Strain Name:
C57BL/6J-MtgxR4114Btlr/Mmmh
Stock Number:
040990-MU
Citation ID:
RRID:MMRRC_040990-MU
Other Names:
R4114 (G1), C57BL/6J-MtgxR4114Btlr
Major Collection:

Strain Information

Morc3
Name: microrchidia 3
Synonyms: 1110051N18Rik, D16Jhu32e, 1110051N18Rik, Zcwcc3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 338467
Homologene: 32257
Nop56
Name: NOP56 ribonucleoprotein
Synonyms: NOP56, 56kDa with KKE/D repeat, 2310044F10Rik, Nol5a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 67134
Homologene: 4660
Zfp35
Name: zinc finger protein 35
Synonyms: Zfp-35
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 22694
Homologene: 133081
Ryr2
Name: ryanodine receptor 2, cardiac
Synonyms: 9330127I20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 20191
VEGA: 13
Homologene: 37423
Abcf1
Name: ATP-binding cassette, sub-family F (GCN20), member 1
Synonyms: Abc50, D17Wsu166e, GCN20
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224742
HGNC: HGNC:70
Homologene: 849
Hdc
Name: histidine decarboxylase
Synonyms: Hdc-s, Hdc-e, Hdc-c, Hdc-a, L-histidine decarboxylase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 15186
HGNC: HGNC:4855
Homologene: 20490
6030468B19Rik
Name: RIKEN cDNA 6030468B19 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 77727
Homologene: 90660
Sec22a
Name: SEC22 homolog A, vesicle trafficking protein
Synonyms: 1810005C06Rik, Sec22l2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 317717
Homologene: 8246
Tspan18
Name: tetraspanin 18
Synonyms: 2610042G18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 241556
Homologene: 24122
Abca16
Name: ATP-binding cassette, sub-family A (ABC1), member 16
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233810
Homologene: 132942
Cdh23
Name: cadherin 23 (otocadherin)
Synonyms: 4930542A03Rik, USH1D, mdfw, ahl, bob, nmf112, nmf181, nmf252, sals
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 22295
Homologene: 11142
Tek
Name: TEK receptor tyrosine kinase
Synonyms: Hyk, Tie2, Cd202b, tie-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 21687
Homologene: 397
Nlrp4a
Name: NLR family, pyrin domain containing 4A
Synonyms: Nalp-eta, E330028A19Rik, Nalp4a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 243880
Homologene: 79696
Itpr2
Name: inositol 1,4,5-triphosphate receptor 2
Synonyms: Ip3r2, Itpr5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 16439
HGNC: HGNC:6181
Homologene: 37593
Slc7a1
Name: solute carrier family 7 (cationic amino acid transporter, y+ system), member 1
Synonyms: Atrc-1, Rev-1, Rec-1, Atrc1, 4831426K01Rik, mCAT-1, Cat1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 11987
Homologene: 20658
Scart2
Name: scavenger receptor family member expressed on T cells 2
Synonyms: 5830411N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 244234
Homologene: 133218
Ankrd34c
Name: ankyrin repeat domain 34C
Synonyms: LOC330998, B230218L05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 330998
Homologene: 19814
Gbe1
Name: glucan (1,4-alpha-), branching enzyme 1
Synonyms: 2310045H19Rik, 2810426P10Rik, D16Ertd536e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 74185
HGNC: HGNC:4180
Homologene: 129
Dpp6
Name: dipeptidylpeptidase 6
Synonyms: Dpp-6, Peplb, In(5)6H-p, Rw, LOC384168, B930011P16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 13483
HGNC: HGNC:3010
Homologene: 22560
Pde8a
Name: phosphodiesterase 8A
Synonyms: Pde8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 18584
HGNC: HGNC:8793
Homologene: 1957
Or6c6c
Name: olfactory receptor family 6 subfamily C member 6C
Synonyms: GA_x6K02T2PULF-11383575-11384519, MOR110-7, Olfr804
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 258068
