Strain Name:
C57BL/6J-MtgxR3734Btlr/Mmmh
Stock Number:
040721-MU
Citation ID:
RRID:MMRRC_040721-MU
Other Names:
R3734 (G1), C57BL/6J-MtgxR3734Btlr
Major Collection:

Strain Information

Stk4
Name: serine/threonine kinase 4
Synonyms: Ysk3, Kas-2, Mst1, sterile 20-like kinase 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 58231
Homologene: 55965
Nap1l4
Name: nucleosome assembly protein 1-like 4
Synonyms: Nap2, D7Wsu30e, 2810410H14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 17955
HGNC: HGNC:7640
Homologene: 133933
Golga1
Name: golgi autoantigen, golgin subfamily a, 1
Synonyms: golgin-97, 0710001G09Rik, 2210418B03Rik, Golgi97, awag
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 76899
HGNC: HGNC:4424
Homologene: 68223
Kif2c
Name: kinesin family member 2C
Synonyms: 4930402F02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 73804
HGNC: HGNC:6393
Homologene: 21355
Panx1
Name: pannexin 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 55991
HGNC: HGNC:8599
Homologene: 49416
Ift122
Name: intraflagellar transport 122
Synonyms: C86139, Wdr10, sopb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 81896
Homologene: 12819
Robo2
Name: roundabout guidance receptor 2
Synonyms: 9430089E08Rik, D230004I22Rik, 2600013A04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 268902
Homologene: 43188
Dsg2
Name: desmoglein 2
Synonyms: D18Ertd293e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 13511
HGNC: HGNC:3049
Homologene: 1464
Scmh1
Name: sex comb on midleg homolog 1
Synonyms: Scml3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 29871
Homologene: 32146
Nipal3
Name: NIPA-like domain containing 3
Synonyms: 9130020G22Rik, Npal3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 74552
Homologene: 10722
2700049A03Rik
Name: RIKEN cDNA 2700049A03 gene
Synonyms: talpid3, Ta3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 76967
Homologene: 8839
Eps8l2
Name: EPS8-like 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 98845
Homologene: 69358
Skor1
Name: SKI family transcriptional corepressor 1
Synonyms: C230094B15Rik, Corl1, Lbxcor1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 207667
Homologene: 18175
Ulk4
Name: unc-51-like kinase 4
Synonyms: 4932415A06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 209012
Homologene: 41205
Iqcg
Name: IQ motif containing G
Synonyms: G1-374-12, stubby12d, repro1, 2400003L07Rik, esgd12d
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 69707
Homologene: 32776
Muc5b
Name: mucin 5, subtype B, tracheobronchial
Synonyms: MUC9, MUC5, 2300002I04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 74180
HGNC: HGNC:7516
Homologene: 136756
Edem1
Name: ER degradation enhancer, mannosidase alpha-like 1
Synonyms: A130059K23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 192193
Homologene: 33836
Unc5d
Name: unc-5 netrin receptor D
Synonyms: Unc5h4, D930029E11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 210801
Homologene: 15450
Ablim1
Name: actin-binding LIM protein 1
Synonyms: Limab1, 4833406P10Rik, abLIM-S, abLIM-M, abLIM-L, 2610209L21Rik, 9330196J19Rik, 2210411C18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 226251
HGNC: HGNC:78
Homologene: 40994
Nol4
Name: nucleolar protein 4
Synonyms: 4930568N03Rik, LOC383304, 1700013J13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 319211
HGNC: HGNC:7870
Homologene: 36142
Itga1
Name: integrin alpha 1
Synonyms: CD49A, Vla1, E130012M19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 109700
VEGA: 13
HGNC: HGNC:6134
Homologene: 57137
Map3k6
Name: mitogen-activated protein kinase kinase kinase 6
Synonyms: Ask2, MEKK6, MAPKKK6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 53608
HGNC: HGNC:6858
Homologene: 3435
Pbld1
Name: phenazine biosynthesis-like protein domain containing 1
Synonyms: 0610038K03Rik, Pbld
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 68371
VEGA: 10
Homologene: 41470
Tpte
Name: transmembrane phosphatase with tensin homology
