Strain Name:
C57BL/6J-MtgxR4353Btlr/Mmmh
Stock Number:
040866-MU
Citation ID:
RRID:MMRRC_040866-MU
Other Names:
R4353 (G1), C57BL/6J-MtgxR4353Btlr
Major Collection:

Gene Information

Myo5a
Name: myosin VA
Synonyms: flail, MyoVA, 9630007J19Rik, Myo5, Dbv, MVa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 17918
HGNC: HGNC:7602
Homologene: 20100
Nrp2
Name: neuropilin 2
Synonyms: NP2, NP-2, Npn2, Npn-2, 1110048P06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 18187
HGNC: HGNC:8005
Homologene: 2875
Ppp2r2a
Name: protein phosphatase 2, regulatory subunit B, alpha
Synonyms: 2410004D02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 71978
VEGA: 14
HGNC: HGNC:9304
Homologene: 2035
Ptch1
Name: patched 1
Synonyms: Patched 1, wig, Ptc1, Ptc, A230106A15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 19206
HGNC: HGNC:9585
Homologene: 223
Etv1
Name: ets variant 1
Synonyms: ER81, Etsrp81
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 14009
HGNC: HGNC:3490
Homologene: 3636
Rnf38
Name: ring finger protein 38
Synonyms: Oip1, 1700065B19Rik, 2610202O07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 73469
Homologene: 32550
Vps54
Name: VPS54 GARP complex subunit
Synonyms: wr, Vps54l, 5330404P15Rik, mSLP8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 245944
Homologene: 5605
Sema4b
Name: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B
Synonyms: Semac, SemC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 20352
Homologene: 8426
Zfp638
Name: zinc finger protein 638
Synonyms: Np220, Zfml
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 18139
Homologene: 7447
Elovl5
Name: ELOVL family member 5, elongation of long chain fatty acids (yeast)
Synonyms: 1110059L23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 68801
Homologene: 41449
Dhx9
Name: DEAH (Asp-Glu-Ala-His) box polypeptide 9
Synonyms: NDHII, RNA helicase, RHA, nuclear DNA helicase II, NDH II, leukophysin, Ddx9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 13211
HGNC: HGNC:2750
Homologene: 1039
Zc3h14
Name: zinc finger CCCH type containing 14
Synonyms: 2700069A02Rik, 1700016A15Rik, 1010001P15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 75553
VEGA: 12
Homologene: 32605
Bzw2
Name: basic leucine zipper and W2 domains 2
Synonyms: HSPC028, 1110001I24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 66912
VEGA: 12
Homologene: 8530
Pabpc4
Name: poly(A) binding protein, cytoplasmic 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 230721
HGNC: HGNC:8557
Homologene: 37855
Iqgap2
Name: IQ motif containing GTPase activating protein 2
Synonyms: 4933417J23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 544963
VEGA: 13
HGNC: HGNC:6111
Homologene: 101543
Nup214
Name: nucleoporin 214
Synonyms: CAN, D2H9S46E
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 227720
HGNC: HGNC:8064
Homologene: 38008
Clmp
Name: CXADR-like membrane protein
Synonyms: 9030425E11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 71566
Homologene: 11705
Sox8
Name: SRY (sex determining region Y)-box 8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 20681
Homologene: 7950
Dcdc2a
Name: doublecortin domain containing 2a
Synonyms: RU2, Dcdc2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 195208
Homologene: 9483
Spg11
Name: SPG11, spatacsin vesicle trafficking associated
Synonyms: 6030465E24Rik, spastic paraplegia 11, C530005A01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 214585
Homologene: 41614
Arhgap33
Name: Rho GTPase activating protein 33
Synonyms: NOMA-GAP, Tcgap, Snx26
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 233071
Homologene: 76448
Slc12a7
Name: solute carrier family 12, member 7
Synonyms: Kcc4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 20499
VEGA: 13
Homologene: 21312
Inava
Name: innate immunity activator
