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Strain Name:
C57BL/6J-MtgxR4031Btlr/Mmmh
Stock Number:
040960-MU
Citation ID:
RRID:MMRRC_040960-MU
Other Names:
R4031 (G1), C57BL/6J-MtgxR4031Btlr
Major Collection:

Strain Information

Ppp2r2a
Name: protein phosphatase 2, regulatory subunit B, alpha
Synonyms: 2410004D02Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 71978
VEGA: 14
HGNC: HGNC:9304
Homologene: 2035
Neurod1
Name: neurogenic differentiation 1
Synonyms: BETA2, Neurod, bHLHa3, Nd1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 18012
HGNC: HGNC:7762
Homologene: 1871
Chrna5
Name: cholinergic receptor, nicotinic, alpha polypeptide 5
Synonyms: Acra-5, Acra5
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 110835
VEGA: 9
HGNC: HGNC:1959
Homologene: 55485
Slc26a5
Name: solute carrier family 26, member 5
Synonyms: Pres, prestin
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 80979
HGNC: HGNC:9359
Homologene: 69472
Ltbp3
Name: latent transforming growth factor beta binding protein 3
Synonyms: Ltbp2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 16998
VEGA: 19
HGNC: HGNC:6716
Homologene: 7405
Elf1
Name: E74 like ETS transcription factor 1
Synonyms: Elf-1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 13709
VEGA: 14
HGNC: HGNC:3316
Homologene: 7303
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 54,278,811 bp
  • G to A, chromosome 1 at 91,361,157 bp
  • T to C, chromosome 1 at 132,582,984 bp
  • T to C, chromosome 1 at 172,108,375 bp
  • A to C, chromosome 2 at 12,401,083 bp
  • T to G, chromosome 2 at 25,916,048 bp
  • C to T, chromosome 2 at 40,702,848 bp
  • T to C, chromosome 2 at 52,281,449 bp
  • T to A, chromosome 2 at 76,754,824 bp
  • C to T, chromosome 2 at 79,454,026 bp
  • T to C, chromosome 2 at 91,939,309 bp
  • T to C, chromosome 2 at 118,791,880 bp
  • T to C, chromosome 2 at 153,198,344 bp
  • C to T, chromosome 2 at 155,557,210 bp
  • T to A, chromosome 2 at 172,986,832 bp
  • T to C, chromosome 3 at 139,050,592 bp
  • A to T, chromosome 4 at 45,018,685 bp
  • A to G, chromosome 4 at 123,381,312 bp
  • T to C, chromosome 5 at 21,847,191 bp
  • A to G, chromosome 5 at 73,534,637 bp
  • G to A, chromosome 5 at 77,348,405 bp
  • A to G, chromosome 5 at 113,079,869 bp
  • A to G, chromosome 5 at 143,985,668 bp
  • A to G, chromosome 6 at 24,756,224 bp
  • T to C, chromosome 6 at 119,951,068 bp
  • G to A, chromosome 7 at 42,662,953 bp
  • C to T, chromosome 7 at 43,465,374 bp
  • G to A, chromosome 7 at 120,768,232 bp
  • G to T, chromosome 8 at 86,517,448 bp
  • T to C, chromosome 8 at 94,667,653 bp
  • T to C, chromosome 8 at 110,610,047 bp
  • A to T, chromosome 9 at 19,646,712 bp
  • G to A, chromosome 9 at 31,157,730 bp
  • T to C, chromosome 9 at 54,998,086 bp
  • T to C, chromosome 9 at 121,451,670 bp
  • T to C, chromosome 10 at 97,725,542 bp
  • A to G, chromosome 10 at 127,178,002 bp
  • G to T, chromosome 11 at 67,913,833 bp
  • T to A, chromosome 12 at 103,072,759 bp
  • T to C, chromosome 13 at 81,750,089 bp
  • T to C, chromosome 14 at 67,028,976 bp
  • A to C, chromosome 14 at 79,569,283 bp
  • T to C, chromosome 15 at 74,727,487 bp
  • G to A, chromosome 16 at 20,663,205 bp
  • A to T, chromosome 17 at 7,769,752 bp
  • G to A, chromosome 17 at 20,375,221 bp
  • T to A, chromosome 18 at 82,624,465 bp
  • T to C, chromosome 18 at 84,014,829 bp
  • G to A, chromosome 19 at 5,754,022 bp
  • C to A, chromosome 19 at 42,124,741 bp
  • T to C, chromosome 19 at 58,800,857 bp
  • T to A, chromosome Y at 916,910 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4031 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040960-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.