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Strain Name:
C57BL/6J-MtgxR4183Btlr/Mmmh
Stock Number:
041019-MU
Citation ID:
RRID:MMRRC_041019-MU
Other Names:
R4183 (G1), C57BL/6J-MtgxR4183Btlr
Major Collection:

Strain Information

Mapk8
Name: mitogen-activated protein kinase 8
Synonyms: JNK1, c-Jun N-terminal kinase, Prkm8
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 26419
HGNC: HGNC:6881
Homologene: 56760
Bicral
Name: BRD4 interacting chromatin remodeling complex associated protein like
Synonyms: BC032203, Gltscr1l
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 210982
VEGA: 17
Homologene: 19366
Arhgap12
Name: Rho GTPase activating protein 12
Synonyms: 2810011M08Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 75415
Homologene: 23089
Ddx1
Name: DEAD box helicase 1
Synonyms: DEAD (Asp-Glu-Ala-Asp) box polypeptide 1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 104721
VEGA: 12
HGNC: HGNC:2734
Homologene: 3627
Ankrd11
Name: ankyrin repeat domain 11
Synonyms: 2410104C19Rik, 9530048I21Rik, 3010027A04Rik, Yod
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 77087
Homologene: 69134
Ranbp2
Name: RAN binding protein 2
Synonyms: A430087B05Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 19386
VEGA: 10
HGNC: HGNC:9848
Homologene: 87808
Arpc2
Name: actin related protein 2/3 complex, subunit 2
Synonyms: 2210023N03Rik, p34-Arc
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 76709
HGNC: HGNC:705
Homologene: 4187
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 20,117,807 bp
  • T to A, chromosome 1 at 74,248,163 bp
  • T to C, chromosome 1 at 160,097,355 bp
  • T to A, chromosome 1 at 173,875,771 bp
  • C to A, chromosome 2 at 11,718,487 bp
  • A to G, chromosome 2 at 32,774,444 bp
  • A to G, chromosome 2 at 76,711,242 bp
  • T to C, chromosome 2 at 89,406,591 bp
  • A to G, chromosome 2 at 103,739,813 bp
  • A to G, chromosome 2 at 120,017,865 bp
  • G to A, chromosome 2 at 177,921,457 bp
  • T to A, chromosome 4 at 41,764,981 bp
  • G to A, chromosome 4 at 80,953,858 bp
  • T to C, chromosome 4 at 134,529,247 bp
  • A to G, chromosome 4 at 147,512,693 bp
  • TTCCTCCTCCTCCTCCTCCTC to TTCCTCCTCCTCCTCCTC, chromosome 4 at 154,036,306 bp
  • G to A, chromosome 5 at 88,664,156 bp
  • C to A, chromosome 6 at 3,963,926 bp
  • A to G, chromosome 7 at 87,001,891 bp
  • A to G, chromosome 7 at 89,851,830 bp
  • T to C, chromosome 7 at 103,670,971 bp
  • T to C, chromosome 7 at 118,984,526 bp
  • T to C, chromosome 8 at 11,502,757 bp
  • T to A, chromosome 8 at 12,816,990 bp
  • C to T, chromosome 8 at 15,910,464 bp
  • A to G, chromosome 8 at 71,710,410 bp
  • T to C, chromosome 8 at 122,899,676 bp
  • A to G, chromosome 9 at 39,737,048 bp
  • A to T, chromosome 9 at 110,636,675 bp
  • T to C, chromosome 10 at 58,465,666 bp
  • C to T, chromosome 10 at 80,325,011 bp
  • A to G, chromosome 11 at 3,501,134 bp
  • T to C, chromosome 11 at 67,349,610 bp
  • T to C, chromosome 11 at 69,787,096 bp
  • A to T, chromosome 12 at 13,231,503 bp
  • T to C, chromosome 12 at 108,692,099 bp
  • T to A, chromosome 12 at 109,027,331 bp
  • A to G, chromosome 13 at 22,474,852 bp
  • A to G, chromosome 13 at 112,994,372 bp
  • T to C, chromosome 14 at 33,382,220 bp
  • C to G, chromosome 14 at 50,681,050 bp
  • T to C, chromosome 14 at 63,452,320 bp
  • A to G, chromosome 14 at 73,198,526 bp
  • A to T, chromosome 15 at 74,588,127 bp
  • G to A, chromosome 15 at 99,933,685 bp
  • A to G, chromosome 17 at 29,969,990 bp
  • T to G, chromosome 17 at 37,231,848 bp
  • T to C, chromosome 17 at 46,814,029 bp
  • T to C, chromosome 18 at 6,111,734 bp
  • G to C, chromosome 18 at 37,763,541 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4183 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041019-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.