Strain Name:
C57BL/6J-MtgxR3431Btlr/Mmmh
Stock Number:
040649-MU
Citation ID:
RRID:MMRRC_040649-MU
Other Names:
R3431 (G1), C57BL/6J-MtgxR3431Btlr
Major Collection:

Gene Information

Evl
Name: Ena-vasodilator stimulated phosphoprotein
Synonyms: b2b2600Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 14026
VEGA: 12
Homologene: 56752
Knl1
Name: kinetochore scaffold 1
Synonyms: 5730505K17Rik, Casc5, 2310043D08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 76464
Homologene: 44890
Chd4
Name: chromodomain helicase DNA binding protein 4
Synonyms: 9530019N15Rik, Mi-2beta, D6Ertd380e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 107932
HGNC: HGNC:1919
Homologene: 68175
Rttn
Name: rotatin
Synonyms: C530033I08Rik, 4921538A15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 246102
VEGA: 18
Homologene: 65275
Cbl
Name: Casitas B-lineage lymphoma
Synonyms: c-Cbl, 4732447J05Rik, Cbl-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 12402
HGNC: HGNC:1541
Homologene: 3802
Usp34
Name: ubiquitin specific peptidase 34
Synonyms: A530081C03Rik, Murr2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 17847
Homologene: 40978
Mcm2
Name: minichromosome maintenance complex component 2
Synonyms: CDCL1, Mcmd2, BM28
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 17216
HGNC: HGNC:6944
Homologene: 3325
Efs
Name: embryonal Fyn-associated substrate
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 13644
VEGA: 14
Homologene: 4284
Apob
Name: apolipoprotein B
Synonyms: apob-100, apob-48
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 238055
HGNC: HGNC:603
Homologene: 328
Erc1
Name: ELKS/RAB6-interacting/CAST family member 1
Synonyms: B430107L16Rik, 9630025C19Rik, Elks1, RAB6IP2A, RAB6IP2B, 5033405M01Rik, Rab6ip2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 111173
Homologene: 14229
Gm4924
Name: predicted gene 4924
Synonyms: mIF1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 237412
Tbc1d19
Name: TBC1 domain family, member 19
Synonyms: 2810453K03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 67249
Homologene: 10130
Uggt1
Name: UDP-glucose glycoprotein glucosyltransferase 1
Synonyms: A930007H10Rik, Ugcgl1, 0910001L17Rik, C820010P03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 320011
Homologene: 10586
Calcb
Name: calcitonin-related polypeptide, beta
Synonyms: CGRP2, Calc2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 116903
HGNC: HGNC:1438
Homologene: 137656
Clec4a2
Name: C-type lectin domain family 4, member a2
Synonyms: dendritic cell immunoreceptor, Clecsf6, Dcir1, DCIR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 26888
Homologene: 49291
Mthfd2
Name: methylenetetrahydrofolate dehydrogenase (NAD+ dependent), methenyltetrahydrofolate cyclohydrolase
Synonyms: NMDMC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 17768
HGNC: HGNC:7434
Homologene: 21321
Dnah5
Name: dynein, axonemal, heavy chain 5
Synonyms: b2b3491Clo, b2b1565Clo, Mdnah5, b2b1154Clo, b2b1537Clo, b2b1134Clo, Dnahc5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 110082
HGNC: HGNC:2950
Homologene: 1048
Taf5
Name: TATA-box binding protein associated factor 5
Synonyms: 6330528C20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 226182
VEGA: 19
Homologene: 5064
Fsip2
Name: fibrous sheath-interacting protein 2
Synonyms: OTTMUSG00000013335
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 241516
Homologene: 110349
Ryr3
Name: ryanodine receptor 3
Synonyms: calcium release channel isoform 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 20192
Homologene: 68151
Tcl1b3
Name: T cell leukemia/lymphoma 1B, 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 27378
VEGA: 12
Homologene: 83274
Adamts3
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 3
Synonyms: 6330442E02Rik, 1100001H14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 330119
HGNC: HGNC:219
Homologene: 8596
Npas3
Name: neuronal PAS domain protein 3
Synonyms: 4930423H22Rik, bHLHe12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 27386
VEGA: 12
Homologene: 8461
Mfsd13a
Name: major facilitator superfamily domain containing 13a
Synonyms: 4930538D17Rik, Tmem180, 4930449A08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 75146
Homologene: 12592
Tulp4
Name: tubby like protein 4
Synonyms: 2210038L17Rik, 1110057P05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 68842
Homologene: 32467
Cyp2c39
