Strain Name:
C57BL/6J-MtgxR3053Btlr/Mmmh
Stock Number:
040562-MU
Citation ID:
RRID:MMRRC_040562-MU
Other Names:
R3053 (G1), C57BL/6J-MtgxR3053Btlr
Major Collection:

Strain Information

Ubxn2a
Name: UBX domain protein 2A
Synonyms: 6330407P03Rik, Ubxd4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 217379
Homologene: 17076
Ppm1b
Name: protein phosphatase 1B, magnesium dependent, beta isoform
Synonyms: PP2CB
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 19043
VEGA: 17
HGNC: HGNC:9276
Homologene: 2027
Tacc2
Name: transforming, acidic coiled-coil containing protein 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 57752
Homologene: 5087
Xpo6
Name: exportin 6
Synonyms: Ranbp20, C230091E20Rik, 2610005L19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 74204
Homologene: 12544
Arap2
Name: ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 2
Synonyms: Centd1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 212285
Homologene: 9064
Dmtf1
Name: cyclin D binding myb like transcription factor 1
Synonyms: Dmp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 23857
Homologene: 8017
Rapgef1
Name: Rap guanine nucleotide exchange factor (GEF) 1
Synonyms: C3G, 4932418O06Rik, Grf2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 107746
HGNC: HGNC:4568
Homologene: 50501
Trp53bp2
Name: transformation related protein 53 binding protein 2
Synonyms: ASPP2, 53BP2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 209456
Homologene: 3959
Lama5
Name: laminin, alpha 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 16776
HGNC: HGNC:6485
Homologene: 4060
Pole
Name: polymerase (DNA directed), epsilon
Synonyms: pol-epsilon
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 18973
HGNC: HGNC:9177
Homologene: 4539
Cldn8
Name: claudin 8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 54420
HGNC: HGNC:2050
Homologene: 8117
R3hcc1l
Name: R3H domain and coiled-coil containing 1 like
Synonyms: 1700036B12Rik, D19Ertd386e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 52013
Homologene: 8707
Slc15a4
Name: solute carrier family 15, member 4
Synonyms: C130069N12Rik, PTR4, PHT1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100561
Homologene: 26432
Skiv2l
Name: superkiller viralicidic activity 2-like (S. cerevisiae)
Synonyms: Ski2w, 4930534J06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 108077
Homologene: 123971
Ryr1
Name: ryanodine receptor 1, skeletal muscle
Synonyms: calcium release channel isoform 1, Ryr, skrr
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 20190
Homologene: 68069
Chchd2l
Name: coiled-coil-helix-coiled-coil-helix domain containing 2-like
Synonyms: 2410018M08Rik, Zbed5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 71970
Homologene: 84838
Spats2l
Name: spermatogenesis associated, serine-rich 2-like
Synonyms: A230104H11Rik, 2810022L02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 67198
Homologene: 56711
Or1n1
Name: olfactory receptor family 1 subfamily N member 1
Synonyms: GA_x6K02T2NLDC-33554926-33553994, MOR127-2, Olfr351
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258944
HGNC: HGNC:8221
Homologene: 10583
Dner
Name: delta/notch-like EGF repeat containing
Synonyms: BET, A930026D19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 227325
Homologene: 26722
Agl
Name: amylo-1,6-glucosidase, 4-alpha-glucanotransferase
Synonyms: 9430004C13Rik, 9630046L06Rik, 1110061O17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 77559
HGNC: HGNC:321
Homologene: 536
Adcy5
Name: adenylate cyclase 5
Synonyms: AC5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 224129
VEGA: 16
HGNC: HGNC:236
Homologene: 11213
Bcl11b
Name: B cell leukemia/lymphoma 11B
Synonyms: COUP-TF interacting protein 2, CTIP2, B630002E05Rik, Rit1, 9130430L19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 58208
Homologene: 10974
Nrxn3
Name: neurexin III
Synonyms: neurexin III beta, neurexin III alpha, neurexin III beta, neurexin III alpha, 9330112C09Rik, D12Bwg0831e, 4933401A11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 18191
HGNC: HGNC:8010
Homologene: 83225
Mical2
