Strain Name:
C57BL/6J-MtgxR3427Btlr/Mmmh
Stock Number:
040645-MU
Citation ID:
RRID:MMRRC_040645-MU
Other Names:
R3427 (G1), C57BL/6J-MtgxR3427Btlr
Major Collection:

Gene Information

Crhr1
Name: corticotropin releasing hormone receptor 1
Synonyms: CRFR1, CRF-R1alpha, CRF 1 receptor, CRF1R
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 12921
HGNC: HGNC:2357
Homologene: 20920
Nudc
Name: nudC nuclear distribution protein
Synonyms: NudC, Silg92
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 18221
HGNC: HGNC:8045
Homologene: 4812
Ctbp2
Name: C-terminal binding protein 2
Synonyms: D7Ertd45e, Ribeye, Gtrgeo6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 13017
HGNC: HGNC:2495
Homologene: 75187
Fiz1
Name: Flt3 interacting zinc finger protein 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 23877
Homologene: 22663
Sec24d
Name: Sec24 related gene family, member D (S. cerevisiae)
Synonyms: 2310020L09Rik, LOC383951
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 69608
Homologene: 40986
Rsf1
Name: remodeling and spacing factor 1
Synonyms: p325, XAP8, Hbxap, C030033M12Rik, 4832420A03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233532
Homologene: 41142
Rttn
Name: rotatin
Synonyms: 4921538A15Rik, C530033I08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 246102
VEGA: 18
Homologene: 65275
Ep300
Name: E1A binding protein p300
Synonyms: p300, KAT3B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 328572
HGNC: HGNC:3373
Homologene: 1094
Rbm26
Name: RNA binding motif protein 26
Synonyms: Pro1777, C230097K14Rik, 1700009P03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 74213
Homologene: 41468
Exosc2
Name: exosome component 2
Synonyms: Rrp4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 227715
Homologene: 6095
Cul5
Name: cullin 5
Synonyms: 4921514I20Rik, C330021I08Rik, C030032G03Rik, VACM-1, 8430423K24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 75717
HGNC: HGNC:2556
Homologene: 2597
Atp13a3
Name: ATPase type 13A3
Synonyms: LOC224088, LOC224087, LOC385637
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 224088
Homologene: 23455
Chd6
Name: chromodomain helicase DNA binding protein 6
Synonyms: 6330406J24Rik, 5430439G14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 71389
Homologene: 32772
Nup54
Name: nucleoporin 54
Synonyms: 3110079L04Rik, 54kDa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 269113
Homologene: 41169
Trpc1
Name: transient receptor potential cation channel, subfamily C, member 1
Synonyms: Trrp1, Trp1, Mtrp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 22063
Homologene: 2478
Bsph1
Name: binder of sperm protein homolog 1
Synonyms: LOC330470
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 330470
Homologene: 86818
Tns2
Name: tensin 2
Synonyms: nep, nph, Tenc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 209039
VEGA: 15
Homologene: 37077
Gm5422
Name: predicted pseudogene 5422
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 432448
VEGA: 10
Unc80
Name: unc-80, NALCN activator
Synonyms: C230061B10Rik, C030018G13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 329178
Homologene: 122243
Sycp1
Name: synaptonemal complex protein 1
Synonyms: SCP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 20957
Homologene: 2389
Obscn
Name: obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF
Synonyms: LOC380698, OTTMUSG00000005786
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 380698
Homologene: 70869
Stab1
Name: stabilin 1
Synonyms: MS-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 192187
Homologene: 9035
Slc22a5
Name: solute carrier family 22 (organic cation transporter), member 5
Synonyms: Octn2, Lstpl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 20520
Homologene: 68295
Vmn2r63
Name: vomeronasal 2, receptor 63
Synonyms: EG435975
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 435975
Homologene: 104832
Trpv3
Name: transient receptor potential cation channel, subfamily V, member 3
Synonyms: 1110036I10Rik, VRL3, Nh
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 246788
Homologene: 17040
Cps1
Name: carbamoyl-phosphate synthetase 1
Synonyms: CPS, CPSase I, 4732433M03Rik, D1Ucla3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 227231
HGNC: HGNC:2323
Homologene: 68208
Igsf9b
Name: immunoglobulin superfamily, member 9B
Synonyms: LOC235086, AI414108
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235086
Homologene: 19472
Actl11
Name: actin-like 11
