Strain Name:
C57BL/6J-MtgxR3411Btlr/Mmmh
Stock Number:
040629-MU
Citation ID:
RRID:MMRRC_040629-MU
Other Names:
R3411 (G1), C57BL/6J-MtgxR3411Btlr
Major Collection:

Gene Information

Htr1b
Name: 5-hydroxytryptamine (serotonin) receptor 1B
Synonyms: 5-HT1B receptor, 5-HT1B receptor, 5HT1B receptor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 15551
HGNC: HGNC:5287
Homologene: 669
Mast2
Name: microtubule associated serine/threonine kinase 2
Synonyms: Mtssk, MAST205
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 17776
Homologene: 7428
Aff1
Name: AF4/FMR2 family, member 1
Synonyms: Mllt2h, Af4, 9630032B01Rik, Rob
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 17355
HGNC: HGNC:7135
Homologene: 4340
Wdr7
Name: WD repeat domain 7
Synonyms: TGF-beta resistance associated gene, TRAG
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 104082
VEGA: 18
Homologene: 11408
Klhl7
Name: kelch-like 7
Synonyms: Klhl6, D5Ertd363e, 2700038B03Rik, SBBI26
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 52323
Homologene: 10317
Arid4a
Name: AT rich interactive domain 4A (RBP1-like)
Synonyms: A630067N03Rik, Rbbp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 238247
HGNC: HGNC:9885
Homologene: 11303
Usp53
Name: ubiquitin specific peptidase 53
Synonyms: mbo, Phxr3, Sp6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 99526
Homologene: 34521
Snw1
Name: SNW domain containing 1
Synonyms: SKIP, 2310008B08Rik, SNW1, Skiip, NCoA-62
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 66354
VEGA: 12
Homologene: 56557
Frem1
Name: Fras1 related extracellular matrix protein 1
Synonyms: eyem02Jus, heb, eyes2, crf11, QBRICK
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 329872
Homologene: 27049
Smu1
Name: smu-1 suppressor of mec-8 and unc-52 homolog (C. elegans)
Synonyms: 2600001O03Rik, 2610203K23Rik, SMU-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 74255
Homologene: 10079
Tenm4
Name: teneurin transmembrane protein 4
Synonyms: Doc4, ELM2, l7Rn3, Odz4, Ten-m4, l(7)-3Rn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 23966
Homologene: 8034
Hjurp
Name: Holliday junction recognition protein
Synonyms: A730008H23Rik, C330011F01Rik, 6430706D22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 381280
Homologene: 10184
Sptb
Name: spectrin beta, erythrocytic
Synonyms: spectrin R, brain erythroid spectrin (235E), LOC383567, Spnb-1, Spnb1, D330027P03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 20741
Homologene: 295
Adgra3
Name: adhesion G protein-coupled receptor A3
Synonyms: Gpr125, 3830613O22Rik, Tem5-like
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 70693
Homologene: 19235
Apc
Name: APC, WNT signaling pathway regulator
Synonyms: CC1, Min
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 11789
HGNC: HGNC:583
Homologene: 30950
Rpn2
Name: ribophorin II
Synonyms: Rpn-2, 1300012C06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 20014
Homologene: 2214
Atp5o
Name: ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit
Synonyms: D12Wsu28e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 28080
HGNC: HGNC:850
Homologene: 1283
Dnpep
Name: aspartyl aminopeptidase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 13437
HGNC: HGNC:2981
Homologene: 6110
Mcm6
Name: minichromosome maintenance complex component 6
Synonyms: Mcmd6, D1Wsu22e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 17219
HGNC: HGNC:6949
Homologene: 4322
Hacd3
Name: 3-hydroxyacyl-CoA dehydratase 3
Synonyms: Hspc121, Ptplad1, B-ind1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 57874
VEGA: 9
Homologene: 9494
Ccdc88a
Name: coiled coil domain containing 88A
Synonyms: D130005J21Rik, GIV, A430106J12Rik, C330012F17Rik, Girdin, HkRP1, C130096N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 108686
Homologene: 41180
Golga2
Name: golgi autoantigen, golgin subfamily a, 2
Synonyms: GM130
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 99412
HGNC: HGNC:4425
Homologene: 3300
Kalrn
Name: kalirin, RhoGEF kinase
Synonyms: 2210407G14Rik, Hapip, E530005C20Rik, LOC224126
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 545156
HGNC: HGNC:4814
Homologene: 57160
Rhbdf2
Name: rhomboid 5 homolog 2
Synonyms: 4732465I17Rik, cub, iRhom2, Rhbdl6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 217344
Homologene: 11612
Gm6802
Name: predicted gene 6802
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: null
HGNC: null
Homologene: null
Dnah1
Name: dynein, axonemal, heavy chain 1
