Strain Name:
C57BL/6J-MtgxR4204Btlr/Mmmh
Stock Number:
041033-MU
Citation ID:
RRID:MMRRC_041033-MU
Other Names:
R4204 (G1), C57BL/6J-MtgxR4204Btlr
Major Collection:

Strain Information

Hgs
Name: HGF-regulated tyrosine kinase substrate
Synonyms: Hrs, tn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 15239
HGNC: HGNC:4897
Homologene: 37954
Mrtfb
Name: myocardin related transcription factor B
Synonyms: MRTF-B, Gt4-1, Mrtfb, Mkl2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 239719
Homologene: 40917
Mindy3
Name: MINDY lysine 48 deubiquitinase 3
Synonyms: 1810041E18Rik, 5830410F13Rik, 2310047O13Rik, Fam188a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 66960
Homologene: 11478
Ubxn2a
Name: UBX domain protein 2A
Synonyms: 6330407P03Rik, Ubxd4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 217379
Homologene: 17076
Npy5r
Name: neuropeptide Y receptor Y5
Synonyms: Y5R
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 18168
HGNC: HGNC:7958
Homologene: 21241
Lgals9
Name: lectin, galactose binding, soluble 9
Synonyms: LGALS35, galectin-9, gal-9, Lgals5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 16859
Homologene: 134662
Shroom3
Name: shroom family member 3
Synonyms: D5Ertd287e, Shrm, Shrm3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 27428
Homologene: 9263
Pdxdc1
Name: pyridoxal-dependent decarboxylase domain containing 1
Synonyms: 2210010A19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 94184
Homologene: 22858
Mtcl1
Name: microtubule crosslinking factor 1
Synonyms: t8219b25, 1110012J17Rik, Soga2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 68617
Homologene: 41017
Resf1
Name: retroelement silencing factor 1
Synonyms: GET, 2810474O19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 67246
Homologene: 19251
Pex14
Name: peroxisomal biogenesis factor 14
Synonyms: Pex14p
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 56273
HGNC: HGNC:8856
Homologene: 37936
Kank2
Name: KN motif and ankyrin repeat domains 2
Synonyms: Ankrd25
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235041
VEGA: 9
Homologene: 9163
Abca1
Name: ATP-binding cassette, sub-family A (ABC1), member 1
Synonyms: ABC1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 11303
HGNC: HGNC:29
Homologene: 21130
Ccdc171
Name: coiled-coil domain containing 171
Synonyms: 4930418J05Rik, A330015D16Rik, 4930473A06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 320226
Homologene: 27942
Ccdc88a
Name: coiled coil domain containing 88A
Synonyms: C330012F17Rik, D130005J21Rik, C130096N06Rik, GIV, A430106J12Rik, HkRP1, Girdin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 108686
Homologene: 41180
Tcaim
Name: T cell activation inhibitor, mitochondrial
Synonyms: LOC382117, D9Ertd402e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 382117
Homologene: 45481
Trpm3
Name: transient receptor potential cation channel, subfamily M, member 3
Synonyms: B930001P07Rik, 6330504P12Rik, MLSN2, melastatin 2, LTRPC3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 226025
VEGA: 19
Homologene: 62287
Itga4
Name: integrin alpha 4
Synonyms: VLA-4 receptor, alpha 4 subunit
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 16401
HGNC: HGNC:6140
Homologene: 37364
Sis
Name: sucrase isomaltase (alpha-glucosidase)
Synonyms: Si-s, sucrase-isomaltase, 2010204N08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 69983
Homologene: 37424
Hcn3
Name: hyperpolarization-activated, cyclic nucleotide-gated K+ 3
Synonyms: Hac3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 15168
Homologene: 22453
Kprp
Name: keratinocyte expressed, proline-rich
Synonyms: 1110001M24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 433619
Homologene: 54921
Sacs
Name: sacsin
Synonyms: E130115J16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 50720
Or4c120
Name: olfactory receptor family 4 subfamily C member 120
Synonyms: GA_x6K02T2Q125-50650037-50649102, MOR233-11, Olfr1225
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258893
Homologene: 17427
Tmem132c
Name: transmembrane protein 132C
Synonyms: 4632425D07Rik, 2810482M11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 208213
Homologene: 76567
Fam83b
Name: family with sequence similarity 83, member B
Synonyms: C530008M07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 208994
Homologene: 19478
Prodh
Name: proline dehydrogenase
Synonyms: Pro-1, Pro1, Ym24d07
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 19125
HGNC: HGNC:9453
Homologene: 40764
BC028528
Name: cDNA sequence BC028528
Synonyms: L259
