Strain Name:
C57BL/6J-MtgxR1501Btlr/Mmmh
Stock Number:
040867-MU
Citation ID:
RRID:MMRRC_040867-MU
Other Names:
R1501 (G1), C57BL/6J-MtgxR1501Btlr
Major Collection:

Gene Information

Gene Symbol: Mc3r [MGI:96929] (Mus musculus (mouse))
Name: melanocortin 3 receptor
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Upp1 [MGI:1097668] (Mus musculus (mouse))
Name: uridine phosphorylase 1
Synonyms: UdRPase, UPase, Up
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Dlc1 [MGI:1354949] (Mus musculus (mouse))
Name: deleted in liver cancer 1
Synonyms: A730069N07Rik, p122-RhoGAP, STARD12, Arhgap7
Chromosome: 8
Alteration at locus: Chemically Induced
Gene Symbol: Srgap2 [MGI:109605] (Mus musculus (mouse))
Name: SLIT-ROBO Rho GTPase activating protein 2
Synonyms: FBP2, 9930124L22Rik, Fnbp2
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Ddx10 [MGI:1924841] (Mus musculus (mouse))
Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 10
Synonyms: 4632415A01Rik
Chromosome: 9
Alteration at locus: Chemically Induced
Gene Symbol: Plekhm1 [MGI:2443207] (Mus musculus (mouse))
Name: pleckstrin homology domain containing, family M (with RUN domain) member 1
Synonyms: B2, AP162, D330036J23Rik
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Ptpn5 [MGI:97807] (Mus musculus (mouse))
Name: protein tyrosine phosphatase, non-receptor type 5
Synonyms: Step
Chromosome: 7
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 65,265,284 bp
  • G to A, chromosome 1 at 127,397,641 bp
  • A to G, chromosome 1 at 131,292,699 bp
  • A to G, chromosome 1 at 175,975,521 bp
  • T to G, chromosome 2 at 19,201,828 bp
  • T to A, chromosome 2 at 26,440,045 bp
  • A to G, chromosome 2 at 66,700,163 bp
  • A to G, chromosome 2 at 69,158,785 bp
  • A to T, chromosome 2 at 71,081,988 bp
  • A to G, chromosome 2 at 82,235,799 bp
  • G to A, chromosome 2 at 88,592,148 bp
  • T to G, chromosome 2 at 172,249,380 bp
  • C to T, chromosome 3 at 36,552,755 bp
  • T to C, chromosome 3 at 59,260,835 bp
  • T to C, chromosome 3 at 87,090,472 bp
  • T to A, chromosome 3 at 90,225,503 bp
  • T to C, chromosome 3 at 152,237,686 bp
  • A to G, chromosome 4 at 32,562,279 bp
  • T to A, chromosome 4 at 96,215,444 bp
  • C to T, chromosome 4 at 141,964,625 bp
  • C to T, chromosome 4 at 147,870,138 bp
  • T to A, chromosome 5 at 3,643,701 bp
  • A to G, chromosome 5 at 30,128,806 bp
  • T to C, chromosome 5 at 34,555,576 bp
  • T to C, chromosome 5 at 103,516,364 bp
  • G to T, chromosome 5 at 137,911,630 bp
  • A to G, chromosome 6 at 34,955,122 bp
  • A to G, chromosome 6 at 40,950,433 bp
  • C to T, chromosome 6 at 88,376,200 bp
  • A to T, chromosome 6 at 89,976,216 bp
  • T to C, chromosome 6 at 125,110,152 bp
  • T to C, chromosome 6 at 139,844,070 bp
  • G to T, chromosome 7 at 47,089,875 bp
  • A to T, chromosome 7 at 64,389,504 bp
  • A to T, chromosome 7 at 86,165,642 bp
  • A to G, chromosome 7 at 89,633,065 bp
  • T to A, chromosome 7 at 104,340,888 bp
  • G to A, chromosome 7 at 105,668,463 bp
  • A to C, chromosome 7 at 108,396,082 bp
  • G to A, chromosome 7 at 108,544,575 bp
  • T to A, chromosome 7 at 123,260,508 bp
  • T to A, chromosome 7 at 132,269,069 bp
  • A to C, chromosome 8 at 24,707,499 bp
  • G to T, chromosome 8 at 36,938,148 bp
  • A to G, chromosome 8 at 48,343,316 bp
  • G to A, chromosome 8 at 110,221,603 bp
  • A to G, chromosome 9 at 8,610,169 bp
  • T to C, chromosome 9 at 53,233,997 bp
  • A to G, chromosome 9 at 62,871,340 bp
  • C to A, chromosome 10 at 3,957,361 bp
  • T to C, chromosome 10 at 76,039,769 bp
  • G to A, chromosome 10 at 82,954,635 bp
  • T to A, chromosome 11 at 9,134,708 bp
  • A to G, chromosome 11 at 44,632,743 bp
  • G to T, chromosome 11 at 53,688,151 bp
  • G to T, chromosome 11 at 60,464,853 bp
  • G to A, chromosome 11 at 71,156,059 bp
  • A to G, chromosome 11 at 83,003,180 bp
  • A to G, chromosome 11 at 85,834,796 bp
  • A to T, chromosome 11 at 94,285,981 bp
  • A to G, chromosome 11 at 103,387,062 bp
  • T to C, chromosome 11 at 115,604,197 bp
  • T to C, chromosome 12 at 116,430,092 bp
  • C to T, chromosome 13 at 19,104,291 bp
  • T to C, chromosome 13 at 30,935,502 bp
  • A to G, chromosome 13 at 69,631,587 bp
  • C to A, chromosome 13 at 73,684,048 bp
  • A to G, chromosome 14 at 28,981,180 bp
  • A to G, chromosome 14 at 50,187,082 bp
  • A to T, chromosome 14 at 78,513,347 bp
  • T to C, chromosome 15 at 34,510,357 bp
  • C to A, chromosome 15 at 54,839,514 bp
  • C to A, chromosome 15 at 82,454,324 bp
  • C to A, chromosome 15 at 101,778,334 bp
  • C to T, chromosome 16 at 20,737,533 bp
  • T to C, chromosome 16 at 45,777,783 bp
  • G to A, chromosome 17 at 12,507,104 bp
  • T to A, chromosome 17 at 18,439,856 bp
  • T to G, chromosome 17 at 19,362,259 bp
  • T to C, chromosome 17 at 28,638,562 bp
  • T to C, chromosome 17 at 71,310,761 bp
  • A to G, chromosome 17 at 71,365,094 bp
  • A to T, chromosome 18 at 5,761,399 bp
  • A to T, chromosome 18 at 20,332,019 bp
  • G to A, chromosome 18 at 77,356,832 bp
  • A to G, chromosome 19 at 5,716,424 bp
  • T to C, chromosome 19 at 34,588,251 bp
  • A to G, chromosome 19 at 43,829,927 bp
  • T to C, chromosome 19 at 45,778,640 bp
  • G to T, chromosome X at 87,304,863 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1501 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040867-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.