Strain Name:
C57BL/6J-MtgxR4229Btlr/Mmmh
Stock Number:
041048-MU
Citation ID:
RRID:MMRRC_041048-MU
Other Names:
R4229 (G1), C57BL/6J-MtgxR4229Btlr
Major Collection:

Strain Information

Rabep1
Name: rabaptin, RAB GTPase binding effector protein 1
Synonyms: RAB5 effector protein, neurocrescin, rabaptin-5
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 54189
Homologene: 3451
Gcn1
Name: GCN1 activator of EIF2AK4
Synonyms: G431004K08Rik, GCN1L, Gcn1l1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231659
HGNC: HGNC:4199
Homologene: 5887
Set
Name: SET nuclear oncogene
Synonyms: 2610030F17Rik, StF-IT-1, 5730420M11Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 56086
Homologene: 55707
Zfp148
Name: zinc finger protein 148
Synonyms: ZBP-89, BFCOL1, BERF-1, 2210405J08Rik, beta enolase repressor factor 1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 22661
VEGA: 16
Homologene: 8003
Sec24b
Name: SEC24 homolog B, COPII coat complex component
Synonyms: SEC24
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 99683
Homologene: 55968
Dse
Name: dermatan sulfate epimerase
Synonyms: B130024B19Rik, DS-epi1, Sart2
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 212898
VEGA: 10
Homologene: 8354
Gsap
Name: gamma-secretase activating protein
Synonyms: A530088I07Rik, Pion
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 212167
Homologene: 45504
A2ml1
Name: alpha-2-macroglobulin like 1
Synonyms: Ovos2, BC048546
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232400
Homologene: 45969
Or52e18
Name: olfactory receptor family 52 subfamily E member 18
Synonyms: GA_x6K02T2PBJ9-7589577-7588639, MOR32-8, Olfr670
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 384703
Homologene: 105163
Tmem229a
Name: transmembrane protein 229A
Synonyms: 6332401O19Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 319832
Homologene: 85312
Or4k36
Name: olfactory receptor family 4 subfamily K member 36
Synonyms: MOR248-1, Olfr1280, GA_x6K02T2Q125-72366920-72367837
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258910
Homologene: 121540
Mrpl21
Name: mitochondrial ribosomal protein L21
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 353242
Homologene: 32936
Sspo
Name: SCO-spondin
Synonyms: Scospondin, C79529
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 243369
Homologene: 45453
Pcdh8
Name: protocadherin 8
Synonyms: 1700080P15Rik, Papc
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 18530
HGNC: HGNC:8660
Homologene: 1943
Or4p18
Name: olfactory receptor family 4 subfamily P member 18
Synonyms: Olfr1179, MOR225-2, GA_x6K02T2Q125-49890854-49889931
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258919
Homologene: 74070
Nin
Name: ninein
Synonyms: 3110068G20Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 18080
Homologene: 40632
H2-T24
Name: histocompatibility 2, T region locus 24
Synonyms: H-2T24
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 15042
Homologene: 69019
Dsc3
Name: desmocollin 3
Synonyms: 5430426I24Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 13507
VEGA: 18
HGNC: HGNC:3037
Homologene: 1462
Lipk
Name: lipase, family member K
Synonyms: Lipl2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 240633
VEGA: 19
Homologene: 75666
Aadac
Name: arylacetamide deacetylase
Synonyms: 5033417E09Rik, Aada
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 67758
HGNC: HGNC:17
Homologene: 37436
Etfb
Name: electron transferring flavoprotein, beta polypeptide
Synonyms: 2810441H06Rik, 0610009I16Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 110826
HGNC: HGNC:3482
Homologene: 1503
Or1e1
Name: olfactory receptor family 1 subfamily E member 1
Synonyms: Olfr21, MOR135-11, Olfr20, MTPCR55, MTPCR06, GA_x6K02T2P1NL-3514066-3515010
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 258925
Homologene: 128054
Zfp677
Name: zinc finger protein 677
Synonyms: A830058L05Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 210503
Homologene: 138402
Nxpe4
Name: neurexophilin and PC-esterase domain family, member 4
Synonyms: D930028F11Rik, Fam55d
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 244853
VEGA: 9
Homologene: 83471
Ifi44l
Name: interferon-induced protein 44 like
Synonyms: NS1178, H-28, H28
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 15061
Homologene: 48468
Cdc14a
Name: CDC14 cell division cycle 14A
Synonyms: CDC14A2, CDC14a1, Cdc14, A830059A17Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229776
HGNC: HGNC:1718
Homologene: 75343
Vmn1r223
Name: vomeronasal 1 receptor 223
Synonyms: Gm11330
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 100036518
Homologene: 110880
Plaur
Name: plasminogen activator, urokinase receptor
Synonyms: uPAR, Cd87, u-PAR, urokinase-type plasminogen activator receptor
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 18793
HGNC: HGNC:9053
Homologene: 48120
Ptprv
Name: protein tyrosine phosphatase receptor type V
Synonyms: Esp, OST, mOST-PTP, OST-PTP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 13924
Homologene: 7306
Gm26919
Name: predicted gene, 26919
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Homologene: 134227
Gm16638
Name: predicted gene, 16638
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 102631889
Gm15881
Name: predicted gene 15881
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 100271928
Gm10735
Name: predicted gene 10735
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Gm26672
Name: predicted gene, 26672
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 135,126,207 bp
  • A to G, chromosome 2 at 30,069,519 bp
  • T to A, chromosome 2 at 88,402,883 bp
  • A to G, chromosome 2 at 111,316,336 bp
  • A to G, chromosome 3 at 60,031,813 bp
  • A to G, chromosome 3 at 116,293,764 bp
  • G to T, chromosome 3 at 130,040,719 bp
  • A to T, chromosome 3 at 151,762,877 bp
  • A to G, chromosome 5 at 21,246,977 bp
  • C to A, chromosome 5 at 115,595,319 bp
  • T to A, chromosome 6 at 24,954,832 bp
  • T to C, chromosome 6 at 48,490,934 bp
  • C to T, chromosome 6 at 128,580,386 bp
  • A to T, chromosome 6 at 130,677,345 bp
  • T to C, chromosome 7 at 24,466,783 bp
  • C to A, chromosome 7 at 43,456,560 bp
  • T to C, chromosome 7 at 83,597,402 bp
  • A to T, chromosome 7 at 104,960,594 bp
  • T to C, chromosome 8 at 58,700,184 bp
  • A to G, chromosome 9 at 48,392,822 bp
  • A to T, chromosome 10 at 34,162,744 bp
  • A to G, chromosome 11 at 70,908,434 bp
  • A to T, chromosome 11 at 73,354,232 bp
  • T to C, chromosome 12 at 70,051,210 bp
  • A to T, chromosome 13 at 23,249,415 bp
  • T to C, chromosome 13 at 113,041,209 bp
  • C to T, chromosome 14 at 79,768,896 bp
  • A to G, chromosome 16 at 33,434,763 bp
  • T to C, chromosome 17 at 21,398,282 bp
  • G to A, chromosome 17 at 36,014,829 bp
  • G to A, chromosome 18 at 19,965,821 bp
  • A to G, chromosome 18 at 37,752,231 bp
  • A to G, chromosome 19 at 3,286,901 bp
  • A to G, chromosome 19 at 34,020,287 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4229 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041048-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.