Strain Name:
C57BL/6J-MtgxR3958Btlr/Mmmh
Stock Number:
040834-MU
Citation ID:
RRID:MMRRC_040834-MU
Other Names:
R3958 (G1), C57BL/6J-MtgxR3958Btlr
Major Collection:

Gene Information

Utrn
Name: utrophin
Synonyms: DRP, Dmdl, G-utrophin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 22288
VEGA: 10
Homologene: 21398
Espl1
Name: extra spindle pole bodies 1, separase
Synonyms: Cerp, PRCE, PRCE, separase, SSE, ESP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 105988
VEGA: 15
Homologene: 32151
Clasp1
Name: CLIP associating protein 1
Synonyms: CLASP1, 1700030C23Rik, 5730583A19Rik, B130045P17Rik, mCLASP1, CLASP1alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 76707
Homologene: 41024
Slc44a2
Name: solute carrier family 44, member 2
Synonyms: CTL2, 1110028E10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 68682
VEGA: 9
Homologene: 10711
Hcn4
Name: hyperpolarization-activated, cyclic nucleotide-gated K+ 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 330953
VEGA: 9
Homologene: 3997
Ppp6r3
Name: protein phosphatase 6, regulatory subunit 3
Synonyms: D19Bwg1430e, Saps3, 4930528G08Rik, D19Ertd703e, 9130026N02Rik, Pp6r3, Pptcs3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 52036
VEGA: 19
HGNC: HGNC:1173
Homologene: 115911
Sec23ip
Name: Sec23 interacting protein
Synonyms: D7Ertd373e, p125
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 207352
Homologene: 38288
Etl4
Name: enhancer trap locus 4
Synonyms: Sickle tail, Etl-4, 6620402G01Rik, 9430077C05Rik, Skt, E330027G05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 208618
Homologene: 10477
Panx1
Name: pannexin 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 55991
HGNC: HGNC:8599
Homologene: 49416
Snap91
Name: synaptosomal-associated protein 91
Synonyms: AP180, F1-20, 91kDa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 20616
Homologene: 8429
1110004F10Rik
Name: RIKEN cDNA 1110004F10 gene
Synonyms: sid2057
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 56372
Homologene: 8606
Ucp3
Name: uncoupling protein 3 (mitochondrial, proton carrier)
Synonyms: UCP-3, Slc25a9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 22229
Homologene: 2517
Grin1
Name: glutamate receptor, ionotropic, NMDA1 (zeta 1)
Synonyms: NR1, Nmdar, M100174, Rgsc174, NMDAR1, GluRzeta1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14810
HGNC: HGNC:4584
Homologene: 7187
Ckap4
Name: cytoskeleton-associated protein 4
Synonyms: P63, CLIMP-63, 5630400A09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 216197
VEGA: 10
Homologene: 4970
Prkg2
Name: protein kinase, cGMP-dependent, type II
Synonyms: Prkgr2, cGK-II
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 19092
HGNC: HGNC:9416
Homologene: 4556
Lrp1
Name: low density lipoprotein receptor-related protein 1
Synonyms: b2b1554Clo, CD91, A2mr
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 16971
HGNC: HGNC:6692
Homologene: 1744
Itpr2
Name: inositol 1,4,5-triphosphate receptor 2
Synonyms: Ip3r2, Itpr5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 16439
HGNC: HGNC:6181
Homologene: 37593
Ugcg
Name: UDP-glucose ceramide glucosyltransferase
Synonyms: Epcs21, GlcT-1, Ugcgl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 22234
Homologene: 37763
Neb
Name: nebulin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 17996
HGNC: HGNC:7720
Homologene: 136285
Fbxo47
Name: F-box protein 47
Synonyms: 2900052P03Rik, LOC380724
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 72973
Homologene: 47677
Rgl3
Name: ral guanine nucleotide dissociation stimulator-like 3
Synonyms: 1300003D20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 71746
VEGA: 9
Homologene: 11363
Otogl
Name: otogelin-like
Synonyms: Gm6924
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 628870
Homologene: 46008
Hoxd8
