Strain Name:
C57BL/6J-MtgxR4222Btlr/Mmmh
Stock Number:
041042-MU
Citation ID:
RRID:MMRRC_041042-MU
Other Names:
R4222 (G1), C57BL/6J-MtgxR4222Btlr
Major Collection:

Strain Information

Ptx3
Name: pentraxin related gene
Synonyms: TSG-14, pentraxin 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 19288
HGNC: HGNC:9692
Homologene: 7500
Ptch1
Name: patched 1
Synonyms: Ptc, Patched 1, Ptc1, A230106A15Rik, wig
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 19206
HGNC: HGNC:9585
Homologene: 223
Atm
Name: ataxia telangiectasia mutated
Synonyms: C030026E19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 11920
HGNC: HGNC:795
Homologene: 30952
Pkn2
Name: protein kinase N2
Synonyms: PRK2, 6030436C20Rik, Stk7, Prkcl2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 109333
HGNC: HGNC:9406
Homologene: 2054
Ptbp1
Name: polypyrimidine tract binding protein 1
Synonyms: hnRNP I, Ptb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 19205
HGNC: HGNC:9583
Homologene: 49188
Zfp35
Name: zinc finger protein 35
Synonyms: Zfp-35
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 22694
Homologene: 133081
Ryr2
Name: ryanodine receptor 2, cardiac
Synonyms: 9330127I20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 20191
VEGA: 13
Homologene: 37423
Kri1
Name: KRI1 homolog
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 215194
Homologene: 133742
Tbc1d14
Name: TBC1 domain family, member 14
Synonyms: 2810413P16Rik, D5Ertd110e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100855
Homologene: 10809
Alkal1
Name: ALK and LTK ligand 1
Synonyms: EG620393, Fam150a, Augmentor beta, Augbeta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 620393
Homologene: 82437
Spag5
Name: sperm associated antigen 5
Synonyms: MAP126, Astrin, s17, S17, Mastrin, Deepest, D11Bhm180e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 54141
Homologene: 4718
Ptk7
Name: PTK7 protein tyrosine kinase 7
Synonyms: 8430404F20Rik, mPTK7/CCK4, chz
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 71461
VEGA: 17
HGNC: HGNC:9618
Homologene: 43672
Gm7964
Name: predicted gene 7964
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 666170
Olfm3
Name: olfactomedin 3
Synonyms: optimedin, B230206G02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229759
Homologene: 17103
Rsrc1
Name: arginine/serine-rich coiled-coil 1
Synonyms: SRrp53, 1200013F24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 66880
Homologene: 41147
Gar1
Name: GAR1 ribonucleoprotein
Synonyms: GAR1, C430047J18Rik, Nola1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 68147
Trerf1
Name: transcriptional regulating factor 1
Synonyms: Trep132, Trep-132, 9430096I18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224829
Homologene: 14129
Parm1
Name: prostate androgen-regulated mucin-like protein 1
Synonyms: 2210012L08Rik, 9130213B05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231440
Homologene: 41036
Aif1l
Name: allograft inflammatory factor 1-like
Synonyms: 2810003C17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 108897
Homologene: 12863
Myo7a
Name: myosin VIIA
Synonyms: Myo7, USH1B, nmf371, polka, Hdb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 17921
HGNC: HGNC:7606
Homologene: 219
Zbtb5
Name: zinc finger and BTB domain containing 5
Synonyms: 9430083K24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230119
Homologene: 8913
Lama3
Name: laminin, alpha 3
Synonyms: nicein, 150kDa, [a]3B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 16774
HGNC: HGNC:6483
Homologene: 18279
9930021J03Rik
Name: RIKEN cDNA 9930021J03 gene
Synonyms: Gm9832
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 240613
Homologene: 19046
Slc25a45
Name: solute carrier family 25, member 45
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 107375
Homologene: 76679
Nebl
Name: nebulette
Synonyms: Lnebl, 1200007O21Rik, A630080F05Rik, D830029A09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 74103
Homologene: 128431
Adgre4
Name: adhesion G protein-coupled receptor E4
Synonyms: EGF-TM7, D17Ertd479e, FIRE, Gpr127, Emr4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 52614
VEGA: 17
Homologene: 64640
Nrxn3
Name: neurexin III
Synonyms: neurexin III beta, neurexin III alpha, neurexin III beta, neurexin III alpha, 9330112C09Rik, D12Bwg0831e, 4933401A11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 18191
HGNC: HGNC:8010
Homologene: 83225
Tlr11
Name: toll-like receptor 11
Synonyms: LOC239081
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 239081
Homologene: 77905
Hapln4
Name: hyaluronan and proteoglycan link protein 4
Synonyms: Bral2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 330790
Homologene: 11304
Mrpl22
Name: mitochondrial ribosomal protein L22
Synonyms: MRP-L25, Rpml25, HSPC158, E030011D16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216767
Homologene: 56664
Nipsnap3a
Name: nipsnap homolog 3A
Synonyms: 1700054F22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 73398
Homologene: 136268
Vmn2r60
Name: vomeronasal 2, receptor 60
Synonyms: Casr-rs3, EG637898, Gprc2a-rs3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 637898