Synonyms: B230373P09Rik, E030034C22Rik, Dnahc1, MDHC7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 110084
VEGA: 14
HGNC: HGNC:2940
Homologene: 67131
Slc16a4
Name: solute carrier family 16 (monocarboxylic acid transporters), member 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 229699
Homologene: 74529
Mslnl
Name: mesothelin-like
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 328783
VEGA: 17
HGNC: null
Homologene: 18633
Ttn
Name: titin
Synonyms: shru, connectin, mdm, 2310057K23Rik, D330041I19Rik, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, L56, 2310074I15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 22138
Homologene: 130650
Egfem1
Name: EGF-like and EMI domain containing 1
Synonyms: 6130401L20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 75740
Homologene: 135950
Kcp
Name: kielin/chordin-like protein
Synonyms: KCP, LOC333088, Crim2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 333088
Homologene: 87817
Pdzd8
Name: PDZ domain containing 8
Synonyms: Pdzk8, A630041P07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 107368
VEGA: 19
Homologene: 14879
Hcrtr2
Name: hypocretin (orexin) receptor 2
Synonyms: mOX2bR, mOX2aR, OX2r, mOXR2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 387285
HGNC: HGNC:4849
Homologene: 1168
Apcs
Name: serum amyloid P-component
Synonyms: Sap
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 20219
HGNC: HGNC:584
Homologene: 123932
Adamts16
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 16
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 271127
Homologene: 15146
Otoa
Name: otoancorin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 246190
Homologene: 71803
Ripk4
Name: receptor-interacting serine-threonine kinase 4
Synonyms: Ankrd3, DIk, PKK, RIP4, ANKK2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 72388
HGNC: HGNC:496
Homologene: 10772
Ccdc150
Name: coiled-coil domain containing 150
Synonyms: 4930511H11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 78016
Homologene: 15814
Grik5
Name: glutamate receptor, ionotropic, kainate 5 (gamma 2)
Synonyms: KA2, GluRgamma2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 14809
HGNC: HGNC:4583
Homologene: 1578
Gpat2
Name: glycerol-3-phosphate acyltransferase 2, mitochondrial
Synonyms: Gpat2, A530057A03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 215456
Homologene: 19037
Serpina3n
Name: serine (or cysteine) peptidase inhibitor, clade A, member 3N
Synonyms: alpha-1 antiproteinase, Spi2.2, Spi2/eb.4, antitrypsin, Spi2-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 20716
HGNC: HGNC:16
Homologene: 111129
Olfr1453
Name: olfactory receptor 1453
Synonyms: GA_x6K02T2RE5P-3357666-3356743, MOR202-6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 258695
HGNC: null
Homologene: 120159
Klra17
Name: killer cell lectin-like receptor, subfamily A, member 17
Synonyms: Ly49q1, Ly49Q, Ly-49Q
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 170733
HGNC: null
Homologene: null
Olfr494
Name: olfactory receptor 494
Synonyms: GA_x6K02T2PBJ9-10697517-10698461, MOR204-10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 258732
HGNC: null
Homologene: 133602
Aox4
Name: aldehyde oxidase 4
Synonyms: 2310003G12Rik, AOH2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 71872
HGNC: null
Homologene: 70273
Fam120b
Name: family with sequence similarity 120, member B
Synonyms: 4932442K08Rik, CCPG
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 67544
VEGA: 17
Homologene: 13033
Sypl2
Name: synaptophysin-like 2
Synonyms: Mg29
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 17306
Homologene: 7421
Bank1
Name: B cell scaffold protein with ankyrin repeats 1
Synonyms: A530094C12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 242248
Homologene: 9926
Olfr1510
Name: olfactory receptor 1510
Synonyms: GA_x6K02T2RJGY-583652-584608, MOR223-6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 258423
HGNC: HGNC:8170
Homologene: 87788
Vmn2r65
Name: vomeronasal 2, receptor 65
Synonyms: ENSMUSG00000070600
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 100009609
HGNC: null
Homologene: null
Nlrp4c
Name: NLR family, pyrin domain containing 4C
Synonyms: Nalp-alpha, Rnh2, Nalp4c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 83564
Homologene: 75315
Olfr1047
Name: olfactory receptor 1047
Synonyms: GA_x6K02T2Q125-47703682-47702723, MOR188-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 259014
HGNC: null
Homologene: 133664
Adam4
Name: a disintegrin and metallopeptidase domain 4
Synonyms: tMDCV
