Strain Name:
C57BL/6J-MtgxR4227Btlr/Mmmh
Stock Number:
041047-MU
Citation ID:
RRID:MMRRC_041047-MU
Other Names:
R4227 (G1), C57BL/6J-MtgxR4227Btlr
Major Collection:

Gene Information

Spen
Name: spen family transcription repressor
Synonyms: Mint
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 56381
Homologene: 124461
Arfgef2
Name: ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited)
Synonyms: BIG2, E230011G24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 99371
Homologene: 111880
Odf2
Name: outer dense fiber of sperm tails 2
Synonyms: cenexin, MMTEST29
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 18286
HGNC: HGNC:8114
Homologene: 1907
Yars
Name: tyrosyl-tRNA synthetase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 107271
Homologene: 2730
Copa
Name: coatomer protein complex subunit alpha
Synonyms: xenin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12847
HGNC: HGNC:2230
Homologene: 3218
Tmprss6
Name: transmembrane serine protease 6
Synonyms: 1300008A22Rik, matriptase-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 71753
VEGA: 15
Homologene: 12408
Gpsm1
Name: G-protein signalling modulator 1 (AGS3-like, C. elegans)
Synonyms: 1810037C22Rik, Ags3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 67839
Homologene: 16987
Pcbp2
Name: poly(rC) binding protein 2
Synonyms: alphaCP-2, Hnrpx
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 18521
VEGA: 15
HGNC: HGNC:8648
Homologene: 74536
Rnaseh2a
Name: ribonuclease H2, large subunit
Synonyms: 2400006P09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 69724
Homologene: 4664
Naca
Name: nascent polypeptide-associated complex alpha polypeptide
Synonyms: LOC380777, skNAC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 17938
VEGA: 10
HGNC: HGNC:7629
Homologene: 136025
Pnpla3
Name: patatin-like phospholipase domain containing 3
Synonyms: Adpn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 116939
VEGA: 15
Homologene: 11883
Setd1a
Name: SET domain containing 1A
Synonyms: KMT2F
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233904
Homologene: 52251
Slc17a8
Name: solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 8
Synonyms: Vglut3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 216227
Homologene: 13584
Grhl1
Name: grainyhead like transcription factor 1
Synonyms: p70 MGR, Tcfcp2l2, LBP-32, p61 MGR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 195733
VEGA: 12
Homologene: 32219
Serpina10
Name: serine (or cysteine) peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 10
Synonyms: PZI
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 217847
Homologene: 9414
Rasl11b
Name: RAS-like, family 11, member B
Synonyms: 1190017B18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 68939
Homologene: 74291
Capn11
Name: calpain 11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 268958
HGNC: HGNC:1478
Homologene: 21392
Fat3
Name: FAT atypical cadherin 3
Synonyms: LOC234973, D430038H04Rik, LOC382129, 9430076A06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 270120
VEGA: 9
Homologene: 82252
Naip5
Name: NLR family, apoptosis inhibitory protein 5
Synonyms: Birc1e, Naip-rs3, Lgn1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 17951
HGNC: HGNC:7634
Homologene: 113589
Plekhh2
Name: pleckstrin homology domain containing, family H (with MyTH4 domain) member 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 213556
VEGA: 17
Homologene: 35317
Vmn2r6
Name: vomeronasal 2, receptor 6
Synonyms: EG667069, EG620718
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 667069
Homologene: 129754
Kcnn3
Name: potassium intermediate/small conductance calcium-activated channel, subfamily N, member 3
Synonyms: SK3, small conductance calcium-activated potassium channel 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 140493
