Strain Name:
C57BL/6J-MtgxR3722Btlr/Mmmh
Stock Number:
040713-MU
Citation ID:
RRID:MMRRC_040713-MU
Other Names:
R3722 (G1), C57BL/6J-MtgxR3722Btlr
Major Collection:

Strain Information

Pou2f1
Name: POU domain, class 2, transcription factor 1
Synonyms: Oct-1C, Otf-1, Oct-1A, Oct-1z, Otf1, Oct-1B, 2810482H01Rik, oct-1, Oct1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 18986
HGNC: HGNC:9212
Homologene: 37658
Pak1
Name: p21 (RAC1) activated kinase 1
Synonyms: Paka, PAK-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 18479
HGNC: HGNC:8590
Homologene: 1936
Atg7
Name: autophagy related 7
Synonyms: Apg7l, 1810013K23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 74244
Homologene: 4662
Mrtfb
Name: myocardin related transcription factor B
Synonyms: Mrtfb, Mkl2, MRTF-B, Gt4-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 239719
Homologene: 40917
Ptprk
Name: protein tyrosine phosphatase receptor type K
Synonyms: PTPk, RPTPkappa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 19272
VEGA: 10
HGNC: HGNC:9674
Homologene: 55693
Slc44a2
Name: solute carrier family 44, member 2
Synonyms: CTL2, 1110028E10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 68682
VEGA: 9
Homologene: 10711
Pelp1
Name: proline, glutamic acid and leucine rich protein 1
Synonyms: 4930563C04Rik, MNAR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 75273
Homologene: 8664
Arhgap21
Name: Rho GTPase activating protein 21
Synonyms: ARHGAP10, 5530401C11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 71435
Homologene: 10822
Alkbh8
Name: alkB homolog 8, tRNA methyltransferase
Synonyms: 4930562C03Rik, Abh8, 8030431D03Rik, 9430088N01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 67667
Homologene: 44488
Braf
Name: Braf transforming gene
Synonyms: 9930012E13Rik, D6Ertd631e, Braf2, Braf-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 109880
HGNC: HGNC:1097
Homologene: 3197
Tex2
Name: testis expressed gene 2
Synonyms: 4930568E07Rik, Def-5, Taz4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 21763
Homologene: 32414
Akap9
Name: A kinase (PRKA) anchor protein (yotiao) 9
Synonyms: AKAP450, repro12, mei2-5, 5730481H23Rik, G1-448-15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100986
HGNC: HGNC:379
Homologene: 134583
Fndc3a
Name: fibronectin type III domain containing 3A
Synonyms: sys, D14Ertd453e, F730017H24Rik, 1700094E19Rik, Fndc3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 319448
Homologene: 8952
Lrmda
Name: leucine rich melanocyte differentiation associated
Synonyms: 1700112E06Rik, Oca7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 76633
Homologene: 49975
Sos1
Name: SOS Ras/Rac guanine nucleotide exchange factor 1
Synonyms: 4430401P03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 20662
VEGA: 17
Homologene: 4117
Vps13b
Name: vacuolar protein sorting 13B
Synonyms: 1810042B05Rik, C330002D13Rik, 2310042E16Rik, Coh1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 666173
VEGA: 15
HGNC: HGNC:2183
Homologene: 49516
Snrnp40
Name: small nuclear ribonucleoprotein 40 (U5)
Synonyms: 0610009C03Rik, Wdr57
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 66585
Homologene: 3538
Rreb1
Name: ras responsive element binding protein 1
Synonyms: 1110037N09Rik, B930013M22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 68750
Homologene: 2218
Mei1
Name: meiotic double-stranded break formation protein 1
Synonyms: mei1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 74369
Homologene: 46535
Ptprs
Name: protein tyrosine phosphatase receptor type S
Synonyms: Ptpt9, PTP-NU3, PTPsigma, RPTPsigma
