Strain Name:
C57BL/6J-MtgxR3440Btlr/Mmmh
Stock Number:
040658-MU
Citation ID:
RRID:MMRRC_040658-MU
Other Names:
R3440 (G1), C57BL/6J-MtgxR3440Btlr
Major Collection:

Strain Information

Dhrs3
Name: dehydrogenase/reductase 3
Synonyms: retSDR1, Rsdr1, dehydrogenase/reductase (SDR family) member 3
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 20148
Homologene: 20994
Ano6
Name: anoctamin 6
Synonyms: 2900059G15Rik, F730003B03Rik, Tmem16f
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 105722
VEGA: 15
Homologene: 27888
Zfat
Name: zinc finger and AT hook domain containing
Synonyms: LOC380993, Zfp406, Zfat1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 380993
Homologene: 16829
Bltp1
Name: bridge-like lipid transfer protein family member 1
Synonyms: Tweek, FSA, 4932438A13Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229227
Homologene: 52105
Pgr
Name: progesterone receptor
Synonyms: PR, NR3C3, 9930019P03Rik, PR-B, PR-A, ENSMUSG00000074510
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 18667
HGNC: HGNC:8910
Homologene: 713
Lef1
Name: lymphoid enhancer binding factor 1
Synonyms: lymphoid enhancer factor 1, Lef-1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 16842
HGNC: HGNC:6551
Homologene: 7813
Grin2c
Name: glutamate receptor, ionotropic, NMDA2C (epsilon 3)
Synonyms: NMDAR2C, NR2C, GluRepsilon3
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14813
HGNC: HGNC:4587
Homologene: 647
Tenm4
Name: teneurin transmembrane protein 4
Synonyms: l(7)-3Rn, Ten-m4, Doc4, l7Rn3, ELM2, Odz4
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 23966
Homologene: 8034
Chn2
Name: chimerin 2
Synonyms: 1700026N20Rik, 4930557O16Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 69993
HGNC: HGNC:1944
Homologene: 31213
Cp
Name: ceruloplasmin
Synonyms: D3Ertd555e
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 12870
HGNC: HGNC:2295
Homologene: 75
Kif1a
Name: kinesin family member 1A
Synonyms: Kns1, ATSV, N-3 kinesin, LOC381283, C630002N23Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16560
HGNC: HGNC:888
Homologene: 99729
Adamts3
Name: ADAM metallopeptidase with thrombospondin type 1 motif 3
Synonyms: 6330442E02Rik, 1100001H14Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 330119
HGNC: HGNC:219
Homologene: 8596
Grik3
Name: glutamate receptor, ionotropic, kainate 3
Synonyms: Glur-7, Glur7
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 14807
HGNC: HGNC:4581
Homologene: 73901
Elapor2
Name: endosome-lysosome associated apoptosis and autophagy regulator family member 2
Synonyms: 9330182L06Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231014
Homologene: 27396
AC104834.1
Name:
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Myom1
Name: myomesin 1
Synonyms: skelemin, D430047A17Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 17929
VEGA: 17
HGNC: HGNC:7613
Homologene: 31196
Abtb2
Name: ankyrin repeat and BTB domain containing 2
Synonyms: BPOZ-2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 99382
Homologene: 15904
Fpr1
Name: formyl peptide receptor 1
Synonyms: FPR, fMLF-R
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 14293
HGNC: HGNC:3826
Homologene: 20466
Tchh
Name: trichohyalin
Synonyms: Thh, AHF
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 99681
Homologene: 136273
Psg27
Name: pregnancy-specific beta-1-glycoprotein 27
Synonyms: cea15, EG545925
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 545925
Homologene: 110989
Adam3
Name: ADAM metallopeptidase domain 3
Synonyms: Taz83, tMDC, Cyrn1, ADAM3, Taz83
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 11497
HGNC: HGNC:209
Homologene: 69052
Arhgap45
Name: Rho GTPase activating protein 45
Synonyms: 6330406L22Rik, Hmha1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 70719
Homologene: 69120
Clrn3
Name: clarin 3
Synonyms: Tmem12
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 212070
Homologene: 17544
Or6k2
Name: olfactory receptor family 6 subfamily K member 2
Synonyms: GA_x6K02T2P20D-20995211-20994246, MOR105-10, Olfr420
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 258302
Homologene: 17185
Agmo
Name: alkylglycerol monooxygenase
Synonyms: A530016O06Rik, Tmem195
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 319660
Homologene: 45620
H2-Eb1
Name: histocompatibility 2, class II antigen E beta
Synonyms: H-2Eb, Ia-4, Ia4
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 14969
Homologene: 101524
Selp
Name: selectin, platelet
Synonyms: CD62P, P-selectin, Grmp
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 20344
Homologene: 2260
Cavin2
Name: caveolae associated 2
Synonyms: cavin 2, Sdpr
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 20324
Homologene: 3422
Gtf3c6
Name: general transcription factor IIIC, polypeptide 6, alpha
Synonyms: 2410016F19Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 67371
VEGA: 10
Homologene: 12121
Rab36
Name: RAB36, member RAS oncogene family
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 76877
HGNC: HGNC:9775
Homologene: 3610
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 51,301,406 bp
  • C to T, chromosome 1 at 93,036,853 bp
  • T to C, chromosome 1 at 164,123,775 bp
  • A to T, chromosome 1 at 174,159,180 bp
  • C to A, chromosome 2 at 103,567,232 bp
  • A to T, chromosome 3 at 19,974,957 bp
  • C to T, chromosome 3 at 37,041,912 bp
  • A to T, chromosome 3 at 93,445,107 bp
  • A to G, chromosome 3 at 131,184,758 bp
  • C to A, chromosome 4 at 125,693,970 bp
  • T to A, chromosome 4 at 125,693,971 bp
  • T to A, chromosome 4 at 144,920,058 bp
  • T to A, chromosome 5 at 9,428,548 bp
  • A to G, chromosome 5 at 89,698,193 bp
  • A to G, chromosome 6 at 54,267,267 bp
  • T to A, chromosome 7 at 18,567,060 bp
  • A to T, chromosome 7 at 96,553,516 bp
  • A to C, chromosome 7 at 135,514,125 bp
  • T to C, chromosome 8 at 24,680,743 bp
  • A to T, chromosome 9 at 8,922,629 bp
  • T to A, chromosome 10 at 40,251,173 bp
  • G to A, chromosome 10 at 75,044,496 bp
  • G to T, chromosome 10 at 80,021,468 bp
  • T to A, chromosome 11 at 115,250,643 bp
  • C to T, chromosome 12 at 37,243,800 bp
  • A to T, chromosome 15 at 68,084,553 bp
  • T to C, chromosome 15 at 95,967,721 bp
  • A to G, chromosome 15 at 101,053,123 bp
  • C to T, chromosome 17 at 17,877,158 bp
  • A to G, chromosome 17 at 34,309,681 bp
  • A to G, chromosome 17 at 71,045,663 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3440 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040658-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.