Strain Name:
C57BL/6J-MtgxR4135Btlr/Mmmh
Stock Number:
040995-MU
Citation ID:
RRID:MMRRC_040995-MU
Other Names:
R4135 (G1), C57BL/6J-MtgxR4135Btlr
Major Collection:

Strain Information

Cdc25a
Name: cell division cycle 25A
Synonyms: D9Ertd393e
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 12530
HGNC: HGNC:1725
Homologene: 1355
Sema6d
Name: sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D
Synonyms: 1110067B02Rik, Sema6D-2, Sema6D-4, Sema6D-5, Sema6D-1, Sema6D-6
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 214968
Homologene: 16195
Mthfd1
Name: methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthase
Synonyms: Mthfd, E430024A07Rik, DCS
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 108156
VEGA: 12
HGNC: HGNC:7432
Homologene: 55940
Chst5
Name: carbohydrate sulfotransferase 5
Synonyms: I-GlcNAc6ST, GST-4
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 56773
Homologene: 56927
Zfp729a
Name: zinc finger protein 729a
Synonyms: A530054K11Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 212281
Homologene: 133713
Cux1
Name: cut-like homeobox 1
Synonyms: Cux-1, Cux, CDP, Cutl1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 13047
HGNC: HGNC:2557
Gsk3b
Name: glycogen synthase kinase 3 beta
Synonyms: GSK-3, GSK3, GSK-3beta, 7330414F15Rik, 8430431H08Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 56637
HGNC: HGNC:4617
Homologene: 55629
Rapsn
Name: receptor-associated protein of the synapse
Synonyms: Raps, 43kDa acetylcholine receptor-associated protein, Nraps, rapsyn
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 19400
HGNC: HGNC:9863
Homologene: 3708
Washc3
Name: WASH complex subunit 3
Synonyms: 5730495F03Rik, 2900091E11Rik, Ccdc53
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 67282
Homologene: 9364
Rreb1
Name: ras responsive element binding protein 1
Synonyms: 1110037N09Rik, B930013M22Rik, sao
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 68750
Homologene: 2218
Cbx3
Name: chromobox 3
Synonyms: heterochromatin protein 1 gamma, HP1g, M32
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 12417
HGNC: HGNC:1553
Homologene: 40583
Hbp1
Name: high mobility group box transcription factor 1
Synonyms: C86454, 1700058O05Rik, C330012F01Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 73389
Homologene: 8171
Gcnt2
Name: glucosaminyl (N-acetyl) transferase 2 (I blood group)
Synonyms: IGnTA, 5330430K10Rik, IGnT, IGnTC, IGnTB
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 14538
VEGA: 13
HGNC: HGNC:4204
Homologene: 41535
Pold3
Name: polymerase (DNA-directed), delta 3, accessory subunit
Synonyms: 2410142G14Rik, C85233, P68, P66, GC12
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 67967
Homologene: 38202
Csnk1g2
Name: casein kinase 1, gamma 2
Synonyms: 2810429I12Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 103236
VEGA: 10
HGNC: HGNC:2455
Homologene: 100845
Htr5a
Name: 5-hydroxytryptamine (serotonin) receptor 5A
Synonyms: Htr5
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 15563
HGNC: HGNC:5300
Homologene: 22461
Dock2
Name: dedicator of cyto-kinesis 2
Synonyms: MBC, CED-5, Hch
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 94176
HGNC: HGNC:2988
Homologene: 37984
Rorc
Name: RAR-related orphan receptor gamma
Synonyms: Thor, RORgamma, thymus orphan receptor
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 19885
Homologene: 21051
Nkx6-1
Name: NK6 homeobox 1
Synonyms: NKX6A, Nkx6.1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 18096
HGNC: HGNC:7839
Homologene: 4495
Rusc2
Name: RUN and SH3 domain containing 2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 100213
Homologene: 18967
Mcc
Name: mutated in colorectal cancers
Synonyms: D18Ertd451e
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 328949
HGNC: HGNC:6935
Homologene: 20539
Xirp2
Name: xin actin-binding repeat containing 2
Synonyms: 2310008C07Rik, myomaxin, mXin beta, A530024P18Rik, 2310003D02Rik, Cmya3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 241431
Homologene: 19388
Zfp352
Name: zinc finger protein 352
Synonyms: 2czf48
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 236537
Homologene: 45160
Vwa5b1
Name: von Willebrand factor A domain containing 5B1
Synonyms: 4931403E03Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 75718
Homologene: 19431
Phtf1
Name: putative homeodomain transcription factor 1
Synonyms: Phft
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18685
HGNC: HGNC:8939
Homologene: 4817
Dennd2d
Name: DENN domain containing 2D
Synonyms: 2010308M01Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 72121
Homologene: 11776
Zfp1004
Name: zinc finger protein 1004
Synonyms: Gm14139
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 100271882
Homologene: 134546
Hsd11b2
Name: hydroxysteroid 11-beta dehydrogenase 2
Synonyms: 11(beta)-HSD2, 11HSD2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 15484
HGNC: HGNC:5209
Homologene: 20088
Nlrp5
Name: NLR family, pyrin domain containing 5
Synonyms: Mater, Nalp5, Op1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 23968
Homologene: 65105
Gabrb3
Name: GABRB3, gamma-aminobutyric acid type A receptor subunit beta 3
Synonyms: Gabrb-3, beta3, A230092K12Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 14402