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 19280
HGNC: HGNC:9681
Homologene: 20626
Rpn1
Name: ribophorin I
Synonyms: D6Wsu137e, Rpn-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 103963
Homologene: 2213
Dnah8
Name: dynein, axonemal, heavy chain 8
Synonyms: Dnahc8, P1-Loop, Hst6.7b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 13417
VEGA: 17
HGNC: HGNC:2952
Homologene: 1049
Ncstn
Name: nicastrin
Synonyms: D1Dau13e, Nct, 9430068N19Rik, nicastrin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 59287
Homologene: 41029
Dolpp1
Name: dolichyl pyrophosphate phosphatase 1
Synonyms: 0610011H20Rik, LSFR2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 57170
Homologene: 10663
Nudt4
Name: nudix hydrolase 4
Synonyms: nudix (nucleoside diphosphate linked moiety X)-type motif 4, HDCMB47P, DIPP2beta, 4933436C10Rik, DIPP2alpha, DIPP2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 71207
VEGA: 10
HGNC: HGNC:8051
Homologene: 41726
Appl1
Name: adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1
Synonyms: 2900057D21Rik, 7330406P05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 72993
Homologene: 32143
H3c8
Name: H3 clustered histone 8
Synonyms: M32460, H3.1-221, Hist1h3g
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 97908
HGNC: HGNC:4767
Homologene: 134479
Spink4
Name: serine peptidase inhibitor, Kazal type 4
Synonyms: MPGC60
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 20731
Homologene: 7954
Atp8b1
Name: ATPase, class I, type 8B, member 1
Synonyms: FIC1, Ic
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 54670
VEGA: 18
HGNC: HGNC:3706
Homologene: 21151
Pde4d
Name: phosphodiesterase 4D, cAMP specific
Synonyms: Dpde3, 9630011N22Rik, dunce
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 238871
HGNC: HGNC:8783
Omp
Name: olfactory marker protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 18378
HGNC: HGNC:8136
Homologene: 36195
Slc35d1
Name: solute carrier family 35 (UDP-glucuronic acid/UDP-N-acetylgalactosamine dual transporter), member D1
Synonyms: UGTREL7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242585
Homologene: 22870
Zbtb5
Name: zinc finger and BTB domain containing 5
Synonyms: 9430083K24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230119
Homologene: 8913
Uggt2
Name: UDP-glucose glycoprotein glucosyltransferase 2
Synonyms: Ugcgl2, A230065J02Rik, 3110027P15Rik, 1810064L21Rik, 3110001A05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 66435
Homologene: 56875
Snapc4
Name: small nuclear RNA activating complex, polypeptide 4
Synonyms: 5730436L13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 227644
Homologene: 2321
Asic3
Name: acid-sensing (proton-gated) ion channel 3
Synonyms: DRASIC, Accn3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 171209
HGNC: HGNC:101
Homologene: 20999
Slc7a1
Name: solute carrier family 7 (cationic amino acid transporter, y+ system), member 1
Synonyms: 4831426K01Rik, Atrc1, Rev-1, Cat1, Rec-1, Atrc-1, mCAT-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 11987
Homologene: 20658
Rnf135
Name: ring finger protein 135
Synonyms: 2410006N06Rik, MGC13061, 0610037N03Rik, U 2-3-0
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 71956
Homologene: 12427
Cacna1s
Name: calcium channel, voltage-dependent, L type, alpha 1S subunit
Synonyms: Cav1.1, Cchl1a3, fmd, mdg, sj, DHPR alpha1s, muscle dysgenesis
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12292
HGNC: HGNC:1397
Homologene: 37257
Ttc23l
Name: tetratricopeptide repeat domain 23-like
Synonyms: 4930401A09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 75777
