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Strain Name:
C57BL/6J-MtgxR3950Btlr/Mmmh
Stock Number:
040930-MU
Citation ID:
RRID:MMRRC_040930-MU
Other Names:
R3950 (G1), C57BL/6J-MtgxR3950Btlr
Major Collection:

Strain Information

Epha4
Name: Eph receptor A4
Synonyms: Sek, Sek1, Tyro1, Hek8, Cek8, 2900005C20Rik, rb
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13838
HGNC: HGNC:3388
Homologene: 20933
Ptch1
Name: patched 1
Synonyms: Ptc, Patched 1, Ptc1, A230106A15Rik, wig
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 19206
HGNC: HGNC:9585
Homologene: 223
Thop1
Name: thimet oligopeptidase 1
Synonyms: EP24.15
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 50492
Homologene: 55726
Trip10
Name: thyroid hormone receptor interactor 10
Synonyms: Cip4
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 106628
VEGA: 17
Homologene: 99728
Ticrr
Name: TOPBP1-interacting checkpoint and replication regulator
Synonyms: 5730590G19Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 77011
Homologene: 67120
Appbp2
Name: amyloid beta precursor protein binding protein 2
Synonyms: PAT1, 1300003O07Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 66884
HGNC: HGNC:622
Homologene: 31378
Srek1
Name: splicing regulatory glutamine/lysine-rich protein 1
Synonyms: SRrp508, SRrp86, Sfrs12, AL118220
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 218543
Homologene: 10581
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 66,622,570 bp
  • G to A, chromosome 1 at 74,079,195 bp
  • A to T, chromosome 1 at 77,399,716 bp
  • T to C, chromosome 2 at 68,664,403 bp
  • T to G, chromosome 2 at 72,438,300 bp
  • T to C, chromosome 2 at 87,545,065 bp
  • A to G, chromosome 2 at 103,035,241 bp
  • T to C, chromosome 2 at 127,248,657 bp
  • A to T, chromosome 2 at 150,952,488 bp
  • G to A, chromosome 2 at 155,709,083 bp
  • C to T, chromosome 2 at 168,099,194 bp
  • A to G, chromosome 2 at 174,121,113 bp
  • C to T, chromosome 3 at 98,856,138 bp
  • T to C, chromosome 3 at 114,121,445 bp
  • T to C, chromosome 4 at 98,737,353 bp
  • T to A, chromosome 6 at 23,247,420 bp
  • T to A, chromosome 6 at 121,878,530 bp
  • A to G, chromosome 6 at 137,837,120 bp
  • T to C, chromosome 7 at 12,925,722 bp
  • T to A, chromosome 7 at 13,261,577 bp
  • T to C, chromosome 7 at 79,682,069 bp
  • T to C, chromosome 7 at 130,467,292 bp
  • A to T, chromosome 9 at 7,112,061 bp
  • G to A, chromosome 9 at 15,998,271 bp
  • C to T, chromosome 9 at 44,385,227 bp
  • C to A, chromosome 9 at 65,591,834 bp
  • G to T, chromosome 9 at 72,296,019 bp
  • A to G, chromosome 9 at 96,687,546 bp
  • C to T, chromosome 9 at 102,542,428 bp
  • T to A, chromosome 9 at 110,428,902 bp
  • T to A, chromosome 10 at 43,909,897 bp
  • C to T, chromosome 10 at 52,066,388 bp
  • A to G, chromosome 10 at 59,164,953 bp
  • T to C, chromosome 10 at 60,657,326 bp
  • C to T, chromosome 10 at 81,080,629 bp
  • A to T, chromosome 10 at 96,042,134 bp
  • T to C, chromosome 10 at 100,615,296 bp
  • T to C, chromosome 10 at 127,185,144 bp
  • A to G, chromosome 11 at 67,700,051 bp
  • G to T, chromosome 11 at 69,405,615 bp
  • A to G, chromosome 11 at 73,136,395 bp
  • C to T, chromosome 11 at 83,989,815 bp
  • A to G, chromosome 11 at 85,194,706 bp
  • A to G, chromosome 11 at 89,987,318 bp
  • A to G, chromosome 12 at 57,649,506 bp
  • A to T, chromosome 12 at 112,785,789 bp
  • T to C, chromosome 12 at 113,061,113 bp
  • A to T, chromosome 13 at 48,589,194 bp
  • T to G, chromosome 13 at 63,524,959 bp
  • G to A, chromosome 13 at 103,744,895 bp
  • A to G, chromosome 14 at 73,262,662 bp
  • T to C, chromosome 15 at 32,689,338 bp
  • T to C, chromosome 15 at 66,469,806 bp
  • A to G, chromosome 15 at 71,977,358 bp
  • G to T, chromosome 16 at 38,768,022 bp
  • T to A, chromosome 16 at 79,073,186 bp
  • C to A, chromosome 17 at 26,197,616 bp
  • T to C, chromosome 17 at 47,688,582 bp
  • G to A, chromosome 17 at 55,995,517 bp
  • T to C, chromosome 17 at 57,225,286 bp
  • T to A, chromosome 17 at 57,253,411 bp
  • A to G, chromosome 18 at 13,846,346 bp
  • C to G, chromosome 18 at 34,614,444 bp
  • T to C, chromosome 18 at 60,697,139 bp
  • A to G, chromosome 19 at 4,343,232 bp
  • C to T, chromosome 19 at 4,617,967 bp
  • C to T, chromosome 19 at 43,664,269 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3950 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040930-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.