Strain Name:
C57BL/6J-MtgxR4304Btlr/Mmmh
Stock Number:
040865-MU
Citation ID:
RRID:MMRRC_040865-MU
Other Names:
R4304 (G1), C57BL/6J-MtgxR4304Btlr
Major Collection:

Gene Information

Map4k4
Name: mitogen-activated protein kinase kinase kinase kinase 4
Synonyms: Nik, 9430080K19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 26921
HGNC: HGNC:6866
Homologene: 7442
Resf1
Name: retroelement silencing factor 1
Synonyms: GET, 2810474O19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 67246
Homologene: 19251
Arhgap42
Name: Rho GTPase activating protein 42
Synonyms: 9030420J04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 71544
Homologene: 28446
Gcfc2
Name: GC-rich sequence DNA binding factor 2
Synonyms: AW146020
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 330361
HGNC: HGNC:1317
Homologene: 2411
Rad23a
Name: RAD23 homolog A, nucleotide excision repair protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 19358
HGNC: HGNC:9812
Homologene: 48322
Npc1
Name: NPC intracellular cholesterol transporter 1
Synonyms: D18Ertd723e, D18Ertd139e, C85354, lcsd, A430089E03Rik, nmf164
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 18145
HGNC: HGNC:7897
Homologene: 228
Adam32
Name: a disintegrin and metallopeptidase domain 32
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 353188
Homologene: 17021
Csgalnact1
Name: chondroitin sulfate N-acetylgalactosaminyltransferase 1
Synonyms: CSGalNAcT-1, 4732435N03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234356
Homologene: 23099
Fam3d
Name: FAM3 metabolism regulating signaling molecule D
Synonyms: EF-7, 2310076N21Rik, Oit1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 18300
VEGA: 14
Homologene: 16354
Cep152
Name: centrosomal protein 152
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 99100
Homologene: 37159
Fig4
Name: FIG4 phosphoinositide 5-phosphatase
Synonyms: A530089I17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 103199
VEGA: 10
Homologene: 6713
Snx13
Name: sorting nexin 13
Synonyms: RGS-PX1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 217463
VEGA: 12
Homologene: 41011
Slc4a11
Name: solute carrier family 4, sodium bicarbonate transporter-like, member 11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 269356
Homologene: 12931
Wnk2
Name: WNK lysine deficient protein kinase 2
Synonyms: ESTM15, 1810073P09Rik, X83337
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 75607
Homologene: 19155
Arhgef5
Name: Rho guanine nucleotide exchange factor (GEF) 5
Synonyms: 2210412D05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 54324
Homologene: 66300
Frmd4a
Name: FERM domain containing 4A
Synonyms: C230040M21Rik, 2700017I06Rik, Gm13190
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 209630
Homologene: 9971
Nags
Name: N-acetylglutamate synthase
Synonyms: 1700120E20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217214
Homologene: 77363
Olfr1240
Name: olfactory receptor 1240
Synonyms: GA_x6K02T2Q125-50883183-50882239, MOR231-8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258804
Homologene: 121591
Vmn2r12
Name: vomeronasal 2, receptor 12
Synonyms: Gm6769
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 627569
Homologene: 129606
Ganc
Name: glucosidase, alpha; neutral C
Synonyms: 5830445O15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 76051
HGNC: HGNC:4139
Homologene: 25627
Prr27
Name: proline rich 27
Synonyms: 4930432K09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 73779
Homologene: 136277
Cfap157
Name: cilia and flagella associated protein 157
Synonyms: 1700019L03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 227736
Homologene: 53056
Gm5592
Name: predicted gene 5592
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 434172
Homologene: 45597
Tpp1
Name: tripeptidyl peptidase I
Synonyms: Cln2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 12751
HGNC: HGNC:2073
Homologene: 335
Rptn
Name: repetin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 20129
Homologene: 84780
Vmn1r238
Name: vomeronasal 1 receptor, 238
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 100312476
Homologene: 128342
H2-M3
Name: histocompatibility 2, M region locus 3
Synonyms: Hmt, H-2M3, R4B2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 14991
HGNC: HGNC:4964
Homologene: 133255
Gm7173
Name: predicted gene 7173
Synonyms:
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Tex11
Name: testis expressed gene 11
Synonyms: 4930565P14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 83558
Homologene: 49962
RP24-388P13.2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Wfdc15b
Name: WAP four-disulfide core domain 15B
Synonyms: Swam1, 9230106L14Rik, Wfdc15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 192201
Homologene: 77219
Gm20939
Name: predicted gene, 20939
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 100044193
VEGA: 17
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 39,973,972 bp
  • A to G, chromosome 1 at 64,736,574 bp
  • C to T, chromosome 2 at 4,333,078 bp
  • G to A, chromosome 2 at 32,779,042 bp
  • A to T, chromosome 2 at 89,440,198 bp
  • A to G, chromosome 2 at 120,433,806 bp
  • G to A, chromosome 2 at 125,563,723 bp
  • A to T, chromosome 2 at 130,688,138 bp
  • A to C, chromosome 2 at 164,215,468 bp
  • C to A, chromosome 3 at 93,396,931 bp
  • A to G, chromosome 5 at 87,842,907 bp
  • A to G, chromosome 5 at 109,086,006 bp
  • C to T, chromosome 6 at 43,279,498 bp
  • G to A, chromosome 6 at 81,943,007 bp
  • C to T, chromosome 6 at 149,326,238 bp
  • A to T, chromosome 7 at 41,286,262 bp
  • T to A, chromosome 7 at 105,750,309 bp
  • A to T, chromosome 8 at 24,901,529 bp
  • A to T, chromosome 8 at 68,372,642 bp
  • A to G, chromosome 8 at 84,838,679 bp
  • A to G, chromosome 9 at 9,006,488 bp
  • T to A, chromosome 10 at 41,256,427 bp
  • C to T, chromosome 11 at 102,146,920 bp
  • A to G, chromosome 12 at 35,122,942 bp
  • C to T, chromosome 13 at 49,090,837 bp
  • G to A, chromosome 14 at 8,349,324 bp
  • T to C, chromosome 17 at 37,272,404 bp
  • A to C, chromosome 17 at 94,877,281 bp
  • T to A, chromosome 18 at 3,123,040 bp
  • C to T, chromosome 18 at 12,210,527 bp
  • C to G, chromosome X at 79,498,029 bp
  • C to A, chromosome X at 100,933,415 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4304 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040865-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.