Strain Name:
C57BL/6J-MtgxR4003Btlr/Mmmh
Stock Number:
040945-MU
Citation ID:
RRID:MMRRC_040945-MU
Other Names:
R4003 (G1), C57BL/6J-MtgxR4003Btlr
Major Collection:

Strain Information

Gsn
Name: gelsolin
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227753
HGNC: HGNC:4620
Homologene: 147
Zfp384
Name: zinc finger protein 384
Synonyms: C130073D16Rik, Ciz, Nmp4
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 269800
Homologene: 15849
Smc2
Name: structural maintenance of chromosomes 2
Synonyms: CAP-E, Fin16, 5730502P04Rik, Smc2l1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 14211
Homologene: 4705
Rabep1
Name: rabaptin, RAB GTPase binding effector protein 1
Synonyms: RAB5 effector protein, neurocrescin, rabaptin-5
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 54189
Homologene: 3451
Csnk2a1
Name: casein kinase 2, alpha 1 polypeptide
Synonyms: Csnk2a1-rs4, CK2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 12995
Homologene: 90874
Triobp
Name: TRIO and F-actin binding protein
Synonyms: Mus EST 478828, Tara, EST478828
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 110253
Homologene: 5104
Chd9
Name: chromodomain helicase DNA binding protein 9
Synonyms: 1810014J18Rik, AD013, A330063D19Rik, 9030205D12Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 109151
Homologene: 11844
Zfp619
Name: zinc finger protein 619
Synonyms: 3000002G13Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 70227
Homologene: 133818
Myo1b
Name: myosin IB
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 17912
HGNC: HGNC:7596
Homologene: 7856
Eftud2
Name: elongation factor Tu GTP binding domain containing 2
Synonyms: U5-116kD, 116kDa, Snrp116
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20624
Homologene: 3133
Fam222b
Name: family with sequence similarity 222, member B
Synonyms: BC017647
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216971
Homologene: 10054
Scn10a
Name: sodium channel, voltage-gated, type X, alpha
Synonyms: Nav1.8
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 20264
VEGA: 9
Homologene: 21300
Dpy19l2
Name: dpy-19 like 2
Synonyms: 4932443J21Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 320752
VEGA: 9
Homologene: 77569
Map2
Name: microtubule-associated protein 2
Synonyms: MAP-2, G1-397-34, Mtap2, repro4
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 17756
HGNC: HGNC:6839
Homologene: 1779
Zfp236
Name: zinc finger protein 236
Synonyms: LOC240456
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 329002
Homologene: 7198
Tmco3
Name: transmembrane and coiled-coil domains 3
Synonyms: B230339H12Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234076
Homologene: 32376
Tnfrsf25
Name: tumor necrosis factor receptor superfamily, member 25
Synonyms: WSL-1, Wsl, APO-3, TR3, DDR3, WSL-LR, LARD, TRAMP, DR3, Tnfrsf12
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 85030
Homologene: 13202
Tnfaip2
Name: tumor necrosis factor, alpha-induced protein 2
Synonyms: Tnfip2, Exoc3l3, M-sec
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 21928
Homologene: 4583
Adgrf4
Name: adhesion G protein-coupled receptor F4
Synonyms: 4632435A09Rik, Gpr115
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 78249
Homologene: 51953
Stab2
Name: stabilin 2
Synonyms: STAB-2, FEEL-2
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 192188
Homologene: 23022
Adgrv1
Name: adhesion G protein-coupled receptor V1
Synonyms: VLGR1, Mass1, Mgr1, Gpr98
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 110789
Homologene: 19815
Cdhr2
Name: cadherin-related family member 2
Synonyms: LOC268663, Pcdh24
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 268663
Homologene: 134510
Elapor2
Name: endosome-lysosome associated apoptosis and autophagy regulator family member 2
Synonyms: 9330182L06Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231014
Homologene: 27396
Ptprt
Name: protein tyrosine phosphatase receptor type T
Synonyms: RPTPrho
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 19281
HGNC: HGNC:9682
Homologene: 56924
Cerkl
Name: ceramide kinase-like
Synonyms: Rp26
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228094
Homologene: 52396
Acte1
Name: actin, epsilon 1
Synonyms: LOC244239, Gm498
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 102636989
Homologene: 131918
L3hypdh
Name: L-3-hydroxyproline dehydratase (trans-)