Name: microtubule associated monooxygenase, calponin and LIM domain containing 2
Synonyms: 5330438E18Rik, Ebitein1, 4921517J23Rik, Micalcl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 320878
Homologene: 8760
Tmem132d
Name: transmembrane protein 132D
Synonyms: C630028F04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 243274
Homologene: 71684
Ugt2a1
Name: UDP glucuronosyltransferase 2 family, polypeptide A1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 94215
Homologene: 136808
Axdnd1
Name: axonemal dynein light chain domain containing 1
Synonyms: LOC381304, 9430070O13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 77352
Homologene: 52328
Psg18
Name: pregnancy specific beta-1-glycoprotein 18
Synonyms: mmCGM6, Cea-3, Cea3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 26438
HGNC: HGNC:1816
Homologene: 110989
Terf2
Name: telomeric repeat binding factor 2
Synonyms: TRF2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 21750
Homologene: 4133
Cibar1
Name: CBY1 interacting BAR domain containing 1
Synonyms: 6720467C03Rik, Fam92a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 68099
Homologene: 12234
Asb18
Name: ankyrin repeat and SOCS box-containing 18
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 208372
Homologene: 16382
Qars
Name: glutaminyl-tRNA synthetase
Synonyms: 1110018N24Rik, 1200016L19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 97541
HGNC: HGNC:9751
Homologene: 133975
Tbc1d21
Name: TBC1 domain family, member 21
Synonyms: 1700095K08Rik, MgcRabGAP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 74286
VEGA: 9
Homologene: 14033
Gmppa
Name: GDP-mannose pyrophosphorylase A
Synonyms: 1810012N01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 69080
Homologene: 8346
Cyp2a5
Name: cytochrome P450, family 2, subfamily a, polypeptide 5
Synonyms: Coh
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 13087
Homologene: 85917
Tedc1
Name: tubulin epsilon and delta complex 1
Synonyms: 4930427A07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 104732
Homologene: 77129
A530088E08Rik
Name: RIKEN cDNA A530088E08 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 664702
VEGA: 17
Zfp729b
Name: zinc finger protein 729b
Synonyms: AA987161
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 100416706
Homologene: 133713
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 57,900,766 bp
  • A to G, chromosome 1 at 75,441,756 bp
  • G to T, chromosome 1 at 84,384,026 bp
  • T to A, chromosome 1 at 89,992,985 bp
  • T to C, chromosome 1 at 156,341,870 bp
  • T to C, chromosome 1 at 182,453,782 bp
  • T to C, chromosome 2 at 29,724,856 bp
  • T to A, chromosome 2 at 36,859,947 bp
  • C to T, chromosome 2 at 180,183,067 bp
  • T to A, chromosome 3 at 116,791,033 bp
  • G to C, chromosome 4 at 12,157,767 bp
  • G to T, chromosome 5 at 9,129,316 bp
  • A to G, chromosome 5 at 62,748,857 bp
  • A to T, chromosome 5 at 87,474,469 bp
  • A to T, chromosome 5 at 110,289,795 bp
  • A to T, chromosome 5 at 127,596,682 bp
  • A to C, chromosome 5 at 127,792,474 bp
  • A to T, chromosome 5 at 129,902,146 bp
  • C to T, chromosome 7 at 18,349,193 bp
  • A to G, chromosome 7 at 26,842,985 bp
  • A to T, chromosome 7 at 29,053,090 bp
  • G to T, chromosome 7 at 112,311,423 bp
  • T to C, chromosome 7 at 126,104,721 bp
  • G to A, chromosome 7 at 130,625,496 bp
  • A to C, chromosome 8 at 107,079,384 bp
  • T to A, chromosome 9 at 58,363,069 bp
  • T to C, chromosome 9 at 108,509,428 bp
  • T to A, chromosome 12 at 4,891,322 bp
  • A to T, chromosome 12 at 89,255,101 bp
  • T to C, chromosome 12 at 107,916,001 bp
  • G to T, chromosome 12 at 113,156,467 bp
  • A to G, chromosome 13 at 67,593,466 bp
  • T to C, chromosome 16 at 35,303,716 bp
  • A to C, chromosome 16 at 88,562,731 bp
  • T to C, chromosome 17 at 32,403,439 bp
  • G to A, chromosome 17 at 34,844,606 bp
  • G to A, chromosome 17 at 85,013,846 bp
  • T to G, chromosome 19 at 42,562,625 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3053 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040562-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.