Synonyms: 4921517D21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 67722
Homologene: 69412
Strip1
Name: striatin interacting protein 1
Synonyms: 6530401O14Rik, 6330569M22Rik, Fam40a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229707
Homologene: 35064
Kcnh1
Name: potassium voltage-gated channel, subfamily H (eag-related), member 1
Synonyms: ether a go-go, Eag1, Kv10.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 16510
HGNC: HGNC:6250
Homologene: 68242
Epb42
Name: erythrocyte membrane protein band 4.2
Synonyms: Epb4.2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 13828
HGNC: HGNC:3381
Homologene: 93
Igtp
Name: interferon gamma induced GTPase
Synonyms: Irgm3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 16145
Homologene: 23118
Plscr4
Name: phospholipid scramblase 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235527
Homologene: 23217
Rps6ka4
Name: ribosomal protein S6 kinase, polypeptide 4
Synonyms: MSK2, 1110069D02Rik, 90kDa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 56613
VEGA: 19
Homologene: 69288
Angel1
Name: angel homolog 1
Synonyms: 1110030H02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 68737
Homologene: 32251
Asb18
Name: ankyrin repeat and SOCS box-containing 18
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 208372
Homologene: 16382
Olfr811
Name: olfactory receptor 811
Synonyms: GA_x6K02T2PULF-11481207-11480248, MOR110-6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 258545
Homologene: 133725
Plekhb1
Name: pleckstrin homology domain containing, family B (evectins) member 1
Synonyms: PHR1, Phret1, evt-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 27276
Homologene: 8449
Tmem181a
Name: transmembrane protein 181A
Synonyms: 5930418K15Rik, Gpr178, C76977, Tmem181
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 77106
Homologene: 44787
Tdrkh
Name: tudor and KH domain containing protein
Synonyms: Tdrd2, 2700091C21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 72634
Homologene: 4999
Dctn3
Name: dynactin 3
Synonyms: p24
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 53598
HGNC: HGNC:2713
Homologene: 5233
Crygs
Name: crystallin, gamma S
Synonyms: Opj
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 12970
VEGA: 16
HGNC: HGNC:2417
Homologene: 40695
Otub2
Name: OTU domain, ubiquitin aldehyde binding 2
Synonyms: 2010015L18Rik, OTU2, OTB2, 4930586I02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 68149
Homologene: 57056
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 66,639,305 bp
  • T to A, chromosome 1 at 67,174,494 bp
  • C to T, chromosome 1 at 89,968,593 bp
  • T to C, chromosome 1 at 192,241,930 bp
  • T to C, chromosome 2 at 31,679,888 bp
  • A to T, chromosome 2 at 121,030,039 bp
  • G to T, chromosome 2 at 160,990,255 bp
  • T to C, chromosome 3 at 94,423,807 bp
  • A to T, chromosome 3 at 102,876,350 bp
  • T to C, chromosome 3 at 107,616,822 bp
  • C to T, chromosome 3 at 123,343,923 bp
  • T to C, chromosome 4 at 41,719,858 bp
  • C to T, chromosome 4 at 133,534,257 bp
  • A to G, chromosome 5 at 92,431,027 bp
  • A to G, chromosome 7 at 5,012,709 bp
  • T to C, chromosome 7 at 13,472,243 bp
  • A to G, chromosome 7 at 42,926,982 bp
  • GCG to GCGACGGCGTCG, chromosome 7 at 97,579,907 bp
  • T to C, chromosome 7 at 100,645,650 bp
  • A to T, chromosome 7 at 132,991,592 bp
  • T to C, chromosome 9 at 27,334,577 bp
  • T to C, chromosome 9 at 53,617,890 bp
  • T to C, chromosome 9 at 92,488,744 bp
  • C to T, chromosome 9 at 95,732,196 bp
  • T to A, chromosome 9 at 107,929,770 bp
  • A to G, chromosome 10 at 31,248,846 bp
  • T to C, chromosome 10 at 129,801,851 bp
  • A to G, chromosome 11 at 53,869,326 bp
  • G to A, chromosome 11 at 58,206,593 bp
  • C to G, chromosome 11 at 59,067,930 bp
  • A to T, chromosome 11 at 73,285,941 bp
  • A to G, chromosome 11 at 104,173,593 bp
  • A to T, chromosome 12 at 86,721,392 bp
  • T to C, chromosome 12 at 103,403,438 bp
  • G to A, chromosome 14 at 31,140,143 bp
  • A to G, chromosome 14 at 105,131,532 bp
  • A to G, chromosome 15 at 81,601,279 bp
  • C to T, chromosome 15 at 102,108,934 bp
  • C to T, chromosome 16 at 22,805,551 bp
  • T to C, chromosome 16 at 30,344,593 bp
  • T to A, chromosome 17 at 6,295,786 bp
  • G to A, chromosome 18 at 89,095,651 bp
  • C to A, chromosome 19 at 6,837,755 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3427 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040645-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.