Strain Name:
C57BL/6J-MtgxR3404Btlr/Mmmh
Stock Number:
040622-MU
Citation ID:
RRID:MMRRC_040622-MU
Other Names:
R3404 (G1), C57BL/6J-MtgxR3404Btlr
Major Collection:

Gene Information

Pzp
Name: PZP, alpha-2-macroglobulin like
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 11287
Homologene: 104112
Mycbp2
Name: MYC binding protein 2, E3 ubiquitin protein ligase
Synonyms: Pam, Phr1, C130061D10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 105689
Homologene: 9005
Ubap2l
Name: ubiquitin-associated protein 2-like
Synonyms: 3110083O19Rik, NICE-4, A430103N23Rik, 4932431F02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 74383
Homologene: 136291
Zfp609
Name: zinc finger protein 609
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 214812
Homologene: 72220
Ticrr
Name: TOPBP1-interacting checkpoint and replication regulator
Synonyms: 5730590G19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 77011
Homologene: 67120
Patj
Name: PATJ, crumbs cell polarity complex component
Synonyms: Cipp, Inadl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 12695
Homologene: 72199
Bnc2
Name: basonuclin 2
Synonyms: 5031434M05Rik, 8430420F16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 242509
Homologene: 18243
Orc4
Name: origin recognition complex, subunit 4
Synonyms: Orc4l, Orc4, mMmORC4, Orc4P
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 26428
HGNC: HGNC:8490
Homologene: 8059
Ltn1
Name: listerin E3 ubiquitin protein ligase 1
Synonyms: Rnf160, Listerin, Zfp294, 4930528H02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 78913
VEGA: 16
Homologene: 32272
Senp7
Name: SUMO1/sentrin specific peptidase 7
Synonyms: 2900036C23Rik, 2810413I22Rik, 6030449K19Rik, 2410152H17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 66315
Homologene: 10778
Mki67
Name: antigen identified by monoclonal antibody Ki 67
Synonyms: Ki67, D630048A14Rik, Ki-67
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 17345
HGNC: HGNC:7107
Homologene: 1814
Cdc27
Name: cell division cycle 27
Synonyms: APC3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 217232
HGNC: HGNC:1728
Homologene: 960
Uvssa
Name: UV stimulated scaffold protein A
Synonyms: D330017J19Rik, 4933407H18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 71101
Homologene: 13807
Vps13b
Name: vacuolar protein sorting 13B
Synonyms: 1810042B05Rik, C330002D13Rik, Coh1, 2310042E16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 666173
VEGA: 15
HGNC: HGNC:2183
Homologene: 49516
Alms1
Name: ALMS1, centrosome and basal body associated
Synonyms: Alstrom syndrome 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 236266
HGNC: HGNC:428
Homologene: 49406
Fezf1
Name: Fez family zinc finger 1
Synonyms: Zfp312-like, 3110069A13Rik, Fez
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 73191
Homologene: 19252
Apc
Name: APC, WNT signaling pathway regulator
Synonyms: CC1, adenomatosis polyposis coli, Min
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 11789
HGNC: HGNC:583
Homologene: 30950
Hspa13
Name: heat shock protein 70 family, member 13
Synonyms: 60kDa, Stch, 1600002I10Rik, B230217N24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 110920
Homologene: 5062
Baz1a
Name: bromodomain adjacent to zinc finger domain 1A
Synonyms: Acf1, Wcrf180, Gtl5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 217578
HGNC: HGNC:960
Homologene: 45654
Trim33
Name: tripartite motif-containing 33
Synonyms: Tif1g, ectodermin, Ecto, 8030451N04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 94093
Homologene: 9296
Alg12
Name: asparagine-linked glycosylation 12 (alpha-1,6-mannosyltransferase)
Synonyms: ECM39, mannosyltransferase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 223774
Homologene: 36269
Brip1
Name: BRCA1 interacting protein C-terminal helicase 1
Synonyms: BACH1, 8030460J03Rik, 3110009N10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 237911
Homologene: 32766
Mfng
Name: MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
Synonyms: manic fringe
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 17305
HGNC: HGNC:7038
Homologene: 1803
Pcdh17
Name: protocadherin 17
Synonyms: LOC219228, C030033F14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 219228
VEGA: 14
Homologene: 8700
Dnhd1
Name: dynein heavy chain domain 1
Synonyms: 8030491N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 77505
Homologene: 131117
Immp2l
Name: IMP2 inner mitochondrial membrane peptidase-like (S. cerevisiae)
Synonyms: IMP2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 93757
Homologene: 6607
Itgam
Name: integrin alpha M
Synonyms: CR3, Mac-1, Ly-40, CD11b/CD18, Mac-1 alpha, CD11B (p170), Mac-1a, Cd11b, complement receptor type 3, F730045J24Rik, complement component receptor 3 alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 16409
