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Strain Name:
C57BL/6J-MtgxR3861Btlr/Mmmh
Stock Number:
040788-MU
Citation ID:
RRID:MMRRC_040788-MU
Other Names:
R3861 (G1), C57BL/6J-MtgxR3861Btlr
Major Collection:

Strain Information

Kif3a
Name: kinesin family member 3A
Synonyms: kinesin-II subunit, Kns3, Kif3, Kifl, N-4 kinesin
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16568
HGNC: HGNC:6319
Homologene: 38266
Elp2
Name: elongator acetyltransferase complex subunit 2
Synonyms: Stat3-interacting protein, StIP1, Statip1
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 58523
VEGA: 18
Homologene: 6019
Ptprz1
Name: protein tyrosine phosphatase receptor type Z, polypeptide 1
Synonyms: DSD-1-PG, phosphacan, PTPzeta, PTPbeta, Rptpbeta, Ptpz, Ptprz, RPTPz
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 19283
HGNC: HGNC:9685
Homologene: 2136
Chgb
Name: chromogranin B
Synonyms: secretogranin I, Scg-1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 12653
HGNC: HGNC:1930
Homologene: 1375
Ddx50
Name: DExD box helicase 50
Synonyms: GU2, RH-II/Gubeta, 4933429B04Rik, 8430408E17Rik, DEAD (Asp-Glu-Ala-Asp) box polypeptide 50
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 94213
VEGA: 10
Homologene: 56986
Nsfl1c
Name: NSFL1 (p97) cofactor (p47)
Synonyms: p47
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 386649
Homologene: 41114
Smg7
Name: SMG7 nonsense mediated mRNA decay factor
Synonyms: 9430023P16Rik, Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans)
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226517
Homologene: 32235
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 11,588,554 bp
  • C to T, chromosome 1 at 20,200,927 bp
  • G to A, chromosome 1 at 24,326,017 bp
  • T to C, chromosome 1 at 45,380,237 bp
  • A to G, chromosome 1 at 82,690,672 bp
  • C to T, chromosome 1 at 152,852,598 bp
  • T to A, chromosome 1 at 162,311,405 bp
  • A to T, chromosome 1 at 165,429,269 bp
  • G to T, chromosome 2 at 82,984,776 bp
  • C to A, chromosome 2 at 121,009,133 bp
  • G to C, chromosome 2 at 121,160,731 bp
  • T to A, chromosome 2 at 132,793,144 bp
  • C to A, chromosome 2 at 151,510,904 bp
  • T to A, chromosome 2 at 179,874,097 bp
  • C to T, chromosome 3 at 19,135,032 bp
  • T to C, chromosome 3 at 36,450,658 bp
  • A to G, chromosome 4 at 9,624,353 bp
  • A to G, chromosome 4 at 14,902,506 bp
  • T to C, chromosome 4 at 100,407,923 bp
  • A to G, chromosome 4 at 140,515,487 bp
  • A to T, chromosome 5 at 21,683,289 bp
  • T to C, chromosome 5 at 70,973,543 bp
  • C to T, chromosome 5 at 148,313,413 bp
  • T to C, chromosome 6 at 18,423,833 bp
  • T to C, chromosome 6 at 23,036,895 bp
  • A to G, chromosome 6 at 23,355,861 bp
  • A to G, chromosome 6 at 101,172,371 bp
  • A to C, chromosome 7 at 31,135,940 bp
  • T to C, chromosome 7 at 80,320,368 bp
  • A to G, chromosome 7 at 86,386,123 bp
  • T to A, chromosome 7 at 88,129,994 bp
  • T to C, chromosome 7 at 100,480,251 bp
  • A to T, chromosome 7 at 126,501,951 bp
  • T to C, chromosome 7 at 128,116,513 bp
  • A to G, chromosome 7 at 132,054,919 bp
  • T to A, chromosome 8 at 22,678,836 bp
  • A to T, chromosome 8 at 41,407,783 bp
  • G to A, chromosome 8 at 64,982,827 bp
  • A to G, chromosome 9 at 44,631,461 bp
  • G to T, chromosome 9 at 104,039,326 bp
  • A to T, chromosome 10 at 14,685,019 bp
  • T to A, chromosome 10 at 18,224,995 bp
  • C to A, chromosome 10 at 62,642,946 bp
  • A to G, chromosome 10 at 69,706,932 bp
  • G to A, chromosome 10 at 77,041,975 bp
  • T to A, chromosome 10 at 90,069,558 bp
  • T to C, chromosome 11 at 53,597,978 bp
  • T to C, chromosome 11 at 66,052,994 bp
  • T to C, chromosome 11 at 106,326,124 bp
  • G to A, chromosome 12 at 18,533,410 bp
  • C to T, chromosome 12 at 54,701,612 bp
  • G to T, chromosome 12 at 59,109,021 bp
  • A to G, chromosome 12 at 75,966,479 bp
  • T to C, chromosome 13 at 50,246,851 bp
  • T to A, chromosome 13 at 78,195,675 bp
  • A to T, chromosome 14 at 26,894,679 bp
  • T to A, chromosome 14 at 52,237,121 bp
  • A to T, chromosome 17 at 24,997,614 bp
  • A to G, chromosome 17 at 32,528,104 bp
  • A to T, chromosome 17 at 53,516,016 bp
  • T to C, chromosome 17 at 74,445,621 bp
  • A to T, chromosome 17 at 75,359,338 bp
  • C to T, chromosome 18 at 24,606,920 bp
  • T to C, chromosome 18 at 42,153,319 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3861 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040788-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.