Strain Name:
C57BL/6J-MtgxR4199Btlr/Mmmh
Stock Number:
041029-MU
Citation ID:
RRID:MMRRC_041029-MU
Other Names:
R4199 (G1), C57BL/6J-MtgxR4199Btlr
Major Collection:

Strain Information

Pkd1
Name: polycystin 1, transient receptor potential channel interacting
Synonyms: PC1, polycystin-1, PC-1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18763
VEGA: 17
HGNC: HGNC:9008
Homologene: 250
Pknox1
Name: Pbx/knotted 1 homeobox
Synonyms: PREP1, D17Wsu76e
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18771
HGNC: HGNC:9022
Homologene: 3363
Xpnpep1
Name: X-prolyl aminopeptidase (aminopeptidase P) 1, soluble
Synonyms: D230045I08Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 170750
Homologene: 6424
Dnmt1
Name: DNA methyltransferase 1
Synonyms: Cxxc9, Dnmt1o, MommeD2, MTase
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 13433
VEGA: 9
HGNC: HGNC:2976
Homologene: 124071
Eps8
Name: epidermal growth factor receptor pathway substrate 8
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 13860
HGNC: HGNC:3420
Homologene: 3272
Orc4
Name: origin recognition complex, subunit 4
Synonyms: Orc4l, mMmORC4, Orc4P, Orc4
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 26428
HGNC: HGNC:8490
Homologene: 8059
Gga1
Name: golgi associated, gamma adaptin ear containing, ARF binding protein 1
Synonyms: 4930406E12Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 106039
VEGA: 15
Homologene: 39250
Sfxn5
Name: sideroflexin 5
Synonyms: C230001H08Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 94282
Homologene: 16936
Cic
Name: capicua transcriptional repressor
Synonyms: 1200010B10Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 71722
Homologene: 87637
Myh14
Name: myosin, heavy polypeptide 14
Synonyms: NMHC II-C, 2400004E04Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 71960
Homologene: 23480
Il17rb
Name: interleukin 17 receptor B
Synonyms: IL-17Rh1, IL-17ER, Evi27, IL17RH1, Il17br
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 50905
Homologene: 10287
Nol8
Name: nucleolar protein 8
Synonyms: D13Ertd548e, 5730412B09Rik, 4921532D18Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 70930
VEGA: 13
Homologene: 41210
Eml2
Name: echinoderm microtubule associated protein like 2
Synonyms: 1600029N02Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 72205
Homologene: 8125
Fbxo34
Name: F-box protein 34
Synonyms: 5830426G16Rik, 2900057B08Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 78938
VEGA: 14
Homologene: 12713
Lcorl
Name: ligand dependent nuclear receptor corepressor-like
Synonyms: Mlr1, Gm3414, A830039H10Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100041576
Homologene: 82325
Papln
Name: papilin, proteoglycan-like sulfated glycoprotein
Synonyms: E030033C16Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 170721
Homologene: 71541
Rpl5
Name: ribosomal protein L5
Synonyms: U21RNA, Skam23Jus, Skax23, Ska23
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100503670
Homologene: 110649
Selenop
Name: selenoprotein P
Synonyms: D15Ucla1, Sepp1, Se-P, selp
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 20363
Homologene: 3945
Slc1a1
Name: solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1
Synonyms: EAAT3, MEAAC1, D130048G10Rik, EAAC1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 20510
Homologene: 20881
Dnah3
Name: dynein, axonemal, heavy chain 3
Synonyms: Dnahc3
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 381917
HGNC: HGNC:2949
Homologene: 19674
Ces1f
Name: carboxylesterase 1F
Synonyms: TGH-2, CesML1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234564
HGNC: HGNC:1863
Homologene: 134311
Ankhd1
Name: ankyrin repeat and KH domain containing 1
Synonyms: 1110004O12Rik, A530027J04Rik, 4933432B13Rik, 9130019P20Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 108857
Homologene: 87006
Zfp276
Name: zinc finger protein (C2H2 type) 276
Synonyms: D8Ertd377e, D8Ertd370e
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 57247
Homologene: 10731
Spef2
Name: sperm flagellar 2
Synonyms: C230086A09Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 320277
Homologene: 23371
Or1i2