HGNC: HGNC:6149
Homologene: 526
Cyp2c66
Name: cytochrome P450, family 2, subfamily c, polypeptide 66
Synonyms: 2010301M18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 69888
HGNC: HGNC:2623
Homologene: 133566
Nlrp2
Name: NLR family, pyrin domain containing 2
Synonyms: E330007A02Rik, PYPAF2, Nalp2, Pan1, Nbs1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 232827
Homologene: 56789
Adcy8
Name: adenylate cyclase 8
Synonyms: AC8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 11514
VEGA: 15
HGNC: HGNC:239
Homologene: 37443
Ankar
Name: ankyrin and armadillo repeat containing
Synonyms: 4932422E22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 319695
Homologene: 85163
Dnaic1
Name: dynein, axonemal, intermediate chain 1
Synonyms: 1110066F04Rik, b2b1526Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 68922
HGNC: HGNC:2954
Homologene: 8122
Gsdma
Name: gasdermin A
Synonyms: Gsdm1, H312E, Gsdm, Gsdma1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 57911
Homologene: 10962
Ube4a
Name: ubiquitination factor E4A
Synonyms: 4732444G18Rik, 9930123J21Rik, UFD2b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 140630
Homologene: 3517
Rbfox3
Name: RNA binding protein, fox-1 homolog (C. elegans) 3
Synonyms: Neuna60, Hrnbp3, NeuN, D11Bwg0517e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 52897
Homologene: 134629
Prkd1
Name: protein kinase D1
Synonyms: Pkcm, PKD1, Prkcm
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 18760
VEGA: 12
HGNC: HGNC:9407
Homologene: 55680
Tdrd9
Name: tudor domain containing 9
Synonyms: 4930441E05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 74691
Homologene: 14311
Bdkrb2
Name: bradykinin receptor, beta 2
Synonyms: kinin B2, B(2), B2, BK2R, B2R
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 12062
VEGA: 12
HGNC: HGNC:1030
Homologene: 519
Bcorl1
Name: BCL6 co-repressor-like 1
Synonyms: 6720425J07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Chemically Induced
NCBI: 320376
Homologene: 41458
Snx31
Name: sorting nexin 31
Synonyms: 4631426E05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 66696
Homologene: 23551
Rnf146
Name: ring finger protein 146
Synonyms: 2610509H23Rik, Iduna
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 68031
Homologene: 12227
Ct55
Name: cancer/testis antigen 55
Synonyms: 4930502E18Rik, Ct55
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Chemically Induced
NCBI: 75013
Homologene: 137390
Hemk1
Name: HemK methyltransferase family member 1
Synonyms: 2310008M14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 69536
Homologene: 6815
Zfp729b
Name: zinc finger protein 729b
Synonyms: AA987161
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 100416706
Homologene: 133713
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 72,643,093 bp
  • G to A, chromosome 2 at 48,937,489 bp
  • T to C, chromosome 3 at 90,038,850 bp
  • A to G, chromosome 3 at 103,321,559 bp
  • A to T, chromosome 4 at 41,603,246 bp
  • G to T, chromosome 4 at 84,546,241 bp
  • T to C, chromosome 4 at 98,523,381 bp
  • G to T, chromosome 5 at 33,389,818 bp
  • A to T, chromosome 6 at 23,247,284 bp
  • C to A, chromosome 6 at 85,667,963 bp
  • G to A, chromosome 6 at 128,513,806 bp
  • T to C, chromosome 7 at 5,319,287 bp
  • G to C, chromosome 7 at 79,694,791 bp
  • C to T, chromosome 7 at 105,694,761 bp
  • A to G, chromosome 7 at 128,070,703 bp
  • T to A, chromosome 7 at 135,707,475 bp
  • A to G, chromosome 9 at 44,929,687 bp
  • A to T, chromosome 9 at 65,701,172 bp
  • G to A, chromosome 9 at 107,337,216 bp
  • A to G, chromosome 10 at 29,347,428 bp
  • T to A, chromosome 11 at 86,143,263 bp
  • T to C, chromosome 11 at 98,673,138 bp
  • T to C, chromosome 11 at 104,507,200 bp
  • T to A, chromosome 11 at 118,496,457 bp
  • T to A, chromosome 12 at 41,110,847 bp
  • C to T, chromosome 12 at 50,648,904 bp
  • C to T, chromosome 12 at 54,916,989 bp
  • T to C, chromosome 12 at 105,592,496 bp
  • A to G, chromosome 12 at 112,027,160 bp
  • T to G, chromosome 13 at 67,591,164 bp
  • T to A, chromosome 14 at 84,446,622 bp
  • C to A, chromosome 14 at 103,200,114 bp
  • T to C, chromosome 15 at 35,926,054 bp
  • C to T, chromosome 15 at 36,525,653 bp
  • C to T, chromosome 15 at 64,699,600 bp
  • A to C, chromosome 15 at 78,760,900 bp
  • A to T, chromosome 15 at 88,814,579 bp
  • T to A, chromosome 16 at 56,188,277 bp
  • C to A, chromosome 16 at 75,758,026 bp
  • A to T, chromosome 16 at 87,416,215 bp
  • A to G, chromosome 18 at 34,313,602 bp
  • T to C, chromosome 19 at 39,163,327 bp
  • T to A, chromosome X at 48,371,007 bp
  • A to C, chromosome X at 53,727,354 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3404 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040622-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.