Strain Name:
C57BL/6J-MtgxR4165Btlr/Mmmh
Stock Number:
041007-MU
Citation ID:
RRID:MMRRC_041007-MU
Other Names:
R4165 (G1), C57BL/6J-MtgxR4165Btlr
Major Collection:

Gene Information

Espl1
Name: extra spindle pole bodies 1, separase
Synonyms: ESP1, Cerp, PRCE, PRCE, SSE, separase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 105988
VEGA: 15
Homologene: 32151
Ankfy1
Name: ankyrin repeat and FYVE domain containing 1
Synonyms: Ankhzn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 11736
Homologene: 9491
Epb41l3
Name: erythrocyte membrane protein band 4.1 like 3
Synonyms: Epb4.1l3, DAL1P, NBL3, 4.1B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 13823
HGNC: HGNC:3380
Homologene: 49308
Snapc1
Name: small nuclear RNA activating complex, polypeptide 1
Synonyms: 9630050P21Rik, 2700033G17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 75627
VEGA: 12
Homologene: 2317
Tomm22
Name: translocase of outer mitochondrial membrane 22
Synonyms: Tom22
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 223696
Homologene: 10638
Ralgapa1
Name: Ral GTPase activating protein, alpha subunit 1
Synonyms: Tulip1, 2310003F20Rik, 4930400K19Rik, Garnl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 56784
VEGA: 12
Homologene: 84805
Kdm3b
Name: KDM3B lysine (K)-specific demethylase 3B
Synonyms: JHDM2B, Jmjd1b, 5830462I21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 277250
VEGA: 18
HGNC: HGNC:1337
Homologene: 41145
Pigr
Name: polymeric immunoglobulin receptor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 18703
HGNC: HGNC:8968
Homologene: 1984
Larp7
Name: La ribonucleoprotein domain family, member 7
Synonyms: C330027G06Rik, D3Wsu161e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 28036
Homologene: 88843
Aak1
Name: AP2 associated kinase 1
Synonyms: D6Ertd245e, 5530400K14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 269774
Homologene: 128746
Rps3
Name: ribosomal protein S3
Synonyms: D7Ertd795e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 27050
Homologene: 779
Txnl4a
Name: thioredoxin-like 4A
Synonyms: Txnl4, U5-15kDa, ENSMUSG00000057130, D18Wsu98e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 27366
Homologene: 7150
Prap1
Name: proline-rich acidic protein 1
Synonyms: Upa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 22264
Homologene: 130637
Grina
Name: glutamate receptor, ionotropic, N-methyl D-aspartate-associated protein 1 (glutamate binding)
Synonyms: 1110025J15Rik, Tmbim3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 66168
VEGA: 15
HGNC: HGNC:4589
Homologene: 41517
Avil
Name: advillin
Synonyms: DOC6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 11567
Homologene: 38200
Loxhd1
Name: lipoxygenase homology domains 1
Synonyms: 1700096C21Rik, sba
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 240411
Prdm1
Name: PR domain containing 1, with ZNF domain
Synonyms: PRDI-BF1, Blimp1, b2b1765Clo, Blimp-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 12142
HGNC: HGNC:9346
Homologene: 925
Trappc11
Name: trafficking protein particle complex 11
Synonyms: D030016E14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 320714
Homologene: 11076
Serpina3g
Name: serine (or cysteine) peptidase inhibitor, clade A, member 3G
Synonyms: alpha-1 antiproteinase, 2A2, Spi2/eb.1, Spi2-1, alpha-1 antiproteinase,, Spi2A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 20715
HGNC: HGNC:16
Homologene: 115927
Sema3a
Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A
Synonyms: semaphorin III, SemD, Semad, sema III, collapsin-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 20346
Homologene: 31358
Armc4
Name: armadillo repeat containing 4
Synonyms: b2b643Clo, 4930463I21Rik, b2b227.1Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 74934
VEGA: 18
Homologene: 9992
Sobp
Name: sine oculis binding protein
Synonyms: Jxc1, 5330439J01Rik, 2900009C16Rik, jc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 109205
Homologene: 41216
Cfap300
Name: cilia and flagella associated protein 300
Synonyms: 9230110C19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 234912
