Strain Name:
C57BL/6J-MtgxR4162Btlr/Mmmh
Stock Number:
041005-MU
Citation ID:
RRID:MMRRC_041005-MU
Other Names:
R4162 (G1), C57BL/6J-MtgxR4162Btlr
Major Collection:

Gene Information

Myo10
Name: myosin X
Synonyms: myosin-X, D15Ertd600e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 17909
HGNC: HGNC:7593
Homologene: 36328
Atrn
Name: attractin
Synonyms: Mgca
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 11990
HGNC: HGNC:885
Homologene: 22542
Prkcd
Name: protein kinase C, delta
Synonyms: PKC[d], D14Ertd420e, PKCdelta, Pkcd
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 18753
HGNC: HGNC:9399
Homologene: 55963
Nrxn2
Name: neurexin II
Synonyms: 6430591O13Rik, neurexin II beta, neurexin II alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 18190
HGNC: HGNC:8009
Homologene: 86984
Mfn1
Name: mitofusin 1
Synonyms: 6330416C07Rik, 2310002F04Rik, D3Ertd265e, HR2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 67414
Homologene: 11481
Kdm5b
Name: lysine (K)-specific demethylase 5B
Synonyms: Rb-Bp2, Plu1, 2210016I17Rik, Jarid1b, D1Ertd202e, PLU-1, 2010009J12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 75605
Homologene: 48448
Itsn1
Name: intersectin 1 (SH3 domain protein 1A)
Synonyms: Eh domain, SH3 domain regulator of endocytosis 1, EHSH1, Sh3p17, Ese1, Intersectin-L
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 16443
HGNC: HGNC:6183
Homologene: 2277
Prep
Name: prolyl endopeptidase
Synonyms: D10Wsu136e, Pop, prolyl oligopeptidase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 19072
VEGA: 10
HGNC: HGNC:9358
Homologene: 2042
Carmil1
Name: capping protein regulator and myosin 1 linker 1
Synonyms: Lrrc16a, Carmil, 1110037D04Rik, Lrrc16
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 68732
Homologene: 9757
Vasp
Name: vasodilator-stimulated phosphoprotein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 22323
Homologene: 7592
Zfhx3
Name: zinc finger homeobox 3
Synonyms: Atbf1, WBP9, Sci, A230102L03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 11906
HGNC: HGNC:777
Homologene: 21366
Flnb
Name: filamin, beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 286940
HGNC: HGNC:3755
Homologene: 37480
Cep57
Name: centrosomal protein 57
Synonyms: Tsp57, 4931428M20Rik, 3110002L15Rik, 4921510P06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 74360
Homologene: 8790
Tubgcp3
Name: tubulin, gamma complex associated protein 3
Synonyms: GCP3, Spc98p
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 259279
Homologene: 4609
Mef2b
Name: myocyte enhancer factor 2B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 17259
HGNC: HGNC:6995
Homologene: 48381
Tex15
Name: testis expressed gene 15
Synonyms: 2210014E14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 104271
Homologene: 12837
Ush2a
Name: usherin
Synonyms: LOC381317, A930011D15Rik, LOC269160, MUSH2A, A930037M10Rik, Ushrn, Ush2a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 22283
Homologene: 66151
Dnah3
Name: dynein, axonemal, heavy chain 3
Synonyms: Dnahc3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 381917
HGNC: HGNC:2949
Homologene: 19674
Dpysl4
Name: dihydropyrimidinase-like 4
Synonyms: Ulip4, unc-33-like phosphoprotein 4, Crmp3, DPY4, Drp-4, CRMP-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 26757
HGNC: HGNC:3016
Homologene: 4691
Plcb2
Name: phospholipase C, beta 2
Synonyms: B230205M18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 18796
HGNC: HGNC:9055
Homologene: 20957
Defb41
Name: defensin beta 41
Synonyms: Gm15386, 9230102D03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 77673
Nsun4
Name: NOL1/NOP2/Sun domain family, member 4
Synonyms: 2810405F18Rik, 2310010O12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 72181
Homologene: 12455
Thap12
Name: THAP domain containing 12
Synonyms: 2900052B10Rik, Prkrir, Dap4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 72981
HGNC: HGNC:9440
Homologene: 37952
Il18
Name: interleukin 18
Synonyms: Igif, Il-18
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 16173
VEGA: 9