Synonyms: 2810055F11Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 67217
Homologene: 12100
Mylk
Name: myosin, light polypeptide kinase
Synonyms: telokin, Mlck, MLCK210, MLCK108, 9530072E15Rik, A930019C19Rik, nmMlck
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 107589
HGNC: HGNC:7590
Homologene: 14202
Pkp3
Name: plakophilin 3
Synonyms: 2310056L12Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 56460
HGNC: HGNC:9025
Homologene: 5200
Zbbx
Name: zinc finger, B-box domain containing
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 213234
Homologene: 11661
Slc34a1
Name: solute carrier family 34 (sodium phosphate), member 1
Synonyms: renal Na+/Pi transporter, Na/Pi cotransporter, Slc17a2, Npt2, NaPi-IIa
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 20505
VEGA: 13
Homologene: 20663
AC163020.1
Name:
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Hif3a
Name: hypoxia inducible factor 3, alpha subunit
Synonyms: MOP7, bHLHe17, Nepas
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 53417
Homologene: 9646
Prkcsh
Name: protein kinase C substrate 80K-H
Synonyms: 80K-H, hepatocystin
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 19089
VEGA: 9
HGNC: HGNC:9411
Homologene: 2056
Bmp8b
Name: bone morphogenetic protein 8b
Synonyms: Op3
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 12164
HGNC: HGNC:1075
Homologene: 130625
Slc22a2
Name: solute carrier family 22 (organic cation transporter), member 2
Synonyms: Oct2, Orct2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 20518
VEGA: 17
Homologene: 68293
Zhx3
Name: zinc fingers and homeoboxes 3
Synonyms: 4932418O04Rik, 1810059C13Rik, Tix1, 9530010N21Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 320799
Homologene: 9000
Or7g35
Name: olfactory receptor family 7 subfamily G member 35
Synonyms: GA_x6K02T2PVTD-13330461-13331399, MOR148-1, Olfr855
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 258517
HGNC: HGNC:8466
Homologene: 74176
Efhb
Name: EF hand domain family, member B
Synonyms: 4921525D22Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 211482
VEGA: 17
Homologene: 16982
Srsf8
Name: serine and arginine rich splicing factor 8
Synonyms: 4930595M18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 245492
Homologene: 138416
Ppp4r3c1
Name: protein phosphatase 4 regulatory subunit 3C1
Synonyms: 4930415L06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 245511
Homologene: 133109
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 51,799,530 bp
  • T to C, chromosome 1 at 66,415,740 bp
  • A to G, chromosome 2 at 35,283,983 bp
  • C to A, chromosome 2 at 79,428,794 bp
  • A to G, chromosome 2 at 152,250,575 bp
  • G to A, chromosome 2 at 160,780,889 bp
  • A to G, chromosome 2 at 161,566,117 bp
  • C to T, chromosome 3 at 75,105,671 bp
  • T to C, chromosome 4 at 52,462,897 bp
  • T to A, chromosome 4 at 123,121,878 bp
  • T to C, chromosome 4 at 152,119,601 bp
  • A to C, chromosome 5 at 9,440,877 bp
  • T to C, chromosome 6 at 125,033,237 bp
  • T to A, chromosome 7 at 17,044,919 bp
  • A to G, chromosome 7 at 39,537,306 bp
  • T to C, chromosome 7 at 47,310,211 bp
  • A to G, chromosome 7 at 141,088,737 bp
  • A to T, chromosome 7 at 143,897,303 bp
  • G to A, chromosome 8 at 13,291,959 bp
  • C to T, chromosome 8 at 90,956,557 bp
  • T to G, chromosome 9 at 19,584,714 bp
  • T to C, chromosome 9 at 22,010,615 bp
  • T to C, chromosome 9 at 24,643,781 bp
  • T to C, chromosome 9 at 119,608,968 bp
  • G to T, chromosome 10 at 86,858,124 bp
  • T to A, chromosome 11 at 70,917,367 bp
  • C to T, chromosome 11 at 78,154,929 bp
  • C to T, chromosome 11 at 102,860,110 bp
  • T to C, chromosome 12 at 72,085,116 bp
  • C to T, chromosome 12 at 111,451,344 bp
  • A to T, chromosome 13 at 54,718,266 bp
  • T to C, chromosome 13 at 55,402,661 bp
  • GA to GAA, chromosome 13 at 81,540,132 bp
  • A to G, chromosome 15 at 78,959,977 bp
  • C to A, chromosome 16 at 34,963,577 bp
  • A to T, chromosome 17 at 12,612,450 bp
  • A to T, chromosome 17 at 42,669,759 bp
  • A to G, chromosome 17 at 53,400,736 bp
  • A to T, chromosome 18 at 82,680,745 bp
  • A to G, chromosome X at 81,421,113 bp
  • T to C, chromosome X at 89,930,510 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4003 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040945-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.