Strain Name:
C57BL/6J-MtgxR4031Btlr/Mmmh
Stock Number:
040960-MU
Citation ID:
RRID:MMRRC_040960-MU
Other Names:
R4031 (G1), C57BL/6J-MtgxR4031Btlr
Major Collection:

Gene Information

Ppp2r2a
Name: protein phosphatase 2, regulatory subunit B, alpha
Synonyms: 2410004D02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 71978
VEGA: 14
HGNC: HGNC:9304
Homologene: 2035
Neurod1
Name: neurogenic differentiation 1
Synonyms: BETA2, Neurod, Nd1, bHLHa3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 18012
HGNC: HGNC:7762
Homologene: 1871
Chrna5
Name: cholinergic receptor, nicotinic, alpha polypeptide 5
Synonyms: Acra5, Acra-5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 110835
VEGA: 9
HGNC: HGNC:1959
Homologene: 55485
Slc26a5
Name: solute carrier family 26, member 5
Synonyms: Pres, prestin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 80979
HGNC: HGNC:9359
Homologene: 69472
Ltbp3
Name: latent transforming growth factor beta binding protein 3
Synonyms: Ltbp2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 16998
VEGA: 19
HGNC: HGNC:6716
Homologene: 7405
Elf1
Name: E74-like factor 1
Synonyms: Elf-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 13709
VEGA: 14
HGNC: HGNC:3316
Homologene: 7303
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: trabeculin alpha, Acf7, Aclp7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 11426
Homologene: 136191
Mindy3
Name: MINDY lysine 48 deubiquitinase 3
Synonyms: 5830410F13Rik, 2310047O13Rik, Fam188a, 1810041E18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 66960
Homologene: 11478
Hspa12a
Name: heat shock protein 12A
Synonyms: 1700063D12Rik, Gm19925, Hspa12a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 73442
VEGA: 19
Homologene: 18422
Tm9sf4
Name: transmembrane 9 superfamily protein member 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 99237
Homologene: 21620
Wnk1
Name: WNK lysine deficient protein kinase 1
Synonyms: Hsn2, 6430573H23Rik, Prkwnk1, EG406236
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232341
Homologene: 14253
Copa
Name: coatomer protein complex subunit alpha
Synonyms: xenin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12847
HGNC: HGNC:2230
Homologene: 3218
Trak1
Name: trafficking protein, kinesin binding 1
Synonyms: hyrt, 2310001H13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 67095
Homologene: 25103
Polr2b
Name: polymerase (RNA) II (DNA directed) polypeptide B
Synonyms: RPB2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231329
HGNC: HGNC:9188
Homologene: 722
Zfp317
Name: zinc finger protein 317
Synonyms: Zfp67, D230022C05Rik, Zfp75, KRAB9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 244713
Homologene: 36389
Aplp2
Name: amyloid beta (A4) precursor-like protein 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 11804
VEGA: 9
HGNC: HGNC:598
Homologene: 20396
Psmd2
Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 2
Synonyms: Tex190, TEG-190, 9430095H01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 21762
HGNC: HGNC:9559
Homologene: 2101
Zfp236
Name: zinc finger protein 236
Synonyms: LOC240456
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 329002
Homologene: 7198
Rap1gds1
Name: RAP1, GTP-GDP dissociation stimulator 1
Synonyms: GDS1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229877
HGNC: HGNC:9859
Homologene: 41422
Zfp975
Name: zinc finger protein 975
Synonyms: Gm5595
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 434179
Homologene: 134004
Hyal4
Name: hyaluronoglucosaminidase 4
Synonyms: 4632428M18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 77042
HGNC: HGNC:5323
Homologene: 69117
Slurp1
Name: secreted Ly6/Plaur domain containing 1
Synonyms: ARS component B, 1110021N19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 57277
Homologene: 10710
Hydin
Name: HYDIN, axonemal central pair apparatus protein
Synonyms: hy3, hyrh, hy-3, 4930545D19Rik, 1700034M11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244653
Homologene: 52118
Ttn
Name: titin
Synonyms: shru, L56, mdm, 2310057K23Rik, 2310074I15Rik, 2310036G12Rik, 1100001C23Rik, connectin, D330041I19Rik, D830007G01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Kdm5d
Name: lysine (K)-specific demethylase 5D
Synonyms: HY, Jarid1d, Smcy
Type: Gene
Species: Mus musculus (mouse)
Chromosome: Y
NCBI: 20592
Homologene: 55838
Neb
Name: nebulin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 17996
HGNC: HGNC:7720
Homologene: 136285
Lrp1b
Name: low density lipoprotein-related protein 1B
Synonyms: 9630004P12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 94217
HGNC: HGNC:6693
Homologene: 56810
Abcc12
Name: ATP-binding cassette, sub-family C (CFTR/MRP), member 12
Synonyms: 4930467B22Rik, MRP9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244562
Homologene: 57211
Vwa3a
Name: von Willebrand factor A domain containing 3A
Synonyms: E030013G06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233813
Homologene: 18607
Avpi1
Name: arginine vasopressin-induced 1
Synonyms: mVIT32, 2310008N12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 69534
Homologene: 11027
Unc79
Name: unc-79 homolog
Synonyms: Mlca3, 9030205A07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 217843
Homologene: 41397
Tshz1
Name: teashirt zinc finger family member 1
Synonyms: teashirt1, D18Bwg1409e, Mtsh1, 5730407I04Rik, Sdccag33, Tsh1, NY-CO-33
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 110796
Homologene: 4227
