Strain Name:
C57BL/6J-MtgxR3954Btlr/Mmmh
Stock Number:
040831-MU
Citation ID:
RRID:MMRRC_040831-MU
Other Names:
R3954 (G1), C57BL/6J-MtgxR3954Btlr
Major Collection:

Gene Information

Pi4k2a
Name: phosphatidylinositol 4-kinase type 2 alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 84095
VEGA: 19
Homologene: 101681
Vldlr
Name: very low density lipoprotein receptor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 22359
Homologene: 443
Omd
Name: osteomodulin
Synonyms: OSAD, SLRR2C, osteoadherin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 27047
VEGA: 13
HGNC: HGNC:8134
Homologene: 3677
Ncam2
Name: neural cell adhesion molecule 2
Synonyms: Ocam, RNCAM, Ncam-2, R4B12 antigen
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 17968
HGNC: HGNC:7657
Homologene: 3336
Dlg1
Name: discs large MAGUK scaffold protein 1
Synonyms: B130052P05Rik, Dlgh1, SAP97
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 13383
HGNC: HGNC:2900
Homologene: 20869
Actn4
Name: actinin alpha 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 60595
HGNC: HGNC:166
Homologene: 55857
Nup210l
Name: nucleoporin 210-like
Synonyms: 4930548O11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 77595
Homologene: 28122
Carmil1
Name: capping protein regulator and myosin 1 linker 1
Synonyms: Carmil, Lrrc16a, Lrrc16, 1110037D04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 68732
Homologene: 9757
Zc3h13
Name: zinc finger CCCH type containing 13
Synonyms: 4930570G11Rik, 2600010B19Rik, 3110050K21Rik, C87618
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 67302
VEGA: 14
Zfr
Name: zinc finger RNA binding protein
Synonyms: C920030H05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 22763
Homologene: 8009
Ankle2
Name: ankyrin repeat and LEM domain containing 2
Synonyms: D5Ertd585e, 1110001J12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 71782
Homologene: 35424
Ntng2
Name: netrin G2
Synonyms: Lmnt2, 2610016D08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 171171
Homologene: 13053
Lamp5
Name: lysosomal-associated membrane protein family, member 5
Synonyms: 6330527O06Rik, BAD-LAMP, 3110035N03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 76161
Homologene: 8173
Cpsf3
Name: cleavage and polyadenylation specificity factor 3
Synonyms: 73 kDa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 54451
VEGA: 12
HGNC: HGNC:2326
Homologene: 6499
Aagab
Name: alpha- and gamma-adaptin binding protein
Synonyms: 2310007F21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 66939
VEGA: 9
Homologene: 11648
Tmem131l
Name: transmembrane 131 like
Synonyms: D930015E06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229473
Homologene: 9057
Me2
Name: malic enzyme 2, NAD(+)-dependent, mitochondrial
Synonyms: D030040L20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 107029
VEGA: 18
HGNC: HGNC:6984
Homologene: 37615
Ackr4
Name: atypical chemokine receptor 4
Synonyms: CCBP2, PPR1, Ccrl1, VSHK1, CCR11, A630091E18Rik, CCX-CKR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 252837
HGNC: HGNC:1611
Homologene: 49474
Arsi
Name: arylsulfatase i
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 545260
Homologene: 44428
Ndst4
Name: N-deacetylase/N-sulfotransferase (heparin glucosaminyl) 4
Synonyms: 4930439H17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 64580
Homologene: 11208
Hivep2
Name: human immunodeficiency virus type I enhancer binding protein 2
Synonyms: Gm20114, Schnurri-2, Shn-2, MIBP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 15273
HGNC: HGNC:4921
Homologene: 4900
Clspn
Name: claspin
Synonyms: C85083, E130314M08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 269582
Homologene: 11138
Prph2
Name: peripherin 2
Synonyms: Rd2, Nmf193, rds, Tspan22
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 19133
HGNC: HGNC:9942
Homologene: 273
Dennd5a
Name: DENN/MADD domain containing 5A
Synonyms: 1500012B19Rik, ORF37, Rab6ip1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 19347
Homologene: 14584
2210016F16Rik
Name: RIKEN cDNA 2210016F16 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 70153
Homologene: 13005
Cgnl1
Name: cingulin-like 1
Synonyms: 9930020M10Rik, 4933421H10Rik, Jacop
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 68178
Homologene: 41901
Vmn1r124
Name: vomeronasal 1 receptor 124
Synonyms: Gm9500
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 670764
Homologene: 104166
Il18
Name: interleukin 18
Synonyms: Igif, Il-18
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 16173
VEGA: 9
HGNC: HGNC:5986
Homologene: 1200
Adam2
Name: a disintegrin and metallopeptidase domain 2
Synonyms: Ph30-beta, fertilin beta, Ftnb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 11495
VEGA: 14
HGNC: HGNC:198
Homologene: 1127
Gm13128
Name: predicted gene 13128
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 626995
Homologene: 129642
Cfap52
Name: cilia and flagella associated protein 52
Synonyms: 1700019F09Rik, Wdr16, 4933417B11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 71860