Homologene: 133581
Vmn2r37
Name: vomeronasal 2, receptor 37
Synonyms: V2r14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 22305
Homologene: 113703
Zbbx
Name: zinc finger, B-box domain containing
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 213234
Homologene: 11661
Or8u9
Name: olfactory receptor family 8 subfamily U member 9
Synonyms: GA_x6K02T2Q125-47640742-47639798, MOR185-4, Olfr1044
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 259013
Homologene: 100542
Trim5
Name: tripartite motif-containing 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 667823
Homologene: 75345
Colq
Name: collagen-like tail subunit (single strand of homotrimer) of asymmetric acetylcholinesterase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 382864
HGNC: HGNC:2226
Ino80b
Name: INO80 complex subunit B
Synonyms: Papa1, HMG1YL4, 2510009I23Rik, Hmga1l4, Znhit4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 70020
Homologene: 32566
Mpped1
Name: metallophosphoesterase domain containing 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 223726
HGNC: HGNC:1306
Homologene: 15012
Cep44
Name: centrosomal protein 44
Synonyms: 4933440G23Rik, BC088983
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 382010
Homologene: 12738
Pcdhb3
Name: protocadherin beta 3
Synonyms: PcdhbC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 93874
HGNC: HGNC:8688
Homologene: 115665
Nek5
Name: NIMA (never in mitosis gene a)-related expressed kinase 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 330721
HGNC: HGNC:7748
Homologene: 87952
Sult2a2
Name: sulfotransferase family 2A, dehydroepiandrosterone (DHEA)-preferring, member 2
Synonyms: mSTa2, Sth2, C730007P19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 100043194
Cym
Name: chymosin
Synonyms: LOC229697, Gm131
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229697
Homologene: 129841
Gm12359
Name: predicted gene 12359
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 100037262
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 2 at 86,171,415 bp
  • C to A, chromosome 2 at 93,311,946 bp
  • T to A, chromosome 2 at 126,601,818 bp
  • T to C, chromosome 2 at 130,276,673 bp
  • T to C, chromosome 3 at 75,139,598 bp
  • A to T, chromosome 3 at 107,219,749 bp
  • T to C, chromosome 4 at 94,849,683 bp
  • A to T, chromosome 4 at 144,209,603 bp
  • T to A, chromosome 5 at 27,469,487 bp
  • T to C, chromosome 5 at 148,342,057 bp
  • A to G, chromosome 6 at 83,124,140 bp
  • C to T, chromosome 6 at 146,425,510 bp
  • T to A, chromosome 7 at 9,210,093 bp
  • A to T, chromosome 7 at 13,734,783 bp
  • T to G, chromosome 7 at 26,449,940 bp
  • T to A, chromosome 7 at 81,282,807 bp
  • A to G, chromosome 7 at 104,265,740 bp
  • T to C, chromosome 7 at 120,527,067 bp
  • T to C, chromosome 7 at 140,297,910 bp
  • G to A, chromosome 8 at 22,111,162 bp
  • T to C, chromosome 8 at 56,545,422 bp
  • A to G, chromosome 9 at 89,729,874 bp
  • A to G, chromosome 10 at 60,421,040 bp
  • T to C, chromosome 10 at 129,705,799 bp
  • T to C, chromosome 11 at 98,810,010 bp
  • T to G, chromosome 11 at 117,802,967 bp
  • C to T, chromosome 13 at 11,692,682 bp
  • T to A, chromosome 14 at 31,557,867 bp
  • A to G, chromosome 15 at 83,796,709 bp
  • A to G, chromosome 16 at 35,318,832 bp
  • G to T, chromosome 16 at 70,483,827 bp
  • G to A, chromosome 16 at 93,873,339 bp
  • A to T, chromosome 17 at 35,959,254 bp
  • A to C, chromosome 18 at 24,002,709 bp
  • A to G, chromosome 18 at 37,302,040 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4114 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040990-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.