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Strain Name:
C57BL/6J-MtgxR3937Btlr/Mmmh
Stock Number:
040921-MU
Citation ID:
RRID:MMRRC_040921-MU
Other Names:
R3937 (G1), C57BL/6J-MtgxR3937Btlr
Major Collection:

Strain Information

Utrn
Name: utrophin
Synonyms: G-utrophin, Dmdl, DRP
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 22288
VEGA: 10
Homologene: 21398
Plekhj1
Name: pleckstrin homology domain containing, family J member 1
Synonyms: 9530063M10Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 78670
VEGA: 10
Homologene: 11389
Sema6d
Name: sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D
Synonyms: 1110067B02Rik, Sema6D-6, Sema6D-5, Sema6D-4, Sema6D-2, Sema6D-1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 214968
Homologene: 16195
Elavl3
Name: ELAV like RNA binding protein 3
Synonyms: mHuC, Huc, 2600009P04Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 15571
VEGA: 9
HGNC: HGNC:3314
Homologene: 31035
Nes
Name: nestin
Synonyms: RC2, Marc2, ESTM46, Ifaprc2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18008
HGNC: HGNC:7756
Homologene: 136487
Smarcad1
Name: SNF2 related chromatin remodeling ATPase with DExD box 1
Synonyms: D6Pas1, Etl1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 13990
Homologene: 5301
Hspa4
Name: heat shock protein 4
Synonyms: APG-2, Hsp70RY, Hsp110, 70kDa
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 15525
HGNC: HGNC:5237
Homologene: 1624
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 63,737,836 bp
  • A to T, chromosome 1 at 75,477,540 bp
  • A to G, chromosome 1 at 88,258,664 bp
  • A to T, chromosome 1 at 130,804,576 bp
  • C to T, chromosome 2 at 24,087,139 bp
  • A to G, chromosome 2 at 25,610,639 bp
  • A to G, chromosome 2 at 32,579,112 bp
  • T to A, chromosome 2 at 86,298,016 bp
  • T to C, chromosome 2 at 103,022,219 bp
  • A to T, chromosome 2 at 124,656,850 bp
  • A to T, chromosome 2 at 126,127,060 bp
  • A to G, chromosome 2 at 158,200,360 bp
  • G to T, chromosome 2 at 164,910,700 bp
  • C to T, chromosome 3 at 19,971,034 bp
  • A to T, chromosome 3 at 87,971,236 bp
  • G to A, chromosome 3 at 90,403,987 bp
  • A to G, chromosome 3 at 157,920,040 bp
  • T to C, chromosome 4 at 109,757,692 bp
  • T to C, chromosome 4 at 116,998,530 bp
  • T to C, chromosome 4 at 118,934,683 bp
  • T to C, chromosome 4 at 131,774,304 bp
  • T to A, chromosome 4 at 136,255,143 bp
  • T to C, chromosome 4 at 141,840,321 bp
  • T to G, chromosome 4 at 147,942,053 bp
  • T to C, chromosome 4 at 148,654,956 bp
  • A to T, chromosome 5 at 101,944,239 bp
  • A to T, chromosome 6 at 14,719,074 bp
  • T to A, chromosome 6 at 42,744,076 bp
  • T to G, chromosome 6 at 65,114,336 bp
  • T to A, chromosome 6 at 66,679,073 bp
  • T to C, chromosome 6 at 136,840,455 bp
  • A to G, chromosome 7 at 41,428,130 bp
  • A to G, chromosome 9 at 22,018,744 bp
  • A to T, chromosome 9 at 99,336,192 bp
  • T to C, chromosome 10 at 12,709,955 bp
  • T to C, chromosome 10 at 58,476,472 bp
  • A to T, chromosome 10 at 76,286,500 bp
  • A to G, chromosome 10 at 80,797,775 bp
  • T to A, chromosome 11 at 53,270,949 bp
  • C to T, chromosome 11 at 76,212,888 bp
  • T to C, chromosome 11 at 77,515,938 bp
  • G to T, chromosome 11 at 109,974,567 bp
  • T to C, chromosome 11 at 116,841,165 bp
  • C to T, chromosome 11 at 117,723,529 bp
  • C to A, chromosome 12 at 67,221,206 bp
  • A to T, chromosome 13 at 22,883,188 bp
  • A to T, chromosome 13 at 36,916,901 bp
  • T to C, chromosome 14 at 31,634,191 bp
  • T to A, chromosome 14 at 53,538,323 bp
  • T to C, chromosome 14 at 54,963,055 bp
  • T to A, chromosome 14 at 123,369,945 bp
  • A to G, chromosome 15 at 39,437,845 bp
  • G to A, chromosome 15 at 76,536,624 bp
  • T to C, chromosome 15 at 79,734,155 bp
  • C to A, chromosome 15 at 91,778,504 bp
  • C to T, chromosome 16 at 85,795,619 bp
  • A to G, chromosome 17 at 37,672,967 bp
  • A to T, chromosome 18 at 21,148,806 bp
  • A to G, chromosome 18 at 25,090,761 bp
  • A to G, chromosome 18 at 67,135,370 bp
  • T to C, chromosome 18 at 74,716,037 bp
  • A to G, chromosome 19 at 46,004,053 bp
  • C to T, chromosome X at 70,462,186 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3937 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040921-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.