Homologene: 44907
Adgb
Name: androglobin
Synonyms: 9130014G24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 215772
Homologene: 100289
Dnai1
Name: dynein axonemal intermediate chain 1
Synonyms: b2b1526Clo, Dnaic1, 1110066F04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 68922
HGNC: HGNC:2954
Homologene: 8122
Sipa1l2
Name: signal-induced proliferation-associated 1 like 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244668
Homologene: 18956
Kbtbd11
Name: kelch repeat and BTB (POZ) domain containing 11
Synonyms: 4930465M17Rik, 2900016B01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 74901
Homologene: 28701
Ism1
Name: isthmin 1, angiogenesis inhibitor
Synonyms: 5430433G21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 319909
Homologene: 19061
Rars2
Name: arginyl-tRNA synthetase 2, mitochondrial
Synonyms: 1500002I10Rik, PRO1992, Rarsl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 109093
Homologene: 5707
Spata31f1a
Name: spermatogenesis associated 31 subfamily F member 1A
Synonyms: Fam205a1, Gm12429
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 433698
Homologene: 79022
Ttll6
Name: tubulin tyrosine ligase-like family, member 6
Synonyms: D11Moh44e, 4932418K24Rik, t8130b59, D11Moh43e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237930
Homologene: 18229
Txk
Name: TXK tyrosine kinase
Synonyms: Btkl, A130089B16Rik, PTK4, Rlk
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 22165
Homologene: 2497
Fbxl12
Name: F-box and leucine-rich repeat protein 12
Synonyms: 3110048D16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 30843
Homologene: 32198
Kcnk15
Name: potassium channel, subfamily K, member 15
Synonyms: KT3.3, KCNK14, KCNK11, TASK5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 241769
Homologene: 11179
Or10d3
Name: olfactory receptor family 10 subfamily D member 3
Synonyms: GA_x6K02T2PVTD-33247839-33246901, Olfr958, MOR224-9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258327
VEGA: 9
HGNC: HGNC:8168
Homologene: 27128
Fam170a
Name: family with sequence similarity 170, member A
Synonyms: Znfd, LOC225497
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 225497
VEGA: 18
Homologene: 86702
Zfp760
Name: zinc finger protein 760
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 240034
VEGA: 17
Or4a75
Name: olfactory receptor family 4 subfamily A member 75
Synonyms: MOR231-10, Olfr1248, GA_x6K02T2Q125-51059648-51058725
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258787
Homologene: 133613
Btnl2
Name: butyrophilin-like 2
Synonyms: BTLN2, BTL-II, butyrophylin-like MHC class II associated, NG9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 547431
HGNC: HGNC:1142
Homologene: 10482
Tmcc1
Name: transmembrane and coiled coil domains 1
Synonyms: 3632431M01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 330401
Homologene: 8995
Cd47
Name: CD47 antigen (Rh-related antigen, integrin-associated signal transducer)
Synonyms: B430305P08Rik, IAP, 9130415E20Rik, Itgp, integrin-associated protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 16423
HGNC: HGNC:1682
Homologene: 1346
Dlg2
Name: discs large MAGUK scaffold protein 2
Synonyms: A330103J02Rik, Chapsyn-110, PSD93, LOC382816, B330007M19Rik, Dlgh2, B230218P12Rik, Gm21505
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 23859
HGNC: HGNC:2901
Homologene: 1046
Slc35a5
Name: solute carrier family 35, member A5
Synonyms: 1010001J06Rik, D730043G07Rik, D16Ertd450e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 74102
Homologene: 9930
Gm12886
Name: predicted gene 12886
