Strain Name:
C57BL/6J-MtgxR4025Btlr/Mmmh
Stock Number:
040958-MU
Citation ID:
RRID:MMRRC_040958-MU
Other Names:
R4025 (G1), C57BL/6J-MtgxR4025Btlr
Major Collection:

Gene Information

Fezf2
Name: Fez family zinc finger 2
Synonyms: forebrain embryonic zinc finger, Fez, Fezl, Zfp312
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 54713
VEGA: 14
Homologene: 9957
Spns1
Name: spinster homolog 1
Synonyms: 2210013K02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 73658
Homologene: 41530
Myo1e
Name: myosin IE
Synonyms: 2310020N23Rik, 9130023P14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 71602
VEGA: 9
HGNC: HGNC:7599
Homologene: 55864
Spag7
Name: sperm associated antigen 7
Synonyms: FSA-1, Fsa1l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216873
Homologene: 3595
Clcn6
Name: chloride channel, voltage-sensitive 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 26372
HGNC: HGNC:2024
Homologene: 985
Wdfy3
Name: WD repeat and FYVE domain containing 3
Synonyms: Ggtb3, 2610509D04Rik, D5Ertd66e, Bwf1, Bchs, Alfy
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 72145
Homologene: 22855
Polr2a
Name: polymerase (RNA) II (DNA directed) polypeptide A
Synonyms: 220kDa, Rpo2-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 20020
HGNC: HGNC:9187
Homologene: 721
Kpnb1
Name: karyopherin (importin) beta 1
Synonyms: Impnb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 16211
HGNC: HGNC:6400
Homologene: 1707
Atad5
Name: ATPase family, AAA domain containing 5
Synonyms: LOC237877, C130052G03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237877
Homologene: 32611
Odf2
Name: outer dense fiber of sperm tails 2
Synonyms: MMTEST29, cenexin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 18286
HGNC: HGNC:8114
Homologene: 1907
Cdk5rap2
Name: CDK5 regulatory subunit associated protein 2
Synonyms: 2900018K03Rik, an
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 214444
Homologene: 49533
Ranbp2
Name: RAN binding protein 2
Synonyms: A430087B05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 19386
VEGA: 10
Homologene: 87808
Ate1
Name: arginyltransferase 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 11907
HGNC: HGNC:782
Homologene: 31420
Swi5
Name: SWI5 recombination repair homolog (yeast)
Synonyms: 1500019F05Rik, 2900010J23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 72931
Homologene: 128744
Tasor2
Name: transcription activation suppressor family member 2
Synonyms: BC016423, Fam208b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 105203
VEGA: 13
Homologene: 26435
Mctp2
Name: multiple C2 domains, transmembrane 2
Synonyms: LOC244049
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 244049
Homologene: 69254
Cacna1a
Name: calcium channel, voltage-dependent, P/Q type, alpha 1A subunit
Synonyms: Cacnl1a4, alpha1A, Ccha1a, SCA6, nmf352, smrl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 12286
HGNC: HGNC:1388
Homologene: 56383
Slfn4
Name: schlafen 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 20558
Homologene: 133236
Nutm2
Name: NUT family member 2
Synonyms: LOC328250, Gm806
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 328250
Homologene: 128615
Wdr11
Name: WD repeat domain 11
Synonyms: 2900055P10Rik, Wdr11, Brwd2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 207425
Homologene: 41229
Slc4a3
Name: solute carrier family 4 (anion exchanger), member 3
Synonyms: Ae3, A930038D23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 20536
Homologene: 129474
Ugcg
Name: UDP-glucose ceramide glucosyltransferase
Synonyms: GlcT-1, Epcs21, Ugcgl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 22234
Homologene: 37763
Xirp2
Name: xin actin-binding repeat containing 2
Synonyms: A530024P18Rik, 2310008C07Rik, 2310003D02Rik, mXin beta, myomaxin, Cmya3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 241431
Homologene: 19388
Stab1
Name: stabilin 1
Synonyms: MS-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 192187
Homologene: 9035
Stil
Name: Scl/Tal1 interrupting locus
Synonyms: Sil
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 20460
Homologene: 2283
Cap2
Name: CAP, adenylate cyclase-associated protein, 2 (yeast)
