Strain Name:
C57BL/6J-MtgxR3425Btlr/Mmmh
Stock Number:
040643-MU
Citation ID:
RRID:MMRRC_040643-MU
Other Names:
R3425 (G1), C57BL/6J-MtgxR3425Btlr
Major Collection:

Gene Information

Upp1
Name: uridine phosphorylase 1
Synonyms: UdRPase, UPase, Up
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 22271
Homologene: 2524
Mapt
Name: microtubule-associated protein tau
Synonyms: Mtapt, Tau
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 17762
HGNC: HGNC:6893
Homologene: 74962
Zranb3
Name: zinc finger, RAN-binding domain containing 3
Synonyms: 4933425L19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226409
Homologene: 32770
Kat7
Name: K(lysine) acetyltransferase 7
Synonyms: Hbo1, Hboa, Myst2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217127
Homologene: 5134
Myo1d
Name: myosin ID
Synonyms: 9930104H07Rik, D11Ertd9e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 338367
HGNC: HGNC:7598
Homologene: 45576
Ap5m1
Name: adaptor-related protein complex 5, mu 1 subunit
Synonyms: 4932432K03Rik, Mudeng
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 74385
VEGA: 14
Homologene: 10081
Hspg2
Name: perlecan (heparan sulfate proteoglycan 2)
Synonyms: per, Pcn, Plc, perlecan
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 15530
HGNC: HGNC:5273
Homologene: 68473
Ddx11
Name: DEAD/H box helicase 11
Synonyms: CHLR1, KRG2, CHL1, 4732462I11Rik, essa15a, DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 320209
VEGA: 17
HGNC: HGNC:2736
Homologene: 68973
Ptpn4
Name: protein tyrosine phosphatase, non-receptor type 4
Synonyms: hPTP-MEG, protein tyrosine phosphatase, non-receptor type 4 (megakaryocyte), TEP/mPTPMEG, testis-enriched phosphatase, TEP, PTPMEG
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 19258
HGNC: HGNC:9656
Homologene: 2120
Sez6l
Name: seizure related 6 homolog like
Synonyms: Acig1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 56747
Homologene: 10895
Il16
Name: interleukin 16
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 16170
HGNC: HGNC:5980
Homologene: 18157
H2-T22
Name: histocompatibility 2, T region locus 22
Synonyms: H-2T22, H-2T17, H2-T17
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 15039
Homologene: 79525
Kcnk2
Name: potassium channel, subfamily K, member 2
Synonyms: TREK-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 16526
HGNC: HGNC:6277
Homologene: 7794
Ism2
Name: isthmin 2
Synonyms: LOC217738, Thsd3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 217738
Homologene: 83509
Cyp2c70
Name: cytochrome P450, family 2, subfamily c, polypeptide 70
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 226105
Homologene: 120027
Cdc16
Name: CDC16 cell division cycle 16
Synonyms: 2810431D22Rik, 2700071J12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 69957
HGNC: HGNC:1720
Homologene: 2899
Csmd3
Name: CUB and Sushi multiple domains 3
Synonyms: 4930500N14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 239420
Homologene: 65982
Ros1
Name: Ros1 proto-oncogene
Synonyms: Ros-1, c-ros
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 19886
Homologene: 2207
Olfr1453
Name: olfactory receptor 1453
Synonyms: GA_x6K02T2RE5P-3357666-3356743, MOR202-6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 258695
Homologene: 120159
Tmprss15
Name: transmembrane protease, serine 15
Synonyms: enteropeptidase, enterokinase, A130097D21Rik, Prss7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 19146
HGNC: HGNC:9490
Homologene: 2075
Mypn
Name: myopalladin
Synonyms: 1110056A04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 68802
VEGA: 10
Homologene: 23778
Olfr1098
Name: olfactory receptor 1098
Synonyms: GA_x6K02T2Q125-48410458-48409511, MOR206-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258842
Homologene: 37005
Atp8b3
Name: ATPase, class I, type 8B, member 3
Synonyms: SAPLT, 1700042F02Rik, 1700056N23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 67331
VEGA: 10
Homologene: 19034
Scel
Name: sciellin
Synonyms: 9230114I02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 64929
VEGA: 14
Homologene: 2850
Slco4c1
Name: solute carrier organic anion transporter family, member 4C1
Synonyms: SLC21A20, PRO2176, OATP4C1, OATP-M1, OATP-H, C330017E21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 227394
Homologene: 62654
AC091683.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Slc18b1
Name: solute carrier family 18, subfamily B, member 1
Synonyms: 1110021L09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 76306
Homologene: 33473
Zp1
Name: zona pellucida glycoprotein 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 22786
Homologene: 33483
Slc36a3
Name: solute carrier family 36 (proton/amino acid symporter), member 3
Synonyms: tramdorin2, PAT3, TRAMD2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 215332
Homologene: 76175
Meltf
Name: melanotransferrin
Synonyms: MTf, CD228, melanotransferrin, Mfi2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 30060
VEGA: 16
HGNC: HGNC:7037
Homologene: 4335
Tmem181a
Name: transmembrane protein 181A
Synonyms: 5930418K15Rik, Gpr178, C76977, Tmem181
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 77106
Homologene: 44787
Hmgcs1
Name: 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1
Synonyms: B130032C06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 208715
VEGA: 13
HGNC: HGNC:5007
Homologene: 1609
Klf15
Name: Kruppel-like factor 15
Synonyms: KKLF, CKLF, 1810013I09Rik, hlb444
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 66277
Homologene: 8553
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 96,841,251 bp
  • T to C, chromosome 1 at 119,707,830 bp
  • A to G, chromosome 1 at 127,955,074 bp
  • C to T, chromosome 1 at 189,339,772 bp
  • C to T, chromosome 2 at 86,922,606 bp
  • C to T, chromosome 4 at 137,511,380 bp
  • T to C, chromosome 5 at 112,426,749 bp
  • T to C, chromosome 6 at 90,466,820 bp
  • T to C, chromosome 7 at 83,644,040 bp
  • A to G, chromosome 8 at 13,763,808 bp
  • T to G, chromosome 10 at 23,822,976 bp
  • C to A, chromosome 10 at 52,128,416 bp
  • T to C, chromosome 10 at 63,118,417 bp
  • T to C, chromosome 10 at 80,536,347 bp
  • T to C, chromosome 11 at 9,125,700 bp
  • G to T, chromosome 11 at 55,142,781 bp
  • T to C, chromosome 11 at 80,601,638 bp
  • T to C, chromosome 11 at 95,303,165 bp
  • C to T, chromosome 11 at 104,298,722 bp
  • T to C, chromosome 12 at 87,287,097 bp
  • T to A, chromosome 13 at 49,901,756 bp
  • C to A, chromosome 13 at 119,705,132 bp
  • A to G, chromosome 14 at 49,073,683 bp
  • T to G, chromosome 14 at 103,608,106 bp
  • A to T, chromosome 15 at 47,847,252 bp
  • C to T, chromosome 16 at 31,896,525 bp
  • A to T, chromosome 16 at 79,003,433 bp
  • T to A, chromosome 17 at 6,295,786 bp
  • A to C, chromosome 17 at 36,041,580 bp
  • T to C, chromosome 17 at 66,139,439 bp
  • G to A, chromosome 19 at 10,918,592 bp
  • G to C, chromosome 19 at 13,028,047 bp
  • G to T, chromosome 19 at 40,184,024 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3425 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040643-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.