HGNC: HGNC:6292
Homologene: 20516
Abcb11
Name: ATP-binding cassette, sub-family B (MDR/TAP), member 11
Synonyms: ABC16, PGY4, Lith1, PFIC2, Bsep, sister of P-glycoprotein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 27413
HGNC: HGNC:42
Homologene: 74509
Ceacam12
Name: carcinoembryonic antigen-related cell adhesion molecule 12
Synonyms: Ceacam12-C1, 1600031J20Rik, Ceacam12-C3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 67315
Homologene: 137375
Wnk2
Name: WNK lysine deficient protein kinase 2
Synonyms: 1810073P09Rik, ESTM15, X83337
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 75607
Homologene: 19155
Arhgef5
Name: Rho guanine nucleotide exchange factor (GEF) 5
Synonyms: 2210412D05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 54324
Homologene: 66300
Olfr444
Name: olfactory receptor 444
Synonyms: MOR261-2, GA_x6K02T2P3E9-4602571-4601639
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 258650
Homologene: 88438
Mug1
Name: murinoglobulin 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 17836
Homologene: 136663
Mug2
Name: murinoglobulin 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 17837
Homologene: 136663
Serpina1d
Name: serine (or cysteine) peptidase inhibitor, clade A, member 1D
Synonyms: PI4, Spi1-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 20703
HGNC: HGNC:8941
Homologene: 20103
Urad
Name: ureidoimidazoline (2-oxo-4-hydroxy-4-carboxy-5) decarboxylase
Synonyms: Prhoxnb, EG231903
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231903
Homologene: 82563
Agbl2
Name: ATP/GTP binding protein-like 2
Synonyms: Ccp2, Ccp2, A430081C19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 271813
Homologene: 11715
Kif21b
Name: kinesin family member 21B
Synonyms: N-5 kinesin, 2610511N21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 16565
Homologene: 56868
Cfhr3
Name: complement factor H-related 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 624286
Homologene: 138664
Ptprb
Name: protein tyrosine phosphatase, receptor type, B
Synonyms: VE-PTP, 3230402H02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 19263
VEGA: 10
HGNC: HGNC:9665
Homologene: 2125
Olfr1143
Name: olfactory receptor 1143
Synonyms: MOR177-14, GA_x6K02T2Q125-49303473-49304405
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258290
Homologene: 74079
Plekhg6
Name: pleckstrin homology domain containing, family G (with RhoGef domain) member 6
Synonyms: LOC213522
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 213522
Homologene: 32395
Vmn2r45
Name: vomeronasal 2, receptor 45
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 100042810
Homologene: 113703
B9d1
Name: B9 protein domain 1
Synonyms: Eppb9, B9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 27078
Homologene: 8440
Gm9871
Name: predicted gene 9871
Synonyms: EG207157
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 207157
Olfr1284
Name: olfactory receptor 1284
Synonyms: MOR245-13, GA_x6K02T2Q125-72430580-72431515
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258379
Homologene: 74059
Tktl2
Name: transketolase-like 2
Synonyms: 4933401I19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 74419
Homologene: 69456
Olfr598
Name: olfactory receptor 598
Synonyms: MOR23-4P, MOR23-5, GA_x6K02T2PBJ9-6037823-6038782
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 257975
Homologene: 132401
Lcn3
Name: lipocalin 3
Synonyms: Vnsp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 16820
Homologene: 74958
Vegfc
Name: vascular endothelial growth factor C
Synonyms: VEGF-C
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 22341
Homologene: 3962
Cypt4
Name: cysteine-rich perinuclear theca 4
Synonyms: Ckt1r2, 1700009B20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235067
VEGA: 9
Polh
