Strain Name:
C57BL/6J-MtgxR4089Btlr/Mmmh
Stock Number:
040982-MU
Citation ID:
RRID:MMRRC_040982-MU
Other Names:
R4089 (G1), C57BL/6J-MtgxR4089Btlr
Major Collection:

Gene Information

Prkd3
Name: protein kinase D3
Synonyms: 5730497N19Rik, PKD3, 4930557O20Rik, Prkcn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 75292
HGNC: HGNC:9408
Homologene: 2055
Sox10
Name: SRY (sex determining region Y)-box 10
Synonyms: gt, Sox21
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 20665
VEGA: 15
Homologene: 5055
Mindy3
Name: MINDY lysine 48 deubiquitinase 3
Synonyms: 1810041E18Rik, Fam188a, 5830410F13Rik, 2310047O13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 66960
Homologene: 11478
Dock9
Name: dedicator of cytokinesis 9
Synonyms: Zizimin1, B230309H04Rik, D14Wsu89e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 105445
Homologene: 41026
Washc2
Name: WASH complex subunit 2`
Synonyms: Fam21, C530005J20Rik, D6Wsu116e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 28006
Homologene: 41686
Ddx4
Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
Synonyms: VASA, Mvh, mvh / m'vasa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 13206
VEGA: 13
Homologene: 49227
Ints4
Name: integrator complex subunit 4
Synonyms: 2610034N24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 101861
Homologene: 69427
Trip4
Name: thyroid hormone receptor interactor 4
Synonyms: 4930558E03Rik, ASC-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 56404
Homologene: 9426
Brca1
Name: breast cancer 1, early onset
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 12189
HGNC: HGNC:1100
Homologene: 5276
Cap1
Name: CAP, adenylate cyclase-associated protein 1 (yeast)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 12331
Homologene: 74572
Topbp1
Name: topoisomerase (DNA) II binding protein 1
Synonyms: D430026L04Rik, 1110031N14Rik, 2810429C13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 235559
Homologene: 38262
Gm14443
Name: predicted gene 14443
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
Sos1
Name: SOS Ras/Rac guanine nucleotide exchange factor 1
Synonyms: 4430401P03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 20662
VEGA: 17
Homologene: 4117
Lrig1
Name: leucine-rich repeats and immunoglobulin-like domains 1
Synonyms: Img, LIG-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 16206
Homologene: 7380
Arhgef17
Name: Rho guanine nucleotide exchange factor (GEF) 17
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 207212
Homologene: 129601
Gigyf2
Name: GRB10 interacting GYF protein 2
Synonyms: Tnrc15, A830080H02Rik, 2610016F01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 227331
Homologene: 41048
Il31ra
Name: interleukin 31 receptor A
Synonyms: GLM-R, GPL
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 218624
VEGA: 13
Homologene: 51395
Prmt9
Name: protein arginine methyltransferase 9
Synonyms: Prmt10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 102182
Homologene: 16298
Ehbp1
Name: EH domain binding protein 1
Synonyms: Flj21950, KIAA0903-like
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 216565
Homologene: 22880
Csmd1
Name: CUB and Sushi multiple domains 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 94109
Homologene: 69536
Marveld2
Name: MARVEL (membrane-associating) domain containing 2
Synonyms: Tric-c, Tric-a, Tricellulin, Tric-b, Tric, Mrvldc2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 218518
VEGA: 13
Homologene: 27037
Nek3
Name: NIMA (never in mitosis gene a)-related expressed kinase 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 23954
HGNC: HGNC:7746
Homologene: 1869
Lamc3
Name: laminin gamma 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 23928
HGNC: HGNC:6494
Homologene: 21222
Flt1
Name: FMS-like tyrosine kinase 1
Synonyms: vascular endothelial growth factor receptor-1, VEGFR-1, Flt-1, VEGFR1, sFlt1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 14254
HGNC: HGNC:3763
Homologene: 134179
Fbn2
Name: fibrillin 2
Synonyms: Sne, Fib-2, sy
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 14119
VEGA: 18
HGNC: HGNC:3604
Homologene: 1515
Ifngr1
Name: interferon gamma receptor 1
Synonyms: IFN-gamma R, IFN-gammaR, Ifngr, Ifgr, Nktar, CD119
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 15979
HGNC: HGNC:5439
Homologene: 359
Vmn2r115
Name: vomeronasal 2, receptor 115
Synonyms: V2Rp4, EG638102
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 638102
Homologene: 86604
Rnf135
Name: ring finger protein 135
Synonyms: 0610037N03Rik, MGC13061, U 2-3-0, 2410006N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 71956
Homologene: 12427
Acss3
Name: acyl-CoA synthetase short-chain family member 3
Synonyms: LOC380660, 8430416H19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 380660
Homologene: 11587
Mapk4
Name: mitogen-activated protein kinase 4
Synonyms: A330097D03Rik, Erk3-related, p63Mapk
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 225724
HGNC: HGNC:6878
Homologene: 2058
Frem3
Name: Fras1 related extracellular matrix protein 3
Synonyms: LOC333315
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 333315
Homologene: 35388
Trim65
Name: tripartite motif-containing 65
