Strain Name:
C57BL/6J-MtgxR4008Btlr/Mmmh
Stock Number:
040946-MU
Citation ID:
RRID:MMRRC_040946-MU
Other Names:
R4008 (G1), C57BL/6J-MtgxR4008Btlr
Major Collection:

Strain Information

Rgs14
Name: regulator of G-protein signaling 14
Synonyms: Rap1/rap2 interacting protein
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 51791
HGNC: HGNC:9996
Homologene: 4735
Adsl
Name: adenylosuccinate lyase
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 11564
HGNC: HGNC:291
Homologene: 12
Shroom3
Name: shroom family member 3
Synonyms: Shrm3, Shrm, D5Ertd287e
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 27428
Homologene: 9263
Pkn2
Name: protein kinase N2
Synonyms: Prkcl2, 6030436C20Rik, PRK2, Stk7
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 109333
HGNC: HGNC:9406
Homologene: 2054
Tshr
Name: thyroid stimulating hormone receptor
Synonyms: pet, hypothroid, hyt
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 22095
Homologene: 315
Oprm1
Name: opioid receptor, mu 1
Synonyms: Oprm, MOP receptor, muOR, mor, MOP-R, MOR-1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 18390
HGNC: HGNC:8156
Homologene: 37368
Rbm34
Name: RNA binding motif protein 34
Synonyms: 4930547K05Rik, D8Ertd233e
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 52202
Homologene: 56691
Pwp1
Name: PWP1 homolog, endonuclein
Synonyms: 2610205J09Rik, 2310058A11Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 103136
Homologene: 5129
St3gal3
Name: ST3 beta-galactoside alpha-2,3-sialyltransferase 3
Synonyms: ST3Gal III, Siat6, Siat3
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 20441
Homologene: 7539
Ripor2
Name: RHO family interacting cell polarization regulator 2
Synonyms: E430013J17Rik, Fam65b, 1700108N18Rik, 6330500D04Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 193385
Homologene: 9284
Larp7
Name: La ribonucleoprotein 7, transcriptional regulator
Synonyms: D3Wsu161e, C330027G06Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 28036
Homologene: 88843
Chd1l
Name: chromodomain helicase DNA binding protein 1-like
Synonyms: 4432404A22Rik, Snf2p
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 68058
HGNC: HGNC:1916
Homologene: 11590
Tars3
Name: threonyl-tRNA synthetase 3
Synonyms: A530046H20Rik, Tarsl2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 272396
Homologene: 65036
Smg5
Name: SMG5 nonsense mediated mRNA decay factor
Synonyms: Smg-5 homolog, nonsense mediated mRNA decay factor (C. elegans)
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229512
Homologene: 9095
Clvs2
Name: clavesin 2
Synonyms: Rlbp1l2, A330019N05Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 215890
Homologene: 27894
H2-K1
Name: histocompatibility 2, K1, K region
Synonyms: H-2K
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 14972
Homologene: 128352
Epha3
Name: Eph receptor A3
Synonyms: Tyro4, Hek, Hek4, Mek4, End3, Cek4
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 13837
VEGA: 16
HGNC: HGNC:3387
Homologene: 21083
Ifit2
Name: interferon-induced protein with tetratricopeptide repeats 2
Synonyms: Ifi54
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 15958
HGNC: HGNC:5409
Homologene: 1187
F830045P16Rik
Name: RIKEN cDNA F830045P16 gene
Synonyms: Sirpb3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228592
Homologene: 136180
Slc39a12
Name: solute carrier family 39 (zinc transporter), member 12
Synonyms: LOC277468
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 277468
Homologene: 17654
Col6a5
Name: collagen, type VI, alpha 5
Synonyms: Gm7455, Col6a5
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 665033
Homologene: 122792
Map9
Name: microtubule-associated protein 9