Synonyms: 5730559C18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 67313
Homologene: 10103
Atp2b2
Name: ATPase, Ca++ transporting, plasma membrane 2
Synonyms: jog, D6Abb2e, PMCA2, wms, Gena300, Tmy
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 11941
HGNC: HGNC:815
Homologene: 56150
Mecom
Name: MDS1 and EVI1 complex locus
Synonyms: Evi1, MDS1-EVI1, Jbo, Prdm3, D630039M04Rik, Evi-1, Mds1, ZNFPR1B1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 14013
HGNC: HGNC:3498
Homologene: 21086
Scn7a
Name: sodium channel, voltage-gated, type VII, alpha
Synonyms: 1110034K09Rik, Scn6a, Nax, Nav2.3, NaG, Nav2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 20272
Homologene: 55706
Col27a1
Name: collagen, type XXVII, alpha 1
Synonyms: 5730512J02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 373864
Homologene: 69400
Poln
Name: DNA polymerase N
Synonyms: POL4P
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 272158
Homologene: 131253
A2ml1
Name: alpha-2-macroglobulin like 1
Synonyms: Ovos2, BC048546
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 232400
Homologene: 45969
Heg1
Name: heart development protein with EGF-like domains 1
Synonyms: 9530025L16Rik, 5530401I02Rik, LOC268884, 4632417D23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 77446
Homologene: 35276
Dthd1
Name: death domain containing 1
Synonyms: Gm17384
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 100322896
Homologene: 123510
Alpk2
Name: alpha-kinase 2
Synonyms: Hak
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 225638
Homologene: 50475
Cpb1
Name: carboxypeptidase B1 (tissue)
Synonyms: 2210008M23Rik, 0910001A18Rik, 1810063F02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 76703
HGNC: HGNC:2299
Homologene: 68127
Pcnx2
Name: pecanex homolog 2
Synonyms: Pcnxl2, E330039K12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 270109
HGNC: HGNC:8736
Homologene: 8987
Nlrp4d
Name: NLR family, pyrin domain containing 4D
Synonyms: Nalp4d, Nalp-beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 384752
B4galnt3
Name: beta-1,4-N-acetyl-galactosaminyl transferase 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 330406
Homologene: 18328
Cyp2c37
Name: cytochrome P450, family 2. subfamily c, polypeptide 37
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 13096
Homologene: 137231
Rnf130
Name: ring finger protein 130
Synonyms: 2510042A13Rik, G1RP, G1RZFP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 59044
Homologene: 41267
Cttnbp2
Name: cortactin binding protein 2
Synonyms: ORF4, 3010022N24Rik, Cortbp2, 4732477G22Rik, 9130022E09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 30785
Homologene: 14125
Cdh20
Name: cadherin 20
Synonyms: Cdh7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 23836
HGNC: HGNC:1760
Homologene: 8015
Atp8a1
Name: ATPase, aminophospholipid transporter (APLT), class I, type 8A, member 1
Synonyms: B230107D19Rik, Atp3a2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 11980
Homologene: 48402
Tmbim7
Name: transmembrane BAX inhibitor motif containing 7
Synonyms: Lfg5, 4930500J03Rik, 4930511M11Rik, 4930403J02Rik, Tmbim1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 75010
Homologene: 81927
Llgl1
Name: LLGL1 scribble cell polarity complex component
Synonyms: Lgl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 16897
HGNC: HGNC:6628
Homologene: 31220
Rnf31
Name: ring finger protein 31
Synonyms: Paul, HOIP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 268749
Homologene: 33228
Dsc2
Name: desmocollin 2
Synonyms: Dsc2b, Dsc2a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 13506
HGNC: HGNC:3036
Homologene: 8397
Prb1
Name: proline-rich protein BstNI subfamily 1
Synonyms: proline-rich proteoglycan 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 381833
HGNC: HGNC:9338
Stk36