Name: homeobox D8
Synonyms: Hox-4.3, 4921540P06Rik, Hox-5.4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 15437
HGNC: HGNC:5139
Homologene: 10473
Kmt2d
Name: lysine (K)-specific methyltransferase 2D
Synonyms: C430014K11Rik, Mll2, Mll4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 381022
HGNC: HGNC:7133
Homologene: 86893
Prmt8
Name: protein arginine N-methyltransferase 8
Synonyms: Hrmt1l4, Hrmt1l3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 381813
HGNC: HGNC:5188
Homologene: 76124
Nepn
Name: nephrocan
Synonyms: Npn, 5730521E12Rik, periolin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 66650
Homologene: 23549
Cyp2s1
Name: cytochrome P450, family 2, subfamily s, polypeptide 1
Synonyms: 1200011C15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 74134
Homologene: 75274
Or4k41
Name: olfactory receptor family 4 subfamily K member 41
Synonyms: GA_x6K02T2Q125-72500603-72501520, Olfr1287, MOR248-15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 257935
Cnot6l
Name: CCR4-NOT transcription complex, subunit 6-like
Synonyms: 4932442K20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231464
Homologene: 100830
Glyat
Name: glycine-N-acyltransferase
Synonyms: A330009E03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 107146
Homologene: 64840
Actl9
Name: actin-like 9
Synonyms: 1700029I08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 69481
Homologene: 77642
Hmgcs2
Name: 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2
Synonyms: mHS
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 15360
HGNC: HGNC:5008
Homologene: 38066
Tmem106b
Name: transmembrane protein 106B
Synonyms: 2310036D22Rik, 6430519M21Rik, 5830455K21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 71900
Homologene: 56806
Celf4
Name: CUGBP, Elav-like family member 4
Synonyms: Brunol4, A230070D14Rik, BRUNOL-4, C130060B05Rik, Brul4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 108013
VEGA: 18
Homologene: 23202
Selp
Name: selectin, platelet
Synonyms: Grmp, P-selectin, CD62P
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 20344
Homologene: 2260
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 118,467,881 bp
  • T to C, chromosome 1 at 164,126,286 bp
  • A to G, chromosome 2 at 20,340,043 bp
  • G to A, chromosome 2 at 25,313,453 bp
  • T to C, chromosome 2 at 52,263,629 bp
  • C to T, chromosome 2 at 74,706,540 bp
  • G to T, chromosome 2 at 111,449,885 bp
  • T to C, chromosome 3 at 98,297,477 bp
  • C to T, chromosome 4 at 59,207,798 bp
  • A to T, chromosome 5 at 96,082,857 bp
  • G to T, chromosome 5 at 98,997,495 bp
  • A to T, chromosome 6 at 13,081,588 bp
  • G to A, chromosome 6 at 127,732,744 bp
  • C to T, chromosome 6 at 146,425,510 bp
  • C to T, chromosome 7 at 25,803,954 bp
  • A to G, chromosome 7 at 100,482,739 bp
  • A to T, chromosome 7 at 116,102,016 bp
  • C to G, chromosome 7 at 128,776,850 bp
  • GTTCTTCT to GTTCT, chromosome 9 at 15,006,171 bp
  • T to C, chromosome 9 at 21,348,541 bp
  • T to C, chromosome 9 at 21,975,589 bp
  • T to C, chromosome 9 at 58,844,048 bp
  • T to C, chromosome 9 at 86,838,130 bp
  • T to C, chromosome 10 at 12,750,108 bp
  • T to C, chromosome 10 at 52,400,708 bp
  • C to T, chromosome 10 at 84,528,164 bp
  • T to C, chromosome 10 at 107,821,925 bp
  • A to G, chromosome 10 at 127,571,958 bp
  • A to G, chromosome 11 at 97,878,139 bp
  • G to A, chromosome 15 at 98,855,549 bp
  • A to G, chromosome 15 at 102,312,989 bp
  • A to G, chromosome 17 at 33,433,764 bp
  • A to G, chromosome 18 at 25,537,754 bp
  • A to T, chromosome 19 at 3,496,583 bp
  • A to T, chromosome 19 at 12,639,833 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3958 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040834-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.