13 at 95,976,588 bp
  • A to T, chromosome 14 at 70,521,425 bp
  • C to T, chromosome 15 at 79,715,879 bp
  • A to G, chromosome 17 at 26,015,942 bp
  • A to C, chromosome 18 at 67,491,347 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3028 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040544-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.