Homologene: 129683
Vmn2r14
Name: vomeronasal 2, receptor 14
Synonyms: EG231591
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231591
Homologene: 129606
Ttc26
Name: tetratricopeptide repeat domain 26
Synonyms: hpy, hydrocephalic-polydactyl, 9430097H08Rik, hop
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 264134
Homologene: 11786
Cyp3a11
Name: cytochrome P450, family 3, subfamily a, polypeptide 11
Synonyms: IIIAm1, Pcn, Cyp3a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 13112
Homologene: 133568
Bhmt-ps1
Name: betaine-homocysteine methyltransferase, pseudogene 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 57421
Or8u8
Name: olfactory receptor family 8 subfamily U member 8
Synonyms: IE6, MOR185-6, GA_x6K02T2Q125-47650922-47649963, Olfr52
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 18352
Homologene: 105200
Trim43b
Name: tripartite motif-containing 43B
Synonyms: Gm8269
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 666747
Homologene: 105432
Vmn1r49
Name: vomeronasal 1, receptor 49
Synonyms: VRi2, V1r5, V1rb2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 24112
Homologene: 113975
Or52z12
Name: olfactory receptor family 52 subfamily Z member 12
Synonyms: GA_x6K02T2PBJ9-6306819-6307775, MOR31-10, Olfr617
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258838
Homologene: 105208
Ston1
Name: stonin 1
Synonyms: 4921524J06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 77057
Homologene: 122152
Semp2l2a
Name: SUMO/sentrin specific peptidase 2-like 2A
Synonyms: AF366264
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 231201
Homologene: 130042
Rrp8
Name: ribosomal RNA processing 8
Synonyms: 2900001K19Rik, 1500003O22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 101867
Homologene: 5621
H1f5
Name: H1.5 linker histone, cluster member
Synonyms: H1B, H1.5, H1s-3, H1f5, Hist1h1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 56702
HGNC: HGNC:4719
Homologene: 135981
Phc3
Name: polyhomeotic 3
Synonyms: EDR3, HPH3, E030046K01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 241915
Homologene: 69390
Gm5265
Name: predicted pseudogene 5265
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 383613
Grin1os
Name: glutamate receptor, ionotropic, NMDA1 (zeta 1), opposite strand
Synonyms: E130003G02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 320354
Irgq
Name: immunity-related GTPase family, Q
Synonyms: FKSG27
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 210146
Homologene: 17696
Gm5327
Name: predicted pseudogene 5327
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 384591
Vmn1r-ps103
Name: vomeronasal 1 receptor, pseudogene 103
Synonyms: V1rh1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 171245
Homologene: 110880
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 6,388,615 bp
  • A to T, chromosome 1 at 169,453,801 bp
  • G to A, chromosome 2 at 17,443,778 bp
  • T to C, chromosome 2 at 25,297,320 bp
  • A to T, chromosome 2 at 31,962,239 bp
  • G to T, chromosome 2 at 86,181,997 bp
  • A to T, chromosome 3 at 30,936,819 bp
  • C to T, chromosome 3 at 66,224,706 bp
  • A to G, chromosome 3 at 66,994,567 bp
  • C to T, chromosome 3 at 115,090,171 bp
  • A to T, chromosome 3 at 129,830,806 bp
  • A to T, chromosome 3 at 142,793,866 bp
  • T to A, chromosome 4 at 26,369,352 bp
  • A to G, chromosome 4 at 44,993,855 bp
  • T to A, chromosome 4 at 52,997,251 bp
  • A to T, chromosome 5 at 36,493,108 bp
  • G to A, chromosome 5 at 91,593,867 bp
  • A to G, chromosome 5 at 109,216,283 bp
  • A to T, chromosome 5 at 145,860,466 bp
  • A to G, chromosome 6 at 38,395,075 bp
  • A to G, chromosome 6 at 90,072,246 bp
  • C to A, chromosome 7 at 24,533,625 bp
  • A to T, chromosome 7 at 32,546,617 bp
  • A to T, chromosome 7 at 42,116,528 bp
  • T to G, chromosome 7 at 83,756,822 bp
  • T to A, chromosome 7 at 98,073,229 bp
  • A to G, chromosome 7 at 103,584,759 bp
  • T to C, chromosome 7 at 105,734,022 bp
  • T to C, chromosome 8 at 13,838,061 bp
  • T to A, chromosome 8 at 70,086,960 bp
  • T to C, chromosome 9 at 21,281,063 bp
  • T to C, chromosome 9 at 53,480,669 bp
  • T to A, chromosome 9 at 89,090,639 bp
  • T to C, chromosome 10 at 79,859,213 bp
  • T to A, chromosome 11 at 58,171,867 bp
  • G to A, chromosome 11 at 78,304,511 bp
  • G to T, chromosome 12 at 89,532,992 bp
  • T to A, chromosome 13 at 11,737,873 bp
  • A to T, chromosome 13 at 21,779,977 bp
  • C to A, chromosome 13 at 22,442,028 bp
  • C to T, chromosome 13 at 63,534,329 bp
  • C to T, chromosome 14 at 50,361,849 bp
  • C to T, chromosome 15 at 76,177,319 bp
  • A to T, chromosome 17 at 46,574,463 bp
  • G to T, chromosome 17 at 47,314,801 bp
  • A to G, chromosome 17 at 55,785,121 bp
  • A to G, chromosome 17 at 88,636,771 bp
  • T to C, chromosome 18 at 12,450,403 bp
  • T to G, chromosome 18 at 24,003,189 bp
  • A to T, chromosome 19 at 5,880,118 bp
  • A to T, chromosome 19 at 29,718,749 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4222 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041042-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.