HGNC: HGNC:5986
Homologene: 1200
Gli3
Name: GLI-Kruppel family member GLI3
Synonyms: brachyphalangy, Bph
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 14634
HGNC: HGNC:4319
Homologene: 139
Plch2
Name: phospholipase C, eta 2
Synonyms: Plcl4, A930027K05Rik, PLCeta2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 269615
Homologene: 85172
Gm10722
Name: predicted gene 10722
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Aldh1l2
Name: aldehyde dehydrogenase 1 family, member L2
Synonyms: D330038I09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 216188
Homologene: 51942
Cgas
Name: cyclic GMP-AMP synthase
Synonyms: Mb21d1, E330016A19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 214763
Homologene: 51384
Spout1
Name: SPOUT domain containing methyltransferase 1
Synonyms: D2Wsu81e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 227695
Homologene: 6704
Cybc1
Name: cytochrome b 245 chaperone 1
Synonyms: BC017643
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217370
Homologene: 51645
Grin2d
Name: glutamate receptor, ionotropic, NMDA2D (epsilon 4)
Synonyms: GluN2D, NR2D, NMDAR2D, GluRepsilon4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 14814
HGNC: HGNC:4588
Homologene: 648
Ifi203-ps
Name: interferon activated gene 203, pseudogene
Synonyms: Gm16340
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 100504287
HGNC: HGNC:5395
Homologene: 115929
Snx21
Name: sorting nexin family member 21
Synonyms: 5730407K14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 101113
Homologene: 43132
Ttc39c
Name: tetratricopeptide repeat domain 39C
Synonyms: 2810439F02Rik, 1700008N02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 72747
Homologene: 124438
Tex45
Name: testis expressed 45
Synonyms: 1700019B03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 76406
Homologene: 52367
Gm6866
Name: predicted gene 6866
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 628304
Homologene: 80005
4930404N11Rik
Name: RIKEN cDNA 4930404N11 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 432479
Homologene: 47801
Trav3-1
Name: T cell receptor alpha variable 3-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 667441
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 18,260,597 bp
  • C to T, chromosome 1 at 134,625,161 bp
  • A to G, chromosome 1 at 173,776,556 bp
  • A to C, chromosome 1 at 188,743,680 bp
  • C to T, chromosome 2 at 30,177,577 bp
  • T to A, chromosome 2 at 118,709,587 bp
  • A to G, chromosome 2 at 130,994,228 bp
  • T to C, chromosome 2 at 164,786,850 bp
  • T to C, chromosome 3 at 32,562,998 bp
  • G to T, chromosome 4 at 116,034,194 bp
  • T to C, chromosome 4 at 154,979,594 bp
  • A to T, chromosome 7 at 19,259,472 bp
  • A to G, chromosome 7 at 41,217,778 bp
  • T to C, chromosome 7 at 45,857,618 bp
  • C to T, chromosome 7 at 98,710,078 bp
  • C to T, chromosome 7 at 119,922,838 bp
  • T to C, chromosome 7 at 139,098,574 bp
  • C to T, chromosome 8 at 3,479,067 bp
  • C to T, chromosome 8 at 12,639,547 bp
  • T to C, chromosome 8 at 33,581,558 bp
  • T to C, chromosome 8 at 70,166,311 bp
  • C to A, chromosome 8 at 108,956,987 bp
  • A to C, chromosome 9 at 3,001,041 bp
  • C to T, chromosome 9 at 13,812,633 bp
  • T to C, chromosome 9 at 50,579,412 bp
  • G to A, chromosome 9 at 78,434,404 bp
  • G to T, chromosome 10 at 45,067,362 bp
  • A to G, chromosome 10 at 81,361,097 bp
  • T to A, chromosome 10 at 83,506,654 bp
  • T to C, chromosome 11 at 121,224,666 bp
  • G to T, chromosome 13 at 15,725,115 bp
  • G to A, chromosome 13 at 24,074,045 bp
  • A to T, chromosome 14 at 7,915,374 bp
  • A to G, chromosome 14 at 30,601,197 bp
  • T to C, chromosome 14 at 52,581,039 bp
  • T to A, chromosome 15 at 25,726,415 bp
  • C to A, chromosome 16 at 91,852,902 bp
  • A to G, chromosome 18 at 12,724,937 bp
  • G to A, chromosome 19 at 6,532,143 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4162 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041005-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.