Synonyms: 5033421J10Rik, 5330427D05Rik, ASAP, Mtap9
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 213582
Homologene: 41591
Try10
Name: trypsin 10
Synonyms: trypsinogen 10
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 436522
Homologene: 134623
Or10ag57
Name: olfactory receptor family 10 subfamily AG member 57
Synonyms: Olfr1122, GA_x6K02T2Q125-48880078-48881058, MOR264-1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 259033
Homologene: 73977
Mn1
Name: meningioma 1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 433938
HGNC: HGNC:7180
Homologene: 37620
Npdc1
Name: neural proliferation, differentiation and control 1
Synonyms: NPDC-1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 18146
HGNC: HGNC:7899
Homologene: 32050
Rabep2
Name: rabaptin, RAB GTPase binding effector protein 2
Synonyms: 2610011A08Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 70314
Homologene: 23489
Acss2
Name: acyl-CoA synthetase short-chain family member 2
Synonyms: ACAS, AceCS1, Acas1, Acas2, acetyl-CoA synthetase 1, Acs1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 60525
Homologene: 6469
Serpina3a
Name: serine (or cysteine) peptidase inhibitor, clade A, member 3A
Synonyms: 4933406L18Rik, antitrypsin, alpha-1 antiproteinase,
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 74069
HGNC: HGNC:16
Or4n5
Name: olfactory receptor family 4 subfamily N member 5
Synonyms: Olfr722, MOR241-3, GA_x6K02T2PMLR-5566715-5565789
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 258487
Homologene: 17270
Phactr3
Name: phosphatase and actin regulator 3
Synonyms: 4930415A02Rik, scapinin, 1500003N10Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 74189
Homologene: 14482
Batf2
Name: basic leucine zipper transcription factor, ATF-like 2
Synonyms: 4933430F08Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 74481
Homologene: 12569
Mrps18c
Name: mitochondrial ribosomal protein S18C
Synonyms: 1110037D14Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 68735
Homologene: 9371
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 2 at 14,452,117 bp
  • T to A, chromosome 2 at 25,408,980 bp
  • A to T, chromosome 2 at 87,388,580 bp
  • A to T, chromosome 2 at 129,463,547 bp
  • T to A, chromosome 2 at 155,557,628 bp
  • T to C, chromosome 2 at 178,260,164 bp
  • T to C, chromosome 3 at 82,359,083 bp
  • T to C, chromosome 3 at 88,349,158 bp
  • A to T, chromosome 3 at 97,570,402 bp
  • T to C, chromosome 3 at 127,540,870 bp
  • T to C, chromosome 3 at 142,810,458 bp
  • T to C, chromosome 4 at 117,940,440 bp
  • G to T, chromosome 5 at 92,943,086 bp
  • T to C, chromosome 5 at 100,803,116 bp
  • G to T, chromosome 5 at 111,420,169 bp
  • A to G, chromosome 6 at 41,356,674 bp
  • T to A, chromosome 7 at 65,678,128 bp
  • C to A, chromosome 7 at 126,445,374 bp
  • T to C, chromosome 8 at 126,949,287 bp
  • A to G, chromosome 9 at 105,903,567 bp
  • A to G, chromosome 10 at 6,832,520 bp
  • G to A, chromosome 10 at 33,543,462 bp
  • A to G, chromosome 10 at 85,882,034 bp
  • C to T, chromosome 12 at 91,537,494 bp
  • A to T, chromosome 12 at 104,118,643 bp
  • A to G, chromosome 13 at 24,696,538 bp
  • T to G, chromosome 13 at 55,369,913 bp
  • A to G, chromosome 14 at 49,895,007 bp
  • T to C, chromosome 15 at 80,966,156 bp
  • G to A, chromosome 16 at 63,566,564 bp
  • G to A, chromosome 17 at 33,999,551 bp
  • G to A, chromosome 19 at 6,171,348 bp
  • G to T, chromosome 19 at 34,574,045 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4008 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040946-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.