Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR3103Btlr/Mmmh
Stock Number:
040577-MU
Citation ID:
RRID:MMRRC_040577-MU
Other Names:
R3103 (G1), C57BL/6J-MtgxR3103Btlr
Major Collection:

Strain Information

Ptch1
Name: patched 1
Synonyms: Ptc, Patched 1, Ptc1, A230106A15Rik, wig
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 19206
HGNC: HGNC:9585
Homologene: 223
Dpagt1
Name: dolichyl-phosphate N-acetylglucosaminephosphotransferase 1
Synonyms: GPT, Dpagt2, Gnpta
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 13478
VEGA: 9
HGNC: HGNC:2995
Homologene: 1058
Trpc2
Name: transient receptor potential cation channel, subfamily C, member 2
Synonyms: TRPC2b, TRPC2a, mTrp2, trp2, Trrp2, 3010009O07Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 22064
Homologene: 135989
Kcmf1
Name: potassium channel modulatory factor 1
Synonyms: clone DEBT-91, 1700094M07Rik, Pmcf
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 74287
Homologene: 99845
Lrp2
Name: low density lipoprotein receptor-related protein 2
Synonyms: Megalin, Gp330, D230004K18Rik, b2b1625.2Clo
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14725
HGNC: HGNC:6694
Homologene: 20952
Usp47
Name: ubiquitin specific peptidase 47
Synonyms: 4930502N04Rik, A630020C16Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 74996
Homologene: 9929
Lrrfip2
Name: leucine rich repeat (in FLII) interacting protein 2
Synonyms: 5133400F20Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 71268
HGNC: HGNC:6703
Homologene: 22476
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 107,377,608 bp
  • T to C, chromosome 2 at 36,793,562 bp
  • C to T, chromosome 2 at 69,431,984 bp
  • A to T, chromosome 2 at 164,333,393 bp
  • T to A, chromosome 2 at 167,026,246 bp
  • A to G, chromosome 3 at 5,399,326 bp
  • G to T, chromosome 3 at 38,891,940 bp
  • G to A, chromosome 4 at 25,007,353 bp
  • G to T, chromosome 4 at 116,524,249 bp
  • T to A, chromosome 4 at 117,760,608 bp
  • T to C, chromosome 4 at 122,836,307 bp
  • T to C, chromosome 5 at 31,061,674 bp
  • T to A, chromosome 5 at 32,328,029 bp
  • A to G, chromosome 6 at 72,861,847 bp
  • T to C, chromosome 6 at 77,653,144 bp
  • A to G, chromosome 7 at 19,087,654 bp
  • A to G, chromosome 7 at 28,610,984 bp
  • C to T, chromosome 7 at 29,074,948 bp
  • T to A, chromosome 7 at 42,266,643 bp
  • T to C, chromosome 7 at 79,121,736 bp
  • A to G, chromosome 7 at 100,395,252 bp
  • A to T, chromosome 7 at 102,095,234 bp
  • T to C, chromosome 7 at 102,659,481 bp
  • T to A, chromosome 7 at 112,067,078 bp
  • C to T, chromosome 8 at 15,917,405 bp
  • T to A, chromosome 9 at 32,728,798 bp
  • A to G, chromosome 9 at 44,327,995 bp
  • A to G, chromosome 9 at 78,064,452 bp
  • A to G, chromosome 9 at 108,837,139 bp
  • A to T, chromosome 9 at 111,222,210 bp
  • A to G, chromosome 10 at 49,240,772 bp
  • A to G, chromosome 10 at 59,438,891 bp
  • A to G, chromosome 10 at 85,993,422 bp
  • C to A, chromosome 11 at 70,008,869 bp
  • A to T, chromosome 11 at 72,257,388 bp
  • G to A, chromosome 11 at 74,196,075 bp
  • G to T, chromosome 11 at 80,157,033 bp
  • T to A, chromosome 12 at 91,019,344 bp
  • T to A, chromosome 13 at 61,250,958 bp
  • T to C, chromosome 13 at 63,543,209 bp
  • A to T, chromosome 13 at 96,482,506 bp
  • C to A, chromosome 13 at 112,530,351 bp
  • T to C, chromosome 14 at 75,747,201 bp
  • A to T, chromosome 15 at 100,525,600 bp
  • T to C, chromosome 16 at 33,468,085 bp
  • A to G, chromosome 16 at 36,894,849 bp
  • T to C, chromosome 16 at 97,422,001 bp
  • A to T, chromosome 18 at 76,859,278 bp
  • T to C, chromosome 18 at 77,871,777 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3103 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040577-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.