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 666921
Homologene: 111034
Uros
Name: uroporphyrinogen III synthase
Synonyms: URO-synthase, uroporphyrinogen-III synthase, hydroxymethylbilane hydrolyase (cyclizing), Uros3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 22276
Homologene: 37296
Diras2
Name: DIRAS family, GTP-binding RAS-like 2
Synonyms: 2900052J15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 68203
VEGA: 13
Homologene: 56777
Cox8b
Name: cytochrome c oxidase subunit 8B
Synonyms: COX VIII-H, COX8H
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 12869
Homologene: 7277
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 136,069,042 bp
  • G to C, chromosome 1 at 165,894,969 bp
  • G to A, chromosome 1 at 172,067,895 bp
  • T to C, chromosome 2 at 20,850,291 bp
  • A to G, chromosome 2 at 26,365,428 bp
  • T to C, chromosome 2 at 30,397,488 bp
  • A to T, chromosome 2 at 89,618,159 bp
  • A to T, chromosome 2 at 139,732,011 bp
  • T to C, chromosome 2 at 163,858,294 bp
  • T to A, chromosome 4 at 34,659,970 bp
  • T to A, chromosome 4 at 40,929,136 bp
  • G to A, chromosome 4 at 41,602,615 bp
  • T to C, chromosome 4 at 42,851,472 bp
  • A to G, chromosome 4 at 44,994,863 bp
  • T to C, chromosome 4 at 103,208,124 bp
  • T to A, chromosome 4 at 121,417,470 bp
  • C to T, chromosome 4 at 130,368,275 bp
  • A to G, chromosome 5 at 4,070,351 bp
  • A to T, chromosome 5 at 24,416,999 bp
  • T to A, chromosome 5 at 72,707,735 bp
  • G to A, chromosome 5 at 148,335,533 bp
  • G to A, chromosome 6 at 39,623,676 bp
  • G to A, chromosome 6 at 88,090,300 bp
  • A to G, chromosome 6 at 114,695,663 bp
  • T to C, chromosome 6 at 116,133,822 bp
  • T to A, chromosome 7 at 91,711,800 bp
  • C to T, chromosome 7 at 97,854,497 bp
  • T to C, chromosome 7 at 98,145,213 bp
  • A to T, chromosome 7 at 133,702,391 bp
  • T to A, chromosome 7 at 140,899,005 bp
  • T to A, chromosome 8 at 15,029,118 bp
  • G to A, chromosome 8 at 125,473,584 bp
  • T to C, chromosome 9 at 3,385,153 bp
  • A to T, chromosome 9 at 20,638,972 bp
  • A to T, chromosome 9 at 21,342,977 bp
  • A to C, chromosome 9 at 39,550,122 bp
  • A to T, chromosome 10 at 10,340,510 bp
  • A to G, chromosome 10 at 28,383,623 bp
  • A to T, chromosome 10 at 95,549,505 bp
  • T to C, chromosome 11 at 70,398,200 bp
  • G to T, chromosome 11 at 80,196,917 bp
  • A to G, chromosome 11 at 96,133,921 bp
  • C to T, chromosome 11 at 106,546,740 bp
  • T to C, chromosome 13 at 23,535,552 bp
  • T to A, chromosome 13 at 37,947,098 bp
  • T to A, chromosome 13 at 52,508,023 bp
  • T to A, chromosome 13 at 109,951,332 bp
  • T to A, chromosome 14 at 22,027,331 bp
  • G to A, chromosome 14 at 26,927,844 bp
  • T to A, chromosome 14 at 72,540,208 bp
  • T to C, chromosome 14 at 119,041,518 bp
  • CT to CTTGGATT, chromosome 15 at 10,537,562 bp
  • G to A, chromosome 15 at 10,537,566 bp
  • T to A, chromosome 15 at 35,671,382 bp
  • A to G, chromosome 15 at 82,103,204 bp
  • C to T, chromosome 16 at 13,385,693 bp
  • A to T, chromosome 16 at 45,147,322 bp
  • A to G, chromosome 16 at 49,867,842 bp
  • A to G, chromosome 17 at 21,722,162 bp
  • G to A, chromosome 17 at 30,854,898 bp
  • T to C, chromosome 17 at 34,358,135 bp
  • A to G, chromosome 17 at 56,417,485 bp
  • A to T, chromosome 17 at 80,413,727 bp
  • C to T, chromosome 18 at 50,282,204 bp
  • A to C, chromosome 18 at 64,541,266 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3722 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040713-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.