Synonyms: Pten2, Vsp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234129
Homologene: 50411
Vmn2r16
Name: vomeronasal 2, receptor 16
Synonyms: EG384220
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 384220
Homologene: 104825
Ergic2
Name: ERGIC and golgi 2
Synonyms: 4930572C01Rik, 1200009B18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 67456
Homologene: 6574
Cyp2a4
Name: cytochrome P450, family 2, subfamily a, polypeptide 4
Synonyms: Cyp15a1, D7Ucla4, testosterone 15alpha-hydroxylase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 13086
Homologene: 85917
Tenm1
Name: teneurin transmembrane protein 1
Synonyms: Ten-m1, TCAP-1, teneurin-1, Odz1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 23963
HGNC: HGNC:8117
Homologene: 56542
Or2l13b
Name: olfactory receptor family 2 subfamily L member 13B
Synonyms: GA_x54KRFPKG5P-15979009-15978071, MOR271-1, Olfr168
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 258354
Homologene: 128057
Slc1a7
Name: solute carrier family 1 (glutamate transporter), member 7
Synonyms: EAAT5, A930031E15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242607
Homologene: 21327
Lsamp
Name: limbic system-associated membrane protein
Synonyms: Lamp, Lam, B130007O04Rik, D930023J12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 268890
HGNC: HGNC:6705
Homologene: 20533
Jrkl
Name: Jrk-like
Synonyms: C030035D04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 77532
VEGA: 9
HGNC: HGNC:6200
Homologene: 2797
Lag3
Name: lymphocyte-activation gene 3
Synonyms: CD223, Ly66, LAG-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 16768
HGNC: HGNC:6476
Homologene: 1719
Ccdc87
Name: coiled-coil domain containing 87
Synonyms: 4931419P11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 399599
VEGA: 19
Homologene: 49531
Fcgr3
Name: Fc receptor, IgG, low affinity III
Synonyms: Fcg receptor III, CD16, FcgammaRIII
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 14131
HGNC: HGNC:3616
Homologene: 47936
Gldn
Name: gliomedin
Synonyms: Crlg2, CRG-L2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235379
Homologene: 14529
Gm9933
Name: predicted gene 9933
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 171,057,874 bp
  • G to T, chromosome 2 at 39,050,170 bp
  • T to A, chromosome 2 at 164,088,908 bp
  • G to C, chromosome 4 at 107,977,644 bp
  • T to C, chromosome 4 at 117,162,646 bp
  • T to A, chromosome 4 at 120,478,080 bp
  • T to C, chromosome 4 at 133,248,396 bp
  • T to C, chromosome 4 at 135,463,846 bp
  • T to C, chromosome 5 at 109,330,414 bp
  • T to C, chromosome 6 at 108,841,621 bp
  • A to G, chromosome 6 at 115,925,501 bp
  • A to T, chromosome 6 at 124,910,140 bp
  • T to C, chromosome 6 at 148,202,522 bp
  • A to G, chromosome 7 at 26,312,827 bp
  • A to C, chromosome 7 at 141,357,821 bp
  • C to T, chromosome 7 at 141,859,037 bp
  • A to C, chromosome 7 at 143,534,395 bp
  • G to A, chromosome 8 at 22,359,482 bp
  • T to C, chromosome 8 at 28,761,798 bp
  • C to A, chromosome 8 at 71,676,581 bp
  • A to G, chromosome 9 at 13,245,530 bp
  • GTTCTTCT to GTTCT, chromosome 9 at 15,006,171 bp
  • A to G, chromosome 9 at 54,338,662 bp
  • A to T, chromosome 9 at 63,140,068 bp
  • T to C, chromosome 9 at 121,261,989 bp
  • T to C, chromosome 10 at 63,071,465 bp
  • G to T, chromosome 12 at 71,164,546 bp
  • A to T, chromosome 12 at 71,164,547 bp
  • T to C, chromosome 13 at 114,977,639 bp
  • T to A, chromosome 16 at 19,530,648 bp
  • G to T, chromosome 16 at 33,053,626 bp
  • T to C, chromosome 16 at 42,144,770 bp
  • A to C, chromosome 16 at 73,920,747 bp
  • T to C, chromosome 18 at 20,601,947 bp
  • A to T, chromosome 18 at 22,770,864 bp
  • G to T, chromosome 19 at 4,841,923 bp
  • A to T, chromosome 19 at 57,049,460 bp
  • C to T, chromosome X at 42,684,067 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3734 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040721-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.