Name: olfactory receptor family 1 subfamily I member 1
Synonyms: GA_x6K02T2QGN0-3196801-3197742, MOR128-4, MOR128-3, Olfr1357
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 257883
HGNC: HGNC:8207
Homologene: 72920
Serpinb9d
Name: serine (or cysteine) peptidase inhibitor, clade B, member 9d
Synonyms: Spi9, ovalbumin, AT2
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 20726
HGNC: HGNC:8955
Homologene: 69093
Ifitm5
Name: interferon induced transmembrane protein 5
Synonyms: 1110003J06Rik, Bril, Hrmp1, fragilis4
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 73835
Homologene: 14210
Foxg1
Name: forkhead box G1
Synonyms: Hfh9, 2900064B05Rik, BF-1, Hfhbf1, Bf1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 15228
HGNC: HGNC:3811
Homologene: 3843
Disc1
Name: disrupted in schizophrenia 1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 244667
HGNC: HGNC:2888
Homologene: 10257
Cdca7
Name: cell division cycle associated 7
Synonyms: 2310021G01Rik, JPO1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 66953
Homologene: 49970
Stx5a
Name: syntaxin 5A
Synonyms: syntaxin 5, D19Ertd627e, 0610031F24Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 56389
Homologene: 2381
Cbarp
Name: calcium channel, voltage-dependent, beta subunit associated regulatory protein
Synonyms: Dos, R29144/1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 100503659
Homologene: 82309
Gal3st3
Name: galactose-3-O-sulfotransferase 3
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 545276
VEGA: 19
Homologene: 57198
Irf2bp2
Name: interferon regulatory factor 2 binding protein 2
Synonyms: E130305N23Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 270110
Homologene: 34290
Gm24157
Name: predicted gene, 24157
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Ighg2b
Name: immunoglobulin heavy constant gamma 2B
Synonyms: IgG2b, gamma2b
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 16016
Syp
Name: synaptophysin
Synonyms: Syn, A230093K24Rik, p38, Syp I
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 20977
Homologene: 2391
Pabir3
Name: PABIR family member 3
Synonyms: Fam122c, 4930432H15Rik, 4930527G05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 73866
Homologene: 87062
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 2 at 25,824,761 bp
  • G to A, chromosome 2 at 48,937,489 bp
  • C to T, chromosome 2 at 72,481,843 bp
  • G to A, chromosome 3 at 55,047,315 bp
  • T to C, chromosome 5 at 45,733,788 bp
  • T to C, chromosome 5 at 107,902,125 bp
  • T to A, chromosome 6 at 85,215,742 bp
  • T to C, chromosome 6 at 137,514,327 bp
  • G to A, chromosome 7 at 19,179,439 bp
  • TCCCCC to TCCCCCCC, chromosome 7 at 25,291,670 bp
  • T to A, chromosome 7 at 44,615,503 bp
  • C to T, chromosome 7 at 119,922,838 bp
  • T to A, chromosome 7 at 140,949,236 bp
  • A to G, chromosome 8 at 93,256,889 bp
  • A to G, chromosome 8 at 123,267,825 bp
  • C to T, chromosome 8 at 125,148,459 bp
  • C to A, chromosome 8 at 126,591,574 bp
  • A to G, chromosome 9 at 20,938,118 bp
  • A to T, chromosome 10 at 78,612,067 bp
  • G to T, chromosome 10 at 80,135,492 bp
  • A to G, chromosome 11 at 52,099,101 bp
  • T to A, chromosome 12 at 49,385,299 bp
  • C to T, chromosome 12 at 83,783,392 bp
  • G to T, chromosome 12 at 113,307,287 bp
  • A to G, chromosome 13 at 33,202,674 bp
  • T to A, chromosome 13 at 49,661,748 bp
  • C to T, chromosome 14 at 29,996,644 bp
  • T to A, chromosome 14 at 47,530,997 bp
  • C to A, chromosome 15 at 3,274,718 bp
  • A to G, chromosome 15 at 9,667,280 bp
  • A to G, chromosome 15 at 78,889,075 bp
  • A to G, chromosome 17 at 24,570,030 bp
  • C to A, chromosome 17 at 31,602,816 bp
  • C to G, chromosome 18 at 36,661,048 bp
  • T to A, chromosome 19 at 5,307,780 bp
  • A to T, chromosome 19 at 8,743,474 bp
  • A to G, chromosome 19 at 28,901,452 bp
  • G to A, chromosome 19 at 52,996,973 bp
  • A to G, chromosome X at 7,639,927 bp
  • G to A, chromosome X at 53,293,499 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4199 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041029-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.