Strain Name:
C57BL/6J-MtgxR4173Btlr/Mmmh
Stock Number:
041012-MU
Citation ID:
RRID:MMRRC_041012-MU
Other Names:
R4173 (G1), C57BL/6J-MtgxR4173Btlr
Major Collection:

Gene Information

Pigl
Name: phosphatidylinositol glycan anchor biosynthesis, class L
Synonyms: LOC327942
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 327942
HGNC: HGNC:8966
Homologene: 3159
Vps13c
Name: vacuolar protein sorting 13C
Synonyms: C230055H22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 320528
VEGA: 9
Homologene: 41188
Shroom3
Name: shroom family member 3
Synonyms: Shrm3, Shrm, D5Ertd287e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 27428
Homologene: 9263
Ubr1
Name: ubiquitin protein ligase E3 component n-recognin 1
Synonyms: E3 alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 22222
Homologene: 7582
Xrn2
Name: 5'-3' exoribonuclease 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 24128
Homologene: 6927
Copa
Name: coatomer protein complex subunit alpha
Synonyms: xenin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 12847
HGNC: HGNC:2230
Homologene: 3218
Prss16
Name: protease, serine 16 (thymus)
Synonyms: TSSP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 54373
HGNC: HGNC:9480
Homologene: 38106
Txnl4b
Name: thioredoxin-like 4B
Synonyms: Dim2, D530025J19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 234723
Homologene: 9880
Cdk1
Name: cyclin-dependent kinase 1
Synonyms: p34CDC2, Cdc2a, Cdc2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 12534
HGNC: HGNC:1722
Homologene: 68203
Pip4k2b
Name: phosphatidylinositol-5-phosphate 4-kinase, type II, beta
Synonyms: PI5P4Kbeta, c11, Pip5k2b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 108083
HGNC: HGNC:8998
Homologene: 2634
Nr1h5
Name: nuclear receptor subfamily 1, group H, member 5
Synonyms: FXRB
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 381463
Homologene: 131277
Slc25a45
Name: solute carrier family 25, member 45
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 107375
Homologene: 76679
Cspg4
Name: chondroitin sulfate proteoglycan 4
Synonyms: NG2, AN2, 4732461B14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 121021
VEGA: 9
HGNC: HGNC:2466
Homologene: 20445
Thbs2
Name: thrombospondin 2
Synonyms: Thrombospondin-2, Thbs-2, TSP2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 21826
VEGA: 17
Homologene: 2438
Smgc
Name: submandibular gland protein C
Synonyms: Sfc21, DXImx49e, 2310010P21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 223809
Rora
Name: RAR-related orphan receptor alpha
Synonyms: tmgc26, 9530021D13Rik, Nr1f1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 19883
Homologene: 56594
Trim28
Name: tripartite motif-containing 28
Synonyms: MommeD9, KRIP-1, Tif1b, KAP-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 21849
Homologene: 21175
Myo5c
Name: myosin VC
Synonyms: 9130003O20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 208943
VEGA: 9
HGNC: HGNC:7604
Homologene: 135711
Xkr4
Name: X-linked Kx blood group related 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 497097
Homologene: 45848
Serpinb9e
Name: serine (or cysteine) peptidase inhibitor, clade B, member 9e
Synonyms: NK26, Spi14, ovalbumin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 20710
HGNC: HGNC:8955
Homologene: 69093
Opcml
Name: opioid binding protein/cell adhesion molecule-like
Synonyms: B930023M13Rik, 2900075O15Rik, Obcam, LOC235104
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 330908
HGNC: HGNC:8143
Homologene: 55663
Gnmt
Name: glycine N-methyltransferase
Synonyms: glycine N methyl transferase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 14711
HGNC: HGNC:4415
Homologene: 7741
Olfr48
Name: olfactory receptor 48
Synonyms: MOR232-5, GA_x6K02T2Q125-51285881-51284976, IC3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 18347
Homologene: 85936
Timd2
Name: T cell immunoglobulin and mucin domain containing 2
Synonyms: TIM-2, Tim2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 171284
Homologene: 82236
Pcdha9
Name: protocadherin alpha 9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 192161
HGNC: HGNC:8664
Homologene: 130700
Htra2
Name: HtrA serine peptidase 2
Synonyms: Prss25, OMI, mnd2, HtrA2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 64704
Homologene: 113300
Rpl31-ps17
Name: ribosomal protein L31, pseudogene 17
Synonyms: Gm16382
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 673582
Trav6d-3
Name: T cell receptor alpha variable 6D-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 627394
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 3,216,488 bp
  • T to C, chromosome 1 at 172,097,702 bp
  • T to G, chromosome 2 at 89,844,778 bp
  • C to T, chromosome 2 at 120,946,622 bp
  • T to C, chromosome 2 at 147,047,692 bp
  • C to T, chromosome 3 at 102,952,230 bp
  • G to T, chromosome 5 at 92,943,086 bp
  • C to T, chromosome 6 at 83,054,064 bp
  • A to G, chromosome 7 at 13,029,878 bp
  • G to A, chromosome 8 at 109,569,074 bp
  • C to T, chromosome 9 at 28,903,358 bp
  • A to G, chromosome 9 at 56,887,930 bp
  • A to G, chromosome 9 at 67,936,313 bp
  • C to A, chromosome 9 at 68,653,910 bp
  • A to G, chromosome 9 at 75,246,258 bp
  • T to C, chromosome 10 at 69,345,161 bp
  • T to C, chromosome 11 at 46,670,960 bp
  • T to A, chromosome 11 at 62,458,511 bp
  • T to C, chromosome 11 at 97,722,375 bp
  • C to T, chromosome 12 at 54,701,612 bp
  • A to G, chromosome 13 at 22,003,183 bp
  • A to G, chromosome 13 at 33,255,158 bp
  • A to G, chromosome 14 at 52,725,349 bp
  • T to C, chromosome 15 at 91,860,561 bp
  • A to C, chromosome 17 at 14,681,631 bp
  • A to G, chromosome 17 at 46,726,121 bp
  • T to C, chromosome 18 at 37,012,623 bp
  • C to A, chromosome 19 at 5,880,583 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4173 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041012-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.