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229600
Homologene: 49773
B130006D01Rik
Name: RIKEN cDNA B130006D01 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 320616
VEGA: 11
Zfp800
Name: zinc finger protein 800
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 627049
Homologene: 18529
Nhej1
Name: non-homologous end joining factor 1
Synonyms: 1700029B21Rik, XLF, cernunnos
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 75570
Homologene: 11714
Pde8b
Name: phosphodiesterase 8B
Synonyms: B230331L10Rik, C030047E14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 218461
HGNC: HGNC:8794
Homologene: 2758
Rasgef1c
Name: RasGEF domain family, member 1C
Synonyms: 9130006A14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 74563
Homologene: 17918
Rgl2
Name: ral guanine nucleotide dissociation stimulator-like 2
Synonyms: Rlf, KE1.5, Rab2l, Rgt2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 19732
HGNC: HGNC:9769
Homologene: 3494
Ano7
Name: anoctamin 7
Synonyms: IPCA-5, NGEP, NGEP-L, Pcanap5, Tmem16g
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 404545
Homologene: 45787
Mfrp
Name: membrane frizzled-related protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 259172
Homologene: 12866
Rpe65
Name: retinal pigment epithelium 65
Synonyms: A930029L06Rik, Mord1, rd12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 19892
Homologene: 20108
Mfsd9
Name: major facilitator superfamily domain containing 9
Synonyms: 4931419K03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 211798
Homologene: 13100
Utp14b
Name: UTP14B small subunit processome component
Synonyms: jsd, 4932411L21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 195434
Homologene: 78703
Rnf133
Name: ring finger protein 133
Synonyms: Greul2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 386611
Homologene: 44549
Ppp2r2b
Name: protein phosphatase 2, regulatory subunit B, beta
Synonyms: 6330404L05Rik, PP2A-PR55B, PR55-BETA, SCA12, 2900026H06Rik, E130009M08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 72930
HGNC: HGNC:9305
Homologene: 55833
Tex11
Name: testis expressed gene 11
Synonyms: 4930565P14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 83558
Homologene: 49962
Serp2
Name: stress-associated endoplasmic reticulum protein family member 2
Synonyms: 2810032E02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 72661
VEGA: 14
Homologene: 84638
Pcdha8
Name: protocadherin alpha 8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 353235
Homologene: 129614
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 40,781,510 bp
  • A to T, chromosome 1 at 75,046,623 bp
  • G to A, chromosome 1 at 78,664,822 bp
  • A to G, chromosome 1 at 93,380,478 bp
  • A to G, chromosome 2 at 12,405,900 bp
  • T to C, chromosome 2 at 79,279,161 bp
  • A to G, chromosome 2 at 89,170,780 bp
  • T to C, chromosome 3 at 72,961,082 bp
  • A to G, chromosome 3 at 89,148,844 bp
  • A to C, chromosome 3 at 92,824,739 bp
  • A to T, chromosome 3 at 95,889,745 bp
  • G to A, chromosome 3 at 159,604,410 bp
  • T to C, chromosome 4 at 53,090,369 bp
  • A to T, chromosome 4 at 83,681,155 bp
  • T to C, chromosome 4 at 148,963,527 bp
  • G to T, chromosome 5 at 92,943,086 bp
  • A to G, chromosome 5 at 127,563,765 bp
  • T to C, chromosome 6 at 23,649,049 bp
  • C to T, chromosome 6 at 28,243,181 bp
  • C to T, chromosome 6 at 149,329,544 bp
  • G to T, chromosome 8 at 66,682,041 bp
  • A to G, chromosome 9 at 21,795,627 bp
  • G to A, chromosome 9 at 44,105,228 bp
  • T to C, chromosome 9 at 76,503,053 bp
  • A to G, chromosome 9 at 122,833,618 bp
  • A to G, chromosome 11 at 29,463,399 bp
  • G to A, chromosome 11 at 49,958,708 bp
  • C to T, chromosome 11 at 78,969,816 bp
  • A to G, chromosome 11 at 95,726,424 bp
  • C to A, chromosome 11 at 120,477,187 bp
  • T to C, chromosome 12 at 4,894,593 bp
  • C to A, chromosome 13 at 95,222,545 bp
  • A to G, chromosome 14 at 61,173,443 bp
  • A to G, chromosome 14 at 76,556,462 bp
  • A to T, chromosome 16 at 13,403,255 bp
  • T to C, chromosome 16 at 13,879,482 bp
  • A to G, chromosome 16 at 18,072,318 bp
  • G to T, chromosome 17 at 33,936,932 bp
  • T to C, chromosome 17 at 66,438,261 bp
  • G to C, chromosome 18 at 36,994,684 bp
  • T to A, chromosome 18 at 42,738,050 bp
  • T to A, chromosome 19 at 22,987,564 bp
  • C to A, chromosome X at 100,933,415 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4204 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041033-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.