Name: serine/threonine kinase 36
Synonyms: Fused, 1700112N14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 269209
Homologene: 49432
Gm16332
Name: predicted gene 16332
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 100417200
Olfr93
Name: olfactory receptor 93
Synonyms: GA_x6K02T2PSCP-1592036-1591098, MOR256-39P
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 258051
HGNC: HGNC:8252
Homologene: 79436
Coq6
Name: coenzyme Q6 monooxygenase
Synonyms: 5930427M12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 217707
Homologene: 6039
Vmn1r6
Name: vomeronasal 1 receptor 6
Synonyms: V1rc20
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 171193
Homologene: 128340
Nt5el
Name: 5' nucleotidase, ecto-like
Synonyms: 4933425L06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 66763
Homologene: 12028
Kyat3
Name: kynurenine aminotransferase 3
Synonyms: Kat3, Ccbl2, KATIII
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 229905
Homologene: 2994
Gem
Name: GTP binding protein (gene overexpressed in skeletal muscle)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 14579
HGNC: HGNC:4234
Homologene: 38024
Dusp2
Name: dual specificity phosphatase 2
Synonyms: PAC1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 13537
HGNC: HGNC:3068
Homologene: 3255
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 62,738,417 bp
  • A to G, chromosome 1 at 74,632,807 bp
  • A to G, chromosome 1 at 104,979,089 bp
  • C to T, chromosome 1 at 136,226,208 bp
  • A to T, chromosome 1 at 139,930,808 bp
  • A to G, chromosome 1 at 153,471,789 bp
  • T to C, chromosome 2 at 31,977,917 bp
  • T to A, chromosome 2 at 66,676,436 bp
  • G to T, chromosome 2 at 122,113,194 bp
  • A to G, chromosome 2 at 127,337,336 bp
  • T to C, chromosome 3 at 20,262,544 bp
  • C to A, chromosome 3 at 29,966,738 bp
  • T to C, chromosome 3 at 142,731,293 bp
  • G to A, chromosome 4 at 11,705,939 bp
  • A to T, chromosome 4 at 44,149,100 bp
  • G to T, chromosome 4 at 63,225,631 bp
  • C to T, chromosome 4 at 123,290,267 bp
  • A to G, chromosome 5 at 3,661,796 bp
  • C to T, chromosome 5 at 34,129,452 bp
  • T to A, chromosome 5 at 62,842,867 bp
  • T to C, chromosome 5 at 67,769,108 bp
  • T to C, chromosome 6 at 18,514,704 bp
  • C to T, chromosome 6 at 57,002,692 bp
  • T to C, chromosome 6 at 83,984,059 bp
  • T to C, chromosome 6 at 113,765,784 bp
  • T to C, chromosome 6 at 120,215,476 bp
  • C to T, chromosome 6 at 128,580,386 bp
  • T to G, chromosome 6 at 132,313,661 bp
  • T to A, chromosome 7 at 10,381,841 bp
  • C to A, chromosome 7 at 30,524,136 bp
  • C to A, chromosome 7 at 80,215,651 bp
  • T to C, chromosome 8 at 125,762,851 bp
  • T to C, chromosome 9 at 40,686,322 bp
  • T to C, chromosome 9 at 75,211,071 bp
  • T to C, chromosome 9 at 77,960,917 bp
  • C to T, chromosome 11 at 21,316,851 bp
  • T to C, chromosome 11 at 50,087,440 bp
  • C to T, chromosome 11 at 60,709,568 bp
  • A to T, chromosome 12 at 36,123,979 bp
  • A to G, chromosome 12 at 38,857,106 bp
  • G to A, chromosome 12 at 84,368,149 bp
  • A to G, chromosome 12 at 98,763,960 bp
  • T to A, chromosome 13 at 25,056,491 bp
  • C to T, chromosome 13 at 63,534,329 bp
  • C to T, chromosome 13 at 73,790,734 bp
  • C to T, chromosome 13 at 95,671,396 bp
  • T to C, chromosome 13 at 105,118,745 bp
  • AAC to A, chromosome 14 at 55,601,098 bp
  • T to A, chromosome 14 at 67,028,937 bp
  • A to G, chromosome 16 at 33,710,477 bp
  • A to C, chromosome 17 at 25,567,335 bp
  • T to A, chromosome 17 at 37,151,337 bp
  • G to T, chromosome 18 at 20,050,068 bp
  • C to T, chromosome 18 at 65,291,452 bp
  • A to G, chromosome 19 at 40,000,545 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4353 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040866-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.