VEGA: 9
Homologene: 13163
CK137956
Name: cDNA sequence CK137956
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 635169
Homologene: 57187
Gpaa1
Name: GPI anchor attachment protein 1
Synonyms: mGAA1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 14731
HGNC: HGNC:4446
Homologene: 37852
Fsd2
Name: fibronectin type III and SPRY domain containing 2
Synonyms: 9830160G03Rik, Spryd1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 244091
Homologene: 18252
Pcdhb19
Name: protocadherin beta 19
Synonyms: PcdhbS, Pcdhb11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 93890
HGNC: HGNC:8683
Homologene: 128411
Nr1d2
Name: nuclear receptor subfamily 1, group D, member 2
Synonyms: RVR, Rev-erb beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 353187
HGNC: HGNC:7963
Homologene: 3763
Alg11
Name: asparagine-linked glycosylation 11 (alpha-1,2-mannosyltransferase)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 207958
Homologene: 68893
Vmn2r16
Name: vomeronasal 2, receptor 16
Synonyms: EG384220
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 384220
Homologene: 104825
Ip6k2
Name: inositol hexaphosphate kinase 2
Synonyms: Ihpk2, 1500005N04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 76500
Homologene: 56929
Zfp709
Name: zinc finger protein 709
Synonyms: GIOT-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 236193
Homologene: 136795
Skint11
Name: selection and upkeep of intraepithelial T cells 11
Synonyms: A630098G03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230623
Homologene: 136292
Adamts8
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 8
Synonyms: METH-2, METH2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 30806
HGNC: HGNC:224
Homologene: 5108
Slc22a28
Name: solute carrier family 22, member 28
Synonyms: Gm5631
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 434674
VEGA: 19
Homologene: 77136
Gm5174
Name: predicted gene 5174
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 382395
VEGA: 10
Homologene: 141143
Igkv15-103
Name: immunoglobulin kappa chain variable 15-103
Synonyms: Igk-V32
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 692169
Gm8989
Name: predicted gene 8989
Synonyms: Gm21911
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 668128
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 130,841,817 bp
  • A to G, chromosome 3 at 127,536,962 bp
  • C to A, chromosome 4 at 114,244,659 bp
  • A to T, chromosome 4 at 127,970,729 bp
  • A to T, chromosome 5 at 13,473,397 bp
  • T to C, chromosome 5 at 109,330,561 bp
  • G to T, chromosome 6 at 68,437,840 bp
  • T to A, chromosome 6 at 86,850,062 bp
  • T to C, chromosome 7 at 81,545,860 bp
  • T to C, chromosome 7 at 99,483,609 bp
  • T to A, chromosome 7 at 106,330,688 bp
  • T to A, chromosome 7 at 140,096,178 bp
  • T to C, chromosome 8 at 22,065,557 bp
  • T to C, chromosome 8 at 47,524,968 bp
  • C to T, chromosome 8 at 71,890,805 bp
  • A to T, chromosome 9 at 8,026,070 bp
  • G to T, chromosome 9 at 30,951,388 bp
  • G to A, chromosome 9 at 108,805,648 bp
  • C to T, chromosome 10 at 43,021,648 bp
  • T to A, chromosome 10 at 44,441,576 bp
  • A to G, chromosome 10 at 86,656,933 bp
  • C to T, chromosome 10 at 127,006,627 bp
  • G to A, chromosome 11 at 72,714,484 bp
  • C to T, chromosome 12 at 55,640,644 bp
  • G to T, chromosome 12 at 73,982,580 bp
  • A to T, chromosome 12 at 104,240,287 bp
  • T to C, chromosome 14 at 18,215,446 bp
  • T to A, chromosome 15 at 76,249,329 bp
  • A to C, chromosome 15 at 76,332,467 bp
  • C to A, chromosome 15 at 79,671,005 bp
  • A to G, chromosome 15 at 102,312,989 bp
  • T to C, chromosome 17 at 69,207,888 bp
  • A to T, chromosome 18 at 7,217,008 bp
  • A to T, chromosome 18 at 34,795,744 bp
  • T to A, chromosome 18 at 37,499,190 bp
  • A to T, chromosome 18 at 77,372,329 bp
  • T to A, chromosome 18 at 80,222,256 bp
  • A to G, chromosome 19 at 8,063,408 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4165 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041007-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.