Synonyms: 2810452G09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 67252
Homologene: 101397
Papss2
Name: 3'-phosphoadenosine 5'-phosphosulfate synthase 2
Synonyms: Sk2, Atpsk2, 1810018P12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 23972
VEGA: 19
HGNC: HGNC:8604
Homologene: 55840
Top2b
Name: topoisomerase (DNA) II beta
Synonyms: Top-2, D230016L12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 21974
Homologene: 134711
Aoc1
Name: amine oxidase, copper-containing 1
Synonyms: 1600012D06Rik, Abp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 76507
HGNC: HGNC:80
Homologene: 68159
Zkscan6
Name: zinc finger with KRAB and SCAN domains 6
Synonyms: 1700128E15Rik, KOX11, D11Ertd714e, Zfp535
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 52712
Homologene: 12119
Ppm1d
Name: protein phosphatase 1D magnesium-dependent, delta isoform
Synonyms: Wip1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 53892
HGNC: HGNC:9277
Homologene: 31185
Grk3
Name: G protein-coupled receptor kinase 3
Synonyms: beta ARK2, Bark-2, Adrbk-2, 4833444A01Rik, Adrbk2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 320129
HGNC: HGNC:290
Homologene: 21072
Gm9920
Name: predicted gene 9920
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 100503794
Slc22a20
Name: solute carrier family 22 (organic anion transporter), member 20
Synonyms: LOC381203, mOAT6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 381203
Homologene: 87203
Ctnna2
Name: catenin (cadherin associated protein), alpha 2
Synonyms: alpha(N)-catenin, alpha N-catenin, Catna, chp, Catna2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 12386
HGNC: HGNC:2510
Homologene: 68394
Bivm
Name: basic, immunoglobulin-like variable motif containing
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 246229
Homologene: 9783
Fbxl7
Name: F-box and leucine-rich repeat protein 7
Synonyms: Fbl6, FBL7, D230018M15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 448987
VEGA: 15
Homologene: 69121
Vmn1r34
Name: vomeronasal 1 receptor 34
Synonyms: Gm5991
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 546901
Homologene: 110800
Or8d4
Name: olfactory receptor family 8 subfamily D member 4
Synonyms: GA_x6K02T2PVTD-33825763-33824828, mOR171-4, Olfr985
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258854
VEGA: 9
Homologene: 17416
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 44,137,128 bp
  • T to C, chromosome 1 at 75,549,041 bp
  • C to T, chromosome 2 at 29,926,815 bp
  • T to C, chromosome 2 at 32,281,788 bp
  • A to G, chromosome 2 at 67,511,402 bp
  • C to T, chromosome 4 at 59,207,798 bp
  • T to A, chromosome 4 at 70,250,387 bp
  • A to G, chromosome 4 at 115,009,258 bp
  • T to A, chromosome 4 at 148,014,283 bp
  • A to G, chromosome 5 at 101,924,095 bp
  • T to A, chromosome 5 at 112,914,984 bp
  • A to T, chromosome 6 at 48,908,269 bp
  • T to A, chromosome 6 at 66,637,704 bp
  • A to T, chromosome 6 at 77,636,844 bp
  • C to A, chromosome 7 at 72,090,239 bp
  • A to T, chromosome 7 at 126,376,946 bp
  • T to C, chromosome 7 at 129,625,148 bp
  • G to A, chromosome 7 at 130,504,681 bp
  • C to T, chromosome 8 at 84,581,333 bp
  • T to C, chromosome 9 at 40,127,500 bp
  • A to G, chromosome 9 at 70,324,875 bp
  • A to G, chromosome 10 at 58,480,556 bp
  • T to G, chromosome 11 at 65,822,817 bp
  • T to A, chromosome 11 at 69,743,659 bp
  • C to A, chromosome 11 at 70,664,474 bp
  • A to G, chromosome 11 at 80,120,686 bp
  • A to T, chromosome 11 at 83,187,214 bp
  • T to A, chromosome 11 at 85,345,757 bp
  • A to G, chromosome 11 at 97,169,893 bp
  • T to C, chromosome 13 at 3,584,554 bp
  • C to T, chromosome 13 at 46,637,841 bp
  • A to G, chromosome 13 at 50,469,353 bp
  • A to T, chromosome 14 at 12,343,986 bp
  • T to G, chromosome 14 at 16,409,189 bp
  • C to T, chromosome 14 at 31,154,952 bp
  • A to G, chromosome 15 at 26,552,819 bp
  • A to G, chromosome 15 at 55,112,570 bp
  • T to C, chromosome 19 at 5,985,780 bp
  • T to C, chromosome 19 at 32,651,923 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4025 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040958-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.