Ccdc150
Name: coiled-coil domain containing 150
Synonyms: 4930511H11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 78016
Homologene: 15814
Kcnt1
Name: potassium channel, subfamily T, member 1
Synonyms: Slack, slo2, C030030G16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 227632
Homologene: 11055
Dgkz
Name: diacylglycerol kinase zeta
Synonyms: mDGK[z], E130307B02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 104418
HGNC: HGNC:2857
Homologene: 37831
Disp2
Name: dispatched RND tramsporter family member 2
Synonyms: B230210L08Rik, DispB
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 214240
Homologene: 24916
Vsig10l
Name: V-set and immunoglobulin domain containing 10 like
Synonyms: 2210412E05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 75690
Homologene: 35386
Crybb3
Name: crystallin, beta B3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 12962
HGNC: HGNC:2400
Homologene: 3008
Rsph10b
Name: radial spoke head 10 homolog B (Chlamydomonas)
Synonyms: 4930526H21Rik, Rsph10b2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Homologene: 18321
Polr1e
Name: polymerase (RNA) I polypeptide E
Synonyms: 53kDa, Praf1, Paf53, D030019D19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 64424
Homologene: 41486
Usp43
Name: ubiquitin specific peptidase 43
Synonyms: C630032K07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216835
Homologene: 129879
Nfasc
Name: neurofascin
Synonyms: D430023G06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 269116
Homologene: 24945
Vmn2r107
Name: vomeronasal 2, receptor 107
Synonyms: V2r6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 22312
Homologene: 129750
Fndc1
Name: fibronectin type III domain containing 1
Synonyms: 1110027O12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 68655
Klhl30
Name: kelch-like 30
Synonyms: 4631423F02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 70788
Homologene: 18891
C030005K15Rik
Name: RIKEN cDNA C030005K15 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Acss2
Name: acyl-CoA synthetase short-chain family member 2
Synonyms: AceCS1, ACAS, Acas1, Acs1, Acas2, acetyl-CoA synthetase 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 60525
Homologene: 6469
Dcun1d4
Name: DCN1, defective in cullin neddylation 1, domain containing 4 (S. cerevisiae)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100737
Homologene: 22867
Slc26a10
Name: solute carrier family 26, member 10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 216441
VEGA: 10
Homologene: 66953
Mblac2
Name: metallo-beta-lactamase domain containing 2
Synonyms: 2900024O10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 72852
VEGA: 13
Homologene: 15174
Spo11
Name: SPO11 meiotic protein covalently bound to DSB
Synonyms: Spo11b, Spo11a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 26972
Homologene: 6059
Arl2bp
Name: ADP-ribosylation factor-like 2 binding protein
Synonyms: 1700027H16Rik, 1700010P10Rik, Bart1, BART
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 107566
Homologene: 11479
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 54,278,811 bp
  • G to A, chromosome 1 at 91,361,157 bp
  • T to C, chromosome 1 at 132,582,984 bp
  • T to C, chromosome 1 at 172,108,375 bp
  • A to C, chromosome 2 at 12,401,083 bp
  • T to G, chromosome 2 at 25,916,048 bp
  • C to T, chromosome 2 at 40,702,848 bp
  • T to C, chromosome 2 at 52,281,449 bp
  • T to A, chromosome 2 at 76,754,824 bp
  • C to T, chromosome 2 at 79,454,026 bp
  • T to C, chromosome 2 at 91,939,309 bp
  • T to C, chromosome 2 at 118,791,880 bp
  • T to C, chromosome 2 at 153,198,344 bp
  • C to T, chromosome 2 at 155,557,210 bp
  • T to A, chromosome 2 at 172,986,832 bp
  • T to C, chromosome 3 at 139,050,592 bp
  • A to T, chromosome 4 at 45,018,685 bp
  • A to G, chromosome 4 at 123,381,312 bp
  • T to C, chromosome 5 at 21,847,191 bp
  • A to G, chromosome 5 at 73,534,637 bp
  • G to A, chromosome 5 at 77,348,405 bp
  • A to G, chromosome 5 at 113,079,869 bp
  • A to G, chromosome 5 at 143,985,668 bp
  • A to G, chromosome 6 at 24,756,224 bp
  • T to C, chromosome 6 at 119,951,068 bp
  • G to A, chromosome 7 at 42,662,953 bp
  • C to T, chromosome 7 at 43,465,374 bp
  • G to A, chromosome 7 at 120,768,232 bp
  • G to T, chromosome 8 at 86,517,448 bp
  • T to C, chromosome 8 at 94,667,653 bp
  • T to C, chromosome 8 at 110,610,047 bp
  • A to T, chromosome 9 at 19,646,712 bp
  • G to A, chromosome 9 at 31,157,730 bp
  • T to C, chromosome 9 at 54,998,086 bp
  • T to C, chromosome 9 at 121,451,670 bp
  • T to C, chromosome 10 at 97,725,542 bp
  • A to G, chromosome 10 at 127,178,002 bp
  • G to T, chromosome 11 at 67,913,833 bp
  • T to A, chromosome 12 at 103,072,759 bp
  • T to C, chromosome 13 at 81,750,089 bp
  • T to C, chromosome 14 at 67,028,976 bp
  • A to C, chromosome 14 at 79,569,283 bp
  • T to C, chromosome 15 at 74,727,487 bp
  • G to A, chromosome 16 at 20,663,205 bp
  • A to T, chromosome 17 at 7,769,752 bp
  • G to A, chromosome 17 at 20,375,221 bp
  • T to A, chromosome 18 at 82,624,465 bp
  • T to C, chromosome 18 at 84,014,829 bp
  • G to A, chromosome 19 at 5,754,022 bp
  • C to A, chromosome 19 at 42,124,741 bp
  • T to C, chromosome 19 at 58,800,857 bp
  • T to A, chromosome Y at 916,910 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4031 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040960-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.