Strain Name:
C57BL/6J-MtgxR3159Btlr/Mmmh
Stock Number:
040610-MU
Citation ID:
RRID:MMRRC_040610-MU
Other Names:
R3159 (G1), C57BL/6J-MtgxR3159Btlr
Major Collection:

Strain Information

Slit1
Name: slit guidance ligand 1
Synonyms: Slil1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 20562
Homologene: 2302
Zranb3
Name: zinc finger, RAN-binding domain containing 3
Synonyms: 4933425L19Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226409
Homologene: 32770
Hmgcr
Name: 3-hydroxy-3-methylglutaryl-Coenzyme A reductase
Synonyms: 3-hydroxy-3-methylglutaryl-CoA reductase, HMG-CoAR, Red
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 15357
HGNC: HGNC:5006
Homologene: 30994
Ociad1
Name: OCIA domain containing 1
Synonyms: Emi2, Imi2, 6030432N09Rik, B230209J16Rik, expressed during mesenchymal induction 2, TPA018, Asrij
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 68095
Homologene: 9866
Gphn
Name: gephyrin
Synonyms: geph, 5730552E08Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 268566
VEGA: 12
Homologene: 10820
Jade3
Name: jade family PHD finger 3
Synonyms: Phf16
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 382207
Homologene: 40981
Bltp1
Name: bridge-like lipid transfer protein family member 1
Synonyms: 4932438A13Rik, Tweek, FSA
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229227
Homologene: 52105
Zp3
Name: zona pellucida glycoprotein 3
Synonyms: Zp-3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 22788
Homologene: 5178
Smu1
Name: smu-1 suppressor of mec-8 and unc-52 homolog (C. elegans)
Synonyms: 2610203K23Rik, SMU-1, 2600001O03Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 74255
Homologene: 10079
Rbm48
Name: RNA binding motif protein 48
Synonyms: C030048B08Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 269623
Homologene: 12944
Zfp667
Name: zinc finger protein 667
Synonyms: A830025F02Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 384763
Homologene: 23343
Galnt12
Name: polypeptide N-acetylgalactosaminyltransferase 12
Synonyms: A630062B03Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230145
Homologene: 11637
Tgfb3
Name: transforming growth factor, beta 3
Synonyms: Tgfb-3
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 21809
VEGA: 12
Homologene: 2433
Tns2
Name: tensin 2
Synonyms: nep, Tenc1, nph
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 209039
VEGA: 15
Homologene: 37077
Dmpk
Name: dystrophia myotonica-protein kinase
Synonyms: DM, Dm15
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 13400
HGNC: HGNC:2933
Homologene: 3247
Myo1g
Name: myosin IG
Synonyms: E430002D17Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 246177
Homologene: 27996
Sh3rf1
Name: SH3 domain containing ring finger 1
Synonyms: Posh, 2200003J05Rik, Sh3md2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 59009
Homologene: 10988
Dscam
Name: DS cell adhesion molecule
Synonyms: Down syndrome cell adhesion molecule, 4932410A21Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 13508
HGNC: HGNC:3039
Homologene: 74393
Gxylt1
Name: glucoside xylosyltransferase 1
Synonyms: LOC223827, LOC382997, Glt8d3
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223827
Homologene: 28259
Slc8a3
Name: solute carrier family 8 (sodium/calcium exchanger), member 3
Synonyms: Ncx3
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 110893
Homologene: 62645
Ccdc146
Name: coiled-coil domain containing 146
Synonyms: 4930528G09Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 75172
Homologene: 67159
Rfx6
Name: regulatory factor X, 6
Synonyms: Rfxdc1, 4930572O07Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 320995
Homologene: 18318
Col9a1
Name: collagen, type IX, alpha 1
Synonyms: Col9a-1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12839
HGNC: HGNC:2217
Homologene: 1393
Shank2
Name: SH3 and multiple ankyrin repeat domains 2
Synonyms: ProSAP1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 210274