Name: cytochrome P450, family 2, subfamily c, polypeptide 39
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 13098
HGNC: HGNC:2622
Homologene: 117948
Hhipl1
Name: hedgehog interacting protein-like 1
Synonyms: 1600002O04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 214305
VEGA: 12
Homologene: 81985
Tssk4
Name: testis-specific serine kinase 4
Synonyms: 1700020B19Rik, 4933424F08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 71099
VEGA: 14
Homologene: 57078
Nr1h3
Name: nuclear receptor subfamily 1, group H, member 3
Synonyms: Unr1, LXR alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 22259
HGNC: HGNC:7966
Homologene: 21165
H60c
Name: histocompatibility 60c
Synonyms: 4632413I24Rik, Gm9491
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 670558
VEGA: 10
Homologene: 120804
Tmem232
Name: transmembrane protein 232
Synonyms: LOC381107, E130009J12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 381107
VEGA: 17
Homologene: 54378
Dhrs7
Name: dehydrogenase/reductase (SDR family) member 7
Synonyms: retSDR4, 2310016E22Rik, retDSR4, 5730564L20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 66375
VEGA: 12
Homologene: 9350
AC147549.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: Y
Alteration at locus: Chemically Induced
Rap2a
Name: RAS related protein 2a
Synonyms: 5830461H18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 76108
HGNC: HGNC:9861
Homologene: 10875
Mmp21
Name: matrix metallopeptidase 21
Synonyms: b2b873Clo, b2b2458Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 214766
Homologene: 17519
Bahd1
Name: bromo adjacent homology domain containing 1
Synonyms: LOC228536
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 228536
Homologene: 8976
Fbxo16
Name: F-box protein 16
Synonyms: Fbx16
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 50759
VEGA: 14
Homologene: 9273
Crb2
Name: crumbs family member 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 241324
Homologene: 45474
Hbq1a
Name: hemoglobin, theta 1A
Synonyms: Hbq1, LOC380691, A230090E05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 216635
HGNC: HGNC:4833
Mup4
Name: major urinary protein 4
Synonyms: Mup-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 17843
Homologene: 74304
Acox3
Name: acyl-Coenzyme A oxidase 3, pristanoyl
Synonyms: pristanoyl-CoA oxidase, PCOX, EST-s59
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 80911
HGNC: HGNC:121
Homologene: 37792
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 36,210,059 bp
  • T to A, chromosome 2 at 37,792,217 bp
  • A to G, chromosome 2 at 82,992,010 bp
  • T to C, chromosome 2 at 91,191,860 bp
  • A to T, chromosome 2 at 112,656,531 bp
  • G to A, chromosome 2 at 118,922,523 bp
  • A to T, chromosome 2 at 119,062,362 bp
  • T to G, chromosome 4 at 59,959,192 bp
  • A to G, chromosome 5 at 35,589,216 bp
  • T to C, chromosome 5 at 53,848,206 bp
  • A to G, chromosome 5 at 89,707,453 bp
  • T to C, chromosome 6 at 83,311,348 bp
  • G to A, chromosome 6 at 88,893,008 bp
  • C to A, chromosome 6 at 119,783,475 bp
  • A to T, chromosome 6 at 123,139,411 bp
  • A to G, chromosome 6 at 125,120,560 bp
  • A to T, chromosome 7 at 114,719,829 bp
  • T to C, chromosome 7 at 133,678,750 bp
  • T to C, chromosome 9 at 44,151,446 bp
  • G to T, chromosome 10 at 3,260,382 bp
  • T to A, chromosome 10 at 82,379,030 bp
  • T to C, chromosome 11 at 23,370,466 bp
  • T to G, chromosome 11 at 32,300,715 bp
  • T to G, chromosome 12 at 8,010,778 bp
  • A to T, chromosome 12 at 54,069,049 bp
  • A to T, chromosome 12 at 72,664,727 bp
  • T to C, chromosome 12 at 105,193,407 bp
  • A to G, chromosome 12 at 108,311,689 bp
  • T to C, chromosome 12 at 108,648,308 bp
  • C to T, chromosome 14 at 54,920,224 bp
  • A to G, chromosome 14 at 55,651,695 bp
  • T to C, chromosome 14 at 65,293,784 bp
  • G to T, chromosome 14 at 120,503,758 bp
  • A to G, chromosome 15 at 28,295,267 bp
  • C to A, chromosome 17 at 6,206,964 bp
  • A to T, chromosome 17 at 65,265,302 bp
  • A to G, chromosome 18 at 89,095,571 bp
  • A to G, chromosome 19 at 39,536,862 bp
  • C to T, chromosome 19 at 46,371,992 bp
  • A to C, chromosome 19 at 47,075,833 bp
  • A to T, chromosome Y at 10,641,956 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3431 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040649-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.