Synonyms: 4732463G12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 338364
Homologene: 27844
Tex14
Name: testis expressed gene 14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 83560
Homologene: 12838
Pkp3
Name: plakophilin 3
Synonyms: 2310056L12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 56460
HGNC: HGNC:9025
Homologene: 5200
Sypl2
Name: synaptophysin-like 2
Synonyms: Mg29
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 17306
Homologene: 7421
Gpr108
Name: G protein-coupled receptor 108
Synonyms: 1810015L19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 78308
VEGA: 17
Homologene: 32495
A530064D06Rik
Name: RIKEN cDNA A530064D06 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 328830
VEGA: 17
Homologene: 136361
Olfr1328
Name: olfactory receptor 1328
Synonyms: GA_x6K02T2QD9B-18602750-18603691, MOR259-1, MOR259-13, MOR259-1, Olfr1519
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 258394
Homologene: 121524
Rgs6
Name: regulator of G-protein signaling 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 50779
Homologene: 68385
Scn11a
Name: sodium channel, voltage-gated, type XI, alpha
Synonyms: NaT, NaN, SNS2, NSS2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 24046
VEGA: 9
Homologene: 8041
Cbs
Name: cystathionine beta-synthase
Synonyms: HIP4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 12411
Homologene: 37258
Dip2a
Name: disco interacting protein 2 homolog A
Synonyms: 4931420H10Rik, Dip2, Kiaa0184-hp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 64451
Homologene: 41012
Kcnk9
Name: potassium channel, subfamily K, member 9
Synonyms: Task3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 223604
VEGA: 15
HGNC: HGNC:6283
Homologene: 56758
Plau
Name: plasminogen activator, urokinase
Synonyms: u-PA, uPA, urokinase-type plasminogen activator
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 18792
VEGA: 14
HGNC: HGNC:9052
Homologene: 55670
Trim30d
Name: tripartite motif-containing 30D
Synonyms: TRIM30-3, Trim79
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 209387
Homologene: 114426
Actr5
Name: ARP5 actin-related protein 5
Synonyms: B430109J19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 109275
Homologene: 6818
1700012B07Rik
Name: RIKEN cDNA 1700012B07 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 69324
Homologene: 78000
Jpt2
Name: Jupiter microtubule associated homolog 2
Synonyms: 2810430B18Rik, D17Ertd441e, Hn1l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 52009
VEGA: 17
Homologene: 16934
Snap23
Name: synaptosomal-associated protein 23
Synonyms: Sndt, Syndet, SNAP-23
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 20619
Homologene: 37857
Gkn3
Name: gastrokine 3
Synonyms: 1190003M12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 68888
Homologene: 87422
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 87,443,672 bp
  • T to C, chromosome 2 at 12,364,516 bp
  • C to T, chromosome 2 at 31,920,508 bp
  • C to T, chromosome 2 at 120,584,375 bp
  • T to C, chromosome 2 at 158,625,102 bp
  • T to C, chromosome 2 at 175,171,892 bp
  • A to G, chromosome 3 at 108,217,676 bp
  • C to T, chromosome 4 at 118,934,033 bp
  • A to G, chromosome 4 at 122,862,409 bp
  • C to A, chromosome 5 at 147,564,241 bp
  • C to T, chromosome 6 at 87,383,525 bp
  • A to G, chromosome 6 at 94,609,859 bp
  • A to T, chromosome 6 at 116,256,292 bp
  • T to A, chromosome 7 at 97,529,255 bp
  • T to A, chromosome 7 at 100,883,799 bp
  • T to C, chromosome 7 at 104,487,800 bp
  • G to A, chromosome 7 at 141,084,143 bp
  • T to A, chromosome 8 at 15,992,738 bp
  • T to C, chromosome 8 at 22,149,913 bp
  • A to T, chromosome 8 at 77,572,545 bp
  • T to C, chromosome 8 at 80,615,173 bp
  • A to G, chromosome 9 at 65,858,283 bp
  • T to C, chromosome 9 at 103,324,501 bp
  • C to T, chromosome 9 at 119,795,653 bp
  • T to C, chromosome 10 at 19,601,485 bp
  • T to C, chromosome 10 at 76,278,489 bp
  • T to G, chromosome 10 at 107,053,452 bp
  • A to T, chromosome 11 at 22,095,898 bp
  • G to A, chromosome 11 at 80,199,046 bp
  • A to G, chromosome 11 at 87,512,203 bp
  • T to C, chromosome 11 at 101,524,176 bp
  • G to T, chromosome 11 at 109,794,154 bp
  • G to A, chromosome 11 at 116,126,479 bp
  • A to T, chromosome 12 at 83,063,487 bp
  • T to A, chromosome 13 at 100,600,480 bp
  • C to T, chromosome 13 at 112,551,919 bp
  • A to G, chromosome 13 at 112,613,761 bp
  • A to G, chromosome 14 at 20,841,066 bp
  • A to T, chromosome 14 at 121,583,471 bp
  • A to G, chromosome 15 at 72,546,263 bp
  • C to T, chromosome 15 at 79,156,363 bp
  • A to C, chromosome 17 at 23,346,384 bp
  • A to G, chromosome 17 at 24,956,102 bp
  • A to C, chromosome 17 at 31,633,006 bp
  • A to G, chromosome 17 at 48,166,510 bp
  • A to G, chromosome 17 at 57,237,925 bp
  • A to G, chromosome 17 at 78,971,388 bp
  • A to G, chromosome 17 at 80,449,352 bp
  • C to T, chromosome 18 at 58,053,769 bp
  • C to A, chromosome 18 at 73,930,459 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4089 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040982-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.