Homologene: 105965
Polg2
Name: polymerase (DNA directed), gamma 2, accessory subunit
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 50776
HGNC: HGNC:9180
Homologene: 5221
Celsr3
Name: cadherin, EGF LAG seven-pass G-type receptor 3
Synonyms: Fmi1, flamingo, Adgrc3
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 107934
HGNC: HGNC:3230
Homologene: 1077
Slc9b1
Name: solute carrier family 9, subfamily B (NHA1, cation proton antiporter 1), member 1
Synonyms: 1700094G20Rik, 4933424B12Rik, Nhedc1, 4933425K02Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 74446
Homologene: 49914
Isg20
Name: interferon-stimulated protein
Synonyms: HEM45, 20kDa, 1600023I01Rik, DnaQl, 2010107M23Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 57444
HGNC: HGNC:6130
Homologene: 31081
Cdk15
Name: cyclin dependent kinase 15
Synonyms: Pftk2, Als2cr7
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 271697
Homologene: 64641
Mark1
Name: MAP/microtubule affinity regulating kinase 1
Synonyms: B930025N23Rik, Emk3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226778
HGNC: HGNC:6896
Homologene: 49552
Mmp11
Name: matrix metallopeptidase 11
Synonyms: ST3, stromelysin 3, Stmy3
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 17385
HGNC: HGNC:7157
Homologene: 38116
Or5t7
Name: olfactory receptor family 5 subfamily T member 17
Synonyms: Olfr1086, MOR179-2, GA_x6K02T2Q125-48168771-48167839
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258585
Homologene: 73975
Sav1
Name: salvador family WW domain containing 1
Synonyms: Salv, WW45, 1700040G09Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 64010
VEGA: 12
Homologene: 32517
Hsd17b2
Name: hydroxysteroid (17-beta) dehydrogenase 2
Synonyms: 17 HSD type 2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 15486
HGNC: HGNC:5211
Homologene: 99709
Ccr4
Name: C-C motif chemokine receptor 4
Synonyms: Cmkbr4, CC CKR-4
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 12773
VEGA: 9
HGNC: HGNC:1605
Homologene: 21135
Gm5145
Name: predicted pseudogene 5145
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 381065
VEGA: 17
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 24,221,564 bp
  • T to C, chromosome 1 at 59,301,281 bp
  • A to G, chromosome 1 at 127,972,949 bp
  • A to T, chromosome 1 at 184,908,387 bp
  • A to G, chromosome 2 at 86,676,511 bp
  • T to C, chromosome 3 at 36,959,415 bp
  • G to A, chromosome 3 at 135,371,845 bp
  • T to A, chromosome 4 at 40,754,529 bp
  • A to G, chromosome 4 at 47,104,264 bp
  • A to T, chromosome 5 at 3,596,105 bp
  • T to C, chromosome 5 at 21,399,792 bp
  • A to T, chromosome 5 at 73,310,345 bp
  • T to A, chromosome 5 at 135,987,459 bp
  • T to C, chromosome 7 at 6,306,000 bp
  • A to G, chromosome 7 at 19,093,019 bp
  • C to A, chromosome 7 at 78,914,453 bp
  • C to A, chromosome 7 at 144,081,874 bp
  • C to T, chromosome 8 at 61,226,287 bp
  • A to C, chromosome 8 at 117,758,752 bp
  • T to C, chromosome 9 at 108,827,710 bp
  • G to T, chromosome 9 at 114,492,282 bp
  • A to T, chromosome 10 at 51,726,720 bp
  • C to T, chromosome 10 at 75,927,114 bp
  • T to C, chromosome 10 at 92,999,056 bp
  • G to T, chromosome 11 at 6,514,527 bp
  • A to C, chromosome 11 at 106,768,337 bp
  • A to T, chromosome 12 at 69,984,552 bp
  • T to C, chromosome 12 at 78,492,029 bp
  • C to A, chromosome 12 at 81,314,992 bp
  • A to T, chromosome 12 at 86,058,986 bp
  • C to T, chromosome 13 at 96,665,847 bp
  • A to G, chromosome 15 at 93,245,032 bp
  • C to T, chromosome 15 at 102,108,934 bp
  • T to C, chromosome 16 at 96,678,510 bp
  • A to G, chromosome 17 at 20,570,893 bp
  • A to G, chromosome 19 at 41,604,373 bp
  • A to G, chromosome X at 20,479,544 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3159 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040610-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.