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 11498
HGNC: null
Homologene: 86950
Pcdhb6
Name: protocadherin beta 6
Synonyms: Pcdhb5B, PcdhbF
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 93877
HGNC: null
Homologene: 62177
Armcx4
Name: armadillo repeat containing, X-linked 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Chemically Induced
NCBI: 100503043
Homologene: 131587
Adgra1
Name: adhesion G protein-coupled receptor A1
Synonyms: 2900059M17Rik, Gpr123, D7Ertd680e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 52389
Homologene: 18582
Mfsd9
Name: major facilitator superfamily domain containing 9
Synonyms: 4931419K03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 211798
Homologene: 13100
Psmc3
Name: proteasome (prosome, macropain) 26S subunit, ATPase 3
Synonyms: Tat binding protein 1, TBP-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 19182
HGNC: HGNC:9549
Homologene: 2097
AI429214
Name: expressed sequence AI429214
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 621080
Homologene: 82747
Styxl1
Name: serine/threonine/tyrosine interacting-like 1
Synonyms: Dusp24, 1700011C14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 76571
Homologene: 9378
Tal2
Name: T cell acute lymphocytic leukemia 2
Synonyms: bHLHa19
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 21350
Homologene: 3954
D830014E11Rik
Name: RIKEN cDNA D830014E11 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 320751
Ensembl: null
VEGA: 5
HGNC: null
Homologene: null
Six4
Name: sine oculis-related homeobox 4
Synonyms: TrexBF, AREC3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 20474
Homologene: 69089
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 40,786,225 bp
  • A to G, chromosome 1 at 54,356,773 bp
  • T to C, chromosome 1 at 58,239,106 bp
  • A to G, chromosome 1 at 75,316,626 bp
  • GT to GTT, chromosome 1 at 88,266,524 bp
  • T to C, chromosome 1 at 128,351,585 bp
  • T to C, chromosome 1 at 172,894,563 bp
  • C to A, chromosome 2 at 32,302,942 bp
  • A to C, chromosome 2 at 76,942,405 bp
  • T to C, chromosome 2 at 86,228,642 bp
  • A to C, chromosome 2 at 91,055,918 bp
  • G to A, chromosome 2 at 127,428,291 bp
  • C to A, chromosome 2 at 157,290,652 bp
  • C to T, chromosome 3 at 29,583,021 bp
  • G to A, chromosome 3 at 107,300,872 bp
  • C to T, chromosome 3 at 108,216,729 bp
  • T to C, chromosome 3 at 122,949,858 bp
  • G to A, chromosome 3 at 136,247,773 bp
  • T to C, chromosome 4 at 40,752,008 bp
  • A to G, chromosome 4 at 53,785,843 bp
  • T to C, chromosome 4 at 82,963,179 bp
  • C to G, chromosome 4 at 116,310,910 bp
  • G to T, chromosome 5 at 24,138,321 bp
  • A to G, chromosome 5 at 50,001,930 bp
  • T to A, chromosome 5 at 103,754,706 bp
  • A to T, chromosome 5 at 105,826,706 bp
  • T to C, chromosome 5 at 135,765,764 bp
  • T to C, chromosome 6 at 29,482,846 bp
  • T to A, chromosome 6 at 129,874,846 bp
  • A to G, chromosome 7 at 6,092,570 bp
  • T to C, chromosome 7 at 25,062,972 bp
  • T to C, chromosome 7 at 84,946,688 bp
  • T to C, chromosome 7 at 96,852,530 bp
  • A to T, chromosome 7 at 108,368,344 bp
  • A to G, chromosome 7 at 121,122,043 bp
  • T to C, chromosome 7 at 139,847,703 bp
  • C to T, chromosome 8 at 36,993,917 bp
  • A to G, chromosome 9 at 64,991,094 bp
  • A to G, chromosome 9 at 76,233,008 bp
  • A to C, chromosome 9 at 81,632,041 bp
  • T to A, chromosome 11 at 29,486,006 bp
  • A to G, chromosome 11 at 116,604,365 bp
  • C to A, chromosome 12 at 19,490,620 bp
  • T to C, chromosome 12 at 71,061,525 bp
  • A to G, chromosome 12 at 73,112,883 bp
  • T to G, chromosome 12 at 76,610,815 bp
  • A to T, chromosome 12 at 81,419,822 bp
  • A to T, chromosome 12 at 87,464,724 bp
  • A to G, chromosome 12 at 104,411,277 bp
  • T to C, chromosome 13 at 70,753,226 bp
  • A to T, chromosome 14 at 31,269,817 bp
  • T to A, chromosome 14 at 52,410,361 bp
  • G to T, chromosome 16 at 34,212,272 bp
  • C to T, chromosome 16 at 91,928,906 bp
  • T to C, chromosome 16 at 97,743,957 bp
  • T to A, chromosome 17 at 15,431,635 bp
  • T to C, chromosome 17 at 25,744,517 bp
  • C to A, chromosome 18 at 34,269,259 bp
  • A to G, chromosome 18 at 37,335,892 bp
  • T to C, chromosome 18 at 63,740,017 bp
  • G to C, chromosome 19 at 13,028,047 bp
  • A to G, chromosome 19 at 59,345,413 bp
  • C to A, chromosome X at 134,691,025 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3411 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040629-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.