Homologene: 12416
B3galnt2
Name: UDP-GalNAc:betaGlcNAc beta 1,3-galactosaminyltransferase, polypeptide 2
Synonyms: A930105D20Rik, D230016N13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 97884
VEGA: 13
Homologene: 17595
Nkx6-3
Name: NK6 homeobox 3
Synonyms: 9130417I07Rik, Nkx6.3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 74561
Homologene: 46375
Cyp3a57
Name: cytochrome P450, family 3, subfamily a, polypeptide 57
Synonyms: EG622127
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 622127
Homologene: 135775
Tec
Name: tec protein tyrosine kinase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 21682
Homologene: 1302
9330159F19Rik
Name: RIKEN cDNA 9330159F19 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 212448
VEGA: 10
Homologene: 8804
Arhgef19
Name: Rho guanine nucleotide exchange factor (GEF) 19
Synonyms: WGEF, 6430573B13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 213649
Homologene: 17710
Veph1
Name: ventricular zone expressed PH domain-containing 1
Synonyms: Veph, 2810471M23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 72789
Homologene: 11624
Galnt6
Name: polypeptide N-acetylgalactosaminyltransferase 6
Synonyms: GalNAc-T6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 207839
HGNC: HGNC:4128
Homologene: 5218
Slc47a1
Name: solute carrier family 47, member 1
Synonyms: mMATE1, 1300013J15Rik, MATE1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 67473
Homologene: 34364
Trim30d
Name: tripartite motif-containing 30D
Synonyms: TRIM30-3, Trim79
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 209387
Homologene: 114426
Rasgrf2
Name: RAS protein-specific guanine nucleotide-releasing factor 2
Synonyms: 6330417G04Rik, Grf2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 19418
HGNC: HGNC:9876
Homologene: 2169
4921506M07Rik
Name: RIKEN cDNA 4921506M07 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Slc26a1
Name: solute carrier family 26 (sulfate transporter), member 1
Synonyms: Sat1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231583
Homologene: 32539
Dusp13
Name: dual specificity phosphatase 13
Synonyms: TMDP, TS-DSP6, LOC382853, LMW-DSP6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 27389
Homologene: 121602
Ccdc114
Name: coiled-coil domain containing 114
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 211535
Homologene: 128610
Olfr566
Name: olfactory receptor 566
Synonyms: MOR14-7P, GA_x6K02T2PBJ9-5560696-5559746
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258168
Homologene: 133721
Hbq1a
Name: hemoglobin, theta 1A
Synonyms: Hbq1, LOC380691, A230090E05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216635
HGNC: HGNC:4833
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 2 at 29,207,535 bp
  • G to A, chromosome 2 at 136,061,008 bp
  • A to G, chromosome 3 at 66,172,196 bp
  • C to A, chromosome 3 at 83,910,419 bp
  • A to T, chromosome 3 at 90,193,054 bp
  • T to C, chromosome 3 at 125,437,905 bp
  • ACGGCGGCGGC to A, chromosome 4 at 126,566,437 bp
  • A to G, chromosome 4 at 141,256,334 bp
  • A to C, chromosome 4 at 144,331,668 bp
  • T to C, chromosome 5 at 72,782,177 bp
  • T to C, chromosome 5 at 108,673,582 bp
  • A to G, chromosome 5 at 110,251,675 bp
  • A to T, chromosome 5 at 145,349,325 bp
  • A to G, chromosome 7 at 21,260,523 bp
  • G to A, chromosome 7 at 28,898,266 bp
  • A to G, chromosome 7 at 45,941,676 bp
  • A to G, chromosome 7 at 102,856,617 bp
  • C to T, chromosome 7 at 104,472,521 bp
  • A to G, chromosome 7 at 109,905,699 bp
  • G to T, chromosome 8 at 23,153,726 bp
  • A to C, chromosome 9 at 50,581,486 bp
  • A to G, chromosome 9 at 63,619,160 bp
  • T to C, chromosome 9 at 71,724,663 bp
  • A to G, chromosome 9 at 104,086,152 bp
  • C to A, chromosome 10 at 14,128,969 bp
  • A to G, chromosome 10 at 29,224,809 bp
  • T to C, chromosome 11 at 32,300,214 bp
  • G to T, chromosome 11 at 61,359,510 bp
  • C to A, chromosome 11 at 67,930,865 bp
  • A to G, chromosome 12 at 21,313,805 bp
  • T to C, chromosome 12 at 57,697,452 bp
  • A to G, chromosome 13 at 13,966,454 bp
  • A to G, chromosome 13 at 24,013,407 bp
  • A to T, chromosome 13 at 49,589,737 bp
  • A to G, chromosome 13 at 58,384,389 bp
  • A to G, chromosome 13 at 91,982,855 bp
  • T to A, chromosome 14 at 21,740,107 bp
  • G to A, chromosome 14 at 66,057,610 bp
  • T to A, chromosome 14 at 75,329,738 bp
  • A to G, chromosome 15 at 12,156,419 bp
  • A to G, chromosome 15 at 100,697,168 bp
  • T to C, chromosome 16 at 31,858,008 bp
  • G to A, chromosome 16 at 81,589,724 bp
  • T to C, chromosome 17 at 46,910,718 bp
  • G to A, chromosome 18 at 60,916,651 bp
  • A to G, chromosome 18 at 73,781,132 bp
  • C to T, chromosome 19 at 27,240,648 bp
  • G to A, chromosome 19 at 42,115,899 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3954 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040831-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.