Strain Name:
C57BL/6J-MtgxR3612Btlr/Mmmh
Stock Number:
040672-MU
Citation ID:
RRID:MMRRC_040672-MU
Other Names:
R3612 (G1), C57BL/6J-MtgxR3612Btlr
Major Collection:

Strain Information

Glrb
Name: glycine receptor, beta subunit
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 14658
HGNC: HGNC:4329
Homologene: 20224
Ccdc88c
Name: coiled-coil domain containing 88C
Synonyms: Daple, 0610010D24Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 68339
VEGA: 12
Homologene: 18903
Rprd2
Name: regulation of nuclear pre-mRNA domain containing 2
Synonyms: 4930535B03Rik, 2810036A19Rik, 6720469I21Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 75137
Homologene: 19997
Cdc42bpb
Name: CDC42 binding protein kinase beta
Synonyms: DMPK-like
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 217866
VEGA: 12
HGNC: HGNC:1738
Homologene: 55945
Diaph3
Name: diaphanous related formin 3
Synonyms: Diap3, 4930417P13Rik, Drf3, mDia2, p134MDia2
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 56419
VEGA: 14
Homologene: 84829
Ankrd12
Name: ankyrin repeat domain 12
Synonyms: GAC-1, 2900001A12Rik, ANCO-2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 106585
Homologene: 9059
Cep290
Name: centrosomal protein 290
Synonyms: b2b1752Clo, Nphp6, Kiaa, b2b1454Clo
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 216274
VEGA: 10
Homologene: 77213
Ncapd3
Name: non-SMC condensin II complex, subunit D3
Synonyms: B130055D15Rik, 2810487N22Rik, 4632407J06Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 78658
VEGA: 9
Homologene: 41021
Fabp3
Name: fatty acid binding protein 3, muscle and heart
Synonyms: Fabp3, Fabph1, Mdgi, Fabph-1, Fabph4, Fabph-4, H-FABP
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 14077
HGNC: HGNC:3557
Homologene: 68379
Cdh19
Name: cadherin 19, type 2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 227485
HGNC: HGNC:1758
Homologene: 23286
Erc2
Name: ELKS/RAB6-interacting/CAST family member 2
Synonyms: CAST, D14Ertd171e, ELKS2alpha
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 238988
Homologene: 69188
Slc12a7
Name: solute carrier family 12, member 7
Synonyms: Kcc4
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 20499
VEGA: 13
Homologene: 21312
Slc9a2
Name: solute carrier family 9 (sodium/hydrogen exchanger), member 2
Synonyms: NHE2, 4932415O19Rik, 2210416H12Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226999
Homologene: 20661
Klf7
Name: Kruppel-like transcription factor 7 (ubiquitous)
Synonyms: 9830124P08Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 93691
HGNC: HGNC:6350
Homologene: 2751
Mslnl
Name: mesothelin-like
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 328783
VEGA: 17
Homologene: 18633
Ppargc1b
Name: peroxisome proliferative activated receptor, gamma, coactivator 1 beta
Synonyms: 4631412G21Rik, PGC-1beta/ERRL1
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 170826
Homologene: 15776
Cd109
Name: CD109 antigen
Synonyms: Gov platelet alloantigens, 9930012E15Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235505
Homologene: 25183
Adam5
Name: a disintegrin and metallopeptidase domain 5
Synonyms: tMDCII
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 11499
HGNC: HGNC:212
Homologene: 49138
Vps37a
Name: vacuolar protein sorting 37A
Synonyms: D8Ertd531e, 2210018P21Rik, 4930592A21Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 52348
VEGA: 8
Homologene: 45120
Or1e22
Name: olfactory receptor family 1 subfamily E member 22
Synonyms: MOR135-4, GA_x6K02T2P1NL-3646409-3645474, Olfr381
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 259024
Gm4782
Name: predicted gene 4782
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 213320
Fah
Name: fumarylacetoacetate hydrolase
Synonyms: swst
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 14085
HGNC: HGNC:3579
Homologene: 110
Aacs
Name: acetoacetyl-CoA synthetase
Synonyms: 2210408B16Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 78894
Homologene: 11322
Vmn2r17
Name: vomeronasal 2, receptor 17
Synonyms: EG384221
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 384221
Homologene: 104825
Acsm2
Name: acyl-CoA synthetase medium-chain family member 2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233799
Homologene: 70404
Zmynd19
Name: zinc finger, MYND domain containing 19
Synonyms: 2700064H14Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 67187
Homologene: 12093
Cherp
Name: calcium homeostasis endoplasmic reticulum protein
Synonyms: DAN16, SCAF6, D8Wsu96e, 5730408I11Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 27967
Homologene: 4656
Tex24
Name: testis expressed gene 24
Synonyms: TESF-1, 1700108N07Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 541463
Gatc
Name: glutamyl-tRNA amidotransferase subunit C
Synonyms: 2010003O18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 384281
Homologene: 19922
Dcstamp
Name: dendrocyte expressed seven transmembrane protein
Synonyms: Tm7sf4, DC-STAMP, 4833414I07Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 75766
VEGA: 15
Homologene: 12769
Ech1
Name: enoyl coenzyme A hydratase 1, peroxisomal
Synonyms: dienoyl-CoA isomerase
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 51798
HGNC: HGNC:3149
Homologene: 1069
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 40,719,058 bp
  • A to G, chromosome 1 at 64,035,773 bp
  • A to T, chromosome 1 at 110,893,296 bp
  • A to G, chromosome 2 at 24,951,480 bp
  • A to G, chromosome 3 at 80,862,030 bp
  • G to A, chromosome 3 at 95,764,152 bp
  • C to T, chromosome 4 at 130,312,387 bp
  • A to G, chromosome 5 at 109,429,597 bp
  • T to A, chromosome 5 at 115,335,486 bp
  • T to C, chromosome 5 at 125,503,187 bp
  • T to A, chromosome 6 at 50,608,630 bp
  • C to T, chromosome 7 at 28,830,243 bp
  • A to G, chromosome 7 at 84,585,290 bp
  • G to C, chromosome 7 at 119,591,330 bp
  • T to C, chromosome 8 at 24,818,089 bp
  • T to A, chromosome 8 at 27,345,173 bp
  • T to C, chromosome 8 at 40,544,936 bp
  • G to A, chromosome 8 at 72,461,996 bp
  • T to A, chromosome 9 at 27,050,357 bp
  • ATTT to ATTTGTTT, chromosome 9 at 78,712,521 bp
  • T to A, chromosome 10 at 100,541,581 bp
  • G to C, chromosome 11 at 60,477,679 bp
  • T to C, chromosome 11 at 73,485,940 bp
  • A to T, chromosome 12 at 100,939,073 bp
  • A to G, chromosome 12 at 111,303,822 bp
  • C to A, chromosome 13 at 73,809,923 bp
  • T to C, chromosome 14 at 27,777,177 bp
  • T to C, chromosome 14 at 87,037,457 bp
  • C to T, chromosome 14 at 96,381,770 bp
  • C to T, chromosome 15 at 39,760,326 bp
  • G to A, chromosome 17 at 25,742,934 bp
  • A to T, chromosome 17 at 65,983,547 bp
  • T to C, chromosome 18 at 61,310,556 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3612 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040672-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.