Name: polymerase (DNA directed), eta (RAD 30 related)
Synonyms: RAD30A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 80905
VEGA: 17
HGNC: HGNC:9181
Homologene: 38189
Zfp131
Name: zinc finger protein 131
Synonyms: 2610109I01Rik, Znf131
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 72465
VEGA: 13
Homologene: 12464
Tmem181a
Name: transmembrane protein 181A
Synonyms: 5930418K15Rik, Gpr178, C76977, Tmem181
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 77106
Homologene: 44787
Tas1r1
Name: taste receptor, type 1, member 1
Synonyms: Gpr70, TR1, T1R1, T1r1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 110326
Homologene: 12888
Try5
Name: trypsin 5
Synonyms: 1810049H19Rik, Tc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 103964
HGNC: HGNC:9486
Homologene: 106639
Gm15931
Name: predicted gene 15931
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Gm6736
Name: predicted gene 6736
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 627189
VEGA: 12
6430562O15Rik
Name: RIKEN cDNA 6430562O15 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 320893
VEGA: 13
Leprel1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Gm26749
Name: predicted gene, 26749
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 136,154,093 bp
  • T to A, chromosome 1 at 139,608,308 bp
  • A to G, chromosome 1 at 172,118,115 bp
  • G to T, chromosome 2 at 25,766,111 bp
  • T to C, chromosome 2 at 26,339,626 bp
  • A to G, chromosome 2 at 29,901,284 bp
  • T to C, chromosome 2 at 69,284,776 bp
  • T to A, chromosome 2 at 87,802,875 bp
  • T to G, chromosome 2 at 90,801,453 bp
  • A to G, chromosome 2 at 111,379,065 bp
  • A to T, chromosome 2 at 166,867,324 bp
  • A to T, chromosome 3 at 64,537,948 bp
  • A to C, chromosome 3 at 89,521,175 bp
  • A to G, chromosome 4 at 129,206,155 bp
  • C to T, chromosome 4 at 141,522,147 bp
  • T to C, chromosome 4 at 152,028,272 bp
  • T to A, chromosome 5 at 74,198,191 bp
  • A to T, chromosome 5 at 147,315,290 bp
  • A to G, chromosome 6 at 41,313,467 bp
  • A to G, chromosome 6 at 42,955,714 bp
  • C to T, chromosome 6 at 43,279,498 bp
  • A to G, chromosome 6 at 101,796,693 bp
  • A to T, chromosome 6 at 121,873,703 bp
  • A to T, chromosome 6 at 122,040,732 bp
  • A to G, chromosome 6 at 125,378,805 bp
  • A to G, chromosome 7 at 4,274,794 bp
  • A to T, chromosome 7 at 8,483,278 bp
  • G to T, chromosome 7 at 18,071,753 bp
  • A to T, chromosome 7 at 103,328,819 bp
  • C to A, chromosome 7 at 127,796,647 bp
  • A to G, chromosome 8 at 54,159,410 bp
  • A to G, chromosome 8 at 66,513,699 bp
  • G to A, chromosome 8 at 84,960,073 bp
  • G to A, chromosome 9 at 16,377,693 bp
  • A to G, chromosome 9 at 24,625,492 bp
  • T to C, chromosome 10 at 89,598,713 bp
  • T to C, chromosome 10 at 116,302,225 bp
  • A to T, chromosome 10 at 128,041,661 bp
  • C to G, chromosome 11 at 61,512,657 bp
  • T to C, chromosome 12 at 13,455,382 bp
  • A to G, chromosome 12 at 24,611,851 bp
  • A to G, chromosome 12 at 103,628,415 bp
  • A to G, chromosome 12 at 103,767,481 bp
  • C to T, chromosome 13 at 49,090,837 bp
  • C to G, chromosome 13 at 99,411,340 bp
  • A to G, chromosome 13 at 100,212,768 bp
  • T to C, chromosome 13 at 119,766,746 bp
  • C to T, chromosome 15 at 78,446,699 bp
  • C to A, chromosome 15 at 84,179,190 bp
  • A to G, chromosome 15 at 102,478,631 bp
  • G to T, chromosome 16 at 26,105,453 bp
  • T to A, chromosome 17 at 6,295,786 bp
  • A to G, chromosome 17 at 45,642,466 bp
  • A to G, chromosome 17 at 46,172,594 bp
  • T to C, chromosome 17 at 74,619,840 bp
  • A to G, chromosome 17 at 84,566,795 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4227 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041047-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.