HGNC: HGNC:4083
Homologene: 633
Or5af1
Name: olfactory receptor family 5 subfamily AF member 1
Synonyms: GA_x6K02T2NKPP-575829-574903, MOR222-4P, Olfr312
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 258065
Homologene: 77375
Or2ag13
Name: olfactory receptor family 2 subfamily AG member 13
Synonyms: MOR283-6, Olfr695, GA_x6K02T2PBJ9-9092181-9091234
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258591
Homologene: 133597
Or2l13b
Name: olfactory receptor family 2 subfamily L member 13B
Synonyms: Olfr168, GA_x54KRFPKG5P-15979009-15978071, MOR271-1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 258354
Homologene: 128057
Ugt3a1
Name: UDP glycosyltransferases 3 family, polypeptide A1
Synonyms: Ugt3a2
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223337
Homologene: 122787
Or2y3
Name: olfactory receptor family 2 subfamily Y member 3
Synonyms: MOR256-4, Olfr131, GA_x6K02T2PSCP-2531299-2530355
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 258867
Homologene: 105889
9230110F15Rik
Name: RIKEN cDNA 9230110F15 gene
Synonyms:
Type: Gene
Species: Mouse
Chromosome: 9
Bak1
Name: BCL2-antagonist/killer 1
Synonyms: Bak, N-Bak
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 12018
HGNC: HGNC:949
Homologene: 917
Zfp810
Name: zinc finger protein 810
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235050
VEGA: 9
Homologene: 138299
Mrpl32
Name: mitochondrial ribosomal protein L32
Synonyms: 0610033O15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 75398
VEGA: 13
Homologene: 32711
Dcaf12l1
Name: DDB1 and CUL4 associated factor 12-like 1
Synonyms: Wdr40b, A230038L21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 245404
Homologene: 135953
Msl1
Name: male specific lethal 1
Synonyms: 4930463F05Rik, 4121402D02Rik, 2810017F12Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 74026
Homologene: 129785
4930523C07Rik
Name: RIKEN cDNA 4930523C07 gene
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 67647
Homologene: 124386
Or4l1
Name: olfactory receptor family 4 subfamily L member 1
Synonyms: Olfr723, GA_x6K02T2PMLR-5600424-5599495, MOR247-4, MOR247-3P
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 259147
Homologene: 71986
Ctsr
Name: cathepsin R
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 56835
VEGA: 13
Homologene: 75127
Vmn2r129
Name: vomeronasal 2, receptor 129
Synonyms: V2r9, V2r8, Vmn2r-ps160, Vmn2r-ps159
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 22314
Gm3962
Name: predicted gene 3962
Synonyms: Gm4033
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 100042681
G730046D07Rik
Name: RIKEN cDNA G730046D07 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Rpl26-ps2
Name: ribosomal protein L26, pseudogene 2
Synonyms: Gm4805
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 217600
VEGA: 12
Gm6987
Name: predicted pseudogene 6987
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 629591
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 160,077,522 bp
  • A to G, chromosome 2 at 67,525,397 bp
  • A to T, chromosome 2 at 91,036,817 bp
  • T to C, chromosome 2 at 124,664,120 bp
  • A to T, chromosome 2 at 150,181,868 bp
  • C to A, chromosome 3 at 94,389,519 bp
  • T to A, chromosome 3 at 103,996,697 bp
  • T to A, chromosome 3 at 106,482,661 bp
  • G to A, chromosome 4 at 43,425,563 bp
  • C to T, chromosome 4 at 90,225,024 bp
  • A to T, chromosome 4 at 138,594,330 bp
  • T to G, chromosome 4 at 156,338,790 bp
  • A to G, chromosome 5 at 27,842,692 bp
  • T to A, chromosome 5 at 101,659,505 bp
  • T to C, chromosome 5 at 136,307,896 bp
  • A to T, chromosome 6 at 51,481,795 bp
  • T to C, chromosome 7 at 8,739,724 bp
  • T to C, chromosome 7 at 23,418,398 bp
  • G to T, chromosome 7 at 57,591,288 bp
  • T to A, chromosome 7 at 100,100,647 bp
  • A to G, chromosome 7 at 106,714,003 bp
  • C to G, chromosome 7 at 126,566,299 bp
  • T to C, chromosome 8 at 105,523,166 bp
  • T to C, chromosome 8 at 111,890,184 bp
  • T to C, chromosome 9 at 22,279,073 bp
  • A to G, chromosome 9 at 35,839,428 bp
  • C to A, chromosome 9 at 109,881,517 bp
  • T to C, chromosome 10 at 80,638,296 bp
  • G to A, chromosome 10 at 88,219,280 bp
  • T to C, chromosome 11 at 34,714,501 bp
  • C to A, chromosome 11 at 58,831,994 bp
  • A to G, chromosome 11 at 98,796,300 bp
  • A to T, chromosome 12 at 31,934,422 bp
  • T to A, chromosome 12 at 57,632,795 bp
  • G to A, chromosome 12 at 76,282,874 bp
  • C to T, chromosome 13 at 14,612,979 bp
  • A to T, chromosome 13 at 37,947,123 bp
  • T to A, chromosome 13 at 40,887,807 bp
  • T to C, chromosome 13 at 61,161,270 bp
  • C to A, chromosome 13 at 67,619,806 bp
  • T to C, chromosome 14 at 49,928,815 bp
  • A to T, chromosome 15 at 9,338,724 bp
  • T to A, chromosome 16 at 19,530,702 bp
  • G to T, chromosome 16 at 38,191,624 bp
  • T to A, chromosome 17 at 27,021,270 bp
  • A to G, chromosome 17 at 38,082,357 bp
  • T to C, chromosome 18 at 44,724,640 bp
  • T to C, chromosome X at 44,789,453 bp
  • T to A, chromosome X at 94,024,610 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4135 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040995-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.