Strain Name:
C57BL/6J-MtgxR3732Btlr/Mmmh
Stock Number:
040720-MU
Citation ID:
RRID:MMRRC_040720-MU
Other Names:
R3732 (G1), C57BL/6J-MtgxR3732Btlr
Major Collection:

Gene Information

Gene Symbol: Espl1 [MGI:2146156] (Mus musculus (mouse))
Name: extra spindle pole bodies 1, separase
Synonyms: PRCE, PRCE, Cerp, separase, ESP1, SSE
Chromosome: 15
Alteration at locus: Chemically Induced
Gene Symbol: Chrna3 [MGI:87887] (Mus musculus (mouse))
Name: cholinergic receptor, nicotinic, alpha polypeptide 3
Synonyms: Acra-3, (a)3, A730007P14Rik, alpha 3, neuronal nicotinic acetylcholine receptor, alpha 3 subunit, Acra3
Chromosome: 9
Alteration at locus: Chemically Induced
Gene Symbol: Lgi1 [MGI:1861691] (Mus musculus (mouse))
Name: leucine-rich repeat LGI family, member 1
Chromosome: 19
Alteration at locus: Chemically Induced
Gene Symbol: Lars [MGI:1913808] (Mus musculus (mouse))
Name: leucyl-tRNA synthetase
Synonyms: 2310045K21Rik, 3110009L02Rik
Chromosome: 18
Alteration at locus: Chemically Induced
Gene Symbol: Zfp934 [MGI:1924367] (Mus musculus (mouse))
Name: zinc finger protein 934
Synonyms: 6720457D02Rik
Chromosome: 13
Alteration at locus: Chemically Induced
Gene Symbol: Oxr1 [MGI:2179326] (Mus musculus (mouse))
Name: oxidation resistance 1
Synonyms: 2210416C20Rik, C7, C7B
Chromosome: 15
Alteration at locus: Chemically Induced
Gene Symbol: Adgrl3 [MGI:2441950] (Mus musculus (mouse))
Name: adhesion G protein-coupled receptor L3
Synonyms: D130075K09Rik, lectomedin 3, LEC3, Lphn3, 5430402I23Rik
Chromosome: 5
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 36,693,260 bp
  • A to T, chromosome 1 at 164,054,740 bp
  • C to A, chromosome 1 at 186,968,097 bp
  • C to A, chromosome 1 at 188,944,760 bp
  • A to T, chromosome 2 at 10,105,670 bp
  • T to C, chromosome 2 at 25,938,344 bp
  • A to G, chromosome 2 at 32,367,483 bp
  • G to A, chromosome 2 at 32,566,292 bp
  • G to C, chromosome 2 at 73,156,860 bp
  • C to A, chromosome 2 at 74,847,262 bp
  • G to A, chromosome 2 at 91,810,131 bp
  • T to A, chromosome 2 at 122,345,207 bp
  • A to G, chromosome 2 at 163,853,813 bp
  • T to A, chromosome 2 at 164,088,908 bp
  • A to T, chromosome 3 at 36,638,559 bp
  • T to C, chromosome 3 at 49,754,791 bp
  • A to G, chromosome 3 at 58,676,250 bp
  • A to G, chromosome 3 at 59,325,714 bp
  • T to A, chromosome 4 at 45,898,542 bp
  • C to T, chromosome 4 at 59,207,798 bp
  • C to T, chromosome 4 at 121,148,324 bp
  • A to G, chromosome 4 at 126,923,054 bp
  • T to C, chromosome 4 at 135,463,846 bp
  • G to A, chromosome 4 at 138,833,344 bp
  • G to T, chromosome 4 at 156,334,397 bp
  • C to A, chromosome 5 at 81,794,946 bp
  • G to A, chromosome 5 at 104,489,419 bp
  • C to T, chromosome 5 at 125,506,262 bp
  • A to G, chromosome 5 at 135,180,913 bp
  • T to C, chromosome 6 at 48,449,930 bp
  • A to G, chromosome 6 at 80,019,655 bp
  • T to C, chromosome 6 at 83,313,475 bp
  • C to T, chromosome 6 at 94,611,576 bp
  • T to C, chromosome 6 at 108,841,621 bp
  • A to T, chromosome 6 at 124,910,140 bp
  • T to C, chromosome 6 at 146,382,700 bp
  • T to C, chromosome 6 at 148,202,522 bp
  • C to T, chromosome 7 at 25,803,954 bp
  • A to G, chromosome 7 at 26,312,827 bp
  • A to G, chromosome 7 at 46,288,368 bp
  • T to G, chromosome 7 at 86,412,633 bp
  • A to T, chromosome 7 at 107,182,367 bp
  • C to T, chromosome 8 at 3,613,443 bp
  • T to C, chromosome 8 at 44,953,269 bp
  • T to A, chromosome 8 at 63,345,699 bp
  • C to G, chromosome 8 at 121,753,349 bp
  • G to A, chromosome 8 at 123,501,233 bp
  • C to T, chromosome 9 at 4,513,295 bp
  • GTTCTTCT to GTTCT, chromosome 9 at 15,006,171 bp
  • C to T, chromosome 9 at 42,392,106 bp
  • T to A, chromosome 9 at 51,059,544 bp
  • A to G, chromosome 9 at 54,338,662 bp
  • T to C, chromosome 9 at 55,015,894 bp
  • C to T, chromosome 9 at 66,445,640 bp
  • T to A, chromosome 10 at 63,820,207 bp
  • C to T, chromosome 11 at 101,988,452 bp
  • G to T, chromosome 11 at 109,794,154 bp
  • T to A, chromosome 11 at 116,273,956 bp
  • T to C, chromosome 12 at 83,269,507 bp
  • T to C, chromosome 12 at 101,987,308 bp
  • T to A, chromosome 13 at 3,854,337 bp
  • T to C, chromosome 13 at 27,579,733 bp
  • T to C, chromosome 13 at 34,075,264 bp
  • T to A, chromosome 13 at 56,527,017 bp
  • A to T, chromosome 13 at 62,517,785 bp
  • G to A, chromosome 13 at 64,740,999 bp
  • T to C, chromosome 13 at 81,556,956 bp
  • T to C, chromosome 13 at 91,524,607 bp
  • T to A, chromosome 13 at 112,611,982 bp
  • G to A, chromosome 14 at 34,736,764 bp
  • A to T, chromosome 15 at 28,409,122 bp
  • T to C, chromosome 15 at 41,848,701 bp
  • A to G, chromosome 15 at 102,312,989 bp
  • G to T, chromosome 16 at 33,053,626 bp
  • T to A, chromosome 16 at 42,144,572 bp
  • A to C, chromosome 16 at 73,920,747 bp
  • A to G, chromosome 17 at 30,192,656 bp
  • A to G, chromosome 17 at 31,448,427 bp
  • C to T, chromosome 17 at 42,672,581 bp
  • A to G, chromosome 18 at 37,664,123 bp
  • G to A, chromosome 18 at 38,332,052 bp
  • T to C, chromosome 18 at 42,212,602 bp
  • A to C, chromosome 19 at 7,374,301 bp
  • T to C, chromosome 19 at 12,587,550 bp
  • T to G, chromosome 19 at 21,938,252 bp
  • T to C, chromosome 19 at 38,306,246 bp
  • A to T, chromosome 19 at 57,049,460 bp
  • TACACACACACACACACA to TACACACACACACACA, chromosome X at 20,850,226 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3732 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040720-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.