Strain Name:
C57BL/6J-MtgxR3440Btlr/Mmmh
Stock Number:
040658-MU
Citation ID:
RRID:MMRRC_040658-MU
Other Names:
R3440 (G1), C57BL/6J-MtgxR3440Btlr
Major Collection:

Gene Information

Dhrs3
Name: dehydrogenase/reductase (SDR family) member 3
Synonyms: retSDR1, Rsdr1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 20148
Homologene: 20994
Ano6
Name: anoctamin 6
Synonyms: F730003B03Rik, 2900059G15Rik, Tmem16f
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 105722
VEGA: 15
Homologene: 27888
Zfat
Name: zinc finger and AT hook domain containing
Synonyms: Zfat1, Zfp406, LOC380993
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 380993
Homologene: 16829
4932438A13Rik
Name: RIKEN cDNA 4932438A13 gene
Synonyms: FSA, Tweek
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229227
Homologene: 52105
Pgr
Name: progesterone receptor
Synonyms: 9930019P03Rik, PR-A, PR-B, NR3C3, ENSMUSG00000074510, PR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 18667
HGNC: HGNC:8910
Homologene: 713
Lef1
Name: lymphoid enhancer binding factor 1
Synonyms: lymphoid enhancer factor 1, Lef-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 16842
HGNC: HGNC:6551
Homologene: 7813
Grin2c
Name: glutamate receptor, ionotropic, NMDA2C (epsilon 3)
Synonyms: NMDAR2C, GluRepsilon3, NR2C
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 14813
HGNC: HGNC:4587
Homologene: 647
Tenm4
Name: teneurin transmembrane protein 4
Synonyms: ELM2, Doc4, l(7)-3Rn, Ten-m4, Odz4, l7Rn3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 23966
Homologene: 8034
Chn2
Name: chimerin 2
Synonyms: 4930557O16Rik, 1700026N20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 69993
HGNC: HGNC:1944
Homologene: 31213
Cp
Name: ceruloplasmin
Synonyms: D3Ertd555e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 12870
HGNC: HGNC:2295
Homologene: 75
Kif1a
Name: kinesin family member 1A
Synonyms: C630002N23Rik, Kns1, N-3 kinesin, LOC381283, ATSV
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 16560
HGNC: HGNC:888
Homologene: 99729
Adamts3
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 3
Synonyms: 1100001H14Rik, 6330442E02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 330119
HGNC: HGNC:219
Homologene: 8596
Grik3
Name: glutamate receptor, ionotropic, kainate 3
Synonyms: Glur7, Glur-7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 14807
HGNC: HGNC:4581
Homologene: 73901
Elapor2
Name: endosome-lysosome associated apoptosis and autophagy regulator family member 2
Synonyms: 9330182L06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231014
Homologene: 27396
AC104834.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Myom1
Name: myomesin 1
Synonyms: D430047A17Rik, skelemin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 17929
VEGA: 17
HGNC: HGNC:7613
Homologene: 31196
Abtb2
Name: ankyrin repeat and BTB (POZ) domain containing 2
Synonyms: BPOZ-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 99382
Homologene: 15904
Fpr1
Name: formyl peptide receptor 1
Synonyms: FPR, fMLF-R
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 14293
HGNC: HGNC:3826
Homologene: 20466
Tchh
Name: trichohyalin
Synonyms: Thh, AHF
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 99681
Homologene: 136273
Psg27
Name: pregnancy-specific glycoprotein 27
Synonyms: EG545925, cea15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 545925
Homologene: 110989
Adam3
Name: a disintegrin and metallopeptidase domain 3 (cyritestin)
Synonyms: tMDC, Taz83, Cyrn1, Taz83, ADAM3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 11497
HGNC: HGNC:209
Homologene: 69052
Arhgap45
Name: Rho GTPase activating protein 45
Synonyms: 6330406L22Rik, Hmha1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 70719
Homologene: 69120
Clrn3
Name: clarin 3
Synonyms: Tmem12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 212070
Homologene: 17544
Olfr420
Name: olfactory receptor 420
Synonyms: GA_x6K02T2P20D-20995211-20994246, MOR105-10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 258302
Homologene: 17185
Agmo
Name: alkylglycerol monooxygenase
Synonyms: Tmem195, A530016O06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 319660
Homologene: 45620
H2-Eb1
Name: histocompatibility 2, class II antigen E beta
Synonyms: Ia4, Ia-4, H-2Eb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 14969
HGNC: HGNC:4953
Homologene: 101524
Selp
Name: selectin, platelet
Synonyms: CD62P, P-selectin, Grmp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 20344
Homologene: 2260
Cavin2
Name: caveolae associated 2
Synonyms: Sdpr, cavin 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 20324
Homologene: 3422
Gtf3c6
Name: general transcription factor IIIC, polypeptide 6, alpha
Synonyms: 2410016F19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 67371
VEGA: 10
Homologene: 12121
Rab36
Name: RAB36, member RAS oncogene family
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 76877
HGNC: HGNC:9775
Homologene: 3610
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 51,301,406 bp
  • C to T, chromosome 1 at 93,036,853 bp
  • T to C, chromosome 1 at 164,123,775 bp
  • A to T, chromosome 1 at 174,159,180 bp
  • C to A, chromosome 2 at 103,567,232 bp
  • A to T, chromosome 3 at 19,974,957 bp
  • C to T, chromosome 3 at 37,041,912 bp
  • A to T, chromosome 3 at 93,445,107 bp
  • A to G, chromosome 3 at 131,184,758 bp
  • C to A, chromosome 4 at 125,693,970 bp
  • T to A, chromosome 4 at 125,693,971 bp
  • T to A, chromosome 4 at 144,920,058 bp
  • T to A, chromosome 5 at 9,428,548 bp
  • A to G, chromosome 5 at 89,698,193 bp
  • A to G, chromosome 6 at 54,267,267 bp
  • T to A, chromosome 7 at 18,567,060 bp
  • A to T, chromosome 7 at 96,553,516 bp
  • A to C, chromosome 7 at 135,514,125 bp
  • T to C, chromosome 8 at 24,680,743 bp
  • A to T, chromosome 9 at 8,922,629 bp
  • T to A, chromosome 10 at 40,251,173 bp
  • G to A, chromosome 10 at 75,044,496 bp
  • G to T, chromosome 10 at 80,021,468 bp
  • T to A, chromosome 11 at 115,250,643 bp
  • C to T, chromosome 12 at 37,243,800 bp
  • A to T, chromosome 15 at 68,084,553 bp
  • T to C, chromosome 15 at 95,967,721 bp
  • A to G, chromosome 15 at 101,053,123 bp
  • C to T, chromosome 17 at 17,877,158 bp
  • A to G, chromosome 17 at 34,309,681 bp
  • A to G, chromosome 